Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F51 I1 R1	25	46.9	1811498	90.3%	1635782	141.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	96.1%	139.8 / NA	51	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (24/25); total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGC  >  NC_000913/696330‑696607

cGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAg                                                                                                                                         <  2:845121/143‑1 (MQ=255)

      aTCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCg                                                                                                                                   <  2:91840/143‑1 (MQ=255)

        ccGACATGGAAGGGTAAGACTAGTTAACTGCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGt                                                                                                                                 <  2:673043/143‑1 (MQ=37)

         cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAGTCCGGTg                                                                                                                                >  1:131771/1‑143 (MQ=255)

         cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTg                                                                                                                                >  1:899368/1‑143 (MQ=255)

          gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGGCGATGCGAGCGTTTTTAAGGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGCATTAGAATCCGgcgc                                                                                                                               >  2:734523/1‑143 (MQ=38)

            cATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCt                                                                                                                             >  1:544023/1‑143 (MQ=255)

              gtGAAGGATAAGACGCGCCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTa                                                                                                                           <  1:740943/142‑1 (MQ=255)

               tGAAGGATACGACGCGTCAACATCGCATTCGACATTGCATGAACGCAAAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTAc                                                                                                                          <  2:216546/143‑1 (MQ=37)

               tGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTAc                                                                                                                          >  2:765012/1‑143 (MQ=255)

                 aaGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGGATCCGGTGCCTTACCg                                                                                                                        >  1:341212/1‑143 (MQ=255)

                 aaGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCg                                                                                                                                   <  2:652550/132‑1 (MQ=255)

                 aaGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCg                                                                                                                                   >  1:652550/1‑132 (MQ=255)

                         gACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAATAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGCATCAGAATCCGGTGCCTTACCGCTTGGCGa                                                                                                                >  1:862877/1‑143 (MQ=255)

                          aCGCGTCAACTTCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGATTCCGGTGCCTTACCGCTTGGCGAt                                                                                                               <  2:679425/143‑1 (MQ=255)

                           cGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATa                                                                                                              <  1:348477/143‑1 (MQ=255)

                            gtgtCCACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGACGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACATCGGAACGCCGGACTTAGACTCCGGCGCCGTACCGCTTGGCGATAc                                                                                                             >  2:620963/1‑143 (MQ=37)

                                 aaCATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaa                                                                                                        >  1:59640/1‑143 (MQ=255)

                                 aaCATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaa                                                                                                        >  1:344549/1‑143 (MQ=255)

                                       gCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGt                                                                                                  <  2:810641/143‑1 (MQ=255)

                                        cATTCGACATTGCATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTg                                                                                                 >  1:368816/1‑143 (MQ=255)

                                        cATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTg                                                                                                 >  1:522928/1‑143 (MQ=255)

                                         aTTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGc                                                                                                >  1:588440/1‑143 (MQ=255)

                                          tttgACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCa                                                                                               <  1:746772/140‑1 (MQ=255)

                                           tCGACATCGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTGTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAc                                                                                              <  1:592110/143‑1 (MQ=255)

                                              aCATTGAATGAACGCAGGAGATCAAAAAGCCCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTAGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGAGACCCCAACGGGGTGCACTTa                                                                                           >  2:230461/1‑143 (MQ=21)

                                                 ttGAATGAACGCAGAACAGCAATAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACaa                                                                                        <  2:468314/143‑1 (MQ=255)

                                                     atgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTCGACCACGGTGCCTTACCTCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTa                                                                                    <  1:172203/143‑1 (MQ=37)

                                                             atatacgCAAAAAGCTCGCCTAAGCGTGCTTTTTTAATTTGGCTGGTGTACGACGATTCGAACCTCGGCATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                            <  2:556837/137‑1 (MQ=21)

                                                             aGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                            <  1:530922/143‑1 (MQ=255)

                                                                 aaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCAGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGGACTTCCGAGGGAAGCGTCTAGAAt                                                                        >  2:534706/1‑143 (MQ=21)

                                                                    cAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACGAGCTCACCGCCTTGAAtcag                                                                     >  1:680445/1‑140 (MQ=21)

                                                                     aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAt                                                                    <  2:816476/143‑1 (MQ=255)

                                                                      aaaaGCTCGCCGAAGCGTGCTTTTTTAATGGGGCTGTGGTACGAGCATTCGAACCTCGGAATGCCGGAATTAGAACCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACTAGGTAAGCGTCTTGAATAAAtt                                                                   <  1:446779/143‑1 (MQ=25)

                                                                      aaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATaaact                                                                   >  1:609290/1‑141 (MQ=255)

                                                                        aggcgcgcgGAAGCGAGCTTTTTTAATGGGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTgg                                                                 <  2:735463/138‑1 (MQ=25)

                                                                            tCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTgg                                                             >  1:748815/1‑143 (MQ=255)

                                                                             cggcgtcgCGAGCTTTGTTAGTGGTGCTGGCGTAGCAGGTGTCGAACCTCGGAATGCCGGAATTAGAATTCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCTTCTTGAATAAAGTGGCTggg                                                            <  2:233241/136‑1 (MQ=18)

                                                                             cggcGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGACACGCGGAAGGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTggg                                                            <  1:855491/140‑1 (MQ=25)

                                                                                cGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTGCAAGGTCCGCGTCTTGACTATATTGGCTGGGGTa                                                         >  2:315930/1‑143 (MQ=21)

                                                                                 gAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGGACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGAGGTAc                                                        >  1:129162/1‑143 (MQ=255)

                                                                                   aGCGAGCTTTTTTAACGTGGCTGGGGTACTAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGa                                                      <  2:494151/143‑1 (MQ=255)

                                                                                               tAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTAAAAGGTAAGCGTCTGGAATAAATTGGCTGGGGTACGAGGATTCGAAcgt                                          >  1:225159/1‑141 (MQ=255)

                                                                                                aaTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTc                                         <  1:634796/143‑1 (MQ=255)

                                                                                                        tGGGGTACGAGGATGCGAACCTCGGAATGCCGGAATTAGAAGCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGcc                                 <  1:386679/143‑1 (MQ=255)

                                                                                                               cGAGGATTCGAACCTCGGAATGCCGGAATTCGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCa                          <  1:374684/143‑1 (MQ=255)

                                                                                                                gAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTAACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGGACAGAGGAATGCCGGAATCAg                         >  1:873998/1‑143 (MQ=25)

                                                                                                                 aGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGa                        <  1:671302/143‑1 (MQ=255)

                                                                                                                   gactCGACCCTCGGCATGCCGGAAGTAGAATCCGGTGCCTTACCGCTTGGCGATACGCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                      <  2:588440/140‑1 (MQ=38)

                                                                                                                         aaCCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACACGGTCCGCGCCTCGAATACATCTGCTGGGGTACGAGGCTACGAACCTCGGACCGCCGGAATCAGAATCCggag                >  2:228107/1‑141 (MQ=21)

                                                                                                                                       ggAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgtc  >  1:161755/1‑141 (MQ=255)

CGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGC  >  NC_000913/696330‑696607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A