breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 355,227 C→A G102G (GGC→GGA codB → cytosine transporter
RA 365,410 G→T P299Q (CCG→CAG)  lacZ ← beta‑galactosidase
RA 561,551 C→A Q619K (CAA→AAA)  sfmD → putative fimbrial usher protein SfmD
MC JC 776,934 Δ90 bp coding (593‑682/1266 nt) tolA → Tol‑Pal system protein TolA
RA 829,104 G→T T290N (ACC→AAC)  ybhG ← HlyD_D23 family protein YbhG
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,791,005 C→A A80A (GCG→GCT ydiU ← UPF0061 family protein YdiU
RA 1,830,164 G→T A201E (GCA→GAA)  astA ← arginine N‑succinyltransferase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,352,538 G→T intergenic (‑164/‑109) glpT ← / → glpA sn‑glycerol 3‑phosphate:phosphate antiporter/anaerobic glycerol‑3‑phosphate dehydrogenase subunit A
RA 2,545,816 C→A S15* (TCG→TAG)  murQ → N‑acetylmuramic acid 6‑phosphate etherase
RA 2,797,105 C→A intergenic (‑77/‑106) yqaE ← / → ygaV Pmp3 family protein/putative DNA‑binding transcriptional regulator YgaV
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,958,523 G→T G185V (GGC→GTC)  ilvC → ketol‑acid reductoisomerase (NADP(+))
RA 4,133,106 C→A A164E (GCG→GAG)  metF → 5,10‑methylenetetrahydrofolate reductase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,492,306 G→T L74M (CTG→ATG)  idnT ← L‑idonate/5‑ketogluconate/gluconate transporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 433029 433133 105 6 [4] [4] 5 nrdR NrdR transcriptional repressor
* * ÷ NC_000913 3103003 3104043 1041 5 [4] [0] 5 [mutY] [mutY]
* * ÷ NC_000913 3423790–3424232 3424558–3424239 8–769 5 [4] [4] 5 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =18 (0.660)26 (1.080) 21/246 0.2 69.4% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 7 (0.290)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780518 (0.660)15 (0.620) 13/246 0.7 56.7% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 7 (0.290)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 3363001 =41 (1.510)44 (1.630) 32/276 0.0 68.4% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)19 (0.700) 14/278 0.8 95.0% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 1 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA