breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 168 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273832,0420CG67.9% ‑2.8 / 24.7 28A79G (GCG→GGG) hcxBhydroxycarboxylate dehydrogenase HcXB
*NZ_CP009273832,0480TG64.3% 10.6 / 20.6 28V81G (GTC→GGC) hcxBhydroxycarboxylate dehydrogenase HcXB
*NZ_CP0092732,436,0610TG61.1% 6.8 / 11.5 18G273G (GGT→GGGmnmCbifunctional tRNA (5‑methylaminomethyl‑2‑thiouridine)(34)‑methyltransferase MnmD/FAD‑dependent 5‑carboxymethylaminomethyl‑2‑thiouridine(34) oxidoreductase MnmC
*NZ_CP009273822,9550TG60.0% 21.2 / 28.8 30T495P (ACC→CCC) ybhFATP‑binding cassette domain‑containing protein
*NZ_CP009273595,0350TG55.6% ‑4.2 / 25.0 27V289G (GTG→GGG) cusACu(+)/Ag(+) efflux RND transporter permease subunit CusA
*NZ_CP0092731,906,0070TG55.6% 17.2 / 27.6 27H276P (CAC→CCC) htpXprotease HtpX
*NZ_CP0092731,906,0110TG55.6% 11.1 / 26.8 27T275P (ACC→CCC) htpXprotease HtpX
*NZ_CP0092732,089,5670TG55.6% 22.5 / 29.4 27G81G (GGT→GGGhisFimidazole glycerol phosphate synthase subunit HisF
*NZ_CP0092734,594,1610CT54.5% 15.4 / 86.5 66S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP0092732,367,4000AC54.2% 27.9 / 50.7 48G361G (GGT→GGGmenEo‑succinylbenzoate‑‑CoA ligase
*NZ_CP009273766,3530TG53.6% 15.1 / 31.3 28intergenic (+286/‑561)mngB/cydAmannosylglycerate hydrolase/cytochrome ubiquinol oxidase subunit I
*NZ_CP009273353,6010CA53.3% 4.2 / 17.7 15L267F (TTG→TTTcynRtranscriptional regulator CynR
*NZ_CP0092732,601,6030TG53.3% 16.5 / 22.8 30V443G (GTG→GGG) hyfFhydrogenase 4 subunit F
*NZ_CP0092731,548,2570TG53.1% 20.5 / 28.2 32Y557S (TAC→TCC) maeAmalate dehydrogenase
*NZ_CP0092734,594,1670CT53.0% 28.8 / 91.2 66S64F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP009273822,9630TG52.9% 10.4 / 37.4 34D492A (GAC→GCC) ybhFATP‑binding cassette domain‑containing protein
*NZ_CP0092732,380,3410GA52.8% 7.1 / 47.8 36intergenic (+31/‑72)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
*NZ_CP0092732,380,3620CT52.6% 9.2 / 53.9 38intergenic (+52/‑51)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
*NZ_CP0092733,806,4320AC52.6% 47.8 / 67.6 60T114T (ACA→ACCcoaBCbifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate‑‑cysteine ligase CoaBC
*NZ_CP0092733,214,1260TC52.2% 16.5 / 32.0 23I425T (ATC→ACC) ygjGputrescine aminotransferase

Marginal new junction evidence (lowest skew 10 of 19 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 3000957 =51 (0.930)5 (0.110) 5/134 2.9 11.7% coding (89/1197 nt) dpaL diaminopropionate ammonia‑lyase
?NZ_CP009273 3000973 = 34 (0.760)coding (105/1197 nt) dpaL diaminopropionate ammonia‑lyase
* ? NZ_CP009273 3613238 =NA (NA)5 (0.100) 5/148 3.2 100% coding (687/4236 nt) rhsB rhs element protein RhsB
?NZ_CP009273 3613252 = 0 (0.000)coding (701/4236 nt) rhsB rhs element protein RhsB
* ? NZ_CP009273 1203246 =60 (1.090)4 (0.090) 4/134 3.3 9.7% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 26 (0.580)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 250217336 (0.650)4 (0.080) 4/146 3.5 10.4% coding (733/966 nt) glk glucokinase
?NZ_CP009273 = 2502211 37 (0.760)coding (695/966 nt) glk glucokinase
* ? NZ_CP009273 1040657 =42 (0.760)4 (0.080) 4/148 3.5 9.6% coding (603/1140 nt) gfcE polysaccharide export protein
?NZ_CP009273 1040766 = 38 (0.770)coding (494/1140 nt) gfcE polysaccharide export protein
* ? NZ_CP009273 = 123805036 (0.650)3 (0.070) 3/130 3.6 8.5% coding (487/915 nt) ldcA muramoyltetrapeptide carboxypeptidase
?NZ_CP009273 = 1238032 36 (0.830)coding (505/915 nt) ldcA muramoyltetrapeptide carboxypeptidase
* ? NZ_CP009273 1238033 =36 (0.650)3 (0.070) 3/130 3.6 8.5% coding (504/915 nt) ldcA muramoyltetrapeptide carboxypeptidase
?NZ_CP009273 1238051 = 36 (0.830)coding (486/915 nt) ldcA muramoyltetrapeptide carboxypeptidase
* ? NZ_CP009273 = 3935344NA (NA)4 (0.080) 4/156 3.7 NA noncoding (177/1542 nt) BW25113_RS19520 16S ribosomal RNA
?NZ_CP009273 = 4029052 NA (NA)noncoding (162/1542 nt) BW25113_RS19980 16S ribosomal RNA
* ? NZ_CP009273 = 3935334NA (NA)4 (0.080) 4/156 3.7 NA noncoding (167/1542 nt) BW25113_RS19520 16S ribosomal RNA
?NZ_CP009273 = 4029062 NA (NA)noncoding (172/1542 nt) BW25113_RS19980 16S ribosomal RNA
* ? NZ_CP009273 3421945 =NA (NA)4 (0.080) 4/156 3.7 NA noncoding (177/1542 nt) BW25113_RS17000 16S ribosomal RNA
?NZ_CP009273 3421960 = NA (NA)noncoding (162/1542 nt) BW25113_RS17000 16S ribosomal RNA