Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F20 I1 R1	20	59.7	2309458	89.5%	2066964	142.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	97.9%	127.9 / NA	48	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (18/29); minor base G (1/0); total (19/29)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTA  >  NC_000913/696332‑696598

tCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGACTTAGaa                                                                                                                              >  2:576257/1‑143 (MQ=255)

   cATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGCATGAACGCAGAAAAGCAAAAAGCGCGCCGAAGCGAGCATTTGTAATGTGGCTGCGGTACGAGGCTTCCAACCCCGGCCTCGCGGAATGAGAAAcc                                                                                                                           >  2:794997/1‑143 (MQ=255)

       cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGTTATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTg                                                                                                                       <  1:520835/143‑1 (MQ=255)

       cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAACTAGACGCCGGTg                                                                                                                       >  2:966968/1‑143 (MQ=255)

        gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGc                                                                                                                      >  1:892985/1‑143 (MQ=255)

         aCATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGcc                                                                                                                     <  1:1045782/143‑1 (MQ=255)

            ttGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTa                                                                                                                  >  1:719000/1‑143 (MQ=255)

             tGAAGGATAAGACTTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGGCGACGAGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTAc                                                                                                                 <  2:1062546/143‑1 (MQ=37)

             tGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTGTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTAc                                                                                                                 <  1:694829/143‑1 (MQ=255)

               aaGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCg                                                                                                               >  1:103158/1‑143 (MQ=255)

                  gATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCtt                                                                                                            >  1:1054014/1‑143 (MQ=255)

                    tAAGACGGGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGGTTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg                                                                                                          <  2:113351/143‑1 (MQ=255)

                         cgggtCAACATCGCATTCGAGATTGAATGAACGCAGAAGAGCAAAAGGCGCGCCGAAGCGAGCGTTTGTAGGGGGGCTGGGGGACGAGGATGCGAACGTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATa                                                                                                     <  1:700484/140‑1 (MQ=21)

                         cGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATa                                                                                                     <  2:196041/143‑1 (MQ=255)

                         cGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATa                                                                                                     <  2:150565/143‑1 (MQ=255)

                         cGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATa                                                                                                     <  1:757436/143‑1 (MQ=255)

                           tgtCACCATCGCATTCGACATTGCATGAACGCAGAAAAGCAAAACGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGTAATGCCGCAATTAGAATCCGGTGCCTTACCGCTTGGCGATAcc                                                                                                   <  2:705780/143‑1 (MQ=25)

                               aaCATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaa                                                                                               >  2:584303/1‑143 (MQ=255)

                                     gCATTCGACAGTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGt                                                                                         <  2:891384/143‑1 (MQ=255)

                                        ttCGACATCGAACGTCCGCCGAAAAGCAAGCAGCTCGCCGGAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCa                                                                                      <  2:742529/143‑1 (MQ=18)

                                        atccaCATTGTATGAACGCAGAAAAGCAAAAAGCTCGCCTCAGCGAGCTTTTTTAAGGTGGCGGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCa                                                                                      <  2:625602/139‑1 (MQ=21)

                                         tCGACATTGAATGAACGCAGAGAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAc                                                                                     <  1:656245/143‑1 (MQ=255)

                                          cGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                                                                                    >  1:576822/1‑143 (MQ=255)

                                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCAGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCACTTACat                                                                               >  2:126701/1‑142 (MQ=255)

                                                     gaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGCTGCACTTCCAAGGTAAg                                                                         >  1:1064192/1‑143 (MQ=255)

                                                           aGAAAATCAAAAAGCTCTCCGAAGCGAGCTTTTTTAATGTGTCTGGTGTACGAGGTTTCGTACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                   <  1:1122938/143‑1 (MQ=25)

                                                           aGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCAGGGTATTCCGTAGTTAGATTCCTGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                   <  1:1147882/143‑1 (MQ=37)

                                                             aaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGa                                                                 <  1:1047176/143‑1 (MQ=255)

                                                               aaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAGGCACACTTACAAGGTAAGCGTCTTGAAt                                                               >  1:363177/1‑143 (MQ=21)

                                                                  cAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATaaa                                                            <  1:911564/143‑1 (MQ=255)

                                                                   aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGACTCCGGTGCCTTACCGCTTGGCGATACCCCACCTGGGCGCACTTAGCCGGCACGCGTCTAGACTAAAt                                                           >  2:774571/1‑143 (MQ=18)

                                                                    aaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAtt                                                          >  1:819660/1‑143 (MQ=255)

                                                                     aaaGCGCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTg                                                         <  1:544122/143‑1 (MQ=255)

                                                                       aGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGc                                                       <  2:892985/143‑1 (MQ=255)

                                                                        ccgcGCCGAAGCGAGCTTTGTTACTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCt                                                      <  1:299232/140‑1 (MQ=255)

                                                                              cGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTACGCGTCTTGAATACATTGGCTGGGGTa                                                >  1:874324/1‑143 (MQ=255)

                                                                                  gCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGCCTTGACTAAATTGGCTGGGGTACGAg                                            >  1:482604/1‑143 (MQ=255)

                                                                                     aGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAt                                         <  1:436749/143‑1 (MQ=255)

                                                                                         tatttAATGTGGCTGGGGTACGAGGACACGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGa                                     <  1:854194/141‑1 (MQ=255)

                                                                                          ttttAATGTGGCTGGGCTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGNATAAATTGGCTGGGGTACGAGGATTCGaa                                    <  1:1113699/143‑1 (MQ=255)

                                                                                                       ggcgcaCGAGAACTCGACCCTCGGAACGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGCTACCCCAACTGGGTGCACTTACAAGTTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCg                       <  1:596614/138‑1 (MQ=37)

                                                                                                        gggTACGAGGATTTGAACCTCAGAATGGCGGAATTAGAATCCGGGGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCgg                      <  1:765308/143‑1 (MQ=37)

                                                                                                          gTACGAGGATTCGACACTCGTAATGCCGGGATTAGAATCCGGGGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGAGTACGAGGATTCGAACCTCGGAATGCCGGaa                    <  2:518228/143‑1 (MQ=37)

                                                                                                              gAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTCAGCGCCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATcgg                >  1:782711/1‑141 (MQ=255)

                                                                                                                 gATTCGAACCTCGGAATGCCGGAATTAGAATCCCGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTCCGCGCCTTGAAGCAACCGGCTGGGGCACGAGGCTTCGCACCGCCGAATGCCGGAGTCAGAAt             >  1:188315/1‑143 (MQ=18)

                                                                                                                        aCCTCGGAATGCTGGTATTAGAATTCGGTGCCTTACTGTTTGGCGATATTCCAATTGTTTGCATTTAAAAGGTAAGCGACGTGAAAAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc      <  1:1057088/143‑1 (MQ=18)

                                                                                                                          cTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTACCGAAACCCCAACTGGATGCACTTAGAAAGCAAGCGTATGGAATAAAATGGCTGGAGTACGAGGAGCGGAACCACGGAAGGCCGGAATGAGAATACGGTgggt    >  2:291382/1‑140 (MQ=16)

                                                                                                                            gggAATGCCTGAATTATAATTCGGTGCCTGACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTa  <  1:775640/142‑1 (MQ=37)

TCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTA  >  NC_000913/696332‑696598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A