Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F36 I0 R1 179 8076.5 8499890 98.3% 8355391 135.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,928,345 T→G 9.0% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,928,3450TG 9.0% 344.6 / 3.9 170F277V (TTC→GTC) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (74/76);  new base G (18/2);  total (92/78)
Fisher's exact test for biased strand distribution p-value = 5.43e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 172 total aligned reads displayed.

CGAAACCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGAAAATTGTCGA  >  NZ_CP022686/3928212‑3928484
                                                                                                                                     |                                                                                                                                            
ggAAACCTCCTTCATTCTCTACACCTCCGGCACGACCGGCAAACCTAATGGTGTGCAGCGTGATGTCGGCTGATAGGATGTGGCGCTTGCGACCTCGATGGACACCATTTTTGTCGTCAAAGCGGGCGGCGTGTTCtt                                                                                                                                          <  7:779177/137‑1 (MQ=255)
           gCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGGGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGat                                                                                                                             <  3:372674/140‑1 (MQ=255)
                ctctACACCTCCGGCACGACCTGCAAACCTACAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑c                                                                                                                         <  8:751202/138‑1 (MQ=255)
                ctctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑Cgg                                                                                                                       >  2:765272/1‑140 (MQ=255)
                ctctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑Cgg                                                                                                                       <  1:20537/140‑1 (MQ=255)
                ctctACAACTCCGGCACGACCGGCAAACCTATAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑Cgg                                                                                                                       <  1:160431/140‑1 (MQ=255)
                 tctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTGTGCTTCGGATAT‑Cggg                                                                                                                      >  4:392847/1‑139 (MQ=255)
                 tctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGc                                                                                                                      >  3:81668/1‑140 (MQ=255)
                 tctACACCTCCGGCACGACCGGCAAACATAAAGGTGTGCAGCGTGATGTAGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTAGCCCGCAAAGCGGGCGGCGTGTTCGTTTGTGCATCCGATAT‑CGGc                                                                                                                      >  6:614327/1‑140 (MQ=255)
                  ctACACCTCCTGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGGCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑Cgg                                                                                                                       <  8:868189/138‑1 (MQ=255)
                  ctACACCTCCGGCACGACCGGCAAACCTACAGGTGTGCAGCGTGAGGTCGGCGGATATGCGGGGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCt                                                                                                                     <  2:213431/140‑1 (MQ=255)
                  ctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCt                                                                                                                     <  3:493424/140‑1 (MQ=255)
                  ctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCGTTTGTGCTTCGGATAT‑Cgg                                                                                                                       >  7:449332/1‑138 (MQ=255)
                  ctACACCTCCGGCACGAACGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCt                                                                                                                     <  2:84932/140‑1 (MQ=255)
                   tACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGGCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTg                                                                                                                    <  2:920146/140‑1 (MQ=255)
                     cacCTCCGGCACGCCCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATCTGCGGTGGCGCTGGCGACATCGATGGACCCCTTTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGTTAT‑CGGCTg                                                                                                                    >  8:125574/1‑138 (MQ=255)
                     cacCTCCGGCACGACCGGGAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACAATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTggg                                                                                                                  <  6:287610/140‑1 (MQ=255)
                        cTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGAGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTTggagg                                                                                                               >  6:193274/1‑140 (MQ=255)
                          ccGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCGTTTGTGCTTCGGATAT‑CGGCTGggtggtt                                                                                                             >  3:991420/1‑139 (MQ=255)
                           cGGCACGACCGTCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTTGCGCTGGCGACCTCGATGGACCCCATTTTTGGCGGCAAAGCGGGCGGCGTGTTATTTTTTGCTTCGGAGAT‑CGGCTGggttctag                                                                                                            >  4:593839/1‑135 (MQ=255)
                           cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGggtggt                                                                                                              <  7:219417/138‑1 (MQ=255)
                           cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTAGGATAT‑CGGCTGGGTGGTAg                                                                                                            >  6:103765/1‑140 (MQ=255)
                           cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTATTTTGTGCTTCGGATAT‑CGGGTGGGTGGTAg                                                                                                            >  3:437633/1‑140 (MQ=255)
                           cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGTTGTTCGGATTT‑AGGGTGggtggttg                                                                                                            >  3:1066471/1‑138 (MQ=255)
                           cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGGGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACAATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTGTTGGTGTTCCGTGAT‑GCGCTGggtggttg                                                                                                            >  3:229413/1‑138 (MQ=255)
                             gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCTGCAAAGCGGGCGGCGGGGTGTTTTGTGCTGCTGATAT‑CGGGTGGGGGGTAg                                                                                                            >  8:129188/1‑138 (MQ=255)
                             gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGGTTTGTTGGTGGGATTT‑CGGGTGGGTGGTAggg                                                                                                          >  1:824528/1‑140 (MQ=255)
                             gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCtt                                                                                                                                  >  5:1045945/1‑117 (MQ=255)
                             gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAggg                                                                                                          <  6:623006/140‑1 (MQ=255)
                             gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAg                                                                                                            <  8:206599/138‑1 (MQ=255)
                             gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAg                                                                                                            <  8:437842/138‑1 (MQ=255)
                             gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTGTTGTGCTTCGGATAT‑CGGCTGggtggttg                                                                                                            >  7:187287/1‑136 (MQ=255)
                             gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGTGCTTGGGGTTT‑AGGGTgggggggtg                                                                                                            >  7:51135/1‑135 (MQ=255)
                             gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGGGTTCTTTTGTGCTTCGGATAT‑CGGATGTGTGGTat                                                                                                            >  8:315216/1‑137 (MQ=255)
                             gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTAGATGGACACCATTTTTGGCGGCAAAGCGGTCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAggg                                                                                                          <  2:585776/140‑1 (MQ=255)
                             gCACGACAGGCAAACCTAAAGGTGTGCAGCGTGATGGCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAggg                                                                                                          <  4:299249/140‑1 (MQ=255)
                             ccACGCCCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGGGTTCTTTTGTGATTCGGATAT‑CGGCTTGGTGGTAggg                                                                                                          >  4:39073/2‑140 (MQ=255)
                               aCGACCGGCAAACCTAACGGGGTGCAGCGTGATGGCGGCGGATATGCGGTGGCGCTGGCGACCTCGAGGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCa                                                                                                        <  6:886518/140‑1 (MQ=255)
                               aCGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGTCGGCGTGTTCTATTGTGATAATGATAT‑CGGCAGGGTGGTAggg                                                                                                          >  7:101659/1‑138 (MQ=255)
                               aCGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAggg                                                                                                          <  7:628211/138‑1 (MQ=255)
                               aCGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCa                                                                                                        <  4:46166/140‑1 (MQ=255)
                               aCGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCa                                                                                                        <  6:813868/140‑1 (MQ=255)
                                  aCCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAACGCGGGCGGCGTGGTCTTTTGTGCATCGGATAT‑GGGCTGGGTGGTTGGGCAttg                                                                                                     >  1:1100984/1‑139 (MQ=255)
                                  aCCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑Cgg                                                                                                                       <  6:715480/122‑1 (MQ=255)
                                  aCCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑Cgg                                                                                                                       >  5:715480/1‑122 (MQ=255)
                                  aCCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGCAAACGCGGCCGGCGGGTTATTTTGGGATTCGG‑TATACGGCTGGGTGGATGGGCAttg                                                                                                     >  1:443851/1‑139 (MQ=255)
                                    cGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGAGGCAAAGAGTGAGGCGTGTTCTTTTGTTCTTCGGATAT‑AGTCTGGGTGGTAGGGAAttg                                                                                                     >  8:465223/1‑137 (MQ=255)
                                     ggCATACCTAACGGTGTGCAGCGTGATGGCGGCGGTTATGCGGTGGCGCTGGCGACCGCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGta                                                                                                  <  2:640253/140‑1 (MQ=255)
                                     ggCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCg                                                                                                    <  8:583640/138‑1 (MQ=255)
                                     ggCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGta                                                                                                  <  4:577888/140‑1 (MQ=255)
                                        aaaCCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTatat                                                                                               <  3:746885/140‑1 (MQ=255)
                                               aaGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTGTGCTTCGGATAT‑AGGCTGGGGGGGAGGGCCATCGTATATAGtttta                                                                                        >  5:139711/1‑138 (MQ=255)
                                                   ggtgCAGCGTGATGTCGGCGGAGATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACgcgc                                                                                    <  4:134345/139‑1 (MQ=255)
                                                     tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGCTGGACACCATTTTTGGCGGCAAAGCGTGAGGCGTCTTGAATTGAGCTTAGGAGAT‑CAGCTGGGGGTGTGGGGATTAGTAAATAGTTTACGccccc                                                                                  >  5:315987/1‑139 (MQ=255)
                                                     tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGCCACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGTGCTTAGGATAT‑CGGCTGGGTGGTTGGGCATTAGTATATGGTTTACGccccg                                                                                  >  3:22481/1‑140 (MQ=255)
                                                     tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCTAAGCGGGCGGCGTGGTGTTTTGTGGTTGGGATTT‑AGGCGGGGTGGTAGGGCCATAGTATATCGTTTACGcgccc                                                                                  >  5:110184/1‑139 (MQ=255)
                                                     tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGGTTTGTGGTTCGGTTAT‑GGGGGGGGTGGTAGGGGCTTCGTATATGGTTTACGcgccc                                                                                  >  3:407345/1‑139 (MQ=255)
                                                     tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTGGGATTT‑CGGCGGGGTGGTAGGGCGATCGTATATCGTTTACGcgccg                                                                                  >  5:901993/1‑140 (MQ=255)
                                                     tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACGcgccg                                                                                  >  2:249491/1‑140 (MQ=255)
                                                     tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACGcgccg                                                                                  <  1:611380/140‑1 (MQ=255)
                                                     tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGGAGATCGTTTACGcgccg                                                                                  >  2:453337/1‑140 (MQ=255)
                                                     tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCGTTTGTGGTTCGGATAT‑CGGCTGGGTGGTTGGGCATTCGTATATGGTTTACGcgccg                                                                                  >  4:158286/1‑140 (MQ=255)
                                                     tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCGTTTGTGCTTCGGGTAT‑AGGCTGGGTTGTAGGGCAGTCGTTTATAGTTTAAGcgccc                                                                                  >  5:9201/1‑139 (MQ=255)
                                                     tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCGTTTGTGCTTCGGATTT‑CGGCGGGGTGGTAGGGGATTCGGATATGGTTTACGcgccg                                                                                  >  2:473457/1‑140 (MQ=255)
                                                     tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCGTTTGTGCTTCGGATAT‑AGGGTGGGTGGTAGGGCATTCGTAAATCGTTTACGcgccc                                                                                  >  6:70155/1‑139 (MQ=255)
                                                     tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGGGGTCGTTTGTGCTTAGGGTAT‑AGGCTGGGTGGTTGGGGATTAGGTTATAGGTTACGcgccg                                                                                  >  1:192525/1‑140 (MQ=255)
                                                     tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACAATTTTTGGCGGCAAAGCGGGCGGCGTGTTCGTTTTTGGTTCGGATAT‑CGGCTGGGTGGTAGGGCATTAGGATATCGTTTAAGcgccg                                                                                  >  1:1068128/1‑140 (MQ=255)
                                                     tgCAGCGTGATGGCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACgcgc                                                                                    <  8:249836/138‑1 (MQ=255)
                                                      gCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGCCCTCGATGGACAACATTTTTGGCGGCAAAGCGGGCGGCGTGTTATTTTGATGTTACGATAT‑AGGGTGGGGGGGAGGGCATTAGTATATCGTTTACGcgcccc                                                                                 >  1:304756/1‑138 (MQ=255)
                                                      gCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGGTTCGGGTAT‑CGGCTGGGTGGTAGGGCATTCGTATATAGTTTACGcgccgc                                                                                 >  3:558542/1‑140 (MQ=255)
                                                       cAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTGTGGTTCGGATAT‑AGGCTGGGTGGTAGGGCATTCGTACATCGTTTACGcgccg                                                                                  >  7:264541/1‑138 (MQ=255)
                                                       cAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTATGGCATTCGTATATCGTTTACGcgccg                                                                                  >  8:309540/1‑138 (MQ=255)
                                                       cAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACGcgccg                                                                                  >  8:666381/1‑138 (MQ=255)
                                                       cAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACGcgccg                                                                                  >  8:163610/1‑138 (MQ=255)
                                                          cGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTGTTGTGGTTCCGATAT‑CGGCTGGGTGGTAGGGGATTGGGATATCGTTTACGCGCCgctgcg                                                                             >  4:1069845/1‑139 (MQ=255)
                                                            tGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGTGGTTCGGTTAT‑AGGGTGGGTGGTAGGGCAATAGTATTTAGTTTAAACCCCGCAGCTgg                                                                           >  5:489162/1‑140 (MQ=255)
                                                            tGATGTCGGCGGATATCCGGTGGCGCTGGCGACCTCGATGGACCCCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTgg                                                                           <  1:835966/140‑1 (MQ=255)
                                                                 tCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGCCACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTGTGGTTCGGATTT‑AGGGTGGGTGGTATGGCATTCGTATATAGGTTACGCGCCGCTGCTGGCgg                                                                        >  7:346876/1‑138 (MQ=255)
                                                                 tCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCgggg                                                                      <  4:1062056/140‑1 (MQ=255)
                                                                 tCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGTGCTTCGGCTAT‑CGGCGGGGTGGTAGGGCATTCGTATATAGTTTACGCGCCGCTGCTGGCgggg                                                                      >  5:677033/1‑140 (MQ=255)
                                                                   ggcggATATGCTGTGGCGCTGGCGACCGCGATGGACACCATTTTTGGCGGCTAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGAt                                                                    <  6:142302/140‑1 (MQ=255)
                                                                   ggcggATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTGCGGATAT‑CGGCTGGGTGGTCGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCgggg                                                                      <  7:968486/138‑1 (MQ=255)
                                                                    gcggATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGGGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATg                                                                   >  4:878058/1‑140 (MQ=255)
                                                                        atatGCGGTGGCGCTGGCGACCGCGATGGACACCATTTTTGGCGGAAAAGCGGGCGGCGTTTTATTTTTTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTAACGCGCCGCTGCTGGCGGGGATGGCGa                                                               <  5:220746/140‑1 (MQ=255)
                                                                               gTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTGTGCTTGGGGTTT‑CGGCTGGGTGGTTGGGGAGTAGTTGATCGTTTACGCGCCGCGGCTGGGGGGGCTGGCGACGATctg                                                        >  5:459356/1‑138 (MQ=255)
                                                                                  gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTa                                                     >  2:1088255/1‑140 (MQ=255)
                                                                                  gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGGGCTTCGGGTAT‑CGGCTGGGTGGTTGGGCATTCGTATATGGTTTACGCGCCGCTGGGGGCGGGGGTGGCGCCTATCGgttt                                                     >  4:26123/1‑139 (MQ=255)
                                                                                    gcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACg                                                   <  1:7973/140‑1 (MQ=255)
                                                                                    gcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGGGTTGTTTTGTGGTTCGGATAT‑AGGGTGGGTGGTAGGGCATTCGTATTTGGTTTACGCGCCGCTGCTGGCGGGGATGGCGGCTATCGgttt                                                     >  8:827046/1‑137 (MQ=255)
                                                                                       ggCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAg                                                <  1:464510/140‑1 (MQ=255)
                                                                                            ccTCGATGTACACCATTTTTGGCGGAAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTTGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTg                                           <  5:614327/140‑1 (MQ=255)
                                                                                                     acacCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTgg                                  <  1:232350/140‑1 (MQ=255)
                                                                                                           tttttGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTAGGATAT‑CGGGTGGGTGGTTGGGCATTCGTATATAGGTTTCGCGCCGCGGCGTGGGGGGATGGCGACGATCTTTTACGAAGGATTTCCGACCTGGCCgggc                            >  6:116699/1‑138 (MQ=255)
                                                                                                            ttttGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACt                           <  4:81668/140‑1 (MQ=255)
                                                                                                            ttttGGCGGCAAAGCGGGCGGCGTGTTCTTATGTGCGTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGa                             <  8:983796/138‑1 (MQ=255)
                                                                                                               tgccgtcAAAGGGGGCGGCGTGTTCATTTTTTCTTCGGATTT‑CGGCGGGGTGTTATGTCATTAGTATCTGTTTAACGCGCCGCTGCTGTCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTgcg                        <  2:352947/134‑1 (MQ=255)
                                                                                                               tGGCGGCAAAGCGGGCGGCGGTTTCTTTTGTGCTGCGGATAT‑CGGCTGGGGGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTgcg                        <  5:253617/140‑1 (MQ=255)
                                                                                                                    gcAACGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCGGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTgcggcg                     <  7:790901/138‑1 (MQ=255)
                                                                                                                              cggcgTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGaaaa         <  4:103824/140‑1 (MQ=255)
                                                                                                                                     ttCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGAAAATTGTCga  <  6:836137/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                            
CGAAACCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATAT‑CGGCTGGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGAAAATTGTCGA  >  NZ_CP022686/3928212‑3928484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A