Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F51 I0 R1
|
32 |
33.5 |
1326866 |
89.5% |
1187545 |
142.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
696,470 |
C→T |
100% |
noncoding (35/75 nt) |
glnX ← |
tRNA‑Gln |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 696,470 | 0 | C | T | 100.0%
| 48.0
/ ‑6.7
| 25 | noncoding (35/75 nt) | glnX | tRNA‑Gln |
| Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (9/12); minor base G (1/1); total (10/15) |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGG > NC_000913/696338‑696606
|
ccGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGt > 1:391266/1‑143 (MQ=255)
aCATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAAAGTGGCTAGGGTACGAGGATTCGAACCTCGGAAAGCCGGACTGAGAATCCGGGGcc > 2:415126/1‑143 (MQ=38)
tacgACTTGTCAACATCGCATTCCACATTGAATGAGTGCAGAAAAGCAAGAAGCTCGCCGAAGCGAGCCTTTTTAATGTGGCTGTGGTACGAGGATTCGAACCTCGCAATGCCGGAATAAGAATCCAGTTGGTTACCGTTTgg < 2:438008/140‑1 (MQ=25)
tCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAACGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGAtgcgcc > 1:647222/1‑138 (MQ=255)
cGCATGCGACATTGAATGAACGCAGAAAAGCAAAGAGCTCGCCGAAGCGAGCTTTTTTAATGTGTCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTggg < 2:532809/143‑1 (MQ=255)
cGCATCCGACATTGAATGATCGCAGAAGAGCCAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGCACGAGGAGTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTggg < 2:434437/143‑1 (MQ=21)
aTTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGATGCCGTACCGCTTGGCGAGACCCCAACCGGCTGCGCtttcc > 1:89966/1‑140 (MQ=21)
tgggaagaaCGAAGAACAGCAAAAAGCTGGCCGAAGTGAGCTCTTTTAACGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCGTGGCGATACCCCAACTGGGTGCACTTACaa < 1:535920/137‑1 (MQ=18)
cGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTACGCGt > 1:651276/1‑143 (MQ=255)
gCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGCGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCCACTGGGTGCACTTACAAGGTCAGCGta > 1:474250/1‑142 (MQ=255)
cAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCt < 1:161569/143‑1 (MQ=255)
aGAAAAGGAATAAGCTCGCCGAAGCGAGGTTTTGTACTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt < 2:285185/143‑1 (MQ=21)
aaagaGCCCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAt < 2:352600/143‑1 (MQ=255)
aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAt < 1:431575/143‑1 (MQ=255)
aaGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTagg > 1:35693/1‑141 (MQ=255)
gtgGCGGGGGTACGAGGACTCGAGCCGCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCGACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGa < 2:381207/143‑1 (MQ=37)
tgGCTGGGGTAGGAGGATTCGAACCTCGGAATGCCTGAATGATAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGaa < 1:273901/143‑1 (MQ=255)
tGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGcc < 1:398112/143‑1 (MQ=255)
gATTCGAACCTCGGAATGCCGGAATTAGACTCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAACGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt < 1:201060/143‑1 (MQ=255)
ttCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGTTACGAGGATACGAACCTCGGAGTGCCGGCATCAGCATcc > 1:210279/1‑143 (MQ=37)
aaCCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg < 1:533245/143‑1 (MQ=255)
cGGAATGCCGTATTTATATTCCGGTGCCTTATCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTa < 1:379466/143‑1 (MQ=37)
gAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTATGCGTCTTGAATATATTGGCTGGGGTACGAGGATTCGACGCTCGGCATGCCTGAGTCGGATTCGGGTgagatgcg > 2:427124/1‑136 (MQ=25)
gAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAGCTGCACGTACAAGGTAAGCAGCTCGAAGAGACTGGCAGGAGTGCGAGCATTCGAACCGCGGACTGCCGGAGTCAGAATCCTGAGCCCTaac > 2:125293/1‑141 (MQ=12)
cGGAATTAGAATCCGGTGCCTTACCGCTTGGTGATACCCCAACTGGGTTCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg < 1:170936/143‑1 (MQ=255)
|
CCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGG > NC_000913/696338‑696606
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
