breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 93,207 G→T V14V (GTG→GTT murE → UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
RA 197,742 C→A P399P (CCC→CCA rseP → intramembrane zinc metalloprotease
RA 238,189 T→G intergenic (+69/+68) yafT → / ← ykfM lipoprotein YafT/uncharacterized protein YkfM
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 358,111 G→T L194I (CTC→ATC)  cynR ← DNA‑binding transcriptional dual regulator CynR
RA 491,807 C→A G132G (GGC→GGA apt → adenine phosphoribosyltransferase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,504,878 T→A intergenic (+13/+27) insQ → / ← ydcO putative insertion element transposase InsQ/putative transport protein YdcO
RA 1,543,443 C→T S206S (TCG→TCA narU ← nitrate/nitrite transporter NarU
RA 1,613,807 A→T D299E (GAT→GAA sad ← NAD(+)‑dependent succinate semialdehyde dehydrogenase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,920,035 G→T T842T (ACG→ACT yebT → intermembrane transport protein YebT
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,236,974 G→T L260L (CTC→CTA mglC ← D‑galactose/methyl‑galactoside ABC transporter membrane subunit
RA 2,339,206 T→C D72G (GAC→GGC)  gyrA ← DNA gyrase subunit A
RA 2,531,909 C→A R149R (CGC→CGA cysZ → sulfate:H(+) symporter
RA 3,187,862 G→C pseudogene (431/2434 nt) yqiG → putative outer membrane usher protein YqiG
RA 3,206,738 G→T E92D (GAG→GAT ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,306,166 C→A P565P (CCG→CCT deaD ← ATP‑dependent RNA helicase DeaD
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,949,783 (T)7→6 coding (187/1521 nt) yifB ← putative magnesium chelatase YifB
RA 4,005,664 C→A S268* (TCG→TAG)  pldA → outer membrane phospholipase A
RA 4,089,695 C→A S54I (AGT→ATT)  frvR ← putative transcriptional regulator FrvR
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196288 1208598 12311 4 [3] [3] 4 [icd]–[tfaE] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,[tfaE]
* * ÷ NC_000913 1208617 1211418 2802 4 [3] [2] 5 [tfaE]–[icdC] [tfaE],stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3103015 3104047 1033 6 [3] [3] 4 mutY adenine DNA glycosylase
* * ÷ NC_000913 3423782–3424233 3424518–3424238 6–737 4 [3] [3] 5 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)30 (1.060) 21/268 0.3 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =46 (1.570)27 (0.930) 23/274 0.3 53.1% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.070)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)26 (0.890) 21/276 0.4 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 0 (0.000)coding (3907/4134 nt) rhsA rhs element protein RhsA