Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A23 F107 I0 R1
|
44 |
28.8 |
1140136 |
90.5% |
1031823 |
142.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
696,470 |
C→T |
100% |
noncoding (35/75 nt) |
glnX ← |
tRNA‑Gln |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 696,470 | 0 | C | T | 95.8%
| 48.8
/ ‑5.8
| 19 | noncoding (35/75 nt) | glnX | tRNA‑Gln |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (10/8); minor base A (0/1); total (10/9) |
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGT > NC_000913/696332‑696592
|
tCGCATCCGCCATTGAAGGATAGGACGTGTCAACAGCGCATTCGACATTGAATGAACTCAGAAAAGCAAAAAGCTCGCCTAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGaa < 2:378355/143‑1 (MQ=38)
tCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAACAGCTCGCCGAATCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAAGTAGaa > 2:552828/1‑143 (MQ=255)
tCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGaa < 1:450686/143‑1 (MQ=255)
tGAGGGATAAGACGTGTTACCAGCGCATTCGACAGTGAATGAACGCAGAAAAGCCAAAAGCTCGCCGAAGCGCGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTAc < 1:234111/143‑1 (MQ=25)
tCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTGTAATGTGGCCGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAcccc > 2:35912/1‑143 (MQ=255)
cATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCATCCCGCTTGGCGATACCCCACCTgggcg > 1:319791/1‑141 (MQ=255)
ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACaa > 1:192099/1‑143 (MQ=255)
aatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGt < 2:263029/143‑1 (MQ=255)
cAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCt < 1:164711/143‑1 (MQ=255)
aaaaGCAAAAAGCTCGCCGAAGCGAGCTTGTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGa < 2:385077/143‑1 (MQ=255)
gCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCGCAACTGTGTGTAGTTTTAGGGCAAGCGCCTTGAATAAATTGGCt > 1:506213/1‑143 (MQ=18)
gcGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATGCGAACCTCGGAATGCCGGAATAAGTATCCGGTGCCTTAAAGCTTGGCTATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTgg < 2:201241/142‑1 (MQ=25)
ccGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTATTGAATAAATTGGCTGGGGt > 1:266214/1‑143 (MQ=255)
cGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATGCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGGACTTACAAGGACAGCGTCTGGAATAAATTGGCTGTGGTa > 2:466930/1‑143 (MQ=21)
aaGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGGCCTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACg > 1:388659/1‑143 (MQ=255)
cGAGCTTGTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAgg < 2:46574/143‑1 (MQ=255)
gCTGTTTTGATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAtt < 2:303895/143‑1 (MQ=255)
gAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACAGCTTGGCGATACCCAAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTCGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATGAg > 1:522126/1‑143 (MQ=37)
gAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGAGTGCACTTACAAGGTACGCGTCTTGAATAAATTGGCTGGGGTACGAGGCTACGAACCTCGGAATGCCGGAATCAGAATCCGGt > 2:445042/1‑143 (MQ=37)
|
TCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGT > NC_000913/696332‑696592
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
