breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 124,241 C→T 100% Q409* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
RA 674,768 G→T 100% R6S (CGC→AGC)  leuS ← leucine‑‑tRNA ligase
RA 696,470 C→T 100% noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 754,446 A→G 100% L8P (CTC→CCC)  gltA ← citrate synthase
RA 760,485 G→A 100% G594S (GGC→AGC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 798,039 C→T 13.9% L131L (CTG→CTA ybhA ← pyridoxal phosphate/fructose‑1,6‑bisphosphate phosphatase
RA 1,196,220 C→T 44.5% H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T 44.9% T370T (ACC→ACT icd → isocitrate dehydrogenase
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp 100% insH21 insH21
RA 1,440,791 C→T 40.9% intergenic (‑7/‑267) ydbK ← / → ydbJ putative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ
RA 1,445,616 G→C 7.8% A856P (GCA→CCA)  ydbH → PF11739 family protein YdbH
RA 1,498,892 A→T 8.2% intergenic (+17/‑46) ydcH → / → rimL protein YdcH/ribosomal‑protein‑L12‑serine acetyltransferase
RA 1,498,894 C→G 8.4% intergenic (+19/‑44) ydcH → / → rimL protein YdcH/ribosomal‑protein‑L12‑serine acetyltransferase
RA 1,498,896 A→T 8.2% intergenic (+21/‑42) ydcH → / → rimL protein YdcH/ribosomal‑protein‑L12‑serine acetyltransferase
JC JC 1,572,474 IS5 (+) +4 bp 100% coding (2726‑2729/2796 nt) pqqL ← putative zinc peptidase
RA 1,576,033 T→C 9.8% V529V (GTA→GTG yddB ← putative TonB‑dependent receptor
RA 1,708,718 G→A 25.2% A600A (GCG→GCA rsxC → SoxR [2Fe‑2S] reducing system protein RsxC
RA 1,795,229 A→T 18.6% intergenic (‑77/+24) btuC ← / ← ihfA vitamin B12 ABC transporter membrane subunit/integration host factor subunit alpha
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,361 Δ2 bp 100% pseudogene (917‑918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,263,834 G→A 27.7% intergenic (‑339/‑29) fruB ← / → setB fructose‑specific PTS multiphosphoryl transfer protein FruB/sugar exporter SetB
RA 2,368,101 T→C 100% A21A (GCT→GCC arnA → fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
RA 2,661,821 C→G 100% C104S (TGC→TCC)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 3,041,634 A→C 100% T108P (ACC→CCC)  ygfZ → folate‑binding protein
RA 3,560,455:1 +G 100% pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,657,599 T→A 10.6% intergenic (+32/+387) hdeD → / ← arrS acid‑resistance membrane protein/small regulatory RNA ArrS
MC JC 3,815,859 Δ82 bp 100% [rph] [rph]
MC JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,600,614 T→C 12.6% N121S (AAC→AGC)  dnaC ← DNA replication protein DnaC

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 315852NA (NA)13 (0.480) 7/256 NT NA noncoding (624/1255 nt) IS3 repeat region
?NC_000913 = 315875 NA (NA)noncoding (647/1255 nt) IS3 repeat region
* ? NC_000913 = 316201NA (NA)5 (0.190) 4/246 NT NA noncoding (973/1255 nt) IS3 repeat region
?NC_000913 = 316220 NA (NA)noncoding (992/1255 nt) IS3 repeat region
* ? NC_000913 566777 =NA (NA)1585 (57.370) 244/262 NT 97.9% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 = 572471 35 (1.260)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
* ? NC_000913 = 56684110 (0.360)14 (0.680)
+34 bp
11/196 NT 45.7% noncoding (65/1258 nt) IS3 repeat region
?NC_000913 = 572471 35 (1.260)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
* ? NC_000913 1207790 =0 (0.000)9 (0.370) 8/232 NT 90.0% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 2 (0.080)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12078050 (0.000)8 (0.330) 8/232 NT 88.9% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 2 (0.080)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 1497378 =32 (1.150)4 (0.150) 3/250 NT 10.5% coding (523/1656 nt) opgD glucan biosynthesis protein D
?NC_000913 1497422 = 38 (1.440)coding (567/1656 nt) opgD glucan biosynthesis protein D
* ? NC_000913 = 249311927 (0.970)4 (0.150) 4/248 NT 13.0% coding (136/1251 nt) frc formyl‑CoA transferase
?NC_000913 = 2493124 28 (1.070)coding (131/1251 nt) frc formyl‑CoA transferase
* ? NC_000913 2729955 =NA (NA)3 (0.110) 3/252 NT NA noncoding (1203/1542 nt) rrsG 16S ribosomal RNA
?NC_000913 2729972 = NA (NA)noncoding (1186/1542 nt) rrsG 16S ribosomal RNA
* ? NC_000913 2942789 =31 (1.110)3 (0.110) 3/250 NT 10.3% noncoding (94/205 nt) gcvB small regulatory RNA GcvB
?NC_000913 2942808 = 23 (0.870)noncoding (113/205 nt) gcvB small regulatory RNA GcvB
* ? NC_000913 = 41714452 (0.070)4 (0.150) 4/258 NT 67.2% noncoding (2805/2904 nt) rrlB 23S ribosomal RNA
?NC_000913 = 4212832 NA (NA)noncoding (2790/2904 nt) rrlE 23S ribosomal RNA