breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 527,423 C→A A1388E (GCA→GAA)  rhsD → protein RhsD
RA 537,285 C→A L234M (CTG→ATG)  glxR → tartronate semialdehyde reductase 2
RA 637,156 G→T Q155K (CAA→AAA)  ybdO ← putative LysR family DNA‑binding transcriptional regulator YbdO
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,367,291 C→A G208W (GGG→TGG)  pspF ← DNA‑binding transcriptional dual regulator PspF
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,024,541 G→T V192F (GTC→TTC)  rcsA → DNA‑binding transcriptional activator RcsA
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,185,174 G→T A43E (GCG→GAG)  thiM ← hydroxyethylthiazole kinase
RA 2,244,751 C→A intergenic (+5/+27) yeiG → / ← cirA S‑formylglutathione hydrolase/S‑lactoylglutathione hydrolase/ferric dihyroxybenzoylserine outer membrane transporter
RA 2,256,841 G→T I165I (ATC→ATA psuT ← putative pseudouridine transporter
RA 2,339,065 G→T A119E (GCA→GAA)  gyrA ← DNA gyrase subunit A
RA 2,484,466 C→A I31I (ATC→ATA evgS → sensory histidine kinase EvgS
RA 2,795,043 T→C M264T (ATG→ACG)  gabP → 4‑aminobutyrate:H(+) symporter
RA 3,059,555 C→A pseudogene (57/231 nt) yqfE ← conserved protein YqfE
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,355,158 C→A P145Q (CCG→CAG)  gltB → glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
JC JC 3,656,189 IS5 (–) +4 bp coding (101‑104/327 nt) hdeB ← periplasmic acid stress chaperone
RA 3,801,792 C→A G70C (GGT→TGT)  waaJ ← UDP‑glucose:(glucosyl)LPS alpha‑1,2‑glucosyltransferase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,943,340 C→T noncoding (1533/1542 nt) rrsC → 16S ribosomal RNA
RA 4,224,989 C→T R388C (CGT→TGT)  metH → cobalamin‑dependent methionine synthase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,472,003 G→T G131G (GGC→GGA pyrB ← aspartate carbamoyltransferase catalytic subunit
RA 4,574,058 G→T intergenic (+127/‑77) yjiT → / → yjiV putative uncharacterized protein YjiT/putative uncharacterized protein YjiV

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196280 1211430 15151 17 [15] [15] 16 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102950 3104051 1102 16 [15] [15] 18 [mutY] [mutY]
* * ÷ NC_000913 3423772–3424234 3424525–3424239 6–754 16 [15] [13] 17 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3762355–3764074 3765194–3764077 4–2840 16 [15] [15] 16 rhsA rhs element protein RhsA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3363001 =129 (1.780)121 (1.680) 61/274 0.1 65.0% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)76 (1.050) 46/276 0.3 97.4% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.030)coding (3907/4134 nt) rhsA rhs element protein RhsA