breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 93,207 | G→T | V14V (GTG→GTT) | murE → | UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase |
RA | 197,742 | C→A | P399P (CCC→CCA) | rseP → | intramembrane zinc metalloprotease |
RA | 238,189 | T→G | intergenic (+69/+68) | yafT → / ← ykfM | lipoprotein YafT/uncharacterized protein YkfM |
MC JC | 257,908 | Δ776 bp | insB9–[crl] | insB9, insA9, [crl] | |
RA | 358,111 | G→T | L194I (CTC→ATC) | cynR ← | DNA‑binding transcriptional dual regulator CynR |
RA | 491,807 | C→A | G132G (GGC→GGA) | apt → | adenine phosphoribosyltransferase |
RA | 889,923 | A→G | intergenic (‑104/‑166) | ybjL ← / → ybjM | putative transport protein YbjL/putative inner membrane protein |
RA | 1,196,220 | C→T | H366H (CAC→CAT) | icd → | isocitrate dehydrogenase |
RA | 1,196,232 | C→T | T370T (ACC→ACT) | icd → | isocitrate dehydrogenase |
RA | 1,196,245 | T→C | L375M (TTA→CTG) | icd → | isocitrate dehydrogenase |
RA | 1,196,247 | A→G | L375M (TTA→CTG) | icd → | isocitrate dehydrogenase |
RA | 1,504,878 | T→A | intergenic (+13/+27) | insQ → / ← ydcO | putative insertion element transposase InsQ/putative transport protein YdcO |
RA | 1,543,443 | C→T | S206S (TCG→TCA) | narU ← | nitrate/nitrite transporter NarU |
RA | 1,613,807 | A→T | D299E (GAT→GAA) | sad ← | NAD(+)‑dependent succinate semialdehyde dehydrogenase |
RA | 1,678,604 | G→T | A60S (GCA→TCA) | ydgH → | DUF1471 domain‑containing protein YdgH |
JC JC | 1,879,829 | Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp | coding (115‑120/360 nt) | yeaR ← | DUF1971 domain‑containing protein YeaR |
RA | 1,920,035 | G→T | T842T (ACG→ACT) | yebT → | intermembrane transport protein YebT |
MC JC | 1,978,503 | Δ776 bp | insB‑5–insA‑5 | insB‑5, insA‑5 | |
RA | 2,173,363 | Δ2 bp | pseudogene (915‑916/1358 nt) | gatC ← | galactitol‑specific PTS enzyme IIC component |
RA | 2,236,974 | G→T | L260L (CTC→CTA) | mglC ← | D‑galactose/methyl‑galactoside ABC transporter membrane subunit |
RA | 2,339,206 | T→C | D72G (GAC→GGC) | gyrA ← | DNA gyrase subunit A |
RA | 2,531,909 | C→A | R149R (CGC→CGA) | cysZ → | sulfate:H(+) symporter |
RA | 3,187,862 | G→C | pseudogene (431/2434 nt) | yqiG → | putative outer membrane usher protein YqiG |
RA | 3,206,738 | G→T | E92D (GAG→GAT) | ttdA → | L(+)‑tartrate dehydratase subunit alpha |
RA | 3,306,166 | C→A | P565P (CCG→CCT) | deaD ← | ATP‑dependent RNA helicase DeaD |
RA | 3,354,487 | C→T | intergenic (‑437/‑238) | yhcC ← / → gltB | radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB |
RA | 3,560,455 | +G | pseudogene (151/758 nt) | glpR ← | DNA‑binding transcriptional repressor GlpR |
RA | 3,815,883 | +C | coding (667/687 nt) | rph ← | truncated RNase PH |
RA | 3,949,783 | (T)7→6 | coding (187/1521 nt) | yifB ← | putative magnesium chelatase YifB |
RA | 4,005,664 | C→A | S268* (TCG→TAG) | pldA → | outer membrane phospholipase A |
RA | 4,089,695 | C→A | S54I (AGT→ATT) | frvR ← | putative transcriptional regulator FrvR |
RA | 4,261,710 | C→A | S14R (AGC→AGA) | dusA → | tRNA‑dihydrouridine synthase A |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 1196288 | 1208598 | 12311 | 4 [3] | [3] 4 | [icd]–[tfaE] | [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,[tfaE] |
* | * | ÷ | NC_000913 | 1208617 | 1211418 | 2802 | 4 [3] | [2] 5 | [tfaE]–[icdC] | [tfaE],stfE,pinE,mcrA,[icdC] |
* | * | ÷ | NC_000913 | 3103015 | 3104047 | 1033 | 6 [3] | [3] 4 | mutY | adenine DNA glycosylase |
* | * | ÷ | NC_000913 | 3423782–3424233 | 3424518–3424238 | 6–737 | 4 [3] | [3] 5 | [rrfD]–[rrlD] | [rrfD],[rrlD] |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 30 (1.060) | 21/268 | 0.3 | 100% | intergenic (+253/‑33) | ychE/insH21 | putative inner membrane protein/IS5 transposase and trans‑activator |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+151/‑484) | insH21/oppA | IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913 | 3363001 = | 46 (1.570) | 27 (0.930) | 23/274 | 0.3 | 53.1% | coding (195/2382 nt) | yhcD | putative fimbrial usher protein YhcD |
? | NC_000913 | 3766087 = | 2 (0.070) | coding (3905/4134 nt) | rhsA | rhs element protein RhsA | |||||
* | ? | NC_000913 | = 3653230 | NA (NA) | 26 (0.890) | 21/276 | 0.4 | 100% | noncoding (1/1195 nt) | IS5 | repeat region |
? | NC_000913 | = 3766089 | 0 (0.000) | coding (3907/4134 nt) | rhsA | rhs element protein RhsA |