Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F258 I0 R1 432 103.0 5191697 92.7% 4812703 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,370,616 A→G 60.2% intergenic (‑204/‑72) yjeH ← / → groS putative transporter/Cpn10 chaperonin GroES, small subunit of GroESL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,370,6160AG60.2% 84.0 / 115.7 118intergenic (‑204/‑72)yjeH/groSputative transporter/Cpn10 chaperonin GroES, small subunit of GroESL
Reads supporting (aligned to +/- strand):  ref base A (20/27);  new base G (36/35);  total (56/62)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 118 total aligned reads displayed.

TGAATCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGAT  >  NC_000913/4370523‑4370711
                                                                                             |                                                                                               
tGAATCAGTGTTTTCACCCGATTTTGTGCTGATCAGAACTTTTTTTCTTTTTCCCCCTTGAAGGGGCGATGCCTCATCCCCATTTCTCTGGTCGCCAGcc                                                                                           <  1:1846839/100‑1 (MQ=255)
  ggtgaatcagggttttcacccgattttgtgctgatcagaattttttttctttttcccccttgaaggggcgaagactcatccccatttGGTCACCAGCCgg                                                                                         >  2:1899179‑M2/88‑100 (MQ=255)
   atcaatgTTTTCACCCGATTTTGGGCTGATCAGAATTTTTTTTCTTTTTCCCCCGTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCggg                                                                                        <  1:906749/94‑1 (MQ=255)
   aTCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCggg                                                                                        <  2:598691/100‑1 (MQ=255)
   aTCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGAGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCggg                                                                                        >  2:2468630/1‑100 (MQ=255)
     gaatcagggttttcacccgattttgtgctgatcagaattttttttctttttcccccttgaaggggcgaagcctcatccccatttGGTCACCAGCCGGGaa                                                                                      <  2:803290‑M2/16‑1 (MQ=255)
     cAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGaa                                                                                      >  1:151329/1‑100 (MQ=255)
      aatcagggttttcaccagattttgtgctgatcagaattttttttcgttttcccccttgaaggggcgaagcctcatccccatttGGTCACCAGCCGGGaaa                                                                                     <  1:929387‑M2/17‑1 (MQ=255)
      aGGGTTTTCGCCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGaaa                                                                                     <  1:2322407/100‑1 (MQ=255)
      aGGGTTTTCACCCGATTTTGTGCTGCTCAGAATTTTTTTTCTTTTTCCCCCTTTAAGGGGCGAAGCCTCCTCCCCATT‑‑‑‑TGGTCACCAGCCGGGAAACCAc                                                                                 >  2:1175661/1‑100 (MQ=255)
      aGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCTCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGaaa                                                                                     >  1:1470613/1‑100 (MQ=255)
      aGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGaaa                                                                                     <  2:773345/100‑1 (MQ=255)
      aGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTATGGTCGCCAGCCGGGaaa                                                                                     >  2:1376150/1‑100 (MQ=255)
         gTTTTCACCCGATTTTGTGCTGATCAGAATTTTGTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCa                                                                                  <  2:2116822/100‑1 (MQ=255)
          agggttttcacccgattttgtgctgatcagaattttttttctttttcccccttgaaggggcgaagcctcatccccatttGGTCACCAGCCGGGAAACCAc                                                                                 >  2:2206655‑M2/80‑100 (MQ=255)
           tttCACCCGATTTTGTGCTGGTCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCTTCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACg                                                                                <  1:1306376/100‑1 (MQ=255)
           gggttttcacccgattttgtgctgatcagaattttttttctttttcccccttgaaggggcgaagcctcatccccatttGGTCACCATCCGGGAAAccatg                                                                                <  2:1755666‑M2/22‑3 (MQ=255)
            ttCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGt                                                                               <  2:2064005/100‑1 (MQ=255)
             tCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTa                                                                              >  1:657450/1‑100 (MQ=255)
             tCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTa                                                                              <  2:1379003/100‑1 (MQ=255)
              gttttcacccgattttgtgctgatcagaatttttttctttttcccccttgaaggggcgaagcctcatccccatttGGTCACCAGCCGGGAAACCACGTaa                                                                             >  2:1007773‑M2/76‑100 (MQ=255)
               actcGATTTAGTGCTGATCAGAATTTTTTTTCATTTTCCGCCTAAAAGGGGCGTAGCCTCATCCCCATTTC‑‑‑‑GTCACCAGCCGGAAAACCACGTAAGca                                                                          >  1:454552/4‑97 (MQ=255)
               aCCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAg                                                                            >  2:1290374/1‑100 (MQ=255)
                cccGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGc                                                                           >  2:2538221/1‑100 (MQ=255)
                      tttGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTGGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGc                                                                     <  1:720524/100‑1 (MQ=255)
                       gattttgtgctgatcagaattttttttctttttcccccttgaaggggcgaagcctcatccccatttGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCg                                                                    <  1:1402076‑M2/34‑1 (MQ=255)
                        tgtgCTGGTCAGAATTTTTTTGTTTTTTCCCCTTTTGAGGGGCGATGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCg                                                                    <  1:1179869/99‑1 (MQ=255)
                           gCTGATCAGAATTTTTTTTTTTTTTTTTTCTTGAAGGAGAGAAGATTCATCCTCATTTC‑‑‑‑GTTACTAGCGGGGAAACCTCGTAATTTCCGTCCTCACCCAt                                                            >  1:2431564/1‑100 (MQ=255)
                           gCTGATCAGAATTTTTTTTCTTTTTTCCTCTTGAAGGGGCAACGCCTTATCCCCATT‑‑‑‑TGGTCACCACCCGCGAGACTACGTACGCTCCGGCGTCCCCCAt                                                            >  1:45823/1‑100 (MQ=255)
                           gCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCAc                                                                <  2:1452903/100‑1 (MQ=255)
                           gCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCAc                                                                >  2:2126607/1‑100 (MQ=255)
                           gCTGATCAGAATTTTTTTTCTTTTTCCCCATGGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCa                                                                 <  1:766543/99‑1 (MQ=255)
                            cTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCAcc                                                               <  2:122627/100‑1 (MQ=255)
                            cTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCAcc                                                               <  2:1675383/100‑1 (MQ=255)
                             tGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCAccn                                                              <  1:118390/100‑2 (MQ=255)
                               gctgatcagaattttttttctttttcccccttgaaggggcgaagcctcatccccatttGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCAt                                                            <  2:1671043‑M2/42‑1 (MQ=255)
                                tCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATa                                                           <  2:2056127/100‑1 (MQ=255)
                                tCAGAATTTTTTTTCTTTTGCCGCCTGGGAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCAccc                                                              <  1:1161105/97‑1 (MQ=255)
                                  aGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAAc                                                         <  2:763722/100‑1 (MQ=255)
                                    aaTTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAg                                                       >  2:2252803/1‑100 (MQ=255)
                                      ttttttttCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGAt                                                     <  2:1231931/100‑1 (MQ=255)
                                       tttttttCTTTTCCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGAt                                                     <  1:642194/99‑1 (MQ=255)
                                       gaattttttttctttttcccccttgaaggggcgaagcctcatccccatttGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATa                                                    <  2:473137‑M2/50‑1 (MQ=255)
                                        ttttttCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTTGTCGCCCGCCGGGAAACGACGTAAGCTCCGGCGTCACCCATAACAGATAc                                                   >  1:1092182/1‑100 (MQ=255)
                                          ttttcttttcaCCAAATGAAGGGGCGAAGCCTCCTCCCCCTTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACg                                                  <  1:1363533/99‑1 (MQ=255)
                                           tttctttttcCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAAGCACGTAAGCTCCGGCGTCACCCATAACAGATACGGa                                                >  2:1158427/1‑100 (MQ=255)
                                           tttctttgtcCACCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGa                                                >  1:1362180/1‑100 (MQ=255)
                                            ttttttctttttcccccttgaaggggcgaagcctcatccccatttGGTCACCAGCTGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGAc                                               <  2:1012271‑M2/55‑1 (MQ=255)
                                            ttttttctttttcccccttgaaggggcgaagcctcatccccatgtGGTCACCAGCCGGGAAACCGCGTAAGCTCCGGCGTCACCCATATCAGATACGGAc                                               <  1:1175657‑M2/55‑1 (MQ=255)
                                            ttctttttcCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGAc                                               <  2:460822/100‑1 (MQ=255)
                                             tctttttcCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACt                                              <  1:760407/100‑1 (MQ=255)
                                              ctttttcCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACtt                                             >  2:2220123/1‑100 (MQ=255)
                                              ctttttcCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACtt                                             >  2:2440880/1‑100 (MQ=255)
                                               tttttcCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACttt                                            >  1:1306225/1‑100 (MQ=255)
                                               tttttcCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACttt                                            >  1:736070/1‑100 (MQ=255)
                                                  ctttttcccccttgaaggggcgaagcctcatccccatttGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTtctc                                         <  1:1317979‑M2/61‑1 (MQ=255)
                                                   tttttcccccttgaaggggcgaagcctcatccccatttGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCa                                        <  2:1104370‑M2/62‑1 (MQ=255)
                                                   tcCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCa                                        <  2:657453/100‑1 (MQ=255)
                                                     ccccTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTACGCTCCGGCGTCCCCCATCACAGATACGGACTTTCTCaaa                                      >  1:185905/1‑100 (MQ=255)
                                                     ccccTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCaaa                                      <  2:858999/100‑1 (MQ=255)
                                                     ccccTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCaaa                                      >  1:827667/1‑100 (MQ=255)
                                                     ccacTTGAAGGGGAGAAGTCTCATCCCCATTGCTCTGCTCGCCAGCGGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCaa                                       >  1:363963/4‑99 (MQ=255)
                                                      ctcTTGGAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAAGAGATACGGACTTTCTCAAAg                                     <  1:757124/98‑1 (MQ=255)
                                                      ccgttGAAGGGGCGATGCCTCATCCCCATTTCTCTGGTCGCCAGGCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCaa                                       <  1:2531791/95‑1 (MQ=255)
                                                      cccTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAg                                     <  2:515313/100‑1 (MQ=255)
                                                      cccTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGCCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTACTCAAAg                                     >  1:1387628/1‑100 (MQ=255)
                                                        ctggAAGGGGCGAAGCCTCATCCCCATTTCTCGGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAg                                     <  1:1113299/95‑1 (MQ=255)
                                                        cccccttgaaggggcgaagcctcatccccatttGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGga                                   >  1:1335299‑M2/34‑100 (MQ=255)
                                                        cTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCaacgag                                   >  2:757127/1‑96 (MQ=255)
                                                          tGAAGGGGCGAAGCCTCATCACCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGgaga                                 >  1:1587929/1‑100 (MQ=255)
                                                           gAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGagag                                >  1:246924/1‑100 (MQ=255)
                                                           gAAGAGGCGAAGCATCATCCCCATTTCTCTGGTCGCCAGCCGGGAATCTACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGagag                                <  1:1784276/100‑1 (MQ=255)
                                                           ccttgaaggggcgaagcctcatccccatttGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGagag                                <  1:1680673‑M2/70‑1 (MQ=255)
                                                            aaGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGt                               >  1:86836/1‑100 (MQ=255)
                                                             aGGGGCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGtt                              >  1:1300325/1‑100 (MQ=255)
                                                               gggCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTAt                            <  2:1194915/100‑1 (MQ=255)
                                                                ggCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATc                           >  1:1316760/1‑100 (MQ=255)
                                                                ggCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATc                           <  1:47110/100‑1 (MQ=255)
                                                                ggCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATc                           <  2:734664/100‑1 (MQ=255)
                                                                ggCGAAGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATc                           <  1:974485/100‑1 (MQ=255)
                                                                    aaGCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATg                       <  1:1307122/100‑1 (MQ=255)
                                                                      gCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGaa                     >  1:1958243/1‑100 (MQ=255)
                                                                      gCCTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGaa                     >  2:1428211/1‑100 (MQ=255)
                                                                       gaagcctcatccccatttGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAat                    >  1:706591‑M2/19‑100 (MQ=255)
                                                                       ccTCATCCCCATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAat                    >  2:1974452/1‑100 (MQ=255)
                                                                        cTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTATCAAAGGAGAGTTATCAATGAata                   >  2:2216356/1‑100 (MQ=255)
                                                                            tCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTTTCAAAGGAGAGTTATCAATGAATATTCg               <  2:236024/100‑1 (MQ=255)
                                                                             ccccATTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGt              >  1:48227/1‑100 (MQ=255)
                                                                               ccATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTTTCAAAGGAGAGTTATCAATGAATATTCGTcc            >  1:437310/1‑100 (MQ=255)
                                                                               atccccatttGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTcc            >  1:1792280‑M2/11‑100 (MQ=255)
                                                                               atccccatttGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTcc            <  2:1927662‑M2/90‑1 (MQ=255)
                                                                                 ccccatgtGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCAt          <  2:2472624‑M2/92‑1 (MQ=255)
                                                                                 aTTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCAt          <  2:2183125/100‑1 (MQ=255)
                                                                                 aTTTCTCTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCAt          <  2:1797514/100‑1 (MQ=255)
                                                                                 aTTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTTTCAAAGGAGAGTTATCAATGAATATTCGTCCAt          >  2:2505035/1‑100 (MQ=255)
                                                                                    tctcTGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGc       >  1:1458175/1‑100 (MQ=255)
                                                                                     ctctGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGc       <  1:1448406/99‑1 (MQ=255)
                                                                                       ctGGTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATg    >  2:1175412/1‑100 (MQ=255)
                                                                                       ctGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATg    >  1:1955568/1‑100 (MQ=255)
                                                                                         ggTCGCCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGAt  <  1:1643843/100‑1 (MQ=255)
                                                                                             |                                                                                               
TGAATCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGAT  >  NC_000913/4370523‑4370711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A