Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F35 I0 R1 195 10216.4 8585726 98.3% 8439768 135.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,868,753 T→G 18.0% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,868,7530TG18.0% 243.6 / 26.5 143T18P (ACC→CCC) fdoHformate dehydrogenase O subunit beta
Reads supporting (aligned to +/- strand):  ref base T (18/97);  new base G (25/3);  total (43/100)
Fisher's exact test for biased strand distribution p-value = 2.32e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

CCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCGACGAATGGCGTC  >  NZ_CP022686/2868614‑2868892
                                                                                                                                            |                                                                                                                                            
    tGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACCCGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGGGC‑GGGGGtgaat                                                                                                                                          >  2:492232/1‑136 (MQ=255)
        tttGCCGTCGGTATCGCGATTGTCGTTCCACTCTGACCACGCCACCTGACAGGCTTAACAGCCGATACAGGTGGTAAGGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGt                                                                                                                                      <  1:769691/139‑1 (MQ=37)
         ttGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGGCAGACCGtt                                                                                                                                     >  8:868034/1‑140 (MQ=255)
         ttGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCCCCGGAGGCGC‑GGGGGGGAGACCggt                                                                                                                                     >  3:637886/1‑138 (MQ=25)
         ttGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCCCGCCCGAGGCGC‑GGGGGGCAGccccgt                                                                                                                                     >  8:450855/1‑137 (MQ=25)
         ttGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCCCCCCCGTCGCGG‑GGGGGTAAGAcccgt                                                                                                                                     >  4:174141/1‑137 (MQ=21)
                cGGTATCGCGAATGTCGTTCCACTCAGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAAAGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGGGGCGC‑GGGGGGCAGCCCGGTAGGcg                                                                                                                                >  6:797445/1‑138 (MQ=21)
                  ttttCGCGAATGTCGTTCCACTCTGAACACGCCACATGACAGGCTTTACCGCCGATCCAGGTGGTATCGGCGATTAGTTTCGCCACTTCTTCCTGTAAGTCCCGCGCATGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGa                                                                                                                           <  3:859578/137‑1 (MQ=255)
                     tCGCTAATGTCGTTCCACTATTAACACGCCTCCTCACAGGCTTTACATCCGATACCGGTGGTAACGTCGCTGAGTTTCTCCACTTCTTCCTGTAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAacg                                                                                                                        <  3:85529/140‑1 (MQ=255)
                     tCGCGATTGTCGTTCCACTCTTAACACGCCACCTGACAGGCTTTACAGCCTATACCGGTGGTAACGGCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAacg                                                                                                                        <  7:739136/140‑1 (MQ=37)
                     tCGCGAATGTCGGTCCACTCTGAACACGCCACCTGACAGGCGTTACAGCCGATACAGGTGGTAACGGCGATGAGTTTCGCCACTTCTTCCTGGAAGGCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAacg                                                                                                                        <  2:945804/140‑1 (MQ=255)
                     tCGCGAATGGCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGaa                                                                                                                          <  6:442748/138‑1 (MQ=255)
                     tCGCGAATGGCGTGCCACTCTGCACACCCCACCTGACAGGCTGTACTGTCGATACAGGTGGTAACGTCGCTGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAacg                                                                                                                        <  8:845032/140‑1 (MQ=255)
                            tGTCGACCCACTCTGCACACGCCACCAGACAGGCTTTACAGCCGAAACAGCTGGTAACGTCGATGAGATTCGCCCCTTCTACCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATg                                                                                                                 <  3:991136/140‑1 (MQ=255)
                                      cgctcaaCACGCCACCTGACAGGCATTACAGCCGATACCGGTGGTAACGTCTATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCg                                                                                                       <  8:23804/135‑1 (MQ=38)
                                       actGACCACGCCACCTGACAGGCTTTACAGCCGATACAGGCGGTAACGTCGATGAGTTTCGCAACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGa                                                                                                      <  3:943202/139‑1 (MQ=255)
                                         tGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCCCCCCAGAGCCGC‑GGGGGGGAGGCCGATAGTAG‑CGGTACGGCGAAAGATATAATGCGaat                                                                                                    >  7:77951/1‑138 (MQ=18)
                                           aaCACGCCACCTGATATGATTTACAGACTATTCAGGTGTTAACTTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGCGGCGC‑GGGGGGCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGAtt                                                                                                    <  6:925072/138‑1 (MQ=255)
                                           aaCACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCCCGCCGGCGGCGC‑GGGGGTGAGGCCCGTAGTGG‑GGGGGCCACGAAATATATATTggggattt                                                                                                  >  1:385207/1‑133 (MQ=18)
                                            ccacGCCCCCAGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGCTGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTg                                                                                                   <  5:232304/137‑1 (MQ=255)
                                            ccacGCCACCTTACAGGCTTTACTCCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGAt                                                                                                 <  1:553188/139‑1 (MQ=255)
                                            ccacGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGCAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGAt                                                                                                 <  4:252446/139‑1 (MQ=255)
                                            ccacCCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGAt                                                                                                 <  1:916991/139‑1 (MQ=255)
                                            acacTCCACCTGACATGCTTTACAGCCTATACAGGTGGTAACGTCGATGAGTATCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGAt                                                                                                 <  3:118609/140‑1 (MQ=255)
                                            acacGCCACCTGACCGGCTTTACTGCCGATACAGGTGGTACCGTCGATGAGTTTCGCCACTTCTGCCTGGAAGTCCCGCGCCGGAGGCGC‑GGGGGTCAGACCGTTAGCCG‑CGGAACGACGAATGATATCTTGCGATTGAt                                                                                                 <  7:1006385/140‑1 (MQ=255)
                                            acacGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTg                                                                                                   <  6:744661/138‑1 (MQ=255)
                                            acacGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGAt                                                                                                 <  4:991254/140‑1 (MQ=255)
                                            acacGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGAt                                                                                                 <  4:378598/140‑1 (MQ=255)
                                            acacGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGAt                                                                                                 <  3:1028602/140‑1 (MQ=255)
                                            acacGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGAt                                                                                                 <  2:577269/140‑1 (MQ=255)
                                            acacGCCACCTGACAGGCGTTACAGCCGATACAGGTGGTAACGGCGATGAGTCTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGAt                                                                                                 <  8:761430/140‑1 (MQ=255)
                                            acacGCCACATGAAAGGCTTTAACGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGAt                                                                                                 <  8:836565/140‑1 (MQ=255)
                                            acacGCCAACTGCCAGGCTTTCCAGCCTATCCAGGTGGTTACGGCGTTTAGTTTCTCGACTTCTTCCTGGAAGGCCCTCGCCGGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CTGAACGACGAATGATATCTTGCGATTGAt                                                                                                 <  7:677157/140‑1 (MQ=255)
                                            acacGCAAACTGACAGGCTTTAGAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTg                                                                                                   <  6:554973/138‑1 (MQ=255)
                                            acacCCCACCTGACAGGCTTTCCAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGAt                                                                                                 <  7:307087/140‑1 (MQ=255)
                                            aacagCCTCCTTATAGGCTTTTCACCAGATACTGGTGGTAACTTCGAGTAGTGTCGCCAATTCTTCCTGTAAGTCCCGCGACTGTGGCGC‑GGGGGTCATACCGTTAGTCG‑CTGAACGACGAATGATATCTTGCGATTGAt                                                                                                 <  8:135223/136‑1 (MQ=255)
                                             cacGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATa                                                                                                <  3:221337/140‑1 (MQ=255)
                                                gCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCCCGCCGGTGGCGC‑GGGGGGCCGCCCGGTTGTAG‑CGGGGCGCCGAATTATATATTGCGCTTGATTAGc                                                                                             >  1:526771/1‑140 (MQ=18)
                                                gCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCCCGCCGGCGGGGC‑GGGGGGCCGCACGTTTTTAG‑CGGAAACACGAATGATATATTTCGATTTAttaaa                                                                                             >  1:1002917/1‑136 (MQ=18)
                                                  cACCTGACAGGATTTACAGACTATACTGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACTAATGATATCTTGCGATTGATAAGCCa                                                                                           <  8:805092/140‑1 (MQ=255)
                                                   aCCTGACAGGCTTAACAGCCGATTCTGGTGGTAACGTCGATGTGGTTCTCAACTTCTTCCTGGACGACCCCCGCCTGAGGGGC‑GGGGGTCAGAACGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCat                                                                                          <  2:121505/140‑1 (MQ=255)
                                                     cTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCatat                                                                                        <  3:582948/140‑1 (MQ=255)
                                                     cTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGGGGCGC‑GGGGGTGAGGCCGTTTGTCG‑CGGGACGGCGAATTGTATCTTGCGCTTGATAAGCCctat                                                                                        >  2:873477/1‑140 (MQ=25)
                                                     cTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGCGC‑GGGGGGGAGCCCGGTAGTGG‑CGGGACGGCGGATGCTATATTGGGATTGATAAGcgctat                                                                                        >  4:32152/1‑135 (MQ=25)
                                                          cggCTTTACAGCCGATACAGGGGGTAACGTCGAAGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATgtcgt                                                                                   <  2:565755/139‑1 (MQ=255)
                                                          aGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATgtcgt                                                                                   <  2:446575/140‑1 (MQ=255)
                                                          aGGCTTAACAGCCGATACAGGTTGTAACGGCGATTAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATgtc                                                                                     <  5:58622/138‑1 (MQ=255)
                                                          aGGCGTTACAGCCGATACAGGGGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGGCCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATgtcgt                                                                                   <  1:369549/140‑1 (MQ=255)
                                                            gCTTTACTGCCGATTCAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGGCGGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGtct                                                                                 <  3:602940/140‑1 (MQ=255)
                                                            gCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCCCGCCGGCGGCGC‑GGGGGGGAGCCCGGTATTCG‑CGGAACGAAGAATGATATATTTCGTTTTGTAATCCATATttagtca                                                                                 >  8:617657/1‑139 (MQ=21)
                                                                 aCAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCCGCGGAGGCGG‑GGGGGGGCGAACCGTAGTAG‑CGGAACCCCGCATGATAAATTGACCTTTCTAAGCCATAAgtggtcagcg                                                                              >  6:283656/1‑134 (MQ=255)
                                                                      cGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCCCGCCGGAGGCGC‑GGGGGGGAGACCCGTAGTAGCCGGACCGCGCAATG‑TATAATTCGATTGTTAACCCATAAGGTGGCTGCGTTacaac                                                                       >  2:736209/1‑138 (MQ=255)
                                                                       taTACAGGTGGTAACTTCGATTAGTTTCGCTACTTCTTCCTGTAAGTCCGGCGCCTGTTGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTTCGATTTATAAGCAATATGTCGTCTCCGTTagagat                                                                      <  4:924785/139‑6 (MQ=255)
                                                                        aTACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCCCGCCGGAGGCGC‑GGGGGGGAGACAGTTATTAG‑CGGCAAGAAGAAAGATATCTTTCGTATTATTATCCATATTTGGGATCCGtttgaccat                                                                     >  2:34076/1‑133 (MQ=255)
                                                                            cggtggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGTGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCtttt                                                                   <  5:74697/137‑1 (MQ=255)
                                                                            aGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTcc                                                                 <  4:804817/140‑1 (MQ=255)
                                                                             ggtggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCca                                                                <  7:144475/140‑1 (MQ=255)
                                                                             ggtggtAACGTCGATGAGTTTCGCCACTTCTTCCTGCAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCca                                                                <  1:987153/140‑1 (MQ=255)
                                                                              gtggtAACGTCGATGAGCTTCGCCACTTCATCCTGGAAGACCCGCGCCTGAGGAGCAGGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTc                                                                  <  6:193571/138‑1 (MQ=255)
                                                                               tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCcaca                                                              <  4:6145/140‑1 (MQ=255)
                                                                                   aaCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGCGC‑GGGGGTCAGCCCGTTAGTCG‑CGGGACGACGAATGATATCTTGCGATTTTTAATCCATATGTCGTGTCCGTTACACCTTTTACACAttaa                                                          >  8:235910/1‑138 (MQ=255)
                                                                                        cGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGCGC‑GGGGGGCCGACCGGTAGTAG‑CGGAACGACCCATGATATCTTGGGATTGATAAACCAAAATTAGTCACCGGTACACCCTTTACACAATAACaagg                                                     >  8:759855/1‑140 (MQ=255)
                                                                                         taTGAGTTTCGCCACTTCTTCCTGGACGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaagga                                                    <  1:1003175/139‑1 (MQ=255)
                                                                                         gatgatTCTCGCCACTTCTACCAGGAAGTCCCGCGCGTGAGTCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaagga                                                    <  7:952923/140‑1 (MQ=255)
                                                                                         gATGAGTTTCGCCCCTTCTTCCTGGACGTCCCGCGCCTGAGGCGC‑GGGGGGCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaagga                                                    <  8:703748/140‑1 (MQ=255)
                                                                                         gATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaagga                                                    <  3:228696/140‑1 (MQ=255)
                                                                                         gATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaagga                                                    <  2:858564/140‑1 (MQ=255)
                                                                                         gATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaagga                                                    <  3:1048307/140‑1 (MQ=255)
                                                                                         gATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaagga                                                    <  4:290044/140‑1 (MQ=255)
                                                                                         gATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaagga                                                    <  4:367273/140‑1 (MQ=255)
                                                                                         gATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaagga                                                    <  2:826470/140‑1 (MQ=255)
                                                                                         gATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGAGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaagga                                                    <  7:356775/140‑1 (MQ=255)
                                                                                          aggaggctCGCCCCTTCTTCCGGCAAGGCCCCCGCCTGAGGCGC‑GGGGGTCAGACCGGTAGTCG‑CGGAAAGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaaggaa                                                   <  3:80278/133‑1 (MQ=255)
                                                                                                ttCGCCAGTTCTTCCTGGAAGACCCGCGCCGGCGGCGC‑GGGGGTCAGACCGTTAGGCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACtt                                             <  7:365410/140‑1 (MQ=255)
                                                                                                ttCGCCACTTCTTCCTGGAAGTCCCGCGCCTGTGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGAc                                               <  5:1025931/138‑1 (MQ=255)
                                                                                                ttCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACtt                                             <  7:617657/140‑1 (MQ=255)
                                                                                                 tCGCCACTTATTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTa                                            <  4:460469/140‑1 (MQ=255)
                                                                                                  cGCCACTTCTTCCGGGAAGTCCCGCGCCGGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACtt                                             <  6:752072/138‑1 (MQ=255)
                                                                                                       cttcttCCTGGAAGGCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTcc                                      <  2:432814/140‑1 (MQ=255)
                                                                                                         tcttcCTGGAAGTCCCGCGCCGGAGGCGG‑GGGGGGGAGACCGTTATTGG‑CGGGACGACCAATTATATATTTCGATTGATAAGCCATATTTAGTCTCCGGTAAAACATTTTAACAATAACAAAGAAAGACATaaaaacccc                                    >  2:808821/1‑134 (MQ=255)
                                                                                                                       ccGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCg                      <  7:110414/140‑1 (MQ=255)
                                                                                                                            cgggAGGCGC‑GGGGGTCAGACCGTTCGTCG‑CGGAACGACGAATGATATCTTTCGTTTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCa                 <  3:134180/137‑1 (MQ=255)
                                                                                                                              tGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCa                 <  5:744404/138‑1 (MQ=255)
                                                                                                                               gAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCATTTCCACATTAAAAATGAAGGACATAAACTCCCGCGTCTGCGTGTTAGCCTCAccc              >  1:623172/1‑139 (MQ=255)
                                                                                                                                    gc‑gGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCGACGaa         <  4:580219/140‑1 (MQ=255)
                                                                                                                                    gc‑gGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCGACGaa         <  3:358819/140‑1 (MQ=255)
                                                                                                                                    gc‑gGGGGGCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCGACGaa         <  2:737513/140‑1 (MQ=255)
                                                                                                                                     c‑gGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACcgacga          <  6:23281/138‑1 (MQ=255)
                                                                                                                                        ggggTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGTGTCTGCGTGTTCGCATCACCGACGAATgg      >  1:969761/1‑140 (MQ=255)
                                                                                                                                        ggggTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCGACGaaa        >  5:948888/1‑137 (MQ=255)
                                                                                                                                        ggggTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCGACGAATgg      >  8:941352/1‑140 (MQ=255)
                                                                                                                                        ggggTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCGACGAATgg      >  1:360644/1‑140 (MQ=255)
                                                                                                                                        ggggTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCGACGAATgg      >  3:1001569/1‑140 (MQ=255)
                                                                                                                                        ggggTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGAGTCTGCGTGTTCGCATCACCGACGAATgg      >  8:280495/1‑140 (MQ=255)
                                                                                                                                        ggggTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTACACATTCACAAGGAAGGACTTAAACTACGGCGTCTGCGTGTTCGCATCACCGACGAAt        >  5:948891/1‑138 (MQ=255)
                                                                                                                                           gTCAGACCGTTAGTCG‑CGGAAAGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGAATTATACTCCGGAGTATGCGTTTTAGCATCACCGACGAATGGc     <  5:515086/138‑1 (MQ=255)
                                                                                                                                            tCAGACCGTTAGTCG‑CGGAACGACGAATTATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACTTTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCGACGAATGGCGTc  <  3:346516/140‑1 (MQ=255)
                                                                                                                                            tCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCGACGAATGGCg    <  5:1010672/138‑1 (MQ=255)
                                                                                                                                            tCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCGACGAATGGCGTc  <  1:139939/140‑1 (MQ=255)
                                                                                                                                            tCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGGCTGCGTGTTCGCATCACCGACGAATGGCg    <  6:405101/138‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
CCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGC‑GGGGGTCAGACCGTTAGTCG‑CGGAACGACGAATGATATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCGACGAATGGCGTC  >  NZ_CP022686/2868614‑2868892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A