breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 46 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,738,4480AC70.8% ‑5.8 / 15.5 24V84G (GTG→GGG) trmDtRNA (guanosine(37)‑N1)‑methyltransferase TrmD
*NZ_CP0092732,334,2420TC62.5% ‑0.6 / 11.6 16Q1136R (CAG→CGG) yfaLAIDA‑I family autotransporter adhesin YfaL/EhaC
*NZ_CP0092733,032,3980AC62.5% 8.9 / 19.4 24G235G (GGT→GGGxerDsite‑specific tyrosine recombinase XerD
*NZ_CP009273244,9690AC61.5% 5.2 / 10.6 13pseudogene (1589/1713 nt)BW25113_RS01160flagellar biosynthesis protein FlhA
*NZ_CP0092731,906,0110TG61.1% 9.9 / 16.9 18T275P (ACC→CCC) htpXprotease HtpX
*NZ_CP0092734,594,1610CT60.6% ‑3.3 / 32.1 33S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP0092734,594,1670CT60.6% ‑3.0 / 35.9 33S64F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP0092731,118,8570TG60.0% 2.7 / 11.8 15intergenic (‑128/+6)yceB/grxBlipoprotein/glutaredoxin 2
*NZ_CP00927356,3790TG59.5% 8.2 / 35.1 37Y244S (TAC→TCC) lptDLPS assembly protein LptD
*NZ_CP0092734,254,0200AG57.9% 8.8 / 16.1 19I47T (ATC→ACC) qorAquinone oxidoreductase
*NZ_CP009273353,6010CA55.6% ‑3.4 / 16.7 18L267F (TTG→TTTcynRtranscriptional regulator CynR
*NZ_CP00927370,0680AC54.5% 14.3 / 25.8 22V314G (GTA→GGA) thiPthiamine/thiamine pyrophosphate ABC transporter permease ThiP
*NZ_CP009273653,1320AC54.5% 6.4 / 10.3 11V88G (GTG→GGG) crcBfluoride efflux transporter CrcB
*NZ_CP009273765,6700GT53.8% 7.0 / 14.6 13S746I (AGT→ATT) mngBmannosylglycerate hydrolase
*NZ_CP009273832,0480TG53.8% 8.3 / 13.7 13V81G (GTC→GGC) hcxBhydroxycarboxylate dehydrogenase HcXB
*NZ_CP009273172,7550AG53.6% 16.3 / 31.2 29T388A (ACG→GCG) clcAH(+)/Cl(‑) exchange transporter ClcA
*NZ_CP009273630,5480AT53.3% 3.1 / 17.5 15D95E (GAT→GAAybdMParB/RepB/Spo0J family partition protein
*NZ_CP009273711,0810AC53.3% 9.3 / 13.9 15T72P (ACC→CCC) BW25113_RS03560lipoprotein
*NZ_CP009273807,1770TG53.3% 8.6 / 14.3 16V67G (GTG→GGG) bioCmalonyl‑ACP O‑methyltransferase BioC
*NZ_CP0092731,548,2570TG52.9% 15.3 / 17.7 17Y557S (TAC→TCC) maeAmalate dehydrogenase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 3920778 =24 (0.880)3 (0.130) 3/144 2.0 12.3% coding (264/993 nt) asnA aspartate‑‑ammonia ligase
?NZ_CP009273 3920817 = 22 (0.940)coding (303/993 nt) asnA aspartate‑‑ammonia ligase
* ? NZ_CP009273 331139 =85 (3.110)4 (0.150) 3/160 2.2 4.6% coding (149/606 nt) yahD ankyrin repeat domain‑containing protein
?NZ_CP009273 331150 = 85 (3.260)coding (160/606 nt) yahD ankyrin repeat domain‑containing protein