Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F13 I0 R1 235 6960.4 9135780 98.4% 8989607 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,674,244 T→G 15.4% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,674,2440TG15.4% 278.1 / 13.1 178T182P (ACC→CCC) ygfBUPF0149 family protein YgfB
Reads supporting (aligned to +/- strand):  ref base T (37/108);  new base G (24/8);  total (62/116)
Fisher's exact test for biased strand distribution p-value = 2.55e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

GCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATC  >  NZ_CP022686/1674105‑1674382
                                                                                                                                            |                                                                                                                                           
gcgcGGCGCTGCCGTGTTGCATTTGATCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCTTAACACTCTCCTTACATTTTTTGTTTTTAGTGTATAGTCGG‑TTTTTGTACTTCTGGCGcggt                                                                                                                                             <  7:1072530/140‑1 (MQ=255)
gcgcGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATTTATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGcggt                                                                                                                                             <  6:903621/140‑1 (MQ=255)
gcgcGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGcggt                                                                                                                                             <  6:345267/140‑1 (MQ=255)
     gcgcTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTTTACGTCTGGCgcgggcgggt                                                                                                                                        >  8:939569/1‑138 (MQ=255)
      cgcTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTg                                                                                                                                       <  6:35553/140‑1 (MQ=255)
      cgcTGCCGGGTTGCATTAGCTCTACCAGGTCCTGCCGGCGACGCTGAAACTCTTGCCGGTATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGcggtcggt                                                                                                                                         <  3:439128/138‑1 (MQ=255)
         tGCCGGGTTTCAGTTGCTCTACCCGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGTTATATCACTCATAACACTCTCATTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                    <  8:449642/140‑1 (MQ=255)
         tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGc                                                                                                                                      <  3:855894/138‑1 (MQ=255)
         tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                    <  2:956244/140‑1 (MQ=255)
         tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                    <  6:1095554/140‑1 (MQ=255)
         tGCCGGGTTGCATTTGCGCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACCTTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                    <  8:427632/140‑1 (MQ=255)
         ggCCGGGTTGCAGTTGCTCTACCACGGCCTGACGGCGACGCTGAAACGCTTGCCGGGATATCTCACTCATAACACTCTCCTTCCATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                    <  6:655157/139‑1 (MQ=255)
           ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                    <  4:176259/138‑1 (MQ=255)
           ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                    <  3:1126485/138‑1 (MQ=255)
           ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGAt                                                                                                                                  <  7:209445/140‑1 (MQ=255)
                  gCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTGTA‑TTATGGGGCGGTcgggtgcgggtgcggt                                                                                                                             >  3:953315/1‑134 (MQ=255)
                       tGCTCTACCAGGGCCTGACTGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                      <  8:1040559/140‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                      <  7:819985/140‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                      <  5:250007/140‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                      <  2:599845/140‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgt                                                                                                                        <  3:351802/138‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGAGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                      <  1:584500/140‑1 (MQ=255)
                       tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TATTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                      <  8:640107/140‑1 (MQ=255)
                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                    <  5:391095/140‑1 (MQ=255)
                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                    <  1:870646/140‑1 (MQ=255)
                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                    <  1:738479/140‑1 (MQ=255)
                         ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                    <  2:214875/140‑1 (MQ=255)
                          tctACCAGGGCCTGACGCCGACGCTGAAACTCTAGCCGGGATATATCACTCATAACACTCTCCTTACTTTTTATGTTTTGAGTGTAGAGTCGG‑TTTTTGTAAGTGAGGCGCGGTCGGGTGGGGATGAGTAAAGGTGTCgg                                                                                                                   >  7:523587/1‑139 (MQ=255)
                            tACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTgg                                                                                                                 <  7:857416/140‑1 (MQ=255)
                              ccAGGGCCTGACGGAGACGCTGAAACTCTTGCCTGGATATATCAATCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTTTACTTCTGGAGCGTTGGTGTGGGGATTAGTAAAGATGTAGTggga                                                                                                               >  8:279864/1‑138 (MQ=255)
                               cAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCAt                                                                                                              <  6:507900/140‑1 (MQ=255)
                                  ggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGGTCTTCTGGGTCGGGAGGTTGGGGATGAGTTAATGGTTTGTGGCAAAAc                                                                                                           >  1:515977/1‑140 (MQ=255)
                                    ccTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCTG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAAc                                                                                                           <  3:652239/138‑1 (MQ=255)
                                    ccTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GGTTTGTACTTCTGGCGCGGGGCGTTGCGGGTGAGTTAAGGTGTGGTGGGAGAAc                                                                                                           >  3:1048691/1‑138 (MQ=255)
                                         cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTTGCGCGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAgcgc                                                                                                      <  3:271716/138‑1 (MQ=255)
                                         cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCgg                                                                                                    <  2:500557/140‑1 (MQ=255)
                                         cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCATTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAATGTGTCGTGGCATAACAgcgc                                                                                                      <  4:378980/138‑1 (MQ=255)
                                              aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                                               <  8:501566/140‑1 (MQ=255)
                                               cGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGGTGGGGATGAGTAAACGGGTCGGGGCATAACCGCGCGGCAac                                                                                                >  4:258046/1‑138 (MQ=255)
                                                 cTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACTTTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑Cgg                                                                                           <  2:679320/140‑1 (MQ=255)
                                                    aaaCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACg                                                                                        <  5:1013518/140‑1 (MQ=255)
                                                          ttGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACt                                                                                    <  3:974948/138‑1 (MQ=255)
                                                           tGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTAAATATGGGGCGGTGGGTTGGGGAAGAGTAAATGTGTGGTGGCGTAAAAACCCGGCAAAA‑CCGACggaatcca                                                                                 >  5:552285/1‑133 (MQ=255)
                                                               gtgATATATCACTCATAACACTCTCCTTACTTTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTTGCGCGTTCGGTTGCGGATGAGTAAATGAGTCGTGGCATAACTGCTCGGCTACT‑CTGACGTACTCgatg                                                                               <  4:509893/136‑1 (MQ=255)
                                                               gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCTGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCgatgat                                                                             <  6:212307/140‑1 (MQ=255)
                                                               gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCgatgat                                                                             <  7:250994/140‑1 (MQ=255)
                                                               gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCgatgat                                                                             <  5:961567/140‑1 (MQ=255)
                                                                        cACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTAATTCTGGCGCGGTCGGTTGGGGATGAGTAAAGGTGTAGTGGCATAACAGCGCGGCCACA‑CGGACGTACTCGATGATCTCttga                                                                      >  4:418839/1‑136 (MQ=255)
                                                                          cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTTTTCATCTTGCGCGGTCGGTTTCGGATTATTAAAAGTGTAGTGGCATAAAACCGAGGCCACA‑CCGACGGACACGATGATATCATCAAGcc                                                                  >  5:91051/1‑139 (MQ=255)
                                                                          cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTTTAATTAATGAGCGGGAGGTTGCGGATGAGTAAAAGTGGAGTGGCAGTCCTGGGCGGCAACA‑CGGCAGTACTGGAGGGACTCTGCaacgg                                                                  >  8:1069237/1‑137 (MQ=255)
                                                                          cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTTTACTTCTGGCGCGGGCGGTTGGGGATGAGTTAAGGTGTGGTGGCATAACAGCGCGGCTACA‑CGGCCGTACGCGATGATATCTTAAATCg                                                                  >  8:149432/1‑140 (MQ=255)
                                                                           tCATAACCCGCTGCTTATATTTTTTGTTTTTAGTGTAGAGGCTG‑TTTTTGTCCTTCTGGCGCGGTCGGTTGCGGATGAGTATAGGGGTCGTGGCATAACAGCGAGGGAACA‑CGGAAGTACTCGCTGAGCTCTTCAAGCGa                                                                 <  2:104482/140‑1 (MQ=255)
                                                                           tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCTGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATTATCTCTTCAAGCGa                                                                 <  8:70826/140‑1 (MQ=255)
                                                                           tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTCTGGCGCGGGCGGTTGCGGATGAGTAAAGGGGTCGTGGGCAAACAGCGCGGGAACA‑CGGCCGTCCTCGAGTATCTCAGAAAGCGa                                                                 >  6:765716/1‑140 (MQ=255)
                                                                            cATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCg                                                                  <  4:1153678/138‑1 (MQ=255)
                                                                            cATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGAc                                                                <  1:266276/140‑1 (MQ=255)
                                                                            cATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTAAATAGGGCGCGGTAGGGTGGGGATGATTAAATTGGTCGTGGGATAACAGGGCGGCCACA‑CGGACGTACTCGAAGATCTAATCAAGCg                                                                  >  4:306934/1‑138 (MQ=255)
                                                                             atcacacTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGCGG‑GTTTTGGACTTCTGGCGCGGGGGGTTGCGGGTGAGTATAGGTGTCGTGTCATAACAGCGCGGCCACA‑CGGCACTGCTCGAGAATCTCTTCAAGCGACa                                                               >  8:76084/4‑140 (MQ=255)
                                                                             aTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTTTAAATCTGGGGCGGGCGGGTGCGGATGAGTAAAAGTGTCGGGGCCTAACAGCGCGGCAACA‑CGGACCTACAAGATTCTCTCATCAAGCgccc                                                               >  1:362029/1‑137 (MQ=255)
                                                                             aTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTTCTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGGATAACCGCGCGGCAACA‑CGGCCGTTCTCGATTCTCTATTCAAGCgc                                                                 >  4:949616/1‑137 (MQ=255)
                                                                             aTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTCTGGCGCGGTGGGGTGCGGATGAGTTAAGGTGGAGTGGCATAACAGCGCGGCAACC‑CGGACGTACTCGATGATCTCGTCAAGCgg                                                                 >  4:672149/1‑137 (MQ=255)
                                                                               aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTTTACTTCTGGCGCGGGCGGGGGCGGCTGAGGTAAGGGGTCGGGGCATAACAGCGCGGCCACA‑CGGGCGTACACGATGCTCTCTTCAAGCGACaat                                                             >  7:695968/1‑138 (MQ=255)
                                                                               aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTTCTTATTGGCCGGTGCGGTGCGGATGTGTAAACGTGGAGGGGCATAACAGCGCGGGCACACCGCACG‑ACTCGATGATATATTAAACCGACAtt                                                             >  6:137006/1‑140 (MQ=255)
                                                                                acacTCTCCTTAGATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACAttt                                                            <  1:198329/140‑1 (MQ=255)
                                                                                    tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTAAGTCTGGCGCGGGGGGGTGCGGATGAGGAAAGGTGTGGGGGGATAACAGCGCGGCAACA‑CGGCCGTACTCGATGATATCTTAAATCGACATTTCaac                                                        >  2:1055806/1‑139 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTTTGCTTAGTGCGCGGTCGGGTGCGGCTGATTAAATGGGTAGTGGCGTAAACGCGCGGCCACA‑CCGACGTACACGCTGATCTCTTCAAGCGACATTTAAAGc                                                       >  7:62612/1‑140 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                       <  5:340867/140‑1 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                       <  1:903186/140‑1 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACATCTGTCGCGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCAAAACAGCGCGGCCACA‑CGGCCGGACTCGATGCACTCTTCACGCGACCTTTaaaag                                                       >  8:881923/1‑138 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTTTACTTATGGCGCGGGAGGTTGGGGATGAGTAAAGGTGTCGTGGCCTAAAACCGCGGCCACA‑CGCCCGTACTAGATGATATATGCAAGCGCCAATTaaacc                                                       >  1:494885/1‑138 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTCTGGCGCGGTGGGTTGGGGATGAGGTAACGTGTCGTGGCATAACAGCGCGGCAACA‑CGGCCGTTCTCGAGGATCTCTTCAAGCGACAGTTCAAGc                                                       >  7:271405/1‑140 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTCTGGCGCGGGGGGGTGCGGATGTGTAAAGGTGTCGGGGCATAACAGCGCGGCCAAA‑CGGACGTCCTAGATGATGTCTTAAAGCGACCTTTCAAGc                                                       >  7:807477/1‑140 (MQ=255)
                                                                                     ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTAATTCTGGGGCGGTGCGGTGCGGATTGGTAAAGCTGTCGGGGCATAACAGCGCGGCCACA‑CCGACGTAATCGATGATATCTTCAAGCGACAATTCaatc                                                       >  8:753988/1‑138 (MQ=255)
                                                                                     ctcCTTACATTTGTTGTTTTTAGGGGAGAGTCGG‑TTTTTGTACTGCTGGCGCGGTCGGTTGCGGATGAGGAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                       <  7:978998/140‑1 (MQ=255)
                                                                                       ccTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTAACTCAGGCGCCGGCGGTTGGGGCAGAGTAAAGGTTGCGTGGCATAACACCGCGGCAACC‑CCGTCCTTCTCGATGAACTCTTAAAGCGCCAATTCAAGc                                                       >  4:230775/1‑138 (MQ=255)
                                                                                           aCATTTTTTGTTTTTAGTGTCGAGTCTG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTACAGGTGTCGTGGCATATCAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTcc                                                 <  8:321604/140‑1 (MQ=255)
                                                                                           aCATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTcc                                                 <  8:398010/140‑1 (MQ=255)
                                                                                           aCATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCGCTTCAAGCGACCTTTCAAGctctg                                                   >  4:898268/1‑137 (MQ=255)
                                                                                             aTTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTg                                               <  6:552285/140‑1 (MQ=255)
                                                                                                ttttGTTTTTAGTGTAGAGTCGG‑TTTTTTTACTTCTGGCGCGGTCGGTTGGGGATGAGTAAAGGGGTGGTGGCATAACAGCGCGGCAACA‑CGGAAGTACTCGATGAAATATTAAACAGACATTTAAATCTCTTCCTGAta                                            >  6:365168/1‑139 (MQ=255)
                                                                                                ttttGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtc                                            <  5:139251/140‑1 (MQ=255)
                                                                                                ttttGTTTTTAGTGTAGAGTCGG‑TCTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTTAAGGTGTCGTGGCATAACAGAGCTGCCACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAATATCTTCCTGAta                                            >  2:742043/1‑139 (MQ=255)
                                                                                                  ttGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTCTGGCGCGGTCGGGTGCGGATGAGTAAAGGTGTCGGGGGATAACAGCGCGGGAACC‑CGGACGTACTAGCTGATCGCTTCAAGCGCCCTTTAAAGCGCTTCCTGAtcat                                          >  2:15824/1‑138 (MQ=255)
                                                                                                       tttAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtct                                     <  1:1020408/140‑1 (MQ=255)
                                                                                                       tttAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGGTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgt                                       <  3:901519/138‑1 (MQ=255)
                                                                                                                     gg‑TTTTTGTACTTCTTGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACACCGCGGCAACA‑CGGACGTACTCGATGATATCTTCAAGCGACATTTAAAGCTCTTCCATATCTTCGTCTTCGTCGTAACCCaa                       >  8:847490/1‑140 (MQ=255)
                                                                                                                     gg‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCaa                       <  5:1032331/140‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  1:145489/140‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTg                    <  3:209179/138‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTAGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTg                    <  4:984341/138‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGGGGCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCGCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCACCTg                    <  4:980080/138‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                  <  7:76106/140‑1 (MQ=255)
                                                                                                                           ttGTACTTCTGGCGCGGGAGGTTGCGGAGGCGTAAAGGGGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGGTCTTCGTCTTCGTCGTAACCCAACTgcg                  <  8:254662/140‑1 (MQ=255)
                                                                                                                               aCTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCTGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTTATCTTCGTCTTCGTCGTAACCCAACTTCGCGAt              <  7:1055838/140‑1 (MQ=255)
                                                                                                                                     ggCGCGGGCGGTTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCGCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACg        <  6:288991/140‑1 (MQ=255)
                                                                                                                                      gcgcGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATTATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGc       <  7:334895/140‑1 (MQ=255)
                                                                                                                                       cgcgGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCa      <  2:267050/140‑1 (MQ=255)
                                                                                                                                         cggtcggcTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGa    <  1:227775/140‑1 (MQ=255)
                                                                                                                                         cgggcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGa    <  2:550613/140‑1 (MQ=255)
                                                                                                                                           gtcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGAtc  >  6:92317/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                           
GCGCGGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACA‑CGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATC  >  NZ_CP022686/1674105‑1674382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A