Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A23 F67 I0 R1
|
35 |
27.8 |
1096780 |
91.1% |
999166 |
142.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
696,470 |
C→T |
100% |
noncoding (35/75 nt) |
glnX ← |
tRNA‑Gln |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 696,470 | 0 | C | T | 100.0%
| 49.2
/ ‑6.7
| 21 | noncoding (35/75 nt) | glnX | tRNA‑Gln |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (11/9); total (12/9) |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCG > NC_000913/696339‑696601
|
cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTg > 1:389061/1‑143 (MQ=255)
gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGc > 1:27534/1‑143 (MQ=255)
aaGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCg > 2:191775/1‑143 (MQ=255)
ggtAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCtt < 2:8574/141‑1 (MQ=255)
tAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg > 1:432135/1‑143 (MQ=255)
aaGACGCGTCAATATCTCATTCTTTATTGAATTAACTCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGc < 1:199508/143‑1 (MQ=21)
aTCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTg > 1:225439/1‑143 (MQ=255)
cactCGACATTGGATGAACGCAGAAAGGCGAAAAGTCCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGCGGCTTCGCACCTCGGAATGCGGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTg < 2:84417/140‑1 (MQ=21)
gACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGAACGAGGATGCGAACATCGGAATGCCGGACACAGACTCCAGTACCTAACCGCTTGGCGCTAACCCAACAGGATggaagt > 2:136032/1‑138 (MQ=255)
aTTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCCACTGGGTGCACTTACa > 2:318316/1‑143 (MQ=255)
aTTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCACTTACa > 2:458856/1‑143 (MQ=255)
gCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCGATTACAAGGTAAGCGTCTTGAATaa > 1:527648/1‑143 (MQ=255)
cAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATaaa < 2:432135/143‑1 (MQ=255)
cagcctgtttAATGTGGCTGGGGTAGGAGGATTCGAGCCTCGGCATTCCGGAATTAGAATCCGGTGCCTTAGCGCTTGGCGATACCCGAACTGGGTGCACTTACAAGGTAAGTGTCTTGAATAAATTGGCTGGGGTACGAGGa < 2:34251/136‑1 (MQ=255)
tgtttttttAATGTTGTTGTGGTGCGACGATTTGATCTTTGGTTTGGCGAACTTAGGATCCGATGACTTTCGGCTTGGCGTTTCCCTAACTGGGTGTCCTTTCTCGTTAAGCCTCTTGAATATATTGGCTGGGGTACGAGGAt < 2:267097/140‑1 (MQ=255)
aaTGTGGCTGGGGTACGAGTATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTc < 2:335681/143‑1 (MQ=255)
ggggTACGGGGCTTCGAACCACGGAATGCCGGAATTAGAACCCGGTGCCTTACCGCGTGGCGAACCTCCAACTACGTCCTACCACGGCAATATCGTCTTGAATAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCg < 2:115191/143‑1 (MQ=16)
ggATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa < 1:419456/143‑1 (MQ=255)
ggATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGATGCATTTGCATGGTCCGCGTCTTGAAGAAACTGGCAGGGGTACGAGGCTACGACCCGCGGAGAGCCGGAATCacca > 2:307387/1‑140 (MQ=17)
gAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGAGTGCAATTACAAAGGAGGCAGCTCGAATATCTTGGATGCGGTGCGAGGCTTCGAACCGCGGAAGGCCAGAACCAGAATccaga > 2:322739/1‑140 (MQ=12)
aaTGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGATGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCAGGAGTACGAGGAGACGAACAGCGGAATGGCGGAAACAGCATCCGGAGCCCAACCg > 2:50247/1‑143 (MQ=21)
|
CGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCG > NC_000913/696339‑696601
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
