Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A29 F18 I1 R1	19	60.0	2279412	90.4%	2060588	142.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	95.4%	172.5 / NA	66	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (26/36); minor base G (1/2); total (28/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGC  >  NC_000913/696334‑696607

gCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATc                                                                                                                                     <  2:237281/143‑1 (MQ=255)

  atgcGACATTGAAGGATAAGACGTGGCAACAGCGAATCCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCg                                                                                                                                   <  1:404600/140‑1 (MQ=38)

  aTCCGACATTGAAGGATAAGCCGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCg                                                                                                                                   <  1:322687/143‑1 (MQ=255)

   tCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCgg                                                                                                                                  >  1:856193/1‑143 (MQ=255)

     cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTg                                                                                                                                >  1:486439/1‑143 (MQ=255)

      gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGCATGCCGGAATTAGAATCCGCTGc                                                                                                                               >  1:505347/1‑143 (MQ=255)

      gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATGAGAATCCGGTGc                                                                                                                               >  2:823680/1‑143 (MQ=255)

         aTTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCtt                                                                                                                            >  1:664821/1‑143 (MQ=255)

            gAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTAcc                                                                                                                         <  1:525613/143‑1 (MQ=255)

            gAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTAcc                                                                                                                         >  1:166810/1‑143 (MQ=255)

                  tAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg                                                                                                                   <  2:523746/143‑1 (MQ=255)

                  tAAGACGTGCCAACATCGCATTCGACATTGAAAGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTTGAACCTCGGAATGCCGGAATTAGAAGCCGGTGCCTTACCGCTTgg                                                                                                                   <  1:639151/143‑1 (MQ=37)

                  gaggtggtgtCAAGATGGCGTGCGTCATGGCATGCCCGCCGAAAAGCAAAGAGCTCGCCGAAGCGAGCGTTTTTAAGGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg                                                                                                                   <  2:355253/137‑1 (MQ=18)

                   gaGACGTGGCAAGATCGGTTTCTATATTGAATTAACGCAGAAAACCAAAAAGCTCGCCGAAGAGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGTAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGc                                                                                                                  <  2:257808/142‑1 (MQ=21)

                     gACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCATGGCGa                                                                                                                >  2:160600/1‑143 (MQ=255)

                         tgtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAcc                                                                                                            <  1:197531/143‑1 (MQ=255)

                             aaCATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGGATCCGGTGCCTTACCGCGTGGCGATACCCCac                                                                                                        >  2:844021/1‑142 (MQ=255)

                                 tCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTCGAGTCCGGTGCCTTACCGCTTGGCGATACGCCAACTgg                                                                                                    >  1:308759/1‑143 (MQ=255)

                                 tCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                                                                                                    <  2:322214/143‑1 (MQ=255)

                                     aTTCGACATTGAATGAACGCAGAACAGCCAACAGCTCGTGGGGGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGc                                                                                                <  1:472091/143‑1 (MQ=21)

                                     aTTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGc                                                                                                <  1:888421/143‑1 (MQ=255)

                                     aTTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAAATAGAATCCGGTGCCTTACCGCTTGGCGCAACCCCAACAGGgcgc                                                                                                >  2:875228/1‑143 (MQ=25)

                                         gACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTAAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGAGATACCCCAACTGGGTGCACtt                                                                                            >  2:532824/1‑143 (MQ=255)

                                            aTTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTGTTGTTGCTCAATAACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACa                                                                                         <  1:1128037/143‑1 (MQ=21)

                                            aTTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACGGGGTGCACTTaaa                                                                                         >  1:694252/1‑141 (MQ=255)

                                             ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACaa                                                                                        <  1:539172/143‑1 (MQ=255)

                                              tgaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAAGGTGGCTGGGGTACGATGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg                                                                                       <  2:916837/143‑1 (MQ=255)

                                               gaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAgg                                                                                      >  1:356587/1‑143 (MQ=255)

                                                 atgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTa                                                                                    >  1:945416/1‑143 (MQ=255)

                                                 atgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAGGTGGACTTGCacgcgc                                                                                    >  2:141183/1‑138 (MQ=21)

                                                    acgggagaagaGCAAAAAGCGCGCCGAAGCGAGGTTTTTTTCTGTCGCTAAAGTACGTGGTCTCAAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGc                                                                                 <  1:1012553/138‑1 (MQ=18)

                                                    aaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGc                                                                                 >  1:25655/1‑143 (MQ=255)

                                                      cGCAGAGAGGCAAGAAGCTCGCCGAGGCGGGCTTTTTGAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAAGTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt                                                                               <  2:773244/143‑1 (MQ=21)

                                                      cGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt                                                                               >  1:112284/1‑143 (MQ=255)

                                                         aGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                            <  1:778831/143‑1 (MQ=255)

                                                          gAAATGTAAAAAGCTCGCGGAGGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTg                                                                           <  2:540350/143‑1 (MQ=21)

                                                          gAAAAGCAAAAAGCGAGGCGAAGCGAGCTGTTGTAAGGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTg                                                                           <  1:67213/143‑1 (MQ=21)

                                                           aaaaGCAAAAAGCTCGCCGAAGCGAGCGTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGa                                                                          <  1:245965/143‑1 (MQ=255)

                                                                  aaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAtt                                                                   >  1:700086/1‑143 (MQ=255)

                                                                   aaggCTCGCCGAGGCGGTCTTTTTGAATGTGGCTGGGGTACGAGGATTCGAACCTCGGGATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTg                                                                  <  2:256368/140‑1 (MQ=21)

                                                                         cGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACGTCGGAATGCCGGAATTAAAAACCAATGCGTTTGGCCAAAGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTggg                                                            <  1:477600/143‑1 (MQ=25)

                                                                          gCCGGATCGAGCTTTTTTAATGTGGTTGGTGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGTTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACTAGGTACGCGTCTTGAATAAATTGGCTtggg                                                           <  2:427496/143‑1 (MQ=21)

                                                                           ccGAAGCGAGCTTTTGTAATGTGGCGGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGt                                                          <  1:721343/143‑1 (MQ=255)

                                                                                  gAGCTTTTTTAATGTGGCGGGGGTACGAGGATTCGAACCTGGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGa                                                   <  2:987786/143‑1 (MQ=255)

                                                                                   aGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAt                                                  >  1:787006/1‑143 (MQ=255)

                                                                                         gttAGTGCGGCTGGGGCGCGAGGACGCGAACCTCGGACTGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGCGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAAc                                            <  2:1011166/142‑1 (MQ=25)

                                                                                          ttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTCCGAGGATTCGAAcc                                           >  1:845536/1‑143 (MQ=255)

                                                                                            aaTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGACTAAATTGGCTGGGGTACGAGGATTCGAACGTc                                         >  1:122755/1‑143 (MQ=255)

                                                                                            aaTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTc                                         <  1:530599/143‑1 (MQ=255)

                                                                                            aaTGTGGCTGGGGGAGGGGGATTTGAACCTCGGAATGCCGGAATTAGGATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTc                                         <  2:862420/143‑1 (MQ=37)

                                                                                             aTGTGGCTTGGGCACGAGGGTTCGAACGTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCg                                        <  2:277385/143‑1 (MQ=37)

                                                                                                tgGCTGGGGTACGAGAATGCGAACCGCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGaa                                     <  1:875228/143‑1 (MQ=255)

                                                                                                 ggCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCACTTACAAGGTAAGCGTCTCGACTAAATTGTCTGGGGTGGGGGGATACGAACGTCggcgt                                    >  1:486994/1‑140 (MQ=18)

                                                                                                     ggggTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCg                                >  1:563180/1‑143 (MQ=255)

                                                                                                        gTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGaa                             <  1:922154/143‑1 (MQ=255)

                                                                                                           ggaggagTCGAGCCTCGGAAGGCCGGAATGAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCa                          <  1:1044240/142‑1 (MQ=255)

                                                                                                           cgcggcttCGAACCTCGGAATGGCGGAATTAGTATCCGGCGCCTTCCCGATTGGCGATACCCCAACTGGGTGAAATTACGAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCa                          <  2:856193/137‑1 (MQ=37)

                                                                                                           cGAGGATTCGAACCTCGGAACGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCa                          <  1:59808/143‑1 (MQ=255)

                                                                                                            gAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGTCTTACCGCTTGGCGATACCCCAACTGGGTGCCCTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGCATCAg                         >  1:932536/1‑143 (MQ=255)

                                                                                                               gATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTACGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGACCCTCGGCATGCCGGAGTCCGAAt                      >  1:735704/1‑143 (MQ=37)

                                                                                                                   cGAACCGCGGAATGGCGGGACTAGACACCGGCGCATTACGGCTTGGCGGTAACCCAACAGGGCGCAATTTCGAGGTAGGCGGCGTGAGTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                  <  2:184472/143‑1 (MQ=12)

                                                                                                                        cTCGGAATGCCGGAATGAGAATCCGGCGCCTTGCCGCTTGGCGATACCCCAACTGGGTGCACGTACAAGGTAAGCGTCTTGAATAAACTGGCTGGGGTCCGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt             <  1:586032/143‑1 (MQ=37)

                                                                                                                                gCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTCCGCGTCTTGAATAAATTGGCTGCGGTACGAGGATTCGAACCTCGGAATGCCGGAATGAGAACCCGGTGCCTAACCGCtt     >  2:1009525/1‑143 (MQ=37)

                                                                                                                                  cGGAATTAGAATCCGTTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTTTTTAATTTGGTTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg   <  1:85421/143‑1 (MQ=21)

                                                                                                                                  cGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg   >  1:356747/1‑143 (MQ=255)

                                                                                                                                   ggAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACACGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGCGGATTCGAACCTCGGAATGCCGGAACCAGAATCCGGTGCCTTACCGCCTGGc  >  2:769575/1‑143 (MQ=37)

GCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGC  >  NC_000913/696334‑696607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A