Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F12 I0 R1	158	5737.7	7407908	98.3%	7281973	135.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	3,444,704	T→G	13.3%	T530P (ACC→CCC)	btsT ←	pyruvate/proton symporter BtsT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	3,444,704	0	T	G	13.3%	371.1 / 13.1	220	T530P (ACC→CCC)	btsT	pyruvate/proton symporter BtsT
Reads supporting (aligned to +/- strand): ref base T (76/105); new base G (32/3); total (110/110)
Fisher's exact test for biased strand distribution p-value = 4.37e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

CTGGTTGGAAATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCC  >  NZ_CP022686/3444570‑3444843

      ggAAATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGa                                                                                                                                   <  1:307212/140‑1 (MQ=255)

       taaaTACCGAAGAGCGGCCTCTTGCGCTTATCGCGGCCGAGCGGATAGTCCACGCCCTGATACAGCAGGTAGCCGCAGAGTCGCTCACAGCCCGCAGTACCGAGGATAGCGGCTACCTGAGCGTCGGTTTTTTTCAGGaa                                                                                                                                  <  5:573592/139‑1 (MQ=255)

       gAAATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGaa                                                                                                                                  <  2:806141/140‑1 (MQ=255)

       gAAATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGaa                                                                                                                                  <  4:925394/140‑1 (MQ=255)

            aCCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGa                                                                                                                             <  5:254173/140‑1 (MQ=255)

               gAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGAt                                                                                                                            >  8:716453/1‑138 (MQ=255)

                aaCAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGGTTTTTTCAGGAACGGGATGaa                                                                                                                         >  2:503562/1‑140 (MQ=255)

                  cAGCGGCCACAGGCTCTTAACGCCGCCCTGCTGATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGAAGTACAGATGATACCGGCAACCCGAGAGTCGGTTTTTTTCAGGAACGGGATGaa                                                                                                                         <  7:817521/138‑1 (MQ=255)

                    gCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTa                                                                                                                    <  4:21004/140‑1 (MQ=255)

                    ccGGCCTCAGGCTCTTAACGCCGCCCAGCGGATCGACCACGCACTTATACAGCCGGAAGCCCCACAGACCCACACAGCCCGCAGTACCGATCATACCGTCTAATATATATTATGTTTTTTTCAGGAACGGGATGatttta                                                                                                                     <  2:190372/139‑6 (MQ=255)

                     cGGCCACAGGCTCTTAACGCCGCCCAGCGGATCGACCACGCCCTGATACATCAGGTAGCCCCACAGACCCACACATCCCGCAGTACCGAGGATACCGGCAACCAGAGAGTCGCTTTTTTTACGGACCGGGATCAA‑TTTaa                                                                                                                   >  1:71066/1‑139 (MQ=38)

                     cGGCCACAGGCTCTTAACGCCGCCCAGCGGATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTaa                                                                                                                   >  1:803269/1‑139 (MQ=255)

                       gCCACAGGCTCTTAACGCCGCCCAGCGGATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTAccc                                                                                                                 >  2:324574/1‑140 (MQ=255)

                          acaGGCTCTTAACGCCGCCCAGCGGATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCcagc                                                                                                              <  3:646115/140‑1 (MQ=255)

                                  ttAACGCCGCCCAGCGGATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCt                                                                                                        <  7:46072/138‑1 (MQ=255)

                                  ttAACGCCGCCCAGCGGATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTg                                                                                                      <  4:337710/140‑1 (MQ=255)

                                  ttAACGCCGCCCAGCGGATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTg                                                                                                      <  5:42898/140‑1 (MQ=255)

                                  ttAACGCCGCCCAGCGGATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTg                                                                                                      <  1:117167/140‑1 (MQ=255)

                                          gccCAGCGGATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGGTTTTTTAATGGAAGGGTTGAA‑GTTACCCCGCCGGTCTTGCAGCATaa                                                                                              >  6:877471/1‑140 (MQ=38)

                                           cccAGCGGATCGACCCCGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATa                                                                                               <  7:342916/138‑1 (MQ=255)

                                           accAGCGGATAGACCACGACCTGATCCAGCACGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGAAACCGGCAACCAGATAGTCGGTTTTTTTCAGGAACGGGATTAA‑GTTACCCAGCATGTCTTTCAGCATaaa                                                                                             <  6:50361/139‑1 (MQ=255)

                                            ccAGCGGATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATaa                                                                                              >  8:561971/1‑138 (MQ=255)

                                                  gATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACgg                                                                                                                               >  7:643572/1‑100 (MQ=255)

                                                    tCGACCACGCCCTTATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATCCCGGCAACCTGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGGCTTGCAGCATAAAGCGGCCa                                                                                      <  8:625236/138‑1 (MQ=255)

                                                    tCGACCACGCCATGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGa                                                                                    <  5:306801/140‑1 (MQ=255)

                                                       aCCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGGACGGGATGAA‑GTTACCCAGCAGGGCTTGCAGCATAAAGCGGCCAGAACg                                                                                 >  6:669129/1‑140 (MQ=255)

                                                         cACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACggg                                                                               <  1:553361/140‑1 (MQ=255)

                                                          aCGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTTCCGATGATACCGGCAACCAGAGAGTCGGAGTTTTTAAGGATCGGGAGGAA‑CTTACCCAGCCGGTCATGCAGCATAAAGCGCCCAGCACGGGt                                                                              >  4:247313/1‑140 (MQ=255)

                                                             cccTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTa                                                                             <  7:716453/138‑1 (MQ=255)

                                                             cccTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTAAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTAcc                                                                           >  6:525102/1‑140 (MQ=255)

                                                                 gATACAGCAGGTAGCCACACAGACCCACACAGCCCGAAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGATCTGGTTTAA‑GTTAACAAGCAGGTATTGAATGATAAAACGGCCAAAACTGTAACCCgaa                                                                       >  6:290546/1‑138 (MQ=255)

                                                                        cagGTAGCCCCACAGACCCACACAGCCCGCAGTTCCGATGATACCGGCCACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAgcg                                                                <  5:712873/140‑1 (MQ=255)

                                                                        cagGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAgcg                                                                <  1:402442/140‑1 (MQ=255)

                                                                        cagGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAg                                                                  >  6:2487/1‑138 (MQ=255)

                                                                        cagGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAAACGGAAGAA‑GTTACCCACAACGTCATTCAGCAAAAACAGGCCAGAACGGGTACCCGCaaacatcc                                                                >  3:832533/1‑133 (MQ=255)

                                                                        cagGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTAAGGAACGGGATGAA‑GTTACCCCGCCAGTCTTGCAGCATAAAGCGGCCATAACGGGTACCCCCATACAgcg                                                                >  2:283323/1‑140 (MQ=255)

                                                                        cagGTAGCCCCACAGACCCACACAGCCCGCAGTACAGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGGAAGGGATGAA‑GTTACCCAGCAGATATTGCAGCAAAAAGCGGCCCGAACCGGTACCCCCCACCAgcg                                                                >  4:105668/1‑140 (MQ=255)

                                                                          ggTAGCCCCACAGCCCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTAATGGACGGCATGAACATTACCC‑GCAGGGCATTCACCACAAAGCGGCCCGAAAGGGTCCCCGCGACCAgcg                                                                >  7:545924/1‑138 (MQ=255)

                                                                          ggTAGCCCCACAGACCCACACATCCCGCAGTACCGATGATACCCGCAACCAGAGAGTCGGTGTTTTTAAGGAAACGGATGAA‑ATTACACACCAGGTATTTCAGCATAAAGCCCCCACAACGGGTACCCGCAACCAGcgcg                                                              >  3:743017/1‑140 (MQ=255)

                                                                           gTAGCCCCACAGACCCTCACAGCACGCAGTACCGATGTTACCGGCAACCAGAGAGTCGGTTTTTTTCCTGAACGGGATGAA‑GTTACCAAGCAGGTCTTTCAGAATAAAGCGGCCAGAACGGGTACCCGCATCCAgctc                                                               <  8:242728/138‑3 (MQ=255)

                                                                           gTACCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGGCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAgcgc                                                               <  8:484251/138‑1 (MQ=255)

                                                                            tAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCATAAACGGTACCCGCAACCAGCGCGGt                                                            >  2:603252/1‑140 (MQ=255)

                                                                             ggCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTc                                                           <  5:185690/139‑1 (MQ=255)

                                                                             agccccacagccccacCCCGCCCGCCGTACCGCAGATACCGCCAACCCGAGCGTCGGTTGTTTTCAGGACCGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGCACGGGTACCCGCATCCCGCGCGGTc                                                           <  6:120454/140‑1 (MQ=255)

                                                                             aGCCCCAGAGACCCACAAAGCCCGCAGTACCGATGATTCCGGCAACCAGAGAGTGTGTTTTTTTCAGGAACGGGATGAA‑GTTACCGAGCTGGTCTTGCTGCATAAAGCGGCCAGAACGGGTACCCGCATCCTGCGCgg                                                             <  7:163049/138‑1 (MQ=255)

                                                                             aGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCCGACCGGGTACCCGCATCCCGCGCGGtg                                                           >  6:453881/1‑139 (MQ=255)

                                                                             aGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTc                                                           <  2:793011/140‑1 (MQ=255)

                                                                               ccccACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTAAGGGAAGGGATGTA‑GTTAACCAGAAGGTCTTGAAGCATAAAGCGTGCAGAACAGGTACCCGCAACCAGAGCGGGCAg                                                         >  3:26988/1‑140 (MQ=255)

                                                                                cccACAGCCCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAgg                                                        <  4:546973/140‑1 (MQ=255)

                                                                                cccACAGACCCCAAGAGCCCGCAGTACCGATGATACGGGCAACCAGAGAGTCGGTTTTTTTCAGGACCGGGAGGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAgg                                                        <  2:954956/140‑1 (MQ=255)

                                                                                cccACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAgg                                                        <  1:951891/140‑1 (MQ=255)

                                                                                cccACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGCACCCGCATCCAGCGCGGTCa                                                          <  7:492604/138‑1 (MQ=255)

                                                                                cccACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTGTTTTTCAGGAAAGGGATGAA‑GTTTACCAGCAGGTATTGCAGCATAAAACGGCCAGAACCGGTAACCGCCACCACCGCGGTCAgg                                                        >  5:860510/1‑140 (MQ=255)

                                                                                  cacaGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGAt                                                      <  2:683143/140‑1 (MQ=255)

                                                                                     aGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑TGTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATg                                                     <  7:226092/138‑1 (MQ=255)

                                                                                     aGACCCACAAAGCCCGCAGTACGGATGATACCGGCACCCAGAGAGTCGGTTTTTTTCAGGAACGGGAAGAC‑GGTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATg                                                     <  8:547318/138‑1 (MQ=255)

                                                                                      gACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGa                                                                                                                             >  1:32913/1‑66 (MQ=255)

                                                                                       aaccACACAGGCCTCAGTACAGTTTATACCGGCCACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTATTTCATCATAAAGCGGCCAGAAAGGGTACCCGCATCCAGCGCGGTCAGGATGAACa                                                 <  1:538473/138‑1 (MQ=255)

                                                                                         ccACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGGTTTTTTAAGGCACGGGATTAA‑GTTACCCAGCAGGGATTGCCGCATAAAGCGGCCAGAACGGGTAACCGCATACAGCGCGGTCAGGAAGAACAGt                                               >  4:487817/1‑140 (MQ=255)

                                                                                          cacacaGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTAAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCACCGCGGTCAGGATGAACAGTg                                              >  1:700509/1‑140 (MQ=255)

                                                                                            cactGCCCGCAGTCCCGATGATAGCGGCAACCAGAGAGTCGGGTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATTAACAGTGCt                                            <  3:359637/140‑1 (MQ=255)

                                                                                                 cccGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTGAGGAACCGGGTGAA‑TTTACCCAGCACGTCTTGGAGCAAAAAACGGCCAGCACGGGTACCCCCATCCAGCGCGGTCAGGATGAACAGTGGTTAGaa                                       >  4:471196/1‑140 (MQ=255)

                                                                                                 cccGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGaa                                       <  1:719204/140‑1 (MQ=255)

                                                                                                 cccGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGaa                                       >  4:671839/1‑140 (MQ=255)

                                                                                                 cccGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGaa                                       <  5:462790/140‑1 (MQ=255)

                                                                                                 cccGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGaa                                       <  3:785277/140‑1 (MQ=255)

                                                                                                 cccGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGaa                                       <  4:837421/140‑1 (MQ=255)

                                                                                                 cccGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTAAGGACCGGGATGAA‑GTTACCCCGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCCGGCAGGATGAACAGTGCTTAGac                                       >  6:599555/1‑139 (MQ=255)

                                                                                                 cccGCAGTACCGATGATACCGGCAACCAGAGAGTCGGGTTTTTTCAGGAACGGGATGAA‑TTTACCCCGCAGGTCTTGCAGCATAAAGCCGCCCGAACGGGTACCCGCATCCCCCGCGGGCAGGATGAACAGTGCTTCgca                                       >  5:602357/1‑138 (MQ=255)

                                                                                                 cccGCAGTACCGATGATACCGGCAACCAGAGAGTCGGGTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCCGCGCGGTCAGGATGAACAGTGCTTCGaa                                       >  6:752718/1‑140 (MQ=255)

                                                                                                 cccGCAGTACCGATGATACCGGCAACCAGAGAGTCGGGTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCGTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGaa                                       >  4:802970/1‑140 (MQ=255)

                                                                                                 cccGCAGTACCGATGATACCGGCAACCAGAGAGTCGGGTTTTTTAAGGAACGGGATGAA‑GTTACCCCGCAGGTCATGCAGCATAAAGCGGCCAGAACGGGTACCAGCCTCCAGCGCGGTCAGGATGAACAGTGCTTAGaa                                       >  2:396775/1‑140 (MQ=255)

                                                                                                   cGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTGAGGAACGGGATGAA‑GTTTCCCCGCAGGTCTTGCAGCCTAAAGCGGCCAGAACGGGTACCCGCCACCAGCGCGGTCAGGATGAACAGTGCGTctca                                       >  7:857171/1‑135 (MQ=255)

                                                                                                   cGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGAACa                                     <  3:213139/140‑1 (MQ=255)

                                                                                                   cGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGAACa                                     <  1:502944/140‑1 (MQ=255)

                                                                                                   cGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGAACa                                     <  4:779569/140‑1 (MQ=255)

                                                                                                   cGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTATTGCAGCATAAAGCGGGCAGAACGGGTACCCGCCTCCCGCGCGGGCAGGGTGAACAGTGCTTCGaa                                       >  8:776916/1‑138 (MQ=255)

                                                                                                   cGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAAACGGATGAA‑GTTACCCAGCCGGTGTTGCAGCATAAAACGGCCACAACGGGTAACCCCAACCACCGCGGTCAGGATTGACAGTGCTTAGaa                                       >  7:787232/1‑138 (MQ=255)

                                                                                                     cAGTACCGATGATACCGGCAACCAGAGAGTCGGGTATTTTAAGGACCGGGATGAA‑CTTACCCACCACGTAATACAGCAAAAAGCCGCCAGAACGCGAACCCCCCTCCACCGGGGTCAGGATGACCAGTGATTccataa                                     >  8:467677/1‑133 (MQ=255)

                                                                                                          ccGATTATACCGGCACCCAGAGAGTCGGTTTTTTTCAGGAACGGTATGAA‑GTTACCCAGCAGGTCTTGCTGCATACAGCGGCCAGAACGGGTACCCGCAGCCAGCGCGGGCAGGATGAACAGTGCTTCGAACAGAATa                                <  7:123447/138‑1 (MQ=255)

                                                                                                          ccGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCATAACGGGTACCCTCATCCAGCGCGGTCAGGATGAACAGTGCTTCGAACAGAATAcc                              <  6:769800/140‑1 (MQ=255)

                                                                                                           cGATGATACCGGCAACCAGAGAGTCGGGTTTTTTCAGGAACGGGTTGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGGCAGGATGAACAGTGCTTCGAACAGAATAccc                             >  3:519379/1‑139 (MQ=255)

                                                                                                            gatgatACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGAACAGAATACCGa                            <  4:400360/140‑1 (MQ=255)

                                                                                                             atgatACCGGCAACCAGAGAGTCGGTTTTTTTCCGTAACGTGATGAA‑TTTTGCAAGCAGGTATTGCATGATAAATCGGCCAGACAGGGTTCCCGGCTCCAGCGCGGTCACGAGGAACACTGAATCGAACACAATACCg                             >  8:518673/1‑138 (MQ=255)

                                                                                                             atgatACCGGCAACCAGAGAGTCGGTTTTTTTCAGGa                                                                                                                                   <  3:457933/37‑1 (MQ=255)

                                                                                                             atgatACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGAACAGAATACCGaa                           <  4:832533/140‑1 (MQ=255)

                                                                                                             atgatACCGGCAACCAGAGAGTCGGTTTTTTTAAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGAACAGAATACCGaa                           >  2:181375/1‑140 (MQ=255)

                                                                                                             atgatACCGGCAACCAGAGAGTCGGTTTTTTTAAGGAACGGGAGGAA‑CTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGAACAGAATACCGaa                           >  4:254986/1‑140 (MQ=255)

                                                                                                               gatACCGGCAACCAGAGAGTCGGGTTTTTTAAGGAACGGGATGAA‑GTTACCCAGCAGGGCGTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGAACAGAATACCGaa                           >  8:666221/1‑138 (MQ=255)

                                                                                                                atACCGGCAACCAGAGAGTCGGGTTTTTTCAGGAACGGGGTGAA‑GTTACACAGCAGGTCTTGCAGGAAAAAGCGGCCAGAACGGGGAACCGCAACCAGCCCGGTAACGAATAACATTGCTTCGAACAGCATACCGAAGTg                        >  2:111765/1‑140 (MQ=255)

                                                                                                                  aCCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTg                                                                                                      >  4:712055/1‑60 (MQ=255)

                                                                                                                    cGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGAACAGAATACCg                             <  7:371974/131‑1 (MQ=255)

                                                                                                                    cGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGAACAGAATACCg                             >  8:371974/1‑131 (MQ=255)

                                                                                                                       cAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGAACAGAATACCGAAGTGATACCAg                 <  3:399414/140‑1 (MQ=255)

                                                                                                                       cAACCAGAGAGTCGGTTTTTTTAAGGAACGGGATGAA‑GTTACCCAGCAGGTATTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGAACAGAATACCGAAGTGATACCAg                 >  2:347925/1‑140 (MQ=255)

                                                                                                                                agTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCAACCAGCGCGGTCAGGATGAACAGTGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCaag        >  4:416573/1‑138 (MQ=255)

                                                                                                                                 gTCGGTTTTTTTCAGGAACGGTATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGt       <  1:189032/140‑1 (MQ=255)

                                                                                                                                      tttttttCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGcc  <  2:43498/140‑1 (MQ=255)

                                                                                                                                      tttttttCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGcc  <  6:641050/140‑1 (MQ=255)

                                                                                                                                      tttttttCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGcc  <  3:671839/140‑1 (MQ=255)

CTGGTTGGAAATACCGAACAGCGGCCACAGGCTCTTAACGCCGCCCAGCGGATCGACCACGCCCTGATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAGTCGGTTTTTTTCAGGAACGGGATGAA‑GTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGTGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCC  >  NZ_CP022686/3444570‑3444843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A