breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 95,913 | G→T | A422S (GCT→TCT) | murF → | D‑alanyl‑D‑alanine‑adding enzyme |
MC JC | 257,908 | Δ776 bp | insB9–[crl] | insB9, insA9, [crl] | |
RA | 372,369 | C→A | D85E (GAC→GAA) | mhpD → | 2‑hydroxypentadienoate hydratase |
RA | 415,246 | C→A | A170S (GCG→TCG) | sbcC ← | ATP dependent, structure specific DNA nuclease ‑ SbcC subunit |
RA | 602,926 | C→A | S323Y (TCT→TAT) | pheP → | phenylalanine:H(+) symporter PheP |
RA | 889,923 | A→G | intergenic (‑104/‑166) | ybjL ← / → ybjM | putative transport protein YbjL/putative inner membrane protein |
RA | 1,639,618 | C→A | G147V (GGA→GTA) | rrrQ ← | Qin prophage; putative lysozyme |
RA | 1,678,604 | G→T | A60S (GCA→TCA) | ydgH → | DUF1471 domain‑containing protein YdgH |
JC JC | 1,879,829 | Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp | coding (115‑120/360 nt) | yeaR ← | DUF1971 domain‑containing protein YeaR |
MC JC | 1,978,503 | Δ776 bp | insB‑5–insA‑5 | insB‑5, insA‑5 | |
RA | 2,088,164 | G→T | S32Y (TCC→TAC) | yeeY ← | putative DNA‑binding transcriptional regulator YeeY |
RA | 2,173,363 | Δ2 bp | pseudogene (915‑916/1358 nt) | gatC ← | galactitol‑specific PTS enzyme IIC component |
RA | 2,355,538 | C→A | F6L (TTC→TTA) | glpC → | anaerobic glycerol‑3‑phosphate dehydrogenase subunit C |
RA | 2,679,795 | C→A | W984L (TGG→TTG) | yphG ← | DUF5107 domain‑containing protein YphG |
RA | 2,804,241 | C→A | A247D (GCT→GAT) | nrdF → | ribonucleoside‑diphosphate reductase 2 subunit beta |
RA | 2,883,898 | G→T | Q81K (CAA→AAA) | casA ← | type I‑E CRISPR system Cascade subunit CasA |
RA | 3,121,993 | C→A | G605W (GGG→TGG) | glcB ← | malate synthase G |
RA | 3,352,471 | C→A | E185D (GAG→GAT) | arcB ← | sensory histidine kinase ArcB |
RA | 3,354,487 | C→T | intergenic (‑437/‑238) | yhcC ← / → gltB | radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB |
RA | 3,445,120 | G→T | T37N (ACT→AAT) | rpsE ← | 30S ribosomal subunit protein S5 |
RA | 3,560,455 | +G | pseudogene (151/758 nt) | glpR ← | DNA‑binding transcriptional repressor GlpR |
RA | 3,815,883 | +C | coding (667/687 nt) | rph ← | truncated RNase PH |
RA | 3,928,161 | C→A | W481C (TGG→TGT) | viaA ← | protein ViaA |
RA | 4,044,084 | C→A | intergenic (+40/‑115) | dsbA → / → yihF | thiol:disulfide oxidoreductase DsbA/uncharacterized protein YihF |
RA | 4,261,710 | C→A | S14R (AGC→AGA) | dusA → | tRNA‑dihydrouridine synthase A |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 3102951 | 3104059 | 1109 | 28 [27] | [27] 28 | [mutY] | [mutY] |
* | * | ÷ | NC_000913 | 3423775–3424234 | 3424525–3424241 | 8–751 | 28 [27] | [20] 29 | [rrfD]–[rrlD] | [rrfD],[rrlD] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 1207790 = | 32 (0.270) | 151 (1.470) | 64/244 | 0.2 | 85.5% | coding (290/630 nt) | ycfK | e14 prophage; protein StfP |
? | NC_000913 | 1209619 = | 23 (0.220) | pseudogene (37/537 nt) | stfE | e14 prophage; putative side tail fiber protein fragment | |||||
* | ? | NC_000913 | = 1207805 | 32 (0.270) | 60 (0.580) | 41/244 | 0.9 | 70.1% | coding (305/630 nt) | ycfK | e14 prophage; protein StfP |
? | NC_000913 | = 1209602 | 23 (0.220) | pseudogene (54/537 nt) | stfE | e14 prophage; putative side tail fiber protein fragment | |||||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 133 (1.180) | 70/268 | 0.2 | 100% | intergenic (+253/‑33) | ychE/insH21 | putative inner membrane protein/IS5 transposase and trans‑activator |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+151/‑484) | insH21/oppA | IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913 | 3363001 = | 206 (1.770) | 218 (1.880) | 85/274 | 0.0 | 67.8% | coding (195/2382 nt) | yhcD | putative fimbrial usher protein YhcD |
? | NC_000913 | 3766087 = | 3 (0.030) | coding (3905/4134 nt) | rhsA | rhs element protein RhsA | |||||
* | ? | NC_000913 | = 3653230 | NA (NA) | 153 (1.310) | 83/276 | 0.1 | 93.9% | noncoding (1/1195 nt) | IS5 | repeat region |
? | NC_000913 | = 3766089 | 10 (0.090) | coding (3907/4134 nt) | rhsA | rhs element protein RhsA |