Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F12 I0 R1	158	5737.7	7407908	98.3%	7281973	135.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	3,797,481	T→G	15.6%	L130V (TTA→GTA)	CIW80_RS19065 →	LacI family DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	3,797,481	0	T	G	15.6%	197.9 / 8.8	119	L130V (TTA→GTA)	CIW80_RS19065	LacI family DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base T (26/72); new base G (19/2); total (45/74)
Fisher's exact test for biased strand distribution p-value = 5.90e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

CTCCCGCTTTATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGCATCA  >  NZ_CP022686/3797348‑3797618

ctaccGCTTTATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGATACTGCTTGGCTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑gttagtta                                                                                                                                       >  1:620196/4‑140 (MQ=255)

cTCCCGCTTTATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑gtttgt                                                                                                                                         >  8:804316/1‑138 (MQ=255)

cTCCCGCTTTATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑ggtagttt                                                                                                                                       >  4:604304/1‑139 (MQ=255)

  cccGCTTTATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑gtttgtta                                                                                                                                       >  7:625443/1‑138 (MQ=255)

  cccGCTTTATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑ggtagttt                                                                                                                                       >  8:482853/1‑137 (MQ=255)

  cccGCTTTATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTTGTtttt                                                                                                                                     >  6:78876/1‑140 (MQ=255)

  cccGCTTTATGCAGTCGTTGCAGCAGGGGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑gttttttt                                                                                                                                       >  7:152787/1‑133 (MQ=255)

    cGCTTTATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTAtt                                                                                                                                     <  7:424311/138‑1 (MQ=255)

     gCTTTATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGGTAttttt                                                                                                                                  >  1:382425/1‑140 (MQ=255)

        ttctGCAGGCGTTGCAGCAGGGGCTTAATAAAACTGAATTCCCGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAACGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGt                                                                                                                               <  2:715820/137‑1 (MQ=255)

         tATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGtt                                                                                                                              <  2:892901/140‑1 (MQ=255)

              aGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAg                                                                                                                         <  3:452067/140‑1 (MQ=255)

                tCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCACCCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGtt                                                                                                                       >  4:209302/1‑140 (MQ=255)

                tCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTAGTTATTTTTGGTTCTCAGtt                                                                                                                       >  1:766559/1‑140 (MQ=255)

                tCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTAGTTATTTTTGGTTCTCAGtt                                                                                                                       >  3:633795/1‑140 (MQ=255)

                tCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTAGTTATTATTGGTtcgaaagt                                                                                                                       >  4:274596/1‑134 (MQ=255)

                  gTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTTGTTATTTTTGGTTGTGAGtt                                                                                                                       >  8:73370/1‑138 (MQ=255)

                   ttGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGc                                                                                                                    <  1:271284/140‑1 (MQ=255)

                      cagccgGTGCTTAATAAAAATTTATTCCAGCTAAAGCTTGGGTGTCATGAGTATAATCTGCTCACAGAAGCTGATATTCTGATGACGCTTCATCAAGGCAACCCGGAGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCGGa                                                                                                                 <  5:189328/140‑1 (MQ=255)

                        gcagGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATa                                                                                                               <  1:473826/140‑1 (MQ=255)

                         cagGTGCTTAATAAAAATGAAATCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACCCTTCTTCAAGGCAACCCGGCGGC‑GTTAGGTATTTTTGGTAAGCACTTGGCTGagta                                                                                                              >  2:382560/1‑137 (MQ=255)

                           ggTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGGCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATaa                                                                                                              <  8:718828/138‑1 (MQ=255)

                            gTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAAc                                                                                                           <  4:777050/140‑1 (MQ=255)

                              gCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTATTTATTTTTTGTTTTCAGTTTGGAGATAAAAcaa                                                                                                         >  1:638439/1‑138 (MQ=255)

                                   ataaAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTTGTTTTTTTTGGTTCTCAGTTGGCTGATAAAACT‑CAcc                                                                                                     >  8:141426/1‑138 (MQ=255)

                                   ataaAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTAGTTTTTTTTGGGTCTGAGTTGGCTGGTTAAACT‑CCCCAg                                                                                                   >  2:17685/1‑140 (MQ=255)

                                       aaaTGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTTGTTTTTTTTTGTTGTGAGTTGGCTGTTTAAAAA‑GACCAGATaa                                                                                               >  1:692081/1‑139 (MQ=255)

                                              ttCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGATATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTGAGGTATTTTTGGTTGTCCGTTGGCTGGTTAAACT‑CAACAGATACACgagca                                                                                        >  5:396931/1‑138 (MQ=255)

                                              ttCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCgag                                                                                          <  7:581133/138‑1 (MQ=255)

                                              ttCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGaa                                                                                        <  2:593176/140‑1 (MQ=255)

                                              ttCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGaa                                                                                        <  4:511836/140‑1 (MQ=255)

                                              ttCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGaa                                                                                        <  1:697117/140‑1 (MQ=255)

                                              ttCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTTGTTAATTTTTTTTATCACTTAGCTGATAAAAAT‑CACCAGAAACTCGAGaa                                                                                        >  5:699199/1‑140 (MQ=255)

                                              ttCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGAGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCgag                                                                                          <  7:562656/138‑1 (MQ=255)

                                              atCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGaa                                                                                        <  3:677856/139‑1 (MQ=255)

                                                    cTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTTGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACa                                                                                    <  8:625443/138‑1 (MQ=255)

                                                     tACTTCTTGGGGGTCATGAGTATAATCAGCTCAAAGAAGATGAAATTCTGATGACTCTTCTTCAATGCAACCCGGCTGC‑TTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAAAT‑ACCCAGATACTCGAGAAAACAAat                                                                                 <  1:144561/140‑1 (MQ=255)

                                                     tACTGCTTGGGTGTCAAGAGTATACTCAGCTCAAAGACGCTGAAATTCTGATGACGATTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAat                                                                                 <  6:234883/140‑1 (MQ=255)

                                                         gCTTGGGTTTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGCTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATAtac                                                                             <  4:882449/140‑1 (MQ=255)

                                                         gCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAatat                                                                               <  8:15656/138‑1 (MQ=255)

                                                         gCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTATCAATTGGCTGATAAAAAT‑CAACAGATACACGATAAAACAAAaataa                                                                             >  4:559204/1‑139 (MQ=255)

                                                          cgtggtggtCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATTACGCTTCTTCAATGCAACCCGGCGGC‑TTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATAtacc                                                                            <  3:648448/133‑1 (MQ=255)

                                                          catGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCAGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTTATAAAACT‑CACCAGATACTCGAGAAAACAAATAtacc                                                                            <  3:648449/138‑1 (MQ=255)

                                                          cTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGTCGGC‑GTTTTTTAATTTTGGTTGAAAGTTGGGTGTAAAAACT‑AACCAGATACAACAGAAAACAAAtata                                                                              >  7:681820/1‑138 (MQ=255)

                                                          cTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACGAGATACTCGAGAAAACAAATAtacc                                                                            <  3:746883/140‑1 (MQ=255)

                                                          cTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAAtata                                                                              <  7:351915/138‑1 (MQ=255)

                                                          cTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATAtacc                                                                            <  1:91176/140‑1 (MQ=255)

                                                          cTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATAtacc                                                                            <  3:311088/140‑1 (MQ=255)

                                                          cTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGA‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATAtacc                                                                            <  4:395864/140‑1 (MQ=255)

                                                              ggTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTAGTTATTTTTGGTTATAAGTTGGCTGATAAAACT‑CACCCGAGACTCGAGAAAAAAAATAtacat‑a                                                                         >  7:593215/1‑138 (MQ=255)

                                                                tgtCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTgg                                                                                                                                <  8:647543/83‑1 (MQ=255)

                                                                  tCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTTTTTTTTTTTGGTTGTGAGTTGGGTGATTAAAAT‑CACCCGATAAAAGAGAAAACAAATAtaaca‑acccgtt                                                                   >  1:444702/1‑136 (MQ=255)

                                                                  tCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTATGTATTTTTTGTTATCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAAACAATAtaact‑acccgta                                                                   >  4:814262/1‑136 (MQ=255)

                                                                  tCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTAGCAGGTGGCTGATAAAAAT‑CACCACATACTAGAGAAAACAAATATAACT‑ACCGttt                                                                   >  6:219506/1‑139 (MQ=255)

                                                                         tataATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATTAAACT‑CACCACAATCTCGAGAAAACAAATATACCA‑AAGGGTAACGTTGt                                                            >  3:59297/1‑140 (MQ=255)

                                                                         tataATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTGCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGt                                                            <  1:688803/140‑1 (MQ=255)

                                                                           taATCAGCTCAAAGAAGCTGAAATTCTGATGAGGCTTCTTCAAGGCAAGCCGTCGGC‑GTTAGTTATTTTTGGTTCTCTGTTGGCTGATATAACT‑CTCCAGATACTCGAGAAAACAAATATAGCT‑ACCGTTAACGTTGTCg                                                          <  3:289039/140‑1 (MQ=255)

                                                                           taATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCGAGGCACCCCGGCGGC‑GTTAGTTATTTTTGGTTATAAGTTGGCTGGTAAAACT‑CACCCGATACTCGCTAAAACAAATATACCT‑ACCGTTAACGTTGt                                                            >  8:596714/1‑138 (MQ=255)

                                                                           taATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGt                                                            <  7:451041/138‑1 (MQ=255)

                                                                            aaTCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTTGTTATTTTTGGGTGTCAGTTGGCGGGTAAAACT‑CACCAGATTCTCGCGCAAAAAAATATACCT‑ACCGGTTACGGTGTCgg                                                         >  4:203275/1‑140 (MQ=255)

                                                                             aTCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGTC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACTAATATACCT‑ACCGTTAACGTTGTCGGt                                                        <  1:458697/140‑1 (MQ=255)

                                                                             aTCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCg                                                          <  7:436079/138‑1 (MQ=255)

                                                                               cAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTc                                                      <  5:27554/140‑1 (MQ=255)

                                                                               cAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTc                                                      <  1:62355/140‑1 (MQ=255)

                                                                                aGCTCAAAGAAGCTGAACATCTGATGACGCTTCTTAAATGACACACGTCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAACCT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGtt                                                       <  8:652987/138‑1 (MQ=255)

                                                                                 gCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTAGTTTTTTTTGGGTGTCAGTTGGCTGATAAAACT‑CACCCGATACTGGAGAAAACAAATATACCT‑ACCGGTAACGTTGTCGGTTacc                                                    >  4:358011/1‑140 (MQ=255)

                                                                                 gCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGGTGTTTTTTTTGGGTGTCAGTTGGGGGATAAAACT‑CCCCCGATACGAGAGAAAACAAATATAACT‑ACCGTTTAAGTTGTCgggtgcc                                                    >  3:803547/1‑135 (MQ=255)

                                                                                  cTCAAAGAAGCTGAAATTCTGATGCCGCTTCTTCAAGGCAACCCGGCGGC‑GGTAGTTATTTTTTGGTGGCATTTGGCTGATAAAAAA‑CACCACATAATGGTTAAAAAAAATATACCTAACCGTT‑ACGTTTTAGGTTcccc                                                   >  6:908893/1‑140 (MQ=255)

                                                                                  cTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGCGGTTTGTT‑TTTTTGGGTGTGAGTTTGGTGTTTAAACT‑CACCGCGTACTCGAGTAAACAAATATACAA‑ACCCTTAACGGTGTCGGTTgccc                                                   >  6:908894/1‑140 (MQ=255)

                                                                                  cTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTTGTTGTCAGTTGGCTGATAAAAAT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCCTTAAAGTTGTCGGGTaccc                                                   >  2:905970/1‑140 (MQ=255)

                                                                                  cTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTGTGGTATTTTTTGGTTGGAGTTGGCTGGAAACACA‑GCCCCGCATCCAGAGAAAACCAAGATAACT‑ACCGGTAGCGTTTTGGGTTgccc                                                   >  6:367434/1‑140 (MQ=255)

                                                                                  cTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTTGGTTTTTTTGGTTTTGATGTGGGTGTTTAAACTAGGCCAG‑TAATGGAGAGAACAAATATTCAA‑TACGTTTACGTTGTGGGtgagcc                                                   >  5:5001/1‑135 (MQ=255)

                                                                                  cTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTAGTTTTTTTTTGTTCTAAGGTGGGGGATAAAACT‑AACCAGATACACGCGAAAACCAATATAACG‑ACCGTTTACGTTGTGGGTTaccc                                                   >  6:885950/1‑140 (MQ=255)

                                                                                  cTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTAGTTATTTTTTGTTGTCAGTTGGCGGTTAAAACT‑CACCAGATGCTCGAGAAAACAAATATTCCT‑ACCGTTTACGTTGTCGGGTaccc                                                   >  5:587049/1‑140 (MQ=255)

                                                                                   tCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTTTTTTTTTTTGGTGGTCAGTTGGGTGGTAAAACT‑GCCCAGGTTCGCGAGGAAACAAATATAACA‑ACCGGTTACGGTGTGGGTTgcccg                                                  >  6:580885/1‑139 (MQ=255)

                                                                                   tCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GGTTGGTTTTTTTTGTTTTAAGTTTTGTGGTTAAAAA‑CAACCGATTATCGAGGAAAAAAATATTACA‑AACGGTTACGTTGTggggttgcct                                                  >  5:896342/1‑133 (MQ=255)

                                                                                             tGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTTGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCgg                                        <  3:559204/140‑1 (MQ=255)

                                                                                                   tCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCa                                    <  7:240515/138‑1 (MQ=255)

                                                                                                     tgatgaCGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAAtt                                <  2:64688/140‑1 (MQ=255)

                                                                                                     tgatgaCGCTTCTTCAAGGCAACCCGGCGGC‑GGTAGTTATTTTTGGTGCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAAtt                                <  1:235793/140‑1 (MQ=255)

                                                                                                                 ttcAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAAAAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGaaa                      <  8:401768/138‑1 (MQ=255)

                                                                                                                 ttcAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAAAAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACa                    <  4:641672/140‑1 (MQ=255)

                                                                                                                    acggCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCGCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCa                 <  6:605302/138‑1 (MQ=255)

                                                                                                                    aaGGCATCCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCTGCTCAAATTACCATCGAAACAGCa                 <  6:699199/140‑1 (MQ=255)

                                                                                                                    aaGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAg                   <  7:412995/138‑1 (MQ=255)

                                                                                                                           cccGGTGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTg          <  4:655950/140‑1 (MQ=255)

                                                                                                                           cccGGCGGC‑GTTAGTTATTTTTTGGTGTCAGTTGGCTGATAAAACA‑CACCCGATACTCGAGAAAACAAATATACCT‑ACAGTTTACGTTTGGGGTTGCCCGTTGAGTCCGGCGCAAATTAACCAGGGAACAGCAttttttt          >  6:17658/1‑139 (MQ=255)

                                                                                                                            ccGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑AACGTTAACGTTTTGGGTTCCCCTTTGAGGCCGGCTCAAATTAACATCGCAACAGCAtttttt           >  8:376935/1‑138 (MQ=255)

                                                                                                                               gcggc‑gTTATTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGc      <  1:77599/140‑1 (MQ=255)

                                                                                                                               gcggc‑gTTAGTTATTTTTGGTTCTCAGTTTGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGc      <  1:714521/140‑1 (MQ=255)

                                                                                                                               gcggc‑gTTAGTTATTTTTGGTTCTCAGTTGTCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGaa        <  7:773674/138‑1 (MQ=255)

                                                                                                                               gcggc‑gTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGc      <  1:849278/140‑1 (MQ=255)

                                                                                                                               gcggc‑gTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGc      <  1:54655/140‑1 (MQ=255)

                                                                                                                               gcggc‑gTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGc      <  1:269354/140‑1 (MQ=255)

                                                                                                                               gcggc‑gTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGc      <  2:510306/140‑1 (MQ=255)

                                                                                                                               gcggc‑gTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CAACAGATACTCGAGAACACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGc      <  6:248593/140‑1 (MQ=255)

                                                                                                                               gcggc‑gGTAGTTATTTTTGGTTCTAAGTTGGCTGATAACACT‑CACCAGATACTCGAGAATACAAATATACCT‑ACCGTTATCGTTGTCGGTTCCCCTTTCAGTCCGGCTCAATTTACCTTCGAAACAGCATTTTTTGAAGc      <  1:373216/140‑1 (MQ=255)

                                                                                                                                 ggc‑gTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGc      <  8:506133/138‑1 (MQ=255)

                                                                                                                                 ggc‑gTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGCAt    <  4:803547/140‑1 (MQ=255)

                                                                                                                                    ggttagttaTTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATAATCGAGAAAACAAATATTCCT‑CCCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGCATca  <  5:245680/139‑1 (MQ=255)

CTCCCGCTTTATGCAGTCGTTGCAGCAGGTGCTTAATAAAAATGAATTCCAGCTACTGCTTGGGTGTCATGAGTATAATCAGCTCAAAGAAGCTGAAATTCTGATGACGCTTCTTCAAGGCAACCCGGCGGC‑GTTAGTTATTTTTGGTTCTCAGTTGGCTGATAAAACT‑CACCAGATACTCGAGAAAACAAATATACCT‑ACCGTTAACGTTGTCGGTTCCCCTTTCAGTCCGGCTCAAATTACCATCGAAACAGCATTTTTTGAAGCATCA  >  NZ_CP022686/3797348‑3797618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A