Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F21 I1 R1	22	41.7	1602136	90.4%	1448330	141.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	100.0%	67.0 / NA	27	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/13); total (14/13)

GTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCG  >  NC_000913/696331‑696608

ggcGCATCCGACATTGCAGGTGAAGACGTGTCAACATCGCATTGGACATTGAATGCACGCAGAAAAGCAACAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGa                                                                                                                                         <  2:657156/141‑1 (MQ=38)

   gCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGCGCTTTTTTACTGTGGCTGGGGTACGAGGATACGAACATCGAAATGACGGAATTAGAATc                                                                                                                                      >  1:8027/1‑143 (MQ=255)

      tCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCCAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGCGGTACGAGGATTCGAACCCCGGAATGCCGGAATTAGAATCCgg                                                                                                                                   >  2:538027/1‑143 (MQ=255)

       ccGACATTGAAGGATAAGACTTGTCATCATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGTGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGt                                                                                                                                  <  2:5355/143‑1 (MQ=255)

        cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCGCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTg                                                                                                                                 <  1:522446/143‑1 (MQ=255)

         gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACAGTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGCCTGCCGGAATTAGAATCCGGTGc                                                                                                                                >  1:223188/1‑143 (MQ=255)

            aTTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCtt                                                                                                                             >  1:168574/1‑143 (MQ=255)

                         aCGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGAt                                                                                                                >  2:619789/1‑143 (MQ=255)

                                    tCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                                                                                                     >  2:798412/1‑143 (MQ=255)

                                     cGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGGCTGGCGATGCACCACCTggg                                                                                                    >  2:466020/1‑143 (MQ=25)

                                       cATTCTACATTGAAGTAACGAAGAAAAGCAAAAATCCCGCCGAAGCGAGCTTTTTGAAGGAGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTg                                                                                                  <  2:281134/143‑1 (MQ=18)

                                             aCATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGCTTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTGCCGCTTGGCGGTACCCCAACTGGGTGCGCTTa                                                                                            >  1:235949/1‑143 (MQ=25)

                                               aTTGAATGAACGCAGAAAAGCAAAAAGCTCGCTGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCGGGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACa                                                                                          <  1:114092/143‑1 (MQ=255)

                                               aTTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGCTACCCCAACTAGGTGCACTTACa                                                                                          >  2:556262/1‑143 (MQ=255)

                                                                          gtCGCCGAAGTGAGCTTTGTTAATGTGGCTGTGGTATGAGGATTCGAACCTCGGAATGCGGGAAGTAGAATCCGGTGCCTTACCGCTTGGCGATAACCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTg                                                               <  2:517981/142‑1 (MQ=25)

                                                                                        cTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTc                                                 >  1:651241/1‑143 (MQ=255)

                                                                                           ttttAATGGGGCTGGGGTACGAGGATTGGGAGCTCGGAATGACGGAATTAGAGTGGGGTGCGTTAGGGTTTGGGGATAACGCAACTGGGTATACGTACGTGGTGGGGGTGTTGAATAAATTGGCTGGGGTACGAGGATTCGaa                                              <  2:530191/143‑1 (MQ=255)

                                                                                             ttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTTCACTTGCAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGGTTCGAAcc                                            >  1:128531/1‑143 (MQ=255)

                                                                                               aaTGTGGCTGGGGTAGGAGGATTCGAACCTCGCAATGCGGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTc                                          <  2:48495/143‑1 (MQ=255)

                                                                                                       tGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGcc                                  <  1:417307/143‑1 (MQ=255)

                                                                                                        gtggTACGAGGATTCGAAGCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCg                                 <  1:466020/141‑1 (MQ=255)

                                                                                                                aGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGa                         <  1:278187/143‑1 (MQ=255)

                                                                                                                       gAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGCTTCGAACCTCGGAATGCCGGAATCACAATCCGGt                  >  1:261657/1‑143 (MQ=255)

                                                                                                                       caaCCTCGGAACGCCGGAATTAGAATCCGGTGCCTTCCCGCTTAGCGATACCCCAACTGGGTGCACTTACAATCTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                  <  1:330750/142‑1 (MQ=37)

                                                                                                                                    ccGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCg                                                >  2:36586/1‑100 (MQ=255)

                                                                                                                                    ccGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCg                                                <  1:36586/100‑1 (MQ=255)

                                                                                                                                       gAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGGCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAGTCAGAATCCGGTGCCGTACCGCTTGGCg  >  1:756010/1‑143 (MQ=255)

GTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCG  >  NC_000913/696331‑696608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A