Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A25 F999 I0 R1
|
111 |
24.8 |
2365438 |
97.5% |
2306302 |
48.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,093,223 |
A→G |
V72V (GTT→GTC) |
rhaM ← |
L‑rhamnose mutarotase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,093,223 | 0 | A | G | 100.0%
| 185.2
/ NA
| 47 | V72V (GTT→GTC) | rhaM | L‑rhamnose mutarotase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (30/17); total (30/17) |
GTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAA > NC_000913/4093178‑4093266
|
gTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACa < 1:1539679/49‑1 (MQ=255)
gTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACa < 1:1464041/49‑1 (MQ=255)
gCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAg < 1:2009032/49‑1 (MQ=255)
gggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTg < 1:1926132/49‑1 (MQ=255)
gggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTg > 1:306406/1‑49 (MQ=255)
ggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGc > 1:1631505/1‑49 (MQ=255)
cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg > 1:2274748/1‑49 (MQ=255)
cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg > 1:1002985/1‑49 (MQ=255)
cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg > 1:93099/1‑49 (MQ=255)
tAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGc < 1:219491/49‑1 (MQ=255)
aaCATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGc > 1:919216/1‑48 (MQ=255)
cATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAAc > 1:2034273/1‑49 (MQ=255)
tCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg > 1:2276177/1‑49 (MQ=255)
tCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg < 1:1595195/49‑1 (MQ=255)
cGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg > 1:315602/1‑48 (MQ=255)
gTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAt > 1:269714/1‑49 (MQ=255)
gTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAt > 1:628227/1‑49 (MQ=255)
tCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAtt > 1:1189609/1‑49 (MQ=255)
tCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAtt > 1:919856/1‑49 (MQ=255)
cATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc > 1:2028741/1‑49 (MQ=255)
atatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTcc < 1:1104115/49‑1 (MQ=255)
atatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc > 1:2158203/1‑48 (MQ=255)
tatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCa < 1:382693/49‑1 (MQ=255)
atatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAg > 1:1485305/1‑49 (MQ=255)
atatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAg > 1:1415617/1‑49 (MQ=255)
tatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAg < 1:1360339/48‑1 (MQ=255)
ttCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGtt > 1:472389/1‑49 (MQ=255)
ttCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGtt > 1:1494746/1‑49 (MQ=255)
ttCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGtt > 1:1205155/1‑49 (MQ=255)
tCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGtt < 1:208837/48‑1 (MQ=255)
tCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGtt < 1:289553/48‑1 (MQ=255)
ccaccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttct > 1:237126/1‑49 (MQ=255)
ccaccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttc < 1:233060/48‑1 (MQ=255)
accaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttcttc < 1:1170245/49‑1 (MQ=255)
accaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt > 1:1628389/1‑48 (MQ=255)
ccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt > 1:1580694/1‑47 (MQ=255)
ccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt > 1:202126/1‑47 (MQ=255)
aaCGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt > 1:2334942/1‑45 (MQ=255)
aaCGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt > 1:1317126/1‑45 (MQ=255)
aaCGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt > 1:1156021/1‑45 (MQ=255)
aaCGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGa < 1:293328/49‑1 (MQ=255)
aCGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAt > 1:2272943/1‑49 (MQ=255)
cGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAtt < 1:1376300/49‑1 (MQ=255)
cGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAtt < 1:2189811/49‑1 (MQ=255)
gTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAtt > 1:2318023/1‑48 (MQ=255)
ttGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCa < 1:2006948/49‑1 (MQ=255)
tGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCaa > 1:1103497/1‑49 (MQ=255)
|
GTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAA > NC_000913/4093178‑4093266
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
