Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F67 I1 R1	23	41.0	1710050	87.8%	1501423	142.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	97.4%	107.9 / NA	40	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (19/19); minor base G (0/1); total (19/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGG  >  NC_000913/696336‑696606

aTCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCCCGGAATGCCGGAATTAG‑AATCCg                                                                                                                                  >  2:850637/1‑143 (MQ=255)

    gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGc                                                                                                                              <  2:76838/143‑1 (MQ=255)

              gATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCTGAATGCAGGAATTAG‑AATCAGGGGCCTTACAGCtt                                                                                                                    >  1:678442/1‑143 (MQ=38)

                tAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGCATGCCGGACTTAG‑AATCCGGTGCCTTCCCGATTgg                                                                                                                  >  2:322231/1‑143 (MQ=38)

                 aaGGCGTGTGAACATCGGATTCGACATTGTATTAACGCAGAAAAGCATAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGc                                                                                                                 <  2:805613/143‑1 (MQ=25)

                   gACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGa                                                                                                               <  1:178496/143‑1 (MQ=255)

                      gtgtCAACATCGCATTCGACATTGAATGAACGCAAAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATAc                                                                                                            <  2:193138/143‑1 (MQ=255)

                         tCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGCAATGCCGGAATTAG‑AATCCGGTGCCATACAGCTTGGCGATAcgcc                                                                                                         >  1:765787/1‑143 (MQ=37)

                           aaCATCGCATTCGACATTGAATGAACGCAGAATAGCAAAAAGCTCGACGAAGCGAGCTTTTTTAATGTGGCTGGGTTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTGACCGCTTGGCGATACCCTaa                                                                                                       <  2:439986/143‑1 (MQ=25)

                           aaCATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGAGACCCGaa                                                                                                       >  2:489395/1‑143 (MQ=255)

                                        ataTTGAGTGAACGCAGAAAAGCAAATAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACTTCGTAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTa                                                                                          <  2:521062/141‑1 (MQ=25)

                                        aCATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGCTACCCCCACTGAGTGcctttc                                                                                          >  2:237563/1‑138 (MQ=25)

                                            tgaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg                                                                                      <  1:827784/143‑1 (MQ=255)

                                            tgaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGTTGCCTTTACAAg                                                                                      >  1:703113/1‑143 (MQ=21)

                                            tgaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACACCAACAGGGTGAACTTAAAAg                                                                                      >  1:839675/1‑143 (MQ=25)

                                            ggaaggaaCGGAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg                                                                                      <  1:497668/142‑1 (MQ=255)

                                              aatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATAc                                                                                                            <  1:486341/119‑1 (MQ=255)

                                              aatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATAc                                                                                                            >  2:486341/1‑119 (MQ=255)

                                              aatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCTCTTGCAAGGt                                                                                    >  1:294604/1‑143 (MQ=255)

                                                tgaaCGCAGATAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTaa                                                                                  <  2:813155/143‑1 (MQ=255)

                                                       aggaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                           <  2:226407/140‑1 (MQ=255)

                                                       aGAAAAGCAAAAAGTTTGTTTTAGTGAGTTTTTTTAAAGTGGCTGGGGGACGAGGATTCGAAACTAGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                           <  1:58949/143‑1 (MQ=18)

                                                       aGAAAAGCAAAAAGCTCGTCGATGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGAGTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                           <  1:479669/143‑1 (MQ=255)

                                                              cAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGCGTGCACTTCCAAGGTAAGCGTCTTGACTaaa                                                                    >  2:144217/1‑143 (MQ=255)

                                                                     cTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACACGGTAAGCGTCTTGAATAAATTGGCTg                                                             >  1:297027/1‑143 (MQ=255)

                                                                      tCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGACTgg                                                            >  1:283301/1‑143 (MQ=255)

                                                                       cGCCGAAGCGAGCTTTTTTAATGGGGCGGGGGGACGAGGATTCGAACCTCGGAAGGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTggg                                                           <  1:322231/143‑1 (MQ=25)

                                                                        gCCGAAGCGGGCTGTGTTAGTGTGGCTGTGGTCCGAGGATTCGAGCCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTgggg                                                          <  2:519214/143‑1 (MQ=21)

                                                                                gAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAGCAATGG‑TGCAGTAAGCTTGTGCCCTTCATCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGa                                                  <  2:600811/143‑1 (MQ=255)

                                                                                gAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGAATAAAATAAATTGGCTGGGGTACGAGGa                                                  >  1:256544/1‑143 (MQ=21)

                                                                                  gCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAtt                                                >  1:251974/1‑143 (MQ=255)

                                                                                    ttttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCg                                              >  1:629699/1‑143 (MQ=255)

                                                                                      gtttAATGTGGATGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGaa                                            <  1:726420/142‑1 (MQ=255)

                                                                                           gtttagCTGGCGGACGATGATTCCAACCTCGGAAGGCCAGAATTAG‑CATCCGGTGCCTTACCGCTTGGCCATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCg                                       <  1:589737/138‑1 (MQ=25)

                                                                                                      gTACGAGGACTCGGACCTCGGAGTGCCGGAATTAG‑GATCCGGTGCCTTACCGCTTGGCGATACTCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGaa                            <  2:709506/143‑1 (MQ=37)

                                                                                                             gagtCGAACCTCGGGAGGCCGGAATGAG‑AACCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACGAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                     <  2:727070/140‑1 (MQ=38)

                                                                                                                              gCCGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAGGCGTCTTGAATAAATTGGCTGGGGTACGTGGATTCGAACCTCGGAATGCTGGAATCAGAATCCGGTACATTACAGCtt    >  1:532945/1‑143 (MQ=38)

                                                                                                                                cgaaaTTAG‑AATCCGGCGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg  <  1:601367/140‑1 (MQ=255)

                                                                                                                                cGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTTGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg  >  1:161147/1‑143 (MQ=255)

                                                                                                                                cGGAATTAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGATCCGCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg  >  1:710733/1‑143 (MQ=255)

ATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAG‑AATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGG  >  NC_000913/696336‑696606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A