Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A29 F36 I0 R1 179 9550.8 8038540 98.3% 7901884 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,130,829 A→C 16.7% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,130,8290AC16.7% 261.5 / 16.7 160V237G (GTT→GGT) mreCrod shape‑determining protein MreC
Reads supporting (aligned to +/- strand):  ref base A (101/28);  new base C (9/21);  total (111/49)
Fisher's exact test for biased strand distribution p-value = 9.12e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

TTCCGGCGTCATCGGGTTAGCGCCGTTACGATCCGCGCCCCACAGCAGCAGCAGATAACGCAAACGTTGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGC  >  NZ_CP022686/2130690‑2130967
                                                                                                                                             |                                                                                                                                          
ttCCGGCGTCATCGGGTTAGCGCCGTTACGATCCGCGCCCCACAGCAGCAGCAGATAACGCAAACGTTGCA‑G‑TCCTGCAGTCGGACGCGCCTGACTCACAGTATAAGCGCTCTGGGTATCGAGAGTTACGGAAGAGACaa                                                                                                                                            >  1:434595/1‑140 (MQ=255)
 tCCGGCGTCTTGGGGTTAGGGCGTTTACGAGCCGCGCCCCACAGCAGCAGCATATAACGAAAACGTTGCA‑G‑TCCTGCAGTGGGACGCGCCTGAATCACTTTATATGCGCGCTGGGTATCGTTTTTTACGGATGAGACAAc                                                                                                                                           <  4:523040/140‑1 (MQ=255)
 cccGGCGTCATCGGGTTAGCGCAGTTACGTGCGGCCCCACCCTGCATCTGCAGTTAACGCAAATGTTGAT‑G‑TCCTGCAGTCGTAGGCGCGTGTTTCACTGTATAAGGGCGGTGGTTATCGAGTTTTAGTTAAGACACAAc                                                                                                                                           <  6:680384/139‑1 (MQ=255)
  ccGGCGTCATCGGGTTAGCGCCGTTACGATCCGCGCCCCACAGCAGCAGCAGATAACGCAAACGTTGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAAcc                                                                                                                                          >  1:826632/1‑140 (MQ=255)
     gCGTCATCGGGTTAGCGCCGTTACGATCCGCGCCCCACAGCAGCAGCAGATAACGCAAACGTTGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACcgcg                                                                                                                                       >  2:745435/1‑140 (MQ=255)
          aTCGGGTTAGCGCCGTTACGATCCGCGCCCCACAGCAGCAGCAGATAACGCAAACGTTGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCgg                                                                                                                                  >  3:134110/1‑140 (MQ=255)
          aTCGGGTTAGCGCCGTTACGATCCGCGCCCCACAGCAGCAGCAGATAACGCAAACGTTGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTAAGGAAGAGACAACCGCGACCgg                                                                                                                                  >  3:947506/1‑140 (MQ=255)
          aTCGGGTTAGCGCCGTTACGATCCGCGCCCCACAGC‑‑C‑GCAGATAACGCAAACGTTGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTGTTACGGAAGAGACACCCGCGACCGGATa                                                                                                                               >  2:859150/1‑140 (MQ=255)
          aTCGGGTTAGCGCCGTTACGATCCGCGCCCCA‑‑‑CAGCAGCAGATAACGCAAACGTTGCA‑G‑TCCTGCAGTCGGCCGCGCCTGAATCACAGGATAAGCGCGCTGGGGATCGAGTTTTACGGAAGAGACAACCGCGACCGGATa                                                                                                                               >  6:371289/1‑140 (MQ=255)
            ggggTTAGCGCCGTACCGATCCGCGCCCCACCGCAGCAGCAGATAACGCAACCGTTGCT‑C‑TCCTGCAGTCGGACGCGCCGGAATCACAGTATACGCGCGCGGGGTATCGAGTTTTACTCCAGAGACACCCGCGACCGGAt                                                                                                                                <  6:954616/139‑1 (MQ=255)
               tttAGCGCCGTTACGATCCGCGCCCCACAGCAGCAGCATATAACGCAAACGTTGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGGGCTGGGTATCGAGTTTTACGGAAGATACAACCGCGACCGGATAGc                                                                                                                             <  2:4066/139‑1 (MQ=255)
                  aGCGCCGTTACGCTCAGCGCCCCACCGCAGCAGCAGATAACGCAAACGTTGCA‑G‑TCCTGCATTCGGACGCGCCTGAATCCCAGTATAAGCTCGCTGGCTATCGAGTTTTACGGAAGCGCCAGCCGCGACCGGCTCGCCtt                                                                                                                          >  6:100673/1‑140 (MQ=255)
                  aGCGCCGTTACGATCCGCGCCCCACAGCAGCAGCAGATAACGCAAACGTTGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGCCAACCGCGACCGGATCGCCtt                                                                                                                          >  5:499426/1‑140 (MQ=255)
                  aGCGCCGTTACGATCCGCGCCCCACAGCAGCAGCAGATAACGCAAACGTTGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCtt                                                                                                                          >  4:281122/1‑140 (MQ=255)
                         ttACGATCCGCGCCCCACAGCAGCAGCAGATATCGCAAACGTTGCC‑G‑TCCTGCAGTCGGACGCGCCTGAATCAAAGGATAAGCGCGCTGGGTAGCGAGTTTTACGGAAGAGTCAACCGCGACCGGATAGCCTTCCGGGaa                                                                                                                   >  7:285255/1‑140 (MQ=255)
                         ttACGATCCGCGCCCCACAGCAGCAGCAGATAACGCAAACGTTGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACACCCGCGACCGGATAGCCTTCCGGGaa                                                                                                                   >  6:407609/1‑140 (MQ=255)
                         ttACGATCCGCGACCCACAGCAGCAGCAGATAACGCAAACGTTGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCCCAGTATAAGCGCGCTGGGTTTCGAGTTTTACGGAAGAGACAACCGCGGCCGGATAGCCTTCCGGGaa                                                                                                                   >  2:778064/1‑140 (MQ=255)
                              aTCCGCGCCCCACAGCAGCAGCAGATAACGCAAACGTTGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGAc                                                                                                              >  1:2860/1‑140 (MQ=255)
                              aTCCGCGCCCCACAGCAGCAGCAGATAACGCAAACGTTGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGAc                                                                                                              >  4:36018/1‑140 (MQ=255)
                                  cccccccACAGAAGCAGCATATAACGAAAACGTTGCA‑G‑TCCTCCAGTCGCACGCCCCTCAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGcc                                                                                                          <  8:704128/137‑1 (MQ=255)
                                   cgcCCCACAGCAGCAGCAGATAACGCAAACGTTGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGccc                                                                                                         >  3:75501/1‑140 (MQ=255)
                                          cagcagcagcatAAAACGCAACCGTTGCA‑G‑TCCTGCAGGCGGCCGCGCCTGAATCACCTAATAAGCGCGCTGGGTACCGAGTTTTCCGTAAGACACACCCGCGACCTTATATAATTCCGGGAAACGACCGCCCAgtagtg                                                                                                  <  4:846801/140‑6 (MQ=255)
                                            gcagctgcagATACCGCAAA‑GTTTCATG‑TCTTGCTGTGGGACGCGCCTTAATCACATTAAACGCGGGCTGGGTATAGAGTTTTACGGAATATAAACCCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGaa                                                                                                <  3:777725/140‑1 (MQ=255)
                                            ccagcaccatATACCGCAAACGTTGCA‑G‑TCCTGCCGTCGGACGCGCCTCAATCACAGTAAACGCGCGCTGGGTAGCGAGTTTTACGCAAGAGACACCCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGaa                                                                                                <  8:560080/139‑1 (MQ=255)
                                              agcagaatATAACGAAAACGTTGCA‑T‑TCCTGCAGTCGCACGCGCCTGAATCACAGTATACGCGCGCTGGGTATCGAGTTTTACGGAATAGACACCCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGt                                                                                              <  2:82998/140‑1 (MQ=255)
                                                 ggaataTAACGCAAA‑GTTGGA‑GTTCGGGCAGGCGGCCGCGCCTGAATCACTGTATACGCGCGCTGGTTCGCGGGTTTTACGGAACTGAAACCCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCAc                                                                                           <  8:746585/135‑1 (MQ=255)
                                                 agcttaTAACGCAAAGTTTGCC‑G‑CCCTGCAGTCGGACGCGCCTTAATCACAGTATAAGGGCGCTGGGTATCGCTTTTTCCGGAAGAGAAAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCAc                                                                                           <  8:59739/135‑1 (MQ=255)
                                                  gcagATAACGCAAACGTTGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACACCCGCGACCGGATAGCCTTCCGGGAACCGCCCGCCCAGACCGGAAGTCAcc                                                                                          >  1:625031/1‑140 (MQ=255)
                                                  gcagATAACGCAAACGTTGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAACCGACCGCCCAGACCGGAAGTCAcc                                                                                          >  5:93189/1‑140 (MQ=255)
                                                  ccataTAACGCAAACGTTGCA‑G‑ACCTGCAGTCGGACGCGCCTGAATCACTGTATAAGCGCGCTGGGTATCGAGTTTTACGTAAGAGACACCCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCAcc                                                                                          <  2:589100/136‑1 (MQ=255)
                                                      aaaccGCAAACGTTCCC‑G‑CCCTCCAGGCGCCCGCGCCTCAAACACATTATACGCGCGCGGGGTATCGTTTTTACCGTACGACACAACCGCGACCGTATAGCCTTCCGTGAAAAGACCGCCCAGACCGGAAGTCACCAgat                                                                                      <  6:124821/136‑3 (MQ=255)
                                                      aTAACGAAAACGTTGCA‑G‑TCCTGCAGTGGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTAGCGTTTTTTACGGAAGACACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGca                                                                                      <  8:507081/140‑1 (MQ=255)
                                                       aaaCGCAACCGTGGCA‑G‑GCCTGCTGGCGGACGCGCCGCAATCACATTATACGCGCGCGGGGTATCGTGTTTTCCGGACGAGACACCCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGcac                                                                                     <  4:497254/139‑1 (MQ=255)
                                                        aaCGCAAACGTTGCT‑G‑GCCTGCTGTGGTTCGCGCCTTAATCATATTATAAGGGGGGTGGGTATCTTTTTTTCCGTATTACACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGcaca                                                                                    <  3:926707/140‑1 (MQ=255)
                                                               acattGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCaa                                                                             >  7:397746/4‑140 (MQ=255)
                                                               aCGTTGCT‑G‑ACAGGCAGTCGCCCGCGCTGTAATCACCGTATATGCGCGCTGGGAATCGAGTTTTCCGGATCAGACACCCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCaa                                                                             <  7:886954/140‑1 (MQ=255)
                                                               aCGTTGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCaa                                                                             >  7:534717/1‑140 (MQ=255)
                                                                  ttGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAacac                                                                          >  2:923271/1‑140 (MQ=255)
                                                                     tgccgcccTGCTGTGGGACGCGCCTCAAACACAGTATAAGCGCGCTGGGTCTCGCGTTTAACGGACCAGACACCCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACg                                                                         <  4:346287/134‑1 (MQ=255)
                                                                             tGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACACCCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCg                                                                 >  7:916040/1‑140 (MQ=255)
                                                                             tGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGCCCGGAAGTCACCAGCACATCACCACCACGAATATCCg                                                                 >  5:68771/1‑140 (MQ=255)
                                                                             tGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCg                                                                 >  5:347459/1‑140 (MQ=255)
                                                                               cAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTa                                                               >  4:145699/1‑140 (MQ=255)
                                                                                    tcaCGCGCCTGAATCACAGTATAAGAGCTCTTGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGGCACCAGCACCTCACCAACACGAATATCCGTATTCGc                                                          >  6:1371/3‑140 (MQ=255)
                                                                                         cgcCTTAATCACTGTATACGCGCGCGGGTTATCTATTTTTCCGTACTAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCa                                                     <  6:850328/140‑1 (MQ=255)
                                                                                         cgcCTTAATCACAGTATAAGCGCGCTGGGTATCGTGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCa                                                     <  8:388053/140‑1 (MQ=255)
                                                                                         cgcCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCa                                                     >  2:339670/1‑140 (MQ=255)
                                                                                            cTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGAt                                                  >  2:479827/1‑140 (MQ=255)
                                                                                                    ccgTATACGCGGGCGGGGTTGCGAGTTTTCCGGACGCGCCCCCCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGc                                          <  7:379829/138‑1 (MQ=255)
                                                                                                    caGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACACCCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGc                                          <  7:568756/140‑1 (MQ=255)
                                                                                                    caGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCAGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACAACACCAACACGAATATCCGTATTCGCTGGCAGATGCTCaatc                                          >  6:61926/1‑138 (MQ=255)
                                                                                                      gTATAAGCGCGCTGGGTATCGAGTTTGACGGAAGAGACAACCGCGCCCGGATAGCCTACCGGGAACCGACCGCCCAGACCGGAAGTCACCAGCACAACACCAACACGAATATCCGTATTCGCTGGCAGCTGCTCAAGCTg                                        >  8:134795/1‑140 (MQ=255)
                                                                                                         taAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACACCCGCGACCGGATAGCCTTCCGGGCAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCaa                                     >  4:1003154/1‑140 (MQ=255)
                                                                                                         taAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCaa                                     >  4:752759/1‑140 (MQ=255)
                                                                                                            gcgcgcTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACTAATATCCGTATTAGCTGGCAGATGCTCAAGCTGCAAatc                                  >  3:357097/1‑140 (MQ=255)
                                                                                                            gcgcgcTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAatc                                  >  1:118596/1‑140 (MQ=255)
                                                                                                            ccgcgcTGGGTATCGAGTCTTACGGACGAGAAAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCACCACATCACCAACACGAATATCCGTATTGGCTGGCAGATGCTCAAGCTGCAAatc                                  <  6:90007/139‑1 (MQ=255)
                                                                                                               cgcTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGCCCGCCCAGACCGGAAGTCACCAGCACATCACCAACCCGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAatcatc                               >  1:860636/1‑140 (MQ=255)
                                                                                                               cgcTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACCTCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAatcatc                               >  5:591808/1‑140 (MQ=255)
                                                                                                               cgcTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAatcatc                               >  4:707653/1‑140 (MQ=255)
                                                                                                               cgcTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAatcatc                               >  7:73739/1‑140 (MQ=255)
                                                                                                               cgcTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATAACCGTATTCGCTGGCAGATGCTCAAGCTGCAAatcatc                               >  4:875975/1‑140 (MQ=255)
                                                                                                               cgcTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCATCCGGGAAACGACCGCCCAGCCCGGCAGTCACCAGCACATCACCATCACGAATATCCGTATTCGCTGGCAGATGAGCAAGCTGCAAatcatc                               >  3:902287/1‑140 (MQ=255)
                                                                                                               cgcTGGGTATCGAGTTTCACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGCCCGCCCAGACCGGAAGTCAACAGCACATCAACAACACGAATATCCGTATTCGCTGGCAGATTCTACAGCTGAAAatcatc                               >  2:964531/1‑140 (MQ=255)
                                                                                                                gcTGGGTATCGAGTTTTACGGAAGCGACAACCGCGCCCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATcc                              >  1:228256/1‑140 (MQ=255)
                                                                                                                 cTGTGTATAGAGTTTTACGTAAGAGACAACCTCGACATGATAGCCTTCCGGGAAACGACCGACCAGAACGGAAGTCACAATCAAATCACAAACACTAATATACGTATTCGATGAAACATGATAAAGATACAAatcaacat                             >  4:343581/1‑136 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTACTGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATACTTATACGCTGGCAGATCCTCAAGCTGCAAATCATCCg                             >  7:727145/1‑140 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTACGGAAGAGACAACCGCGCCCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCg                             >  3:565878/1‑140 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGCTAGCCTTCCTGGAACCGACCGCCCAGACCGGAAGTCACCAGCCCATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAACTCATCCg                             >  7:800385/1‑140 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGCCCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCg                             >  3:55377/1‑140 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGCCCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCg                             >  6:432903/1‑140 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGCTGCTCAAGCTGCAAATCATCCg                             >  3:943820/1‑140 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCg                             >  1:344287/1‑140 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCg                             >  2:279576/1‑140 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCg                             >  3:643075/1‑140 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCg                             >  4:155134/1‑140 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCg                             >  3:832005/1‑140 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCg                             >  1:113969/1‑140 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCg                             >  4:679945/1‑140 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCg                             >  1:432594/1‑140 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCg                             >  1:967837/1‑140 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCg                             >  6:24763/1‑140 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACAGCCCAGACCGGAAGTCTCCAGCACATCACCAACACGAATATCAGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCg                             >  6:432906/1‑140 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAAAGCCCGCCCAGACCGGAAGTCACCAGCACATAACCTACACGAATATCCGTATTCTCTGGCAGATGCTCAAGCTGCAAATCATCCg                             >  7:320379/1‑140 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTACGGAAGAGAAACCCGCGACCGGAAAGCATTCAGGGAAACGACCGCCCAGAACGGAAGTCACCAGCACCTCACCAACACGAATCTCCGTATTCGCTGGCAGATGCTCAAGCTGAAAATCATCCg                             >  7:252067/1‑140 (MQ=255)
                                                                                                                 cTGGGTATCGAGTTTTAAGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCTCCCAGACCGGAAGTCACCAGCACATAACAAACAAGAATATCCGTATTCGCTGTCAGAAGCTCAAGCTGCAAatcatcag                             >  8:130334/1‑138 (MQ=255)
                                                                                                                   gggTTTCGAGTTTAACGGACGACACACCCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCGTa                           <  1:5822/140‑1 (MQ=255)
                                                                                                                   gggTATCGTTTTTTAGGTAATCGACACCCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCGTa                           <  3:336555/140‑1 (MQ=255)
                                                                                                                   gggTATAGTGGTTTACGGAATACACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCGTa                           <  4:918852/140‑1 (MQ=255)
                                                                                                                    ggTTTCGAGTTTTCCGGAACTCAAACCCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCGTAc                          <  6:886880/140‑1 (MQ=255)
                                                                                                                    ggTATCGTGTTTTACGTAAGACACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCGTAc                          <  1:527437/140‑1 (MQ=255)
                                                                                                                      tagagtgTTTAACGCAACAGACACCCGCGACCGGATAGCCATCCGGGAAACGACCTCTCAGACCGGAATTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCTAATCATACGTACaa                        <  4:224347/134‑1 (MQ=255)
                                                                                                                          gAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCAGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGt                    >  2:477639/1‑140 (MQ=255)
                                                                                                                              tttACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTAcc                >  2:976879/1‑140 (MQ=255)
                                                                                                                                tACGGAAGAGACAACCTCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTCTTCGCTGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTGACCgg              >  4:575770/1‑140 (MQ=255)
                                                                                                                                tACGGAAGAGACAACCGCTACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATACGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCgg              >  6:358649/1‑140 (MQ=255)
                                                                                                                                       gagaCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAAt       >  4:283854/1‑140 (MQ=255)
                                                                                                                                         caCAACCGCGACCTGATAGCCTTCCGGGAAACGACCGCCCAGACCTGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTa     <  8:797171/139‑1 (MQ=255)
                                                                                                                                          aCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACAAACACGAATATCCGTAATCGCTGGCAGATGCTCAAGCAGCAAATCATCCGTACAACCGTTACCGGATGCAATTAc    >  5:776370/1‑140 (MQ=255)
                                                                                                                                            aaCCGCGACCGGATAGCCTTCCGGGAACCGACCGCCCAGCCCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCACGCTGCAAATCATCCGTACAACCGTTACCGGCGGCAATTAcgc  >  7:540527/1‑140 (MQ=255)
                                                                                                                                             |                                                                                                                                          
TTCCGGCGTCATCGGGTTAGCGCCGTTACGATCCGCGCCCCACAGCAGCAGCAGATAACGCAAACGTTGCA‑G‑TCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCTGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGC  >  NZ_CP022686/2130690‑2130967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A