Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F2 I0 R1 193 6708.0 8620653 98.5% 8491343 136.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,928,345 T→G 9.4% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,928,3450TG 9.4% 354.9 / 2.5 187F277V (TTC→GTC) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (91/72);  new base G (20/4);  total (111/76)
Fisher's exact test for biased strand distribution p-value = 1.32e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 189 total aligned reads displayed.

TCCAACGAAACCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGAAAATTGTCGA  >  NZ_CP022686/3928207‑3928484
                                                                                                                                          |                                                                                                                                             
   aaCGAAACCTCCTTCATTCTCTACACCTCCGGCTCGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCtt                                                                                                                                           <  1:1050640/140‑1 (MQ=255)
    aCGAAACCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCGAAATGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACCCCATGTTTGGCGGCAAAGCGGGCGGCGTGTTCttt                                                                                                                                          >  4:651875/1‑140 (MQ=255)
     cGAAACCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAACGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCtttt                                                                                                                                         <  3:64845/140‑1 (MQ=255)
           ctcctGCATTCTCTACACCTCCGGCACGACCGGCAAACCTACAGGTGTGCAGCGTGATGTCGGCGGATAGGCGGTGGCGCTGGCGACCGCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCtt                                                                                                                                   <  4:118954/140‑1 (MQ=255)
           ctcctGCATTCTCTACACCTCCGGCACGACCGGCAAACATAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCGCGCTGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCtt                                                                                                                                   <  2:663459/140‑1 (MQ=255)
                     ctctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑Cgg                                                                                                                        <  4:900180/140‑1 (MQ=255)
                     ctctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGAGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑Cgg                                                                                                                        <  6:80962/140‑1 (MQ=255)
                      tctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGc                                                                                                                       >  2:1098106/1‑140 (MQ=255)
                       ctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCt                                                                                                                      <  5:731315/140‑1 (MQ=255)
                       ctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCt                                                                                                                      <  4:506354/140‑1 (MQ=255)
                       ctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGGGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCt                                                                                                                      <  1:439761/140‑1 (MQ=255)
                        tACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTGTGCTTCGGTAAT‑CGGCTg                                                                                                                     >  2:912265/1‑140 (MQ=255)
                        tACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CCGCTg                                                                                                                     >  2:391554/1‑140 (MQ=255)
                         acacCTCCGGCACGACCGGATATCCTAATGGTGTGCAGGGTGATGTCGGGGGTTTTGCGGTGGCGCTGGCGACCTCGATGGTCTCGATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑GGGCTgg                                                                                                                    <  3:246929/140‑1 (MQ=255)
                             cTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGggtgg                                                                                                                <  7:432843/140‑1 (MQ=255)
                               ccGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTATTTTGTTCTTAGGAGAT‑AGGCTGGGGGGTa                                                                                                              >  5:422625/1‑140 (MQ=255)
                                cGGCACGACTGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGCCCTCGATGGCCACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTATTTTGTGGTTCGGGTAT‑CGGCTGggtgggat                                                                                                             >  7:67349/1‑137 (MQ=255)
                                cGGCACGACCGGCAAACCTAATGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCATTTGTGCTTCGGAAAA‑CGGCTGGGTGGTAg                                                                                                             >  1:188428/1‑140 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGCCCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTat                                                                                                             >  4:988584/1‑139 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑Cgg                                                                                                                        <  4:842131/129‑1 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAg                                                                                                             >  8:930160/1‑140 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCGTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAg                                                                                                             >  4:540656/1‑140 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTGTTTTGTGGTTCGGCTAT‑CGGCGTGGTGGTAg                                                                                                             >  8:459119/1‑140 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGTGGTGCGGATAT‑CGGCTgggggggtg                                                                                                             >  4:72049/1‑137 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGGGTTCGATTGGGTTTCGGATAT‑CGGCTGgggggttg                                                                                                             >  3:308436/1‑138 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACAATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGATTCGGATAT‑AGGCTGGGTGGTAg                                                                                                             >  2:172784/1‑140 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATAGCCACCATTTTTGGCGGCAAAGCGGGCGGCGTGTACTTTTTTGCTTCGGATAT‑AGGCGAGGTGGTAg                                                                                                             >  1:689741/1‑140 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCGCGATGGACACCAATATTGGCGGCAACGCGGGCGGCGTGTGAATTTTGGGTTCGGATAT‑CTGCTGGGTGGGAg                                                                                                             >  2:281986/1‑140 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGAGACCTCGCTGGACACAATTTTTTGCTGCAAAGCGGTCGGCGTGTTCGGTTGTGCGTCGGCTAT‑CGGCGGGGTGGTAg                                                                                                             >  7:358951/1‑140 (MQ=255)
                                cGGCACGAACGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGCCCTCGATGGACACCATTTTTGGCGGCAAAGCTGGCGGCGTGTTCTTTTGTGCTTCGCAGAT‑CGGCTGGGTGGTAg                                                                                                             >  6:948975/1‑140 (MQ=255)
                                  gCACGAGCTGCAAACCTAAAGGTGTGCAGCGTGATTTCTGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGTCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGTTGGTAggg                                                                                                           <  8:100403/140‑1 (MQ=255)
                                  gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGGGTCGGGTAT‑AGGCTGGGTGGGAggg                                                                                                           >  7:184175/1‑140 (MQ=255)
                                  gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAggg                                                                                                           <  4:931057/140‑1 (MQ=255)
                                  gCACGACCGGCAAACATAAAGGTGTGCAGCGTGATGTCGTCTGATATGCGGTGGCGCTGGCGACCGCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGGAggg                                                                                                           <  7:909124/140‑1 (MQ=255)
                                    cggACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGGGGCGCTGGCGACCTCGATTGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCa                                                                                                         <  6:737841/138‑1 (MQ=255)
                                    ccGACCGGCAAACCTATAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGTCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCa                                                                                                         <  7:957667/139‑1 (MQ=255)
                                    aggACCGTCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGTATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTTGTAGGGCa                                                                                                         <  7:267885/138‑1 (MQ=255)
                                    aCGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCa                                                                                                         <  7:324525/140‑1 (MQ=255)
                                    aCGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCa                                                                                                         <  1:1029904/140‑1 (MQ=255)
                                    aCGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCa                                                                                                         <  4:158636/140‑1 (MQ=255)
                                     cGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCAt                                                                                                        <  1:966648/140‑1 (MQ=255)
                                       aCCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGCCCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCGTTTGTGCTTCGGCTAT‑AGGGTGGGTGGTAGGGCATTc                                                                                                      >  7:870908/1‑140 (MQ=255)
                                       aCCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGTCACCAGTTTTGGCGGCAATGCGGGCGGCGTGGTCTGATGTGCTTGGGAGAA‑CGGCTGGGTTGTTTGGCAttg                                                                                                      >  7:162068/1‑139 (MQ=255)
                                       aCCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGGGTCGGATAT‑CGGCTGGGTGGGTGGGCATTc                                                                                                      >  1:784962/1‑140 (MQ=255)
                                       aCCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCGTTTGTGCTTCGGATAT‑CGGGTGGGTGGTAGGGCagtg                                                                                                      >  4:212356/1‑137 (MQ=255)
                                       aCCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTGCTTTTGTGCTTCGGTTAT‑CGGCTGGGTGGGAGGGCATTc                                                                                                      >  3:616436/1‑140 (MQ=255)
                                       aCCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGTGCTTCGGATAT‑AGGGTGGGTGGTAGGGCATTc                                                                                                      >  8:866842/1‑140 (MQ=255)
                                          ggCAAACCTAAAGGTGTGCAGCGTGATGGCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGta                                                                                                   <  5:270711/140‑1 (MQ=255)
                                             aaaCCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTatat                                                                                                <  6:1025844/140‑1 (MQ=255)
                                                  tAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACCCCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGttt                                                                                          >  8:552541/1‑140 (MQ=255)
                                                    aaGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTTCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTAc                                                                                        >  2:419870/1‑140 (MQ=255)
                                                       gggtgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTCGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTAcgcg                                                                                     <  2:988511/138‑1 (MQ=255)
                                                         gtgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTTGCGGCAACGCGGGCGGCGTGTTGTTTTGTGCTTAGGATAT‑CGGCGGGGTGGTAGGGGATTCGGATAT‑CGTTTACGcgcc                                                                                   >  8:288430/1‑140 (MQ=255)
                                                         gtgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGGGTTCTTTTGTGCTTCGGATAT‑CGGGTGGGTGGTTGGGCATTCGTTTAT‑CGTTTACGcgcc                                                                                   >  8:144286/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGTCAAAGCGGGCGGCGGGTTATTTTTTTCGTAGGGTAT‑GTGCGTGGTGGTTGGGCTTTGTGATAT‑AGTTTAACcgccc                                                                                  >  5:177536/1‑139 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCTGCGTGTTGTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGGTTACGcgccg                                                                                  >  8:780689/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTGTGCTTCGGTTAT‑AGGCTGGGTGGTGTGGCAATCGGATAT‑CGGTTACGcgccc                                                                                  >  5:104811/1‑139 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTTTGCTTAGGATAT‑CGGGGGGGTGGGAGGGCATTAGTATAT‑CGTTTAAGccccg                                                                                  >  5:624546/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTTTGCTTAGGATAT‑AGGGTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGcgccg                                                                                  >  7:723114/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGcgccg                                                                                  >  2:296166/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTAGTATAT‑CGTTTACGcgccg                                                                                  >  8:43375/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCGGGGTGGTAGGGCATTCGTATAT‑CGTTTACGcgccg                                                                                  >  7:819011/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCCTTTGGGCTTCGGAAAT‑AGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTAAGcgacg                                                                                  >  6:575547/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTGTTTTGTGGTTCGGGTATCCGGGGGGGGGGTTGGG‑ATTGGTATTT‑CGGTTACCcgccc                                                                                  >  1:1011000/1‑139 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGGGTTGTTTTTGGCTTAGGGTAT‑CGGGTGGGGGGTTGGGCATTGGGTTAT‑GGTTTACGcgccg                                                                                  >  2:450301/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGAGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTGGTCGTCGGATAT‑AGGCTGGGTGGTAGGGCATTCGTATAT‑GGGTTACGcgccg                                                                                  >  3:907067/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATAAGCGGTGGCGCTGGCGACCTCGATGGACACCATTTATGGCGGCAAAGCGGGCGGCGTGGTAGTTTGTGCTTAGGCTAT‑CTGCTGGGTGGTAGGGCCATCTTATAT‑CTTGTACAcgccc                                                                                  >  2:296319/1‑139 (MQ=255)
                                                          tgCAGCGTGATGTAGGCGGATATGCGGTGGCGCTGGAGAACTCGATGGACACAATTTTAGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTAGGATAT‑CGACTGGGAGGTAGGGCATTCGTATAT‑CGTATAAGcgccg                                                                                  >  8:509768/1‑140 (MQ=255)
                                                           gCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTGTTTTTTGCTTAGGATAT‑AGGGTGGGTGGTTGGGCATTGGTATATAGGTTTAC‑cgccgc                                                                                 >  3:545028/1‑140 (MQ=255)
                                                           gCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGAGTGTTCTTTTTTGCTTGGGATAT‑CGGCTGGGTTGTTTGGCATTGGTTTAT‑CGTTTTCGcgcccc                                                                                 >  7:585129/1‑138 (MQ=255)
                                                                 tGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTATTTTGTGCTTCGGATAT‑CGGCTGGTTGGTAGGGGATTCGTATAT‑AGTTTACGCGCCGCTGCTgg                                                                           >  4:371356/1‑140 (MQ=255)
                                                                  gATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCGGGGTGGTAGGGCATTCGTATAT‑CGTTTACGCGCCGCTGCTGGc                                                                          >  2:242367/1‑140 (MQ=255)
                                                                    tGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGCGCCGCTgcgggcgg                                                                        >  6:540252/1‑140 (MQ=255)
                                                                      tCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGCGCCGCTGCTGGCgggg                                                                      <  1:632992/140‑1 (MQ=255)
                                                                      gcggcggATATGCGGGGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGCGCCGCTGCTGGCgggg                                                                      <  8:411284/139‑1 (MQ=255)
                                                                         gcggATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTTTGAGTCGGATAT‑CGGGTGGGTGGTAGGGGATTCGTATAT‑CGTTTTCGCGCCGCTGCTGGCGGGGATg                                                                   >  1:298740/1‑140 (MQ=255)
                                                                         gcggATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCtt                                                                                                                                           <  3:1047250/70‑1 (MQ=255)
                                                                         gcggATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCtt                                                                                                                                           >  4:1047249/1‑70 (MQ=255)
                                                                         gcggATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTGGGATAA‑CGGCTGGGTGGTGGGGCAGTCGGATAT‑AGGTTTCGCGCAGCTGAGGGCGGGGGTg                                                                   >  1:157607/1‑140 (MQ=255)
                                                                         gcggATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGCGCCACTGCTGGCGGGGATg                                                                   >  4:760713/1‑140 (MQ=255)
                                                                             atatGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGCGCCGCTGCTGGCGGGGATGGCGa                                                               <  6:378054/140‑1 (MQ=255)
                                                                             atatGCGGGGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGCGCCGCTGCTGGCGGGGATGGCGa                                                               <  3:704938/140‑1 (MQ=255)
                                                                               atGCGGTGGAGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCTTATAT‑CGTTTACGCGCCGCTGCTGGCGGGGATGGCGACt                                                             <  2:881997/140‑1 (MQ=255)
                                                                                   ggTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCg                                                         <  3:875191/140‑1 (MQ=255)
                                                                                    gTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGAGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGt                                                        <  1:291172/140‑1 (MQ=255)
                                                                                       gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTa                                                     <  8:823739/140‑1 (MQ=255)
                                                                                       gcgcGGGCGACCGCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGTGCTACGGATAT‑CGGCTGGGTGGTAGGGCATTCGGATAT‑CGTTTACGCGCCGCGGCTGGCGGGGATGGCGACTATCGTTTa                                                     <  7:832875/140‑1 (MQ=255)
                                                                                            ggCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAg                                                <  6:243297/140‑1 (MQ=255)
                                                                                            ggCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑AGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGaaa                                                >  2:1055030/1‑139 (MQ=255)
                                                                                            ggCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTGTTTTGTGCGTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTAAGCGCCGCGGCTGGCGGGGATGGCGACTATCGTTTACGAAg                                                >  1:514521/1‑140 (MQ=255)
                                                                                            ggCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCGTTTGTGCGTCCGATAT‑CGGCTGGGTGGTAGGGCATTCGGATAT‑AGTTTACGCGCCGCTGCTGGCGGGGTAGGCGCCTATCGTTTACGaac                                                >  4:660986/1‑139 (MQ=255)
                                                                                                 ccTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTg                                           <  8:819011/140‑1 (MQ=255)
                                                                                                  cTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGGTGGGTGGTAGGGCATTAGTATAT‑CGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGTTTGc                                          >  2:79271/1‑140 (MQ=255)
                                                                                                          acacCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTgg                                  <  8:585129/140‑1 (MQ=255)
                                                                                                          acacCATTTTTGGCGGCAAAGCGGGCGGCGTGTTATTTTGTGGTTCGGATAT‑AGGCTGGGTGGTAGGGCATTCGGATAT‑AGTTTACGCGCCGCGACTGGCGGGGCTGGCGCCTATAGTTTTCGAAGGAATGCCGACCTgg                                  >  4:336042/1‑140 (MQ=255)
                                                                                                              cATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCgg                              >  4:562310/1‑140 (MQ=255)
                                                                                                                    tGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTgcg                        <  4:409970/140‑1 (MQ=255)
                                                                                                                      gcggcAAAGCGGGCGGCGTGTTCTTTTGTGCTACGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTgcggc                      <  5:520793/140‑1 (MQ=255)
                                                                                                                      gcggcAAAGCGGGCGGCGTGTTATTTTGTGCTTCGGATAT‑AGGCAGGGTGGTAGGGCATTAGTATAT‑CGTTTACGCGCCGCTGCGGGCGGGGATGGGGTCTATCGTTTAAGAAGGATTGCCGACCTGGCCGGCCTgccgc                      >  6:221417/1‑140 (MQ=255)
                                                                                                                      gcggcAAAGCGGGCGGCGTGGTCTTTTTTGCTTAGGATAT‑GCGCGTGGTGGTAGGGGATAGGAATAT‑CGTAAACGCGCCGCTCCTTGCTCGGAGGGCGGGTATAGTTTACGAAGGTTTGCCGACCATGCCCGACTgccgc                      >  6:217238/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                             
TCCAACGAAACCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATAT‑CGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGAAAATTGTCGA  >  NZ_CP022686/3928207‑3928484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A