breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 125,387 C→T 100% Q791* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
RA 696,470 C→T 100% noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 710,595 G→T 100% P18T (CCT→ACT)  fur ← DNA‑binding transcriptional dual regulator Fur
RA 759,250 G→T 100% R182L (CGC→CTC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 940,751 T→C 10.1% intergenic (+31/‑208) serS → / → dmsA serine‑‑tRNA ligase/dimethyl sulfoxide reductase subunit A
RA 1,196,220 C→T 100% H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T 100% T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C 100% L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G 100% L375M (TTA→CTG icd → isocitrate dehydrogenase
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp 100% insH21 insH21
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,028,160 G→C 16.4% intergenic (‑143/+28) dgcQ ← / ← yodC putative diguanylate cyclase DgcQ/protein YodC
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,361 Δ2 bp 100% pseudogene (917‑918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,402,140 G→A 100% P569L (CCG→CTG)  nuoC ← NADH:quinone oxidoreductase subunit CD
RA 2,661,969 C→G 100% V55L (GTT→CTT)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 3,041,632 T→A 100% V107E (GTG→GAG)  ygfZ → folate‑binding protein
RA 3,560,455:1 +G 100% pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,637,645:1 +T 100% coding (4/1500 nt) pitA → metal phosphate:H(+) symporter PitA
RA 3,657,599 T→A 9.6% intergenic (+32/+387) hdeD → / ← arrS acid‑resistance membrane protein/small regulatory RNA ArrS
RA 3,657,601 G→C 10.0% intergenic (+34/+385) hdeD → / ← arrS acid‑resistance membrane protein/small regulatory RNA ArrS
RA 3,657,603 T→A 10.1% intergenic (+36/+383) hdeD → / ← arrS acid‑resistance membrane protein/small regulatory RNA ArrS
RA 3,719,704 C→T 8.3% A76V (GCC→GTC)  yiaG → putative DNA‑binding transcriptional regulator YiaG
MC JC 3,815,859 Δ82 bp 100% [rph] [rph]
JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,125,097 C→T 100% E572K (GAG→AAG)  priA ← primosome factor N'
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,296,060 C→T 17.2% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196276 1211449 15174 9 [8] [7] 9 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 213083 =39 (1.180)3 (0.090) 3/256 NT 7.6% coding (753/1299 nt) tilS tRNA(Ile)‑lysidine synthetase
?NC_000913 213120 = 35 (1.090)coding (790/1299 nt) tilS tRNA(Ile)‑lysidine synthetase
* ? NC_000913 225078 =NA (NA)4 (0.130) 4/250 NT NA noncoding (1308/1542 nt) rrsH 16S ribosomal RNA
?NC_000913 225100 = NA (NA)noncoding (1330/1542 nt) rrsH 16S ribosomal RNA
* ? NC_000913 392264 =NA (NA)6 (0.190) 4/256 NT NA noncoding (703/1258 nt) IS3 repeat region
?NC_000913 392281 = NA (NA)noncoding (686/1258 nt) IS3 repeat region
* ? NC_000913 566777 =NA (NA)2185 (66.390) 260/262 NT 98.7% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 = 572471 28 (0.840)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
* ? NC_000913 1409894 =27 (0.810)3 (0.090) 3/252 NT 11.0% coding (384/1374 nt) dbpA ATP‑dependent RNA helicase DbpA
?NC_000913 1409908 = 23 (0.730)coding (398/1374 nt) dbpA ATP‑dependent RNA helicase DbpA
* ? NC_000913 = 199954818 (0.540)4 (0.130) 4/248 NT 18.2% intergenic (‑68/+37) dcyD/tcyJ D‑cysteine desulfhydrase/cystine ABC transporter periplasmic binding protein
?NC_000913 = 1999563 19 (0.610)intergenic (‑83/+22) dcyD/tcyJ D‑cysteine desulfhydrase/cystine ABC transporter periplasmic binding protein
* ? NC_000913 3600976 =36 (1.090)3 (0.100) 3/240 NT 8.5% intergenic (‑193/+52) rpoH/ftsX RNA polymerase, sigma 32 (sigma H) factor/cell division protein FtsX
?NC_000913 3601013 = 32 (1.060)intergenic (‑230/+15) rpoH/ftsX RNA polymerase, sigma 32 (sigma H) factor/cell division protein FtsX
* ? NC_000913 = 4285380NA (NA)3 (0.160)
+56 bp
3/152 NT NA intergenic (‑167/+33) yjcH/acs conserved inner membrane protein YjcH/acetyl‑CoA synthetase (AMP‑forming)
?NC_000913 = 4460444 NA (NA)noncoding (29/36 nt) other REP338a
* ? NC_000913 4365125 =40 (1.210)3 (0.100) 3/250 NT 7.4% coding (232/339 nt) cutA copper binding protein CutA
?NC_000913 4365142 = 37 (1.180)coding (215/339 nt) cutA copper binding protein CutA