Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F22 I1 R1	19	64.2	2479524	91.1%	2258846	141.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	97.9%	143.7 / ‑6.8	48	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (26/21); minor base A (0/1); total (26/22)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTT  >  NC_000913/696331‑696597

gTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGa                                                                                                                              >  2:855751/1‑143 (MQ=255)

   gaaTCGGACGTTGAAGGATAAGGCGTGGCAACATCGGATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGGGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATc                                                                                                                           <  1:552611/141‑1 (MQ=37)

        ccaCATTGAAGGATAAGACGTGGCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTg                                                                                                                      <  1:759276/141‑1 (MQ=255)

         gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGc                                                                                                                     >  1:1030373/1‑143 (MQ=255)

                   gATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCtt                                                                                                           <  1:68710/143‑1 (MQ=255)

                     tAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGCAATGCCGGAATTAGAATCCGGTGCCTTACAACTTgg                                                                                                         >  1:1092851/1‑143 (MQ=255)

                       aGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCg                                                                                                       <  2:135395/143‑1 (MQ=255)

                         aCGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGAt                                                                                                     >  1:716922/1‑143 (MQ=255)

                         aCGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGAt                                                                                                     >  2:503216/1‑143 (MQ=255)

                             gtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTGTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGGATTCGAATCCGGGGCGTTGCCGCCTGGCGAGAccc                                                                                                 >  1:461682/1‑143 (MQ=37)

                              tCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAcccc                                                                                                >  1:819041/1‑143 (MQ=255)

                              tCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAcccc                                                                                                >  2:49216/1‑143 (MQ=255)

                                    tCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGCTACGATGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                                                                                          <  1:1106216/143‑1 (MQ=255)

                                       cATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGACTTAGACTCCGGTGCCTTACCGCTTGGCGATACCCCAACTGAGTg                                                                                       >  2:858729/1‑143 (MQ=255)

                                         ttCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCa                                                                                     <  2:922640/143‑1 (MQ=255)

                                          tCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCAc                                                                                    >  2:751458/1‑143 (MQ=255)

                                              cATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGGCTTACCGCTTGGCGATACCCCAAGTGGGGGCACttgc                                                                                >  1:214114/1‑141 (MQ=255)

                                               aTTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCACACAGGCTGCACTTACa                                                                               >  2:236670/1‑143 (MQ=25)

                                                ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACaa                                                                              >  1:813488/1‑143 (MQ=255)

                                                tggaatgagCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACaa                                                                              <  1:810032/141‑1 (MQ=255)

                                                   agtgaaCGCAGAAGAGGAAGACGCTCGCCGAAGCGGGCTGTTGTACTGTGGCCACAGTACGAGGACCCGAACCGCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGt                                                                           <  2:259251/141‑1 (MQ=18)

                                                   aatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGCTGGAGGTACAAGGt                                                                           >  2:581335/1‑143 (MQ=21)

                                                     tgaaCGCAGAAAGGCAAACAGCCCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTaa                                                                         <  2:971005/143‑1 (MQ=255)

                                                          gCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTc                                                                    <  2:469259/143‑1 (MQ=255)

                                                            agagAAGGAAAAAGGCCGCCGAAGCGAGCTTTGGTAATGTGGCTGGGGTACGAGGGTTCGAACCTCGGAATGCCGGAATTAGGATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                  <  2:406908/143‑1 (MQ=21)

                                                            aGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                  <  1:819231/143‑1 (MQ=255)

                                                            aGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGGGCACTCACACGGTAAGCGCCtt                                                                  >  1:721297/1‑143 (MQ=21)

                                                            aGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACACCAACAAGTTGGACTTACAAGGTAcgcgcatt                                                                  >  2:1166258/1‑139 (MQ=18)

                                                                  gCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTAGGTGCACTTACAAGGTAAGCGCCTTGAATaa                                                            >  2:563738/1‑143 (MQ=255)

                                                                   cAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATaaa                                                           <  1:49216/143‑1 (MQ=255)

                                                                      ggaGCTCGCCGAAGCGAGCTTTTTGAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGACATAAAAATCTGGAGTTATCAATGGTGTCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTg                                                        <  2:878056/141‑1 (MQ=21)

                                                                            cGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTggg                                                  <  1:855751/143‑1 (MQ=255)

                                                                            cGCCGAAGCGAGCTTGTGTAATGAGGCTGGGGTACGAGGATCCGAACCTCGGAATGCCGGAATTAGACTCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTggg                                                  <  1:1198006/143‑1 (MQ=25)

                                                                             gCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCCCTTACAAGGTAAGCGTCTTGAATAAATTGGCTgggg                                                 >  1:492834/1‑143 (MQ=255)

                                                                                     gAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGa                                         >  1:884339/1‑143 (MQ=255)

                                                                                       gCTTTTTTAATTTGGCTGGGGTACGAGGATCCGAACCTCGTAATGCCGGAATTAGACTCCGGTGCCTTACCGCTTGGTGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAtt                                       <  2:451235/143‑1 (MQ=25)

                                                                                        cTTTTTGAATGTGGGTGGGGTAGGAGGAGTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTCCCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTc                                      <  1:984655/143‑1 (MQ=25)

                                                                                         ttttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAACCGGCTGGGGTACGAGGATTCg                                     >  1:457558/1‑143 (MQ=255)

                                                                                         ttgtttAATGTGGCTGGGGTACGAGCCTTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTCAATAAATTGGCTGGGGTACGAGGATTCg                                     <  2:1013230/140‑1 (MQ=25)

                                                                                          tttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAAGTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTAACAAGGGAAGCGGCGTGACTAAACTTGCAGGGGGACGAGGATTCGa                                    >  1:558367/1‑143 (MQ=18)

                                                                                           ttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGaa                                   >  1:613998/1‑143 (MQ=255)

                                                                                                 tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTGCAAGGTAAGCGGCTTGACTAAATTGGCTGGGGTACGTGGCTTCGaccggcgg                             >  1:1128464/1‑136 (MQ=21)

                                                                                                   tgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGaa                           <  1:727706/143‑1 (MQ=255)

                                                                                                     gCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGCATg                         >  1:135789/1‑143 (MQ=255)

                                                                                                              cGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTACGCGTCTTGAATAAATTGGCTGGGGTACGCGGATTCGAACCTCGGAATGCCGGAATCa                >  1:141722/1‑143 (MQ=255)

                                                                                                                       gAACCTCGGAATGCCGGAATTAGAAGCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt       <  1:1045469/143‑1 (MQ=255)

                                                                                                                       gAACCTCGGAATGCCGGAAGTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt       <  1:755148/143‑1 (MQ=255)

                                                                                                                            tCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGTGTACGAGGATTCGAACCTCGGAATGGCGGAATCAGAATCGGGTGACtt  >  1:472381/1‑143 (MQ=37)

GTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTT  >  NC_000913/696331‑696597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A