Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F13 I0 R1 230 5035.0 9125430 98.4% 8979423 135.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,928,345 T→G 12.2% F277V (TTC→GTC)  prpE → propionate‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,928,3450TG12.2% 387.6 / 7.3 203F277V (TTC→GTC) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (84/88);  new base G (28/3);  total (112/91)
Fisher's exact test for biased strand distribution p-value = 8.73e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

TCCAACGAAACCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGAAAATTGTC  >  NZ_CP022686/3928207‑3928482
                                                                                                                                          |                                                                                                                                          
    aCGAAACCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCttt                                                                                                                                       >  4:1095749/1‑140 (MQ=255)
     cGAAACCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCtt                                                                                                                                        <  8:829029/138‑1 (MQ=255)
      gcaaCCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCCAAGCGGGCGGCGTGTTCttt                                                                                                                                       >  8:210350/3‑138 (MQ=255)
      gAAAACTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCACAGCGGGCTGCGTGTTCgtt                                                                                                                                       >  8:573496/1‑138 (MQ=255)
          cctcctGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCt                                                                                                                                 >  2:1129692/1‑140 (MQ=255)
           ctcctGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCtt                                                                                                                                <  5:169017/140‑1 (MQ=255)
           ctcctGCAATCTCTACACCTCCGGCACGACCGGCAAACATAAAGGTGTGAAGCGTTATGTCGGCGGATATGCGGTGGCTCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCtt                                                                                                                                <  5:322230/140‑1 (MQ=255)
               tGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTgggt                                                                                                                            >  5:578924/1‑139 (MQ=255)
                  aTTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGatat                                                                                                                         <  4:686303/140‑1 (MQ=255)
                  aTTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGatat                                                                                                                         <  3:758969/140‑1 (MQ=255)
                      tctACACCTCCGGCACGCCCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGGGTTCTTTTGTGCTTCGGATATCggg                                                                                                                     >  4:638354/1‑139 (MQ=255)
                      tctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTAGTTTTTGGGTCGGATATCggg                                                                                                                     >  1:252323/1‑139 (MQ=255)
                      tctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCCGCGGGTTCTTTTGTGCGTCGGatatag                                                                                                                       >  7:818061/1‑136 (MQ=255)
                       ctACGCCTCCGGCACGACCGGCAAACCTACCTGGGTGCAGCGTGATGTCGGCGTATATGCGGTGGCGCTGGCGACCTCGATGGACTCCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCgg                                                                                                                      <  7:691242/138‑1 (MQ=255)
                       ctACACCTCCGGCTCGACCGGAAATCCTTAAGGTGTGCAGCGTGATGTCGGCGGTTATGGGGGGGCGCTGGCGACCTCGATGGACACCATTTTTGGAGGCAATGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCt                                                                                                                    <  4:622674/140‑1 (MQ=255)
                       ctACACCTCCGGCACGACCGTCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCt                                                                                                                    <  1:277705/140‑1 (MQ=255)
                       ctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCTAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCgg                                                                                                                      <  8:594035/138‑1 (MQ=255)
                       ctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCt                                                                                                                    <  1:786304/140‑1 (MQ=255)
                       ctACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGGGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCt                                                                                                                    <  2:607050/140‑1 (MQ=255)
                       ctACACCTCCGGCACGACCGGCAAACCTAAAGGGGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCt                                                                                                                    <  5:457987/140‑1 (MQ=255)
                       ctACACCTCCGGCACGACCGGCAAAACTAAAGGTGTGCAGCGTGATGTCGGCGTTTATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCt                                                                                                                    <  2:365727/140‑1 (MQ=255)
                       atACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCt                                                                                                                    <  6:304180/139‑1 (MQ=255)
                        tACACATCCGGCACGACCGGCAAACCTAAAGGTGTGCAGAGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTTCTTAGGATATAGGCT‑g                                                                                                                  >  2:429185/1‑140 (MQ=255)
                          cacCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCAGTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTTTGCGTCGGATATCGGCT‑g                                                                                                                  >  7:357686/1‑138 (MQ=255)
                             cTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGCACTCGATGGCCACCATTTTTGGCGGCAAAGCTGGCTGAGTGTTCGTATGTGCTTCGGATAGAGGCT‑Gggt                                                                                                               >  8:254106/1‑138 (MQ=255)
                             atCCGGCACCACCGGCAAACCTAAAGGTGTGAAGCGTGATGTCGGCGGAAATGCGGTGGCGCTGGCGACCTCGATGGCCACCAATTTTGGCGGCAAAGCGGGCGGCGTGTGATTTTGTGATTCCGCTATCGGCT‑Ggatgg                                                                                                             >  6:231349/2‑140 (MQ=255)
                               ccGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑Gggtgg                                                                                                             >  7:535789/1‑138 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGTGCGGCGTGGTCGTTTGTGCGTCGGATTTCGGCG‑GGGTGGTAg                                                                                                          >  3:440249/1‑140 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGat                                                                                                                           >  3:608633/1‑124 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTGTTTTGTGGTTCGGTTATCGGGT‑GGGGGGTAg                                                                                                          >  2:947604/1‑140 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTGTTTTGTGCTTCGGATATCGGCG‑GGGTGGGAg                                                                                                          >  6:521348/1‑140 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGTGCTTCGGATATCGGCT‑Gggtggttg                                                                                                          >  2:193854/1‑138 (MQ=255)
                                cGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGGGGTCTTTTGTGGTTGGGATTTAGGCT‑GGGGGGTAg                                                                                                          >  1:162021/1‑140 (MQ=255)
                                  gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑Gggtgggat                                                                                                          >  8:480744/1‑135 (MQ=255)
                                  gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAggg                                                                                                        <  2:627084/140‑1 (MQ=255)
                                  gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAggg                                                                                                        <  2:199907/140‑1 (MQ=255)
                                  gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAggg                                                                                                        <  2:1023507/140‑1 (MQ=255)
                                  gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAggg                                                                                                        <  5:131885/140‑1 (MQ=255)
                                  gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAg                                                                                                          >  8:398183/1‑138 (MQ=255)
                                  gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTAGGCGGCAAAGCGGGCGGCGTGTGCTGTTGTGCTGCGGATATCGGCG‑GGGTGGTAg                                                                                                          >  8:704973/1‑138 (MQ=255)
                                  gCACGACCGGCAAACCTAAAGGTGTGCAGCGTGAAGGCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAggg                                                                                                        <  1:528155/140‑1 (MQ=255)
                                    acaaCCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCa                                                                                                      <  1:469413/137‑1 (MQ=255)
                                    aCGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAggg                                                                                                        <  7:840107/138‑1 (MQ=255)
                                    aCGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCa                                                                                                      <  1:1010481/140‑1 (MQ=255)
                                    aCGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCa                                                                                                      <  1:380297/140‑1 (MQ=255)
                                    aCGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCa                                                                                                      <  1:824947/140‑1 (MQ=255)
                                    aCGACCGGCAAACCTAAAGGTGTGCAGCGTGATGGCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCa                                                                                                      <  4:870957/140‑1 (MQ=255)
                                    aCGACCGGCAAACATAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGTCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCa                                                                                                      <  4:297111/140‑1 (MQ=255)
                                    aCGAACGGCAAACCTAAAGTTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCa                                                                                                      <  3:460330/140‑1 (MQ=255)
                                     ccacgtgCAAACCTAAACGTGTGCAGCGGGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGTTATCGGCT‑GGGTGGTAGGGCAt                                                                                                     <  6:633593/134‑1 (MQ=255)
                                     cGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCAt                                                                                                     <  4:491596/140‑1 (MQ=255)
                                       aCCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTc                                                                                                   >  6:1101436/1‑140 (MQ=255)
                                       aCCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGGGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTTGTAGGGCATTc                                                                                                   >  6:548960/1‑140 (MQ=255)
                                             aaaCCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTatat                                                                                             <  2:935810/140‑1 (MQ=255)
                                                         gtgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTAGGATATCGGCT‑GGGTGGTAGGGGATTCGTATATCGTTTACGcgcc                                                                                 >  1:642833/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGTCGCTGGCGACATCGAAGGACCCCATTTATTGCGGCAAAGCGGGCGGCGTGTTCAGTTTTGCTTCGGTTATCGGCT‑GGTTGGTATGGCATTCGTATTTCCTTAAAGcgccc                                                                                >  6:212138/1‑139 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGCCACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCGTTTGTGAGTCGGATATCGGCT‑GGGTGGTATGGCATTCGTATATCGTTTACGcgccg                                                                                >  4:205411/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACCCCAGTTTTGGCGGCAAAGCGGGCGGCGTGGTGGTTTGTGCTTCGGATATAGGCT‑GGGTGGTATGGCCTTCGTATATCGTTTACGcgccg                                                                                >  6:954691/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTGTGCTTCGGATATGGGGT‑GGGTGGTAGGGCATTAGGATATCGTTTACGcgccg                                                                                >  5:719684/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTGTGCTTCGGATATAGGCT‑GGGTGGTAGGGCATTCGTATATCGTTTACGcgccg                                                                                >  5:68267/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTGGGGGTCGGATATGCGGG‑GGGGGGTAGGGGATTAGTATAACGTTTTAGcgccc                                                                                >  3:1016296/1‑139 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTAGGATATCGGCT‑GGGGGGTAGGGCATTAGTATATCGTTTACGcgccg                                                                                >  3:875928/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTGTTGTGGTTAGGATATCGGCT‑GGGTGGTAGGGCATTCGTTTATAGTTTTCGcgccc                                                                                >  3:191938/1‑139 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTGGTTTTTTCATCGGATAACGGCTGGGGTGGT‑GGGCATTACTATATAGTTTACGcgccc                                                                                >  5:373680/1‑139 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATGGTTTACGcgccg                                                                                >  6:815225/1‑140 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGAATTCGTATTTCGGTTAcggcccg                                                                                >  3:926131/1‑135 (MQ=255)
                                                          tgCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTATTTTGTGCTTGGGGTATAGGCT‑GGGGGGTAGGGCATTGGTATAACTGTTACGcgccg                                                                                >  3:1082418/1‑140 (MQ=255)
                                                           gCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATCGTTTACGcgcc                                                                                 <  7:56353/138‑1 (MQ=255)
                                                           gCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGAGGCAAAGCGGGCGGCGTGGTGTTTTTTTGTTCGGATATCGGCG‑GGGTGGTTGGGCATTCGTTATTAGTTTAAGcgcc                                                                                 >  7:573987/1‑138 (MQ=255)
                                                            cAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGGTATCGGCG‑GGGTGGTTGGGCCTTCGGTTTTCGTTTACGcgccc                                                                                >  7:75934/1‑137 (MQ=255)
                                                            cAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATCGTTTACGcgccg                                                                                >  8:563563/1‑138 (MQ=255)
                                                            cAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTAGTATATAGTTTACGcgccc                                                                                >  7:874949/1‑137 (MQ=255)
                                                             aGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGGTTTGTGGTTAGGATTTCGGGT‑GGGTGGTTGGGGATTCCTTGATCTGTTACGcgccgc                                                                               >  7:967618/1‑138 (MQ=255)
                                                             aGCGTGATGTCGGCGGATATGCGGTGGCGCGGGCGACCTCGATGGACACCATTTTTGGCGGCAACGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATCGTTTACGCGCCgctg                                                                             <  5:553672/140‑1 (MQ=255)
                                                               cGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTGTGGGTCGGTATTAGGCG‑GGGTGGGAGGGCAGTCGTATATACTTTACGcgccgcgggt                                                                           >  1:747996/1‑136 (MQ=255)
                                                                 tGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTGATTTGTGCTTCGGATATAGGCA‑GGGTGGTTGGGCAATCGGATATAGTTTACGCGCCGCtggtgg                                                                         >  6:165132/1‑140 (MQ=255)
                                                                   aTGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGAGGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATCTCGTTTACGCGCCGCTGCTGGCg                                                                       <  5:964463/140‑1 (MQ=255)
                                                                   aTGGCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATCGATTACGCGCCGCTGCTGGCg                                                                       <  5:785739/140‑1 (MQ=255)
                                                                    tGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGGGTTGTTTTGTGCTTCGGGTATCGGCT‑GGGTGGTTGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCgg                                                                      >  5:240100/1‑140 (MQ=255)
                                                                        ggcggATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCgggg                                                                    <  7:398183/138‑1 (MQ=255)
                                                                         gcggATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCtttt                                                                                                                                      <  5:39816/72‑1 (MQ=255)
                                                                         gcggATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCtttt                                                                                                                                      >  6:39816/1‑72 (MQ=255)
                                                                           ggATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTGTTTTGTGCTTCGGATATCGGCT‑GGGTGGTTGGGCATTCGTATATAGTTTACGCGCCGCTGCTGGCGGGGATg                                                                 >  8:413702/1‑138 (MQ=255)
                                                                                tgaggTGGCGATGGCGACCTCGATGGACTCAATTTTTGGCTGCAACGCGGGCTGCGTGTGCTTTTGTGCTTCTGATATCGGAT‑GGGTGGTAGGGAATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGTATGGCGACTa                                                          <  2:1046386/137‑1 (MQ=255)
                                                                                tGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTa                                                          <  2:500744/140‑1 (MQ=255)
                                                                                  cGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCG‑GTGTGGGAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATc                                                        <  3:665819/140‑1 (MQ=255)
                                                                                         gcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTa                                                   >  8:776127/1‑138 (MQ=255)
                                                                                         gcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACg                                                 <  1:87813/140‑1 (MQ=255)
                                                                                         gcTGGCGACCTCGATGGACACCATGTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACg                                                 <  4:395538/140‑1 (MQ=255)
                                                                                           tGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGaa                                               <  4:81106/140‑1 (MQ=255)
                                                                                            ggCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTGGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGa                                                <  7:403755/138‑1 (MQ=255)
                                                                                                    cGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGGTCTTTTGGGCGTGGGATATCGGCT‑GGGTGGGAGGGCATTCGTAAATCGTTTTCGCGCCCCGGCGGGCGGTGATGGCGCCTTTCGGTTAAGAATGAtttgcg                                      >  4:113539/1‑136 (MQ=255)
                                                                                                      aTGGACACCATTTTTGGCTGCAAAGCGGGCGGAGTGTTCTTTTGTGCTTCGGATATAGGCT‑GGGTGGTAGGGCATTCGTATATCGATTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCg                                      <  8:341508/138‑1 (MQ=255)
                                                                                                      aTGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAc                                    <  4:603965/140‑1 (MQ=255)
                                                                                                            acCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGcc                              <  2:330563/140‑1 (MQ=255)
                                                                                                             ccATTTTTGGCGGCAAAGCGGGCGGAGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCg                             <  4:983201/140‑1 (MQ=255)
                                                                                                                 ttttGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATCGTGTACGAGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACt                         <  1:1098755/140‑1 (MQ=255)
                                                                                                                    tGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTg                        <  8:916833/138‑1 (MQ=255)
                                                                                                                         gcAAAGCGGGCGGCGTGGTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATCGTTTCCGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCgtg                 <  3:848881/140‑1 (MQ=255)
                                                                                                                                        tgtTATTTTGTGCTTAGGATATCGGCT‑GGGTGGTATGGCATTAGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACAATAGTTTACGAAGGATTTCCGACCAGGCCGGACTTCGGCGTGTGGGGGAAAAATGta  >  2:287105/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                          
TCCAACGAAACCTCCTGCATTCTCTACACCTCCGGCACGACCGGCAAACCTAAAGGTGTGCAGCGTGATGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCGGCGTGTTCTTTTGTGCTTCGGATATCGGCT‑GGGTGGTAGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGAAAATTGTC  >  NZ_CP022686/3928207‑3928482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A