Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A15 F999 I0 R1
|
92 |
59.4 |
4455785 |
96.3% |
4290920 |
49.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,093,223 |
A→G |
V72V (GTT→GTC) |
rhaM ← |
L‑rhamnose mutarotase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,093,223 | 0 | A | G | 100.0%
| 258.5
/ NA
| 65 | V72V (GTT→GTC) | rhaM | L‑rhamnose mutarotase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (34/31); total (34/31) |
GGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAATCT > NC_000913/4093176‑4093269
|
gggTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGAc < 1:51250/50‑1 (MQ=255)
gggTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGAc < 1:1439735/50‑1 (MQ=255)
gTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACAt < 1:3536594/50‑1 (MQ=255)
gTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACAt > 1:912609/1‑50 (MQ=255)
gTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACAt > 1:811275/1‑50 (MQ=255)
gTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACAt < 1:770840/50‑1 (MQ=255)
ttAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACAt > 1:2691095/1‑49 (MQ=255)
tAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACAt > 1:896584/1‑48 (MQ=255)
gCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAg < 1:1379643/49‑1 (MQ=255)
gCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAg < 1:3502795/49‑1 (MQ=255)
gCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGt < 1:2045557/50‑1 (MQ=255)
gggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGc > 1:3267986/1‑50 (MQ=255)
gggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGc > 1:553152/1‑50 (MQ=255)
gggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGc < 1:3126304/50‑1 (MQ=255)
ggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCt < 1:142072/50‑1 (MQ=255)
cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTgg > 1:3206414/1‑50 (MQ=255)
cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTgg < 1:3707761/50‑1 (MQ=255)
cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTgg > 1:2377771/1‑50 (MQ=255)
aTAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTgg > 1:1031057/1‑49 (MQ=255)
aTAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGc < 1:4337670/50‑1 (MQ=255)
tAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGc > 1:3600562/1‑49 (MQ=255)
aaCATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCaa > 1:429018/1‑50 (MQ=255)
aCATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCaa < 1:3876177/49‑1 (MQ=255)
aCATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAAc > 1:1185963/1‑50 (MQ=255)
aCATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAAc > 1:1135196/1‑50 (MQ=255)
cATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCGAcc < 1:425886/50‑1 (MQ=255)
cATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAAcc < 1:4439106/50‑1 (MQ=255)
cATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAAcc < 1:3109228/50‑1 (MQ=255)
aTCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg > 1:3394621/1‑50 (MQ=255)
aTCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg > 1:1702124/1‑50 (MQ=255)
aTCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg < 1:4227305/50‑1 (MQ=255)
ggTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAt > 1:2439963/1‑50 (MQ=255)
gTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAtt < 1:1933162/50‑1 (MQ=255)
tCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc > 1:3772517/1‑50 (MQ=255)
atatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTcc < 1:2191852/49‑1 (MQ=255)
atatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc > 1:3178979/1‑48 (MQ=255)
atatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCa < 1:1012869/50‑1 (MQ=255)
tatTTCCACCAACGTTGGCAGACCTCAGTGCTGGCAACCGCATTCCAGCg > 1:653164/1‑50 (MQ=39)
tatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCg > 1:2892601/1‑50 (MQ=255)
tatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCg < 1:2368166/50‑1 (MQ=255)
tttCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGtt < 1:399806/50‑1 (MQ=255)
tttCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGtt < 1:4247619/50‑1 (MQ=255)
tCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttct > 1:3622300/1‑50 (MQ=255)
tCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttc < 1:2038649/49‑1 (MQ=255)
ccaccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt > 1:1485197/1‑50 (MQ=255)
caccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt > 1:3754043/1‑49 (MQ=255)
accaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt > 1:3204649/1‑48 (MQ=255)
accaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt > 1:3720378/1‑48 (MQ=255)
accaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCa < 1:3295043/50‑1 (MQ=255)
ccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAg < 1:2510795/50‑1 (MQ=255)
ccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAg < 1:2550961/50‑1 (MQ=255)
caACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGa < 1:627233/50‑1 (MQ=255)
aaCGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAt < 1:4077651/50‑1 (MQ=255)
aaCGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAt > 1:1073185/1‑50 (MQ=255)
cGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAtt > 1:3959022/1‑49 (MQ=255)
gTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTc > 1:3361203/1‑49 (MQ=255)
gTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTc > 1:3517915/1‑49 (MQ=255)
ttGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCaa > 1:2357394/1‑50 (MQ=255)
tGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAt > 1:2706367/1‑50 (MQ=255)
tGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAt > 1:94190/1‑50 (MQ=255)
ggCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAtc > 1:2313507/1‑50 (MQ=255)
ggCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAt < 1:4265959/49‑1 (MQ=255)
gCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAtct < 1:4099102/50‑1 (MQ=255)
gCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAtct > 1:3426946/1‑50 (MQ=255)
gCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAtct < 1:2843366/50‑1 (MQ=255)
|
GGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAATCT > NC_000913/4093176‑4093269
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
