breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 95,913 G→T A422S (GCT→TCT)  murF → D‑alanyl‑D‑alanine‑adding enzyme
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 372,369 C→A D85E (GAC→GAA mhpD → 2‑hydroxypentadienoate hydratase
RA 415,246 C→A A170S (GCG→TCG)  sbcC ← ATP dependent, structure specific DNA nuclease ‑ SbcC subunit
RA 602,926 C→A S323Y (TCT→TAT)  pheP → phenylalanine:H(+) symporter PheP
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,639,618 C→A G147V (GGA→GTA)  rrrQ ← Qin prophage; putative lysozyme
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,088,164 G→T S32Y (TCC→TAC)  yeeY ← putative DNA‑binding transcriptional regulator YeeY
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,355,538 C→A F6L (TTC→TTA glpC → anaerobic glycerol‑3‑phosphate dehydrogenase subunit C
RA 2,679,795 C→A W984L (TGG→TTG)  yphG ← DUF5107 domain‑containing protein YphG
RA 2,804,241 C→A A247D (GCT→GAT)  nrdF → ribonucleoside‑diphosphate reductase 2 subunit beta
RA 2,883,898 G→T Q81K (CAA→AAA)  casA ← type I‑E CRISPR system Cascade subunit CasA
RA 3,121,993 C→A G605W (GGG→TGG)  glcB ← malate synthase G
RA 3,352,471 C→A E185D (GAG→GAT arcB ← sensory histidine kinase ArcB
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,445,120 G→T T37N (ACT→AAT)  rpsE ← 30S ribosomal subunit protein S5
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,928,161 C→A W481C (TGG→TGT viaA ← protein ViaA
RA 4,044,084 C→A intergenic (+40/‑115) dsbA → / → yihF thiol:disulfide oxidoreductase DsbA/uncharacterized protein YihF
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102951 3104059 1109 28 [27] [27] 28 [mutY] [mutY]
* * ÷ NC_000913 3423775–3424234 3424525–3424241 8–751 28 [27] [20] 29 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =32 (0.270)151 (1.470) 64/244 0.2 85.5% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 23 (0.220)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780532 (0.270)60 (0.580) 41/244 0.9 70.1% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 23 (0.220)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)133 (1.180) 70/268 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =206 (1.770)218 (1.880) 85/274 0.0 67.8% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)153 (1.310) 83/276 0.1 93.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 10 (0.090)coding (3907/4134 nt) rhsA rhs element protein RhsA