Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F35 I0 R1 304 9232.4 10623078 98.3% 10442485 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,928,345 T→G 9.3% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,928,3450TG 9.3% 440.6 / 14.5 230F277V (TTC→GTC) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (103/100);  new base G (24/2);  total (128/102)
Fisher's exact test for biased strand distribution p-value = 3.21e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

TCCAACGAAACCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGAAAATTGTC  >  NZ_CP022686/3928207‑3928482
                                                                                                                                          |                                                                                                                                           
       aaaCCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGT‑TTtgt                                                                                                                                    >  5:812558/1‑140 (MQ=255)
       aaaCCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCt‑ttt                                                                                                                                      >  3:203863/1‑138 (MQ=255)
        aaCCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTtgtg                                                                                                                                   <  1:1062597/140‑1 (MQ=255)
           ctcctGCATTCTCTACACCTCCGGCACGACCGGCAAACCTACAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCtt                                                                                                                                <  8:299126/140‑1 (MQ=255)
           ctcctGCATTCTCTACACCACCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGc                                                                                                                                  <  4:842302/138‑1 (MQ=255)
             cctGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCg                                                                                                                              >  2:893911/1‑140 (MQ=255)
                    tctcTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCTGGCGGCGTGTTCT‑TTTGTGCTTCGGatatag                                                                                                                       >  8:84087/1‑138 (MQ=255)
                     ctctACCCCTCCGGCTCGACCGTCAAACATCAAGGTGTGCAGCGAGAAGTCGGCTGATATGCGGTGGCTCTGGCGTCCTCGATGGACACCATTTTTGGCGGCTAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCTGATATCgt                                                                                                                      <  6:823907/140‑2 (MQ=255)
                     ctctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCGCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCg‑g                                                                                                                     <  2:449299/140‑1 (MQ=255)
                      tctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGTCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTAT‑ATTGTGCTTCGGATATAG‑Gc                                                                                                                    >  8:908627/1‑140 (MQ=255)
                      tctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCACGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTATGTTTGT‑CGGCGGATATCg‑gg                                                                                                                    >  6:270955/1‑139 (MQ=255)
                       ctACACCTCCGGCACGTCCTGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGGGGCGCGGGCGTCCTCGATGGACTCCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCg‑g                                                                                                                     <  3:704153/138‑1 (MQ=255)
                       ctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCg‑g                                                                                                                     <  3:268362/138‑1 (MQ=255)
                       ctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCt                                                                                                                   <  2:1176894/140‑1 (MQ=255)
                       ctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCt                                                                                                                   <  8:556892/140‑1 (MQ=255)
                       ctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCt                                                                                                                   <  2:776916/140‑1 (MQ=255)
                        tACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCg‑gg                                                                                                                    >  4:147530/1‑137 (MQ=255)
                        tACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTAT‑TTTGTGGTGCGGATATAG‑GCTg                                                                                                                  >  7:1225325/1‑140 (MQ=255)
                         acaaCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCt                                                                                                                   <  3:559577/138‑1 (MQ=255)
                          cacCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGGGGTCT‑TTTGTGCTTCGGATAAAG‑GCTg                                                                                                                  >  4:348981/1‑138 (MQ=255)
                             cTCCGGCACGACCGGCAAGCCTAAAGGTGTGCAGCGTGATGGCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGggtgg                                                                                                             <  7:338298/140‑1 (MQ=255)
                             cTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCTGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGTCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGggt                                                                                                               <  4:717237/138‑1 (MQ=255)
                             cTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCTAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGggtgg                                                                                                             <  7:807043/140‑1 (MQ=255)
                             cTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGggtgg                                                                                                             <  5:395362/140‑1 (MQ=255)
                             cTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGAGATCG‑CCTGggtgg                                                                                                             >  6:559276/1‑140 (MQ=255)
                             cTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCT‑TTTGTGCTTCGGATATCG‑GCTGggt                                                                                                               >  4:451371/1‑138 (MQ=255)
                             cTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGGGTTGG‑TTTGTGCGTGGGATATCG‑GCGgggggg                                                                                                             >  1:600494/1‑140 (MQ=255)
                               ccGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑CCGGggtggga                                                                                                           >  5:485651/1‑138 (MQ=255)
                               ccGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCG‑TTTGTGCTTCGGATATCG‑GCTGggtggtt                                                                                                           >  2:320509/1‑139 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCATAGCGGGCGGCGTGTTCT‑TTTTTGCTTGGGATATCG‑GCGGGGTGGTat                                                                                                          >  2:54472/1‑139 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGT‑TTTTTGGTTCGGATTTCG‑GGTGGGTGGTAg                                                                                                          >  5:1133542/1‑140 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAg                                                                                                          >  1:759711/1‑140 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAg                                                                                                          >  8:24349/1‑140 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTGGA‑TTTGTGGTTAGGATATCG‑GCTGGGTGGTat                                                                                                          >  7:993379/1‑139 (MQ=255)
                                  gCACTACAGGCAAACCTAAAGGTGTGCAGCGGGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATTGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAggg                                                                                                        <  1:123521/140‑1 (MQ=255)
                                  gCACGACCGGCAAACCTCAAGGTGTGCAGCGTGAGGTCGGCGGAGATGCGGGGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTTGTAg                                                                                                          <  3:815332/138‑1 (MQ=255)
                                  gCACGACCGGCAAACCTAACGGTGTGCCGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTGCT‑TTTGTGCTTCGGATAGCG‑GCTGGGTGGTAg                                                                                                          <  4:127625/138‑1 (MQ=255)
                                  gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATTGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAg                                                                                                          <  3:102287/138‑1 (MQ=255)
                                  gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAggg                                                                                                        <  7:100981/140‑1 (MQ=255)
                                  gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAggg                                                                                                        <  7:1101839/140‑1 (MQ=255)
                                  gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAggg                                                                                                        <  7:52305/140‑1 (MQ=255)
                                  gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAggg                                                                                                        <  7:461170/140‑1 (MQ=255)
                                  gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAg                                                                                                          >  4:1196087/1‑138 (MQ=255)
                                  gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTGT‑TTTGTGCTTCGGTTATCG‑GCTGGGTGGTAggg                                                                                                        >  1:1201240/1‑140 (MQ=255)
                                  ccACGACCGGCAAAGCTAAATGTGTTCAGCCTCATGTCGGCGGATATGCGGTTGCGCTGGCGACCTCGATGGACACCATTTTTGGCTGCTAAGCGGGAGGCGTGTTCT‑TTTGTGCTTAGGATATCG‑GCTGGGTGGTAggg                                                                                                        <  8:627375/139‑1 (MQ=255)
                                    aCGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAggg                                                                                                        <  3:971736/138‑1 (MQ=255)
                                    aCGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCa                                                                                                      <  2:1283627/140‑1 (MQ=255)
                                                ccTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTGGT‑TTTGTGCTTCGGATATCG‑GCGGGGTGGTTGGGCATTCGTATTTCGt                                                                                          >  7:93831/1‑140 (MQ=255)
                                                    aaGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAggg                                                                                                        <  8:646421/122‑1 (MQ=255)
                                                    aaGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTAc                                                                                      >  2:285680/1‑140 (MQ=255)
                                                         gtgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCt                                                                                                                   >  1:802679/1‑106 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGCCCTCGATGGCCACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTAT‑TTTGTGCGTCGGTTTTGG‑GCGGGGTGGGAGGGCATTAGGTTATAGTTTAAGcgccc                                                                                >  1:135917/1‑139 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGCCCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTGGGCTATCG‑GCTGGGTGGTAGGGCATTGGTATTTCGTTTTCGcgccg                                                                                >  5:732768/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGT‑TTTGTGGTTAGGATTTGG‑GCTGGGTGGTAGGGCATTAGTATATCGTTTACCcgccc                                                                                >  1:17780/1‑139 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATTTAG‑GGGGGGTGGGAGGGCATTGGGATATAGTTTACGcgccg                                                                                >  7:672667/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCCTTCGTATATCGGTTACGcgccg                                                                                >  8:771734/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGGAGGGCATTAGTATATCGTTTACGcgccg                                                                                >  8:284398/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGGGGTTCGGATATAG‑GCTGGGTGGTTGGGCATTCGTATATAGTTTTCGcgccg                                                                                >  1:991585/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACATCGATGGACACCATTTTTGGCGGCAAAGCTGGCGGCGTGGTCT‑GTTTTTCATCGGATAACGAGCCAGGTGGGTGGGCAATCCTAAAGCTTTTACGcgcc                                                                                 >  5:966092/1‑140 (MQ=255)
                                                          tgCAGCGTGATGGCGGCGGATATGCGGTGGCGCTGGCGACCTCGCTGGCCACCAATTGTGCCGGCAAAGCGGGCGGCGTGGTCT‑TTTGTGGTTCGGATATAC‑GCTGGGGTGTAGGGCATTCGTATATAGTTTAccctccc                                                                                >  6:733279/1‑134 (MQ=255)
                                                           gCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGT‑TTTGTGGTTGGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGcgccgc                                                                               >  6:926323/1‑140 (MQ=255)
                                                            cAGCTTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCG‑TTTTTGCTTCGGATTTCG‑GCTGGGTTGTAGGGCATTCGTATATCGTTTACgcgcag                                                                                >  4:1052386/1‑136 (MQ=255)
                                                            cAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTTTGCTTCGGATATCG‑GCTGGGTGGTTGGGCATTCGTATATCGTTTACGcgccg                                                                                >  4:299065/1‑138 (MQ=255)
                                                            cAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGcgccg                                                                                >  4:1115596/1‑138 (MQ=255)
                                                            cAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGGGCTTCGGATATCG‑GCTGGGTTGTAGGGCATTAGTATTTCGGTTACGcgccc                                                                                >  4:590470/1‑137 (MQ=255)
                                                            cAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCAATTTTGGCGGCACAGCGGGCGGCGGGTTGG‑TTTGTTGTTCGGAAATCG‑GGAGGGTGGTAGGGCCTACGTATAACCTTTACCccccg                                                                                >  3:436945/1‑138 (MQ=255)
                                                             aGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTGCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCgctg                                                                             <  1:137765/140‑1 (MQ=255)
                                                               cGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTAGGATATCG‑GCTGGGTGGTAGGGCATTCGTTTATCGTTTACGCGCCgcagcg                                                                           >  6:1124552/1‑139 (MQ=255)
                                                                 tGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGCCACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGGTATAG‑GCTGGGTGGTTGTGCATTCGTATATAGTTTAAGCCCCcctgcg                                                                           >  3:1196879/1‑137 (MQ=255)
                                                                 tGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGAATTCGTATATCGTTTACGCGCCGCTGCTgg                                                                         >  8:741694/1‑140 (MQ=255)
                                                                   aTGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTTTGCTTCGGTTATCG‑GGAGGGTGGTAGGGCATTCCTATTACGTTTAAGCGCCGCTGCTgg                                                                         >  4:268078/1‑138 (MQ=255)
                                                                   aTGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTAGGCTATAG‑GCGGGGTGGTTGGGGATTCGTTTTTCGTTTACGCGCCGCTGCggg                                                                         >  3:322985/1‑138 (MQ=255)
                                                                      tCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGCGTTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCgggg                                                                    <  2:736011/140‑1 (MQ=255)
                                                                         gcggATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTAT‑TTTTTGGTTCGGAGATCG‑GCTGGGTGGGATGGCCTTCGTATTTCGGTTACGCGCCGCGGCGGGCGGGGATg                                                                 >  6:151549/1‑140 (MQ=255)
                                                                           ggATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGc                                                                                                                                  <  4:189238/74‑1 (MQ=255)
                                                                               atGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACt                                                           <  1:488311/140‑1 (MQ=255)
                                                                                 gCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACt                                                           <  3:649956/138‑1 (MQ=255)
                                                                                  cGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGG‑TTTTTGCTTAGGTTATAC‑GCGGGGTGGTAGGGCATTGGTTTTTAGTTTACGCGCCGCGGCTTGCGGGGGTGGCGACTAta                                                        >  5:702204/1‑139 (MQ=255)
                                                                                    ggggCGCTGGCGACCGCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGt                                                      <  1:864990/138‑1 (MQ=255)
                                                                                       gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGGGGGGATGGCGACTATCGTTTa                                                   >  7:849242/1‑140 (MQ=255)
                                                                                       gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGT‑TGTGTGGGTCGGATATCG‑GGTGGGTGGTAGGGCATTCGTAGATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACGATCGTTTa                                                   >  8:946468/1‑140 (MQ=255)
                                                                                       gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCT‑TTTTTGCGTAGGCTTTCG‑GCTGGGTGGTAGGGCAGTAGTTTATCCTTTACGCGCCGCGGCTGGCGGGGCGGGCGCCTATCGTTTa                                                   >  5:225749/1‑140 (MQ=255)
                                                                                       gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGAGGCGTGTTCG‑TTTGTGATTCGGATATCG‑GCTGCGTGGTAGGGCATTCGGATATAGTTTAAGCGCCGCTGCTGGCGGGGCTGGCGACGATCGTTTa                                                   >  2:600226/1‑140 (MQ=255)
                                                                                       gcgcTGGCGACCTCGATGGACACCATTTTTGGC‑GCAAAGCGGGCGGCGTGTTGT‑TTTGTGCGTAGGATATAG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCGGGCGGGGATTGCGCCAATCGTTTAc                                                  >  5:986730/1‑140 (MQ=255)
                                                                                         gcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GGTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGTTGGCGACTATCGTTTa                                                   >  4:861552/1‑138 (MQ=255)
                                                                                         gcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACg                                                 <  5:1108349/140‑1 (MQ=255)
                                                                                                 ccTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTg                                         <  7:24349/140‑1 (MQ=255)
                                                                                                 ccTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTg                                         <  5:1192043/140‑1 (MQ=255)
                                                                                                 ccTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTg                                         <  5:150009/140‑1 (MQ=255)
                                                                                                     gATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGa                                     <  7:899147/140‑1 (MQ=255)
                                                                                                       tGGACACCATTTTTGGCGGCAAAGCGGGAGGCGTGTGAT‑TTTTTGCTTAGGATATCG‑GCGGGGGGGTAGGGCCTTCGTAGATCCTTTAAGCGCCGCGGCTGGCGGGGATGGCGACCATCGATTTCGACGGCTTGCCGccc                                   >  6:1185612/1‑140 (MQ=255)
                                                                                                            acCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGGAGGGCATTCGTATATCGTTTACGCGCCGCTGCGGGCGGGGATGGCGCCTATCGTTTACGAAGGATTGCCGACCTGGcc                              >  2:1133443/1‑140 (MQ=255)
                                                                                                            acCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCT‑TTTGTGGTTCCGGTATCG‑GCTGGGGGGTAGGGCAGTGGTATATCGTTTACGCGCCGCTGCGGGCGGGGGTGGCGCGTATCGTTTACGAAGGGTTGCCGACCTGGcg                              >  7:1248719/1‑139 (MQ=255)
                                                                                                             ccATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGc                               <  3:838880/138‑1 (MQ=255)
                                                                                                               cattttGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCGTCGGATATCG‑GGTGGGTGGTAGGGCATTCGTATATAGTTTACGCGCCGCTGCGGGCGGGGATGGCGACTATCGTTTAAGAAGGATTGCCGCCCTGGCCGGa                           >  6:1037145/3‑140 (MQ=255)
                                                                                                               aTTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCg                             <  4:918279/138‑1 (MQ=255)
                                                                                                               aTTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGa                           <  1:285680/140‑1 (MQ=255)
                                                                                                                    tGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTgcg                      <  7:48826/140‑1 (MQ=255)
                                                                                                                                gggcggCGTGGGCT‑TTGGTGCGGCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCGGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGa          <  5:1074777/140‑1 (MQ=255)
                                                                                                                                       gtgtTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGAAAAtt     >  4:1142453/1‑138 (MQ=255)
                                                                                                                                          |                                                                                                                                           
TCCAACGAAACCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCT‑TTTGTGCTTCGGATATCG‑GCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGAAAATTGTC  >  NZ_CP022686/3928207‑3928482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A