Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F2 I0 R1 252 7301.5 9636800 98.5% 9492248 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,928,345 T→G 10.7% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,928,3450TG10.7% 296.1 / 7.0 177F277V (TTC→GTC) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (77/77);  new base G (21/2);  total (98/79)
Fisher's exact test for biased strand distribution p-value = 1.80e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

AACGAAACCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGAAAATTGTC  >  NZ_CP022686/3928210‑3928482
                                                                                                                                       |                                                                                                                                          
aaCGAAACCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCtt                                                                                                                                        <  6:1184486/140‑1 (MQ=255)
  cGAAACCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCtt                                                                                                                                        >  1:109335/1‑138 (MQ=255)
    aaaCCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGAGGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTtgt                                                                                                                                    <  8:1044192/140‑1 (MQ=255)
       cctcctGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCt                                                                                                                                 <  8:536514/140‑1 (MQ=255)
       cctcctGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGGGGCGCTGGCGACCTCGAGGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTGCGTTtgtg                                                                                                                                   >  2:791577/1‑138 (MQ=255)
        ctcctGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGc                                                                                                                                  <  2:684156/138‑1 (MQ=255)
          cctGCATGCTCTACCCCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCTCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCg                                                                                                                              <  7:1107873/140‑1 (MQ=255)
            tGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCg                                                                                                                              >  1:305745/1‑138 (MQ=255)
               aTTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGat                                                                                                                           >  1:838915/1‑138 (MQ=255)
                ttCTCTACACCTCCGGCACGACCGGCAAACCTATATGTGTGCAGCGTGATGGCGGCTGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATc                                                                                                                        <  3:1193138/140‑1 (MQ=255)
                  ctctACTCCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCgg                                                                                                                      <  5:244544/140‑1 (MQ=255)
                   tctACCCCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCggg                                                                                                                     >  6:501493/1‑139 (MQ=255)
                    ctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCgg                                                                                                                      <  2:305745/138‑1 (MQ=255)
                    ctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCt                                                                                                                    <  5:1128858/140‑1 (MQ=255)
                    ctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCt                                                                                                                    <  4:381365/140‑1 (MQ=255)
                    ctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCt                                                                                                                    <  4:679432/140‑1 (MQ=255)
                    ctACACCTCCGGCACGACCGGCAAACCTAAAGGGGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCgg                                                                                                                      <  2:726082/138‑1 (MQ=255)
                    ctACACCTCCGGCACGACCGGCAAACCTAAAGGGGTGCAGCGTGATGTCGGCGGATATGCGGGGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCt                                                                                                                    <  8:322901/140‑1 (MQ=255)
                     tACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCAGTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGTGCTTCGGATATCggg                                                                                                                     >  2:564091/1‑137 (MQ=255)
                          cTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACTCCATTTTTTGCGGCACAGCGGGCGGCGTGGTGTTGTGTGGGTCGGGTTTCGGCTGggtgg                                                                                                              >  3:618718/1‑140 (MQ=255)
                          cTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGggtgg                                                                                                              <  7:225719/140‑1 (MQ=255)
                          cTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGAGGGCCACCTTTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGGGCTTAGGATTTCGGCTgggggg                                                                                                              >  7:486670/1‑140 (MQ=255)
                           tCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATTCGGTGGCGCAGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTATGTGCTTCGGATATCGGCTGggtgg‑t                                                                                                            >  4:853095/1‑140 (MQ=255)
                            ccGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGGTATCGGCTgggggg                                                                                                              <  2:101468/138‑1 (MQ=255)
                             cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGCCCTCGATGGACACCATGTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTTGGCTTCGGAGATCGGGTGGGGGG‑GAg                                                                                                          >  4:704333/1‑140 (MQ=255)
                             cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGCTGGACACCAGTTTAGGCGGCAAAGCGGGCGGCGTGGTCGTTATTTCGTAGGATAACTGCTGGGGGG‑GAg                                                                                                          >  7:1000498/1‑140 (MQ=255)
                             cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTTTTGGTCGGATATAGGCTGGGTGG‑Tat                                                                                                          >  5:749418/1‑139 (MQ=255)
                             cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGggtgg                                                                                                              >  7:422874/1‑137 (MQ=255)
                             cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAg                                                                                                          >  4:255166/1‑140 (MQ=255)
                               gCACGACCGGCAATCCTACTGGGGTGCAGCGTGTTGTCGGCGGATATGGGGTGGCGCGTTCGACCTCGATGGACACCATTTTTGGCGGCTAAGCGGGCGGAGTGGTCTTTTGTGGTTCGGATTTCGGCTGGGTGG‑TAg                                                                                                          <  2:791297/138‑1 (MQ=255)
                               gCACGACCGGCAAACCTAAAGGTGTGGAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAggg                                                                                                        <  6:303131/140‑1 (MQ=255)
                               gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAg                                                                                                          >  2:381309/1‑138 (MQ=255)
                               gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAg                                                                                                          <  2:1134314/138‑1 (MQ=255)
                               gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑GAggg                                                                                                        >  8:784878/1‑140 (MQ=255)
                               gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGCCGGATATGCGGTGGAGCTGTCGACATAGATGGACACCATTTTTGGCGGCAAAGCTGGAGGCGTGTTATTTTGGGGTTGGGGTATAGGAGGGGTGG‑GAgtt                                                                                                        >  4:1094036/1‑138 (MQ=255)
                               gCACGACCGGCAAACCTAAAGGTGGGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAggg                                                                                                        <  7:711548/140‑1 (MQ=255)
                                  cGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCAt                                                                                                     <  5:388690/140‑1 (MQ=255)
                                    aCCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TTGGGCAttg                                                                                                   >  3:659804/1‑139 (MQ=255)
                                    aCCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTc                                                                                                   >  4:645282/1‑140 (MQ=255)
                                       ggCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTCGta                                                                                                <  3:733052/140‑1 (MQ=255)
                                                aaaGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTCGTATATCGtt                                                                                         <  2:116229/138‑1 (MQ=255)
                                                  aGGTGTGCAGCTTGATGGAGGCTGATATGCGGTGGAGCTGGCGACCTGTATGGACACCATTTTTGTCGGCAAAGCGGGCGGCGTGGTCTTTTGTGCTTCGGATATCGGCGGTGTGG‑TAGGGCATtcgtatatcgtata                                                                                       <  2:101540/138‑1 (MQ=255)
                                                   ggTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTGTTTTGTGGTTGGGGTATCGGCTGGGTGG‑TTGGGGATTAGTATATGGTTTaa                                                                                      >  1:1037417/1‑137 (MQ=255)
                                                     ggtgCAGCGTGATGGCGGCGGATATGCGGTGGCGCGGGCGACCTCGATGGACACCATTTTTGGCGGCAACGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACgcgc                                                                                  <  8:886933/139‑1 (MQ=255)
                                                       tgtaGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTGTTGTTAGGATATAGGCTGGGGGG‑GATGGCATTCGTATATCGTTTAAGcgccc                                                                                >  3:320439/4‑139 (MQ=255)
                                                       tgCAGCGTGATGTCTGCGGATATGCGGTGGCGCTGGCGCCCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTTTGCTTTTTGGCTTAGGATATCTGCTTCGTGG‑GAGTGAATTAGTATATCGTATAATcgccg                                                                                >  8:680207/1‑140 (MQ=255)
                                                       tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTGTGGTTGGGATATCGGCGGGGTGG‑TTGGGGATTAGTATATCGTTTAACcgccc                                                                                >  3:1139494/1‑139 (MQ=255)
                                                       tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTGTGCTTCGGATATCGGCTGGGGGG‑TAGGGCATTCGTATATCGTTTACGcgccg                                                                                >  4:4565/1‑140 (MQ=255)
                                                       tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTGTGCTTCGGATATAGGCTGGGTGG‑GTGGGCATTCGTTTATAGTTTAAGcgccg                                                                                >  5:630327/1‑140 (MQ=255)
                                                       tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTTTGCTTAGGATATCGGCTGGGTGG‑TAGGGCCTTAGTATATAGGTTAAGccccc                                                                                >  5:443046/1‑139 (MQ=255)
                                                       tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGTTATAGGCTGGGTGG‑TAGGGCATTCGTATATAGTTTACGcgccc                                                                                >  4:584748/1‑139 (MQ=255)
                                                       tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGGTGGGTGG‑TAGGGCCTTAGTATATCGTTTACGcgccg                                                                                >  7:861261/1‑140 (MQ=255)
                                                       tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGGATTCGTATATCGTTTAAGcgccc                                                                                >  7:80855/1‑139 (MQ=255)
                                                       tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACGcgccg                                                                                >  8:209778/1‑140 (MQ=255)
                                                       tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACGcgccg                                                                                >  7:147504/1‑140 (MQ=255)
                                                       tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACGcgccg                                                                                >  8:1136507/1‑140 (MQ=255)
                                                       tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGGGG‑GAGGGCATTCGTATATCGTTTACGcgccg                                                                                >  8:1027490/1‑140 (MQ=255)
                                                       tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCGTTTGTGGTTCGGATATCGGCTGGGTGG‑TAGGGCCTTCGTTTATCGTTTACGcgccg                                                                                >  7:668589/1‑140 (MQ=255)
                                                       tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTATTTTGTGCTTAGGATATCGGCTGGGTGG‑TAGGGCATTCGTTTATCGTGTTCGcgccg                                                                                >  3:56911/1‑140 (MQ=255)
                                                       tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTGTTTTGTGCTTCGGATATCGGCTGGGTGG‑GAGGGGATTCGTATATAGTTTACccgccg                                                                                >  7:258352/1‑140 (MQ=255)
                                                       tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGGGTTGTTTTGTGGGTCGGAGTTAGGCTGGGTGG‑TTGGGCCTTCGGGATTAGTTTACGccccg                                                                                >  5:284470/1‑140 (MQ=255)
                                                       tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGGGGTCGTTTGTGCTTCGGCTATAGGCTGGGGGG‑TAGGGCATTCGTATATAGTTTTCGcgccc                                                                                >  7:45189/1‑139 (MQ=255)
                                                       tgCAGCGTGATGTCGGAGGATATGCGGGGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACGcgccg                                                                                <  6:780383/140‑1 (MQ=255)
                                                        gCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTGTGGTTGGGATATCGGGGGGGTGG‑TAGGGCAATAGGTTATCGTTTAAGcgccgc                                                                               >  5:673145/1‑140 (MQ=255)
                                                         cAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGTCAAAGCGGGCGGCGTGTTCTTTTGTTCTTACGTTATACGCTTGGTGG‑TAGTGCATTCGTATATCGATTTAGcgccg                                                                                >  1:693238/1‑138 (MQ=255)
                                                         cAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTTTGGTTAGGATATAGGCGGGGTGG‑TTTGGGAATCGTATTTCGTTTTCGcgccc                                                                                >  1:14525/1‑137 (MQ=255)
                                                         cAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGTTATCGGCTGGGTGG‑TAGGGCATTCGTATATAGTTTACGcgccg                                                                                >  2:927234/1‑138 (MQ=255)
                                                         cAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGTGCTTCGGATATCTGC‑GGGTGGTTAGGTCATTCGTATATAGTTTAAGcgccc                                                                                >  1:1156206/1‑137 (MQ=255)
                                                         cAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGGGTTGTTTTTTTCTTAGGATATGGGCAGGGTGG‑TAGGGCATTGGTAGATAGTTTAAGccccg                                                                                >  2:1077426/1‑138 (MQ=255)
                                                            cGTGATGTCGGCGGATTTGCGCTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGTCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACGCGCCgctgct                                                                           <  5:8621/140‑1 (MQ=255)
                                                              tGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACGCGCCGCTGCTgg                                                                         >  4:493099/1‑140 (MQ=255)
                                                                     ggcggATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGAt                                                                  <  5:512924/140‑1 (MQ=255)
                                                                     ggcggATATGCGGGGGCGCTGGCGACCGCGATGGACACCATTTTTGTCGGCAAAGCGGGCGGCGTGTGCTTTTGTGCTCCGGATCTCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGAt                                                                  <  5:512925/140‑1 (MQ=255)
                                                                      gcggATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGGGG‑GAGGGCATTCGTATAACGGTTACCCGCCGCTGCGGGCGGGGTTg                                                                 >  3:973146/1‑140 (MQ=255)
                                                                      gcggATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTATTTTTTGGTTAGGGTATCGGCTGGGGGG‑GTGGGCCTTCGTATTTCGGTTACGCGCCCGGGCTGGCCGGGagg                                                                 >  7:17757/1‑138 (MQ=255)
                                                                          atatGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATCTCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGa                                                             <  3:403818/140‑1 (MQ=255)
                                                                              gCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGCGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTAt                                                         <  6:734507/140‑1 (MQ=255)
                                                                               cGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCGGGGTGG‑TAGGGGATTCGTATATCGTTTACGCGCCGCGGCTGGCGGGGATGGCGACGAtg                                                        >  8:844458/1‑139 (MQ=255)
                                                                                 gTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGTGCTTCGGATATCGGGTGGGTGG‑TTGGGCATTCGTATATCGTTTACGCGCCGCGGCTGGCGGGGATGGCGACTATCGt                                                      >  4:620051/1‑140 (MQ=255)
                                                                                   ggCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCGTTTGTGCGTCGGATATCGGCTGGGTGG‑TAGGGCCTTCGTATATCGTTTACGCGCCGCTGGTGGCGGGGATGGCGACTATCgg                                                      >  2:146574/1‑137 (MQ=255)
                                                                                    gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATAGGCGGGGTGG‑TAGGGGATTAGTATATCGTTTACGCGCCGCGGCTGGCGGGGATGGCGACTATAGTTTa                                                   >  6:560505/1‑140 (MQ=255)
                                                                                    gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCGTTTGTGGGTCGGATATCGGCGGGGTGG‑GTGGGCATTCGTATTTCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGtttt                                                   >  3:623798/1‑139 (MQ=255)
                                                                                    gcgcGGGCGACCTCGATGGACACCATTTTTGGCGGCAACGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTa                                                   <  5:1181932/140‑1 (MQ=255)
                                                                                      gcTGGCGTCCTCGCTGGACACGATTTTTGGCGTCAAAGCGGGCGGCGTGTGCTTTTGTGGTTCGGATATCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACg                                                 <  5:717242/140‑1 (MQ=255)
                                                                                         ggCGCCCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCGTCGGATATGGGCTGGGTGG‑TAGGGCATTCGTTTATCGTTTACGCGCCGCTGATGGCGGGGGAGGCGACTATCGTTTaagaag                                              >  8:608335/1‑140 (MQ=255)
                                                                                              ccTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTg                                         <  5:74844/140‑1 (MQ=255)
                                                                                                  gATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGcc                                       <  2:528893/138‑1 (MQ=255)
                                                                                                  gATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGa                                     <  8:480986/140‑1 (MQ=255)
                                                                                                         acCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCtt                                                                                                                                        <  4:1115385/35‑1 (MQ=255)
                                                                                                         acCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGGATTAGTATATAGTTTACGCGCCGCTGCTGGCGGGGGTGGGGACTATCGTTTACGAAGGCTTTCCGACCAGGcc                              >  3:374039/1‑140 (MQ=255)
                                                                                                           cATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTGGGATATCGGCGGGGTGG‑GAGGGGATTCGTTTATCGTTTACGCGCCGCGGCTGGCGGGGATGGCGACTATAGTTTACGAAGGATTGCCGCCCTGGcc                              >  2:473460/1‑138 (MQ=255)
                                                                                                              ttttGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGGGTCGGATATCGGCTGGGTGG‑TAGGGGATTCGTATATAGTTTACGCGCCGCTGGGGGCGGGGATGGCGACTATAGTTTACTAATGATTGACGACAAGGCCGGACt                         >  8:95926/1‑140 (MQ=255)
                                                                                                                 gggcggcAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTgcg                      <  4:7479/139‑1 (MQ=255)
                                                                                                                   gcggcAAAGCGGGCGGCGTGGTATTTTGTGGGTCTGATATAGGCTGGGTGG‑TTGGGCATTAGTATATTCTTTAAGCGCCCCTGCGGGGGGGGATGTGGCCGATCGTTTTAGATGGATTGCCGACCTTGCCGGAcggcggg                    >  3:296098/1‑139 (MQ=255)
                                                                                                                     ggcAAAGCGGGCGGCGTGTTATTTTGTGCTTCGGATATCGGCAGGGGGG‑TAGGGCATTCGTATATCGTTTACGCGCCGCTGGGTGCCGGGATGGCGACAATCGTTATAGAAGGAAGTCCCACCAGGCCGGACTggggcgg                  >  8:934575/1‑139 (MQ=255)
                                                                                                                     ggcAAAGCGGGCGGCGGGTTGTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTAGGATATAGTTTACGCGCCGCTGCTGGCGGGGTTGGCGACTTTCGTTTACGAAGGGTTGCCGACCTGGCCCGGCTgcggcgg                  >  6:1129634/1‑139 (MQ=255)
                                                                                                                      gcAAATCGGGCGGCGGGTTATTTTTGGCGGCGGATATCGGCTGGGTGG‑TTGGGCATTCGTTTATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGTCCTGGCCGGAATGCGGCgtg                 <  7:92803/140‑1 (MQ=255)
                                                                                                                      gcAAAGCGGGCGGCGTGTTCTTTGGTGCTTCGGATATGGGCGCGGCTG‑TAGGGCATTCGTATATCGTTGACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCgtg                 <  8:474983/140‑1 (MQ=255)
                                                                                                                           gcgggcggCGGGTTCTTTTGTGCGTCGGATATCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTgtggtg            <  6:273515/140‑1 (MQ=255)
                                                                                                                                    gtgtTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACGCGCCTCTGCTGGCGGGGATGGCGACTATCGTTTACTAAGGATTGCCGACCTGGCCGTACTGCGGCGTGTGGTGGAAAATTGt   <  4:975159/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                          
AACGAAACCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCTGGGTGG‑TAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGAAAATTGTC  >  NZ_CP022686/3928210‑3928482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A