Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F35 I0 R1	304	9232.4	10623078	98.3%	10442485	135.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	1,674,244	T→G	11.7%	T182P (ACC→CCC)	ygfB ←	UPF0149 family protein YgfB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	1,674,244	0	T	G	11.7%	449.6 / 13.5	275	T182P (ACC→CCC)	ygfB	UPF0149 family protein YgfB
Reads supporting (aligned to +/- strand): ref base T (66/170); new base G (26/12); total (93/182)
Fisher's exact test for biased strand distribution p-value = 3.34e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

AGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATC  >  NZ_CP022686/1674104‑1674382

       cgcTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTg                                                                                                                                      <  6:151452/140‑1 (MQ=255)

          tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCgg                                                                                                                                   <  2:294614/140‑1 (MQ=255)

          tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCgg                                                                                                                                   <  2:637483/140‑1 (MQ=255)

          tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCgg                                                                                                                                   <  8:526592/140‑1 (MQ=255)

          tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCgg                                                                                                                                   <  5:151645/140‑1 (MQ=255)

          tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCgg                                                                                                                                   <  6:511285/140‑1 (MQ=255)

          tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCgg                                                                                                                                   <  7:513499/140‑1 (MQ=255)

          tGCCGGGTTACATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCgg                                                                                                                                   <  6:1238923/140‑1 (MQ=255)

          tGCCGGGTGGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCCCTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCgg                                                                                                                                   <  8:218387/140‑1 (MQ=255)

          ggCCGGGGTGCATTTGCGCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCgg                                                                                                                                   <  1:712779/139‑1 (MQ=255)

            cggggTTGCTTTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGAt                                                                                                                                 <  8:202573/138‑1 (MQ=255)

            ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGAt                                                                                                                                 <  1:1268221/140‑1 (MQ=255)

            ccGGGTTGCACTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGAt                                                                                                                                 <  2:104468/140‑1 (MQ=255)

            cagggTTGCATTTGCTCTACCAGGGCCTCACGGCGACGCTGAAACTCTTGCCGGGATCTATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGAt                                                                                                                                 <  6:1141438/138‑1 (MQ=255)

                 ttGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTATTCAGGCG‑GGGGCGGTTTGGGATGAGtt                                                                                                                            >  1:879742/1‑139 (MQ=255)

                   gCATTTGCTCTACAAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTTCTTTTTGTG‑CGGGCGGGTGCGGAGGAGtt                                                                                                                            >  3:518444/1‑137 (MQ=255)

                    cATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAg                                                                                                                         <  8:950112/140‑1 (MQ=255)

                      tttGCTCTACCAGGGCCTGACGGCGACACTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAATTATGGCG‑CGGTCGGTTGCGGATTTGTAAATgt                                                                                                                       >  1:194043/1‑140 (MQ=255)

                        tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGTTATATCACTCTTAACACTCTCGTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  6:279924/140‑1 (MQ=255)

                        tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  2:1291296/140‑1 (MQ=255)

                        tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  5:202416/140‑1 (MQ=255)

                        tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  1:1071620/140‑1 (MQ=255)

                        tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGgt                                                                                                                       <  3:895216/138‑1 (MQ=255)

                        tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGgt                                                                                                                       <  3:1192647/138‑1 (MQ=255)

                          ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  4:838534/138‑1 (MQ=255)

                          ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                     <  3:274048/138‑1 (MQ=255)

                          ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                   <  2:210046/140‑1 (MQ=255)

                          ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                   <  7:657500/140‑1 (MQ=255)

                          cgctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAGTTCTGGCG‑CTGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                   <  5:1111388/138‑1 (MQ=255)

                              aCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTTCTTGGGGGGCCGGCGGGTTGCGG‑TGAGTTAAGttgtaggggg                                                                                                               >  5:793345/1‑134 (MQ=255)

                              aCCAGGGCCTGACGGCGACGCTGAAACTCTTGACGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTAGGTTTTTTTACATATGGCG‑AGTTCCGTTGCGGTTGAGTAAAAGTTTCGTggg                                                                                                               >  7:122239/1‑139 (MQ=255)

                                   ggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATACCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTCCTTCTGGCG‑CGGTAGGTGTCGGATGAGTAAAGGTGTCGGTGCATAAc                                                                                                          >  6:446541/1‑140 (MQ=255)

                                    gCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTTCTTGTGGCG‑CGGGGGGGTGGGGATGAGTAAATGTGTCGTGGGATAACa                                                                                                         >  1:1183380/1‑140 (MQ=255)

                                     ccTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTCTTCTGGCG‑CGGGGGGTTGGGGATGAGTAAAGGTGTGGTGGCATaaaac                                                                                                        >  7:204742/1‑137 (MQ=255)

                                      cTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAgc                                                                                                       <  7:212931/140‑1 (MQ=255)

                                         aCGGCGCCGCTGAAACTCTTGCCGGGATATATCACTCAGAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACATATTGCG‑CGGTCGGTGTCGGATGAGTTAAGGTTTCGTGGCATAAAAgcg                                                                                                      >  3:917442/1‑138 (MQ=255)

                                         aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAAGTGTGGCG‑CGGTTTGTTGCGGATGAGAAAAGGGGTAGGTGCAAAACatcccg                                                                                                    >  5:871458/1‑135 (MQ=255)

                                         aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTTCTTATGGGG‑CGGGGGGGTGGGGATGAGTTAAGGTGTCGGGGGCTAACAGcgcg                                                                                                    >  1:469716/1‑140 (MQ=255)

                                         aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTTTGTCG‑GGGTGGGTTGGGGATGGGTTAACTTGTCGTGGAATAAAAGcgcg                                                                                                    >  7:1037548/1‑140 (MQ=255)

                                         aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTATGGCG‑CGGTCGGGTGCGGATGAGTAAAGGTGTCGTGGGATAACAgcggg                                                                                                    >  7:729286/1‑138 (MQ=255)

                                         aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGGGTAGAGTCGGGTTTTGTTCTGGTGGCG‑GGGTGGGTTTCGGAGGTGTATAGGGGTAGTTGGATAACAgcgcc                                                                                                    >  6:1254809/1‑139 (MQ=255)

                                         aCGGCGACGCTGAAACTCTTGCCGGGATATATCACGCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTTCTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGGGGCCAAACcgcgcg                                                                                                    >  8:453084/1‑140 (MQ=255)

                                               aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                                              <  8:377665/140‑1 (MQ=255)

                                               aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGGCGGTTTCGGATGAGTAAAGGGGTGGGGGCATAACAGCGCGGCAacc                                                                                              >  6:1051334/1‑139 (MQ=255)

                                               aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTAATGAG‑CGGTCGGGTGCGGATGTGTAAATGTGTCGTGGGTTTACAGAGCGGCAacc                                                                                              >  5:237894/1‑139 (MQ=255)

                                               aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCGTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTAGTTCTTCTTGGG‑CGGTCGGGGGCCGTGGAGGAAAGGTGTAGTGGCAGAAAAGCGCCGCAacc                                                                                              >  2:745649/1‑139 (MQ=255)

                                                cGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTGTGGCG‑CGGTCGGGTGCGGATGAGTAAAGGTGTCGTGGGATAACAGCGCGGCAac                                                                                               >  4:635484/1‑138 (MQ=255)

                                                  cTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACgg                                                                                           <  2:307368/140‑1 (MQ=255)

                                                   tGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACg                                                                                            <  4:217003/138‑1 (MQ=255)

                                                         tctTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGGGGCATAACAGCGCGGCAACACGGACGTACt                                                                                    <  8:850262/140‑1 (MQ=255)

                                                             gacGGGATATATCACTCATAACAATATCATTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGGGGGGTGGGGTTGAGTTAAGGTGTCGTTGCTTAACAGCGCGGCAACCCGGCCGTACTCg                                                                                  >  4:1123807/3‑138 (MQ=255)

                                                                gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                             <  5:917247/140‑1 (MQ=255)

                                                                gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGGGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                             <  2:865370/140‑1 (MQ=255)

                                                                gggATATATCACTCATAACACTCTACTTACATTTTTTGTTTTTAGTGTAGTGTCGGTTTTTGTTCTTGTGGCG‑CGGGCGGTTGAGGATGAGTAAAGGGGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                             <  5:1126614/140‑1 (MQ=255)

                                                                     atatCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCTAAACAACGCGGCAACCAGGACCTACTCGATGAtctg                                                                          >  4:586570/1‑137 (MQ=255)

                                                                           cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTATTTAACATAAGGCG‑CGGTCGGTTGGGGATGAGATAAGGTGTGGTGGGATTAAAGCGCGGCAACACGGACGGAATCGATGATCTCTTGAATCg                                                                  >  1:872470/1‑140 (MQ=255)

                                                                            tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                                                 <  1:679261/140‑1 (MQ=255)

                                                                             cATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGATTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGAc                                                                <  5:634259/140‑1 (MQ=255)

                                                                                aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTATTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGGAGTGGCATAACAGCGCGGCCACAAGGACGTACTCGATGATCTCATAAAGCGACa                                                               >  4:1147160/1‑138 (MQ=255)

                                                                                 acacTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACAttt                                                            <  2:1186362/140‑1 (MQ=255)

                                                                                     tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCa                                                          <  3:716381/138‑1 (MQ=255)

                                                                                     tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCa                                                          <  4:377898/138‑1 (MQ=255)

                                                                                     tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAg                                                        <  5:1084780/140‑1 (MQ=255)

                                                                                     tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGGTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAg                                                        <  7:453084/140‑1 (MQ=255)

                                                                                     tctcCTTACATTTTTTGTGTTTCGTGTAGAGTCGGTGTTTGTACTTCTGGCG‑CGGTCGGTTGCGGAGGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCa                                                          <  3:130558/138‑1 (MQ=255)

                                                                                      ctcCTTACTTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                       <  7:974164/140‑1 (MQ=255)

                                                                                      ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTTCATGTGGCG‑CGGTCGGGTGGGGATGTGTAAAGGTGTCGTGGCGTAACAACGCGGCCACACGGACGTAGACGCGTATATCTTCAAGCGACATTTCaatc                                                       >  7:363585/1‑138 (MQ=255)

                                                                                      ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTATTATGGCG‑CGGGGGGTTGCGGATGAGTAAAGGTGTCGTGGGATAACACCGCGGCAACACGGCCGGACTCGATGATGTCTTCAAGCGAAAATTCAAGc                                                       >  6:100742/1‑140 (MQ=255)

                                                                                      ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                       <  2:423391/140‑1 (MQ=255)

                                                                                      ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                       <  7:864849/140‑1 (MQ=255)

                                                                                        ccTTTCATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATTACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGctc                                                     <  5:40420/140‑1 (MQ=255)

                                                                                        ccTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTCGTGTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTGGTGGCATAAAAGCGCGGAAACACGGACGGACTCGATGATCTCTTCAAGCGACATTTAAAGc                                                       >  4:981490/1‑138 (MQ=255)

                                                                                            aCATTTTTTGTTTTTAGTGTAGAGGCGGATTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTcc                                                 <  6:952780/140‑1 (MQ=255)

                                                                                                            gtgtAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCATGCTCTTCCTGATcttcgtcttcgt                                 <  8:1072661/140‑1 (MQ=255)

                                                                                                            gtgtAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtcttc                                   <  3:1116804/138‑1 (MQ=255)

                                                                                                            gtgtAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt                                 <  8:1218505/140‑1 (MQ=255)

                                                                                                            gtgtAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt                                 <  5:538929/140‑1 (MQ=255)

                                                                                                                           ttGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  5:446906/140‑1 (MQ=255)

                                                                                                                           ttGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTg                    <  4:821493/138‑1 (MQ=255)

                                                                                                                           ttGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTg                    <  4:4357/138‑1 (MQ=255)

                                                                                                                           ttGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGGGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  2:1237343/140‑1 (MQ=255)

                                                                                                                           ttGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  1:344206/140‑1 (MQ=255)

                                                                                                                           atGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGAGTCGAGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  1:965707/139‑1 (MQ=255)

                                                                                                                             ttACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGcgcg                <  5:1284047/139‑1 (MQ=255)

                                                                                                                             gTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTTcgcg                <  5:347969/140‑1 (MQ=255)

                                                                                                                             gTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGcgcg                <  8:750172/140‑1 (MQ=255)

                                                                                                                              tACTTATGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACAATTAAAGCTCTTCCTGATCATCGTCTTCGTCGGAACCCAACTgcgc                 >  4:964741/1‑138 (MQ=255)

                                                                                                                                cTTCTGGCG‑CGGTCGGTGGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATg             <  5:706207/140‑1 (MQ=255)

                                                                                                                                  tCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGtt           >  6:587749/1‑140 (MQ=255)

                                                                                                                                  tCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGtt           >  6:399623/1‑140 (MQ=255)

                                                                                                                                  tCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGtt           >  2:1121379/1‑140 (MQ=255)

                                                                                                                                    tGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGGCATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGtt           >  4:505069/1‑138 (MQ=255)

                                                                                                                                    tGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGtt           >  4:1353107/1‑138 (MQ=255)

                                                                                                                                       cg‑cgGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACg        <  3:1253622/138‑1 (MQ=255)

                                                                                                                                        g‑cgGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGAGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGATACCCAACTGCGCGATGTTCCGc       >  3:17457/1‑138 (MQ=255)

                                                                                                                                          cggtcggtTGCGGATGAGTATAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGa    <  2:591313/140‑1 (MQ=255)

                                                                                                                                          cggtcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGa    <  1:631287/140‑1 (MQ=255)

                                                                                                                                          cggtcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGa    <  6:625269/140‑1 (MQ=255)

                                                                                                                                            gtcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGAtc  >  6:803291/1‑140 (MQ=255)

                                                                                                                                            gtcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGAtc  >  2:66373/1‑140 (MQ=255)

AGCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCG‑CGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATC  >  NZ_CP022686/1674104‑1674382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A