breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 27,977 G→T G229C (GGC→TGC)  rihC → ribonucleoside hydrolase RihC
RA 96,565 C→A G188G (GGC→GGA mraY → phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 354,035 G→T G444W (GGG→TGG)  prpE → propionyl‑CoA synthetase
RA 470,620 C→A R584S (CGC→AGC)  mdlA → ABC transporter family protein MdlA
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 927,511 C→A R562I (AGA→ATA)  cydC ← glutathione/L‑cysteine ABC exporter subunit CydC
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
MC JC 1,736,296 Δ12 bp coding (984‑995/1170 nt) ydhP ← putative transporter YdhP
RA 1,742,680 G→T T188N (ACT→AAT)  ribC ← riboflavin synthase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,338,277 C→T A382T (GCA→ACA)  gyrA ← DNA gyrase subunit A
RA 2,629,867 G→T A26S (GCT→TCT)  yfgH → lipoprotein YfgH
RA 2,684,867 G→T P214Q (CCG→CAG)  glyA ← serine hydroxymethyltransferase
RA 2,715,375 (T)7→6 intergenic (‑55/‑48) yfiE ← / → eamB putative LysR‑type DNA‑binding transcriptional regulator YfiE/cysteine/O‑acetylserine exporter EamB
RA 2,731,060 T→C noncoding (98/1542 nt) rrsG ← 16S ribosomal RNA
RA 3,122,489 G→T I439I (ATC→ATA glcB ← malate synthase G
RA 3,235,293 C→A E195* (GAA→TAA)  rlmG ← 23S rRNA m(2)G1835 methyltransferase
RA 3,245,109 A→C intergenic (‑368/‑115) uxaC ← / → exuT D‑glucoronate/D‑galacturonate isomerase/hexuronate transporter
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,685,332 A→T L96Q (CTG→CAG)  pdeK ← putative c‑di‑GMP phosphodiesterase PdeK
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,995,832 G→T G84C (GGC→TGC)  yigA → conserved protein YigA
RA 3,997,400 C→A T73K (ACG→AAG)  yigB → 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase
RA 4,095,230 G→T S251R (AGC→AGA rhaA ← L‑rhamnose isomerase
RA 4,112,586 C→A A91E (GCG→GAG)  yiiR → DUF805 domain‑containing protein YiiR
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,340,044 G→T P160T (CCA→ACA)  adiA ← arginine decarboxylase, degradative

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196287 1211433 15147 8 [6] [6] 7 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102987 3104043 1057 7 [6] [0] 7 [mutY] [mutY]
* * ÷ NC_000913 3423770–3424230 3424525–3424239 10–756 7 [5] [5] 7 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)57 (1.520) 36/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =72 (1.870)56 (1.460) 41/274 0.1 60.7% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)46 (1.190) 34/276 0.2 95.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA