Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F50 I1 R1	21	62.9	2410612	90.0%	2169550	142.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	96.2%	156.9 / NA	54	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (25/26); minor base G (0/2); total (25/29)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGG  >  NC_000913/696332‑696606

tCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGaa                                                                                                                                      <  1:1133756/143‑1 (MQ=255)

     tCCGACATTGAAGGGTAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTGTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGCAATTAGAATCCgg                                                                                                                                 <  2:540405/143‑1 (MQ=255)

        gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTGTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAAGTAGAATCCGGGGc                                                                                                                              >  2:948351/1‑143 (MQ=255)

               aaGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCg                                                                                                                       >  2:569586/1‑143 (MQ=255)

                 ggATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCt                                                                                                                     >  1:182755/1‑143 (MQ=255)

                     aaGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGc                                                                                                                 >  1:689583/1‑143 (MQ=255)

                     aaGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTGTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGc                                                                                                                 <  1:477789/143‑1 (MQ=255)

                              cAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACAGCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCa                                                                                                        >  1:1191466/1‑143 (MQ=255)

                               aaCATCGCATTCGAGAGTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaa                                                                                                       <  2:444881/143‑1 (MQ=255)

                                   tCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACtag                                                                                                   >  2:399701/1‑141 (MQ=255)

                                   tCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCACCTGGCCATACCCCCAcgcc                                                                                                   >  2:221616/1‑140 (MQ=25)

                                   tCGCATTCGACAGTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                                                                                                   <  1:511428/143‑1 (MQ=255)

                                    cGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTggg                                                                                                  <  1:569586/143‑1 (MQ=255)

                                     gCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGt                                                                                                 >  1:243678/1‑143 (MQ=255)

                                           gACCTTGAATGAACGCAGAAAAGAAAAAAGCGCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACtt                                                                                           <  1:1174334/143‑1 (MQ=255)

                                           gACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACtt                                                                                           >  1:1197680/1‑143 (MQ=255)

                                            aCATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAGGTggatttc                                                                                          >  2:453606/1‑137 (MQ=21)

                                               ttGAATGAACTCAGCAAAGCAAAAAGCTCGCCAAAGCGAGGTTTTTTAATGTGGCTGGGGTACGAGGACTCGAACCTAGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACaa                                                                                       <  1:545098/143‑1 (MQ=21)

                                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACaa                                                                                       <  1:210408/143‑1 (MQ=255)

                                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACaa                                                                                       >  2:369707/1‑143 (MQ=255)

                                                tgaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg                                                                                      <  1:369707/143‑1 (MQ=255)

                                                   atgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTa                                                                                   >  1:338667/1‑143 (MQ=255)

                                                    tgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATCCCCCACCTGGGTGCACTTACAAggcca                                                                                  >  2:27123/1‑140 (MQ=255)

                                                        cGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTCAGCGt                                                                              >  1:585533/1‑143 (MQ=255)

                                                           aGAAAAGCAAAGAGCTCGCCGAAGCGAGCGTTTTCAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                           <  1:492404/143‑1 (MQ=255)

                                                                    aaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTCAGCGTCTTGAATAAAtt                                                                  >  1:104560/1‑143 (MQ=255)

                                                                    aaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAtt                                                                  >  1:104556/1‑143 (MQ=255)

                                                                      aaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTgg                                                                <  1:200814/143‑1 (MQ=255)

                                                                       agcgtgCCGAAGCGAGCTTTTTTAAGGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGc                                                               <  2:688153/138‑1 (MQ=255)

                                                                        gCTTGCCGACGCGAGCGATTGTAATGTGGCTGGGGGACGAGGATTCGAACCTCGGAAGGCCGGAATTAGACTCCGGTGCCGTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCt                                                              <  2:90917/143‑1 (MQ=21)

                                                                          tCGCGGAAGCGGGCTTTTGTAATGTGGCGGGGGTACGAGGATTCGCACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTgg                                                            <  2:1038309/143‑1 (MQ=25)

                                                                           cGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTAAGTGCACTTACAAGGGAAGCGCCTAGACTAAACTGGCTggg                                                           >  2:432845/1‑143 (MQ=21)

                                                                           cGCCAAAGCGAGCTATTCTAATGTGACTGCGGTACGAGGATTCTAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTggg                                                           <  1:693715/143‑1 (MQ=25)

                                                                            gCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTgggg                                                          >  1:997102/1‑143 (MQ=255)

                                                                               gAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTAc                                                       <  1:401396/143‑1 (MQ=255)

                                                                                 ggCGAGCTTTTGTAAGGGGGCTGGGGTACGAGGATTCGAACCTCGGCATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGa                                                     <  2:107943/142‑1 (MQ=25)

                                                                                 ctcGACCTTTTTTAATGTGGTTGGGCTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGa                                                     <  2:819043/141‑1 (MQ=255)

                                                                                 aGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGa                                                     <  2:338667/143‑1 (MQ=255)

                                                                                        ttttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGCTTCg                                              >  1:23997/1‑143 (MQ=255)

                                                                                         tttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGa                                             <  1:467514/143‑1 (MQ=255)

                                                                                          ttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGaa                                            <  1:1135658/143‑1 (MQ=255)

                                                                                           tttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTAGAAAATTTAGCGTCTTGAATAGATTTGCGGGGGTACGAGGATTCGAAc                                           >  1:749042/1‑143 (MQ=18)

                                                                                                    gCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGGGCACTTACAAGGTAAGCGTCGTGAAGAAATTGGCTGGGGTACGAGGCTTCGAGCCTCTGAATg                                  >  1:195060/1‑143 (MQ=21)

                                                                                                     cTGGGGTAGGAGGATCCGAACCTCGGAATGGCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGc                                 <  1:1022546/143‑1 (MQ=255)

                                                                                                     cTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCCCTTACAAGGTAAGCGCCTTGAATAAACTGGCTGGGGTGCGAGGATTCGAACCTCGGAGTGc                                 >  1:533229/1‑143 (MQ=21)

                                                                                                       ggggTACGGGGGTTCGAGCCTCGGAATGCCGGAATGAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACGGGGTGCGCTTACGAGTTATTCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCg                               <  1:504257/143‑1 (MQ=21)

                                                                                                        gggTCCGAGGATGCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCgg                              <  1:522261/143‑1 (MQ=255)

                                                                                                         ggTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGa                             >  1:455352/1‑143 (MQ=255)

                                                                                                           gaCGAGGATGCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAAt                           <  1:669391/142‑1 (MQ=255)

                                                                                                             cGAGGATTGGACCCTCGGAATGCTGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTTCAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGGATCa                         <  2:1197680/143‑1 (MQ=37)

                                                                                                                ggATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATACATTGGCTGGGGTACGAGGATTCGAACCTCGGCATGCCGGTATCAGaa                      >  1:572886/1‑143 (MQ=255)

                                                                                                                             ggAATGTCGGAATTAGAATACGGTGCCTTACCCCTTGAAGATACCCCAACTAGTTGCACTTACAACGTAAGCGTCTTGTATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAc         <  2:771003/143‑1 (MQ=25)

                                                                                                                                acgCCCGAATGAGAAGCCGGTGCCTTACCGCTTGTCGATACCCCAACTGGATGCACTTACAAGGTAAGCGTCTTAAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc      <  1:570547/141‑1 (MQ=25)

                                                                                                                                    cGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAAACCGACGAGTCGCACTTACATGGTCCGCGCCTTGCAGAAACTGGCTGCGGTACGAGGCTACGAAGCTCTGCCAGCCGGAATCGGTATCCTGAGCCTGTCCGcgctg  >  2:856642/1‑139 (MQ=255)

TCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGG  >  NC_000913/696332‑696606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A