breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,791 G→A intergenic (+160/‑32) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,903 T→G D27E (GAT→GAG folA → type 3 dihydrofolate reductase
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 3,390,883 Δ1 bp coding (272/1470 nt) rng ← ribonuclease G
RA 3,632,398 A→C Q466P (CAG→CCG)  pitA → inorganic phosphate transporter PitA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 255700 260589 4890 29 [1] [1] 2 [phoE]–[yafX] [phoE],proB,proA,BW25113_RS01235,ykfI,yafW,ykfH,ykfG,[yafX]
* * ÷ NZ_CP009273 260658 263767 3110 2 [1] [1] 2 [yafX]–yafZ [yafX],ykfF,BW25113_RS01265,BW25113_RS25425,BW25113_RS25430,BW25113_RS25435,yafZ
* * ÷ NZ_CP009273 263858 264745 888 2 [1] [0] 2 [ykfA] [ykfA]
* * ÷ NZ_CP009273 264798 266919–266252 1455–2122 2 [0] [1] 2 perR–[BW25113_RS01300] perR,BW25113_RS01295,[BW25113_RS01300]
* * ÷ NZ_CP009273 356981 357567 587 4 [0] [0] 3 lacA galactoside O‑acetyltransferase
* * ÷ NZ_CP009273 488912 488920 9 2 [0] [1] 2 dnaX DNA polymerase III subunit gamma/tau
* * ÷ NZ_CP009273 989348 989360 13 2 [1] [1] 2 ssuB aliphatic sulfonates ABC transporter ATP‑binding protein
* * ÷ NZ_CP009273 1137520 1137561 42 2 [1] [1] 2 rne ribonuclease E
* * ÷ NZ_CP009273 1320872 1320940 69 2 [1] [0] 2 [yciQ] [yciQ]
* * ÷ NZ_CP009273 1357511 1357553 43 3 [1] [1] 2 puuC aldehyde dehydrogenase PuuC
* * ÷ NZ_CP009273 2368161 2368209 49 3 [1] [1] 2 menE o‑succinylbenzoate‑‑CoA ligase
* * ÷ NZ_CP009273 2505242 2505277 36 2 [1] [1] 7 mntH Nramp family divalent metal transporter
* * ÷ NZ_CP009273 2559761 2559791 31 3 [0] [1] 2 eutA ethanolamine ammonia‑lyase reactivating factor EutA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 = 2556991 (0.050)28 (1.280) 19/170 0.2 96.5% coding (113/1056 nt) phoE phosphoporin PhoE
?NZ_CP009273 269666 = NA (NA)intergenic (+2/+146) ykfC/BW25113_RS01325 reverse transcriptase domain‑containing protein/IS5‑like element IS5 family transposase
* ? NZ_CP009273 295395 =12 (0.550)4 (0.210) 4/150 1.3 28.0% coding (1251/2199 nt) paoC aldehyde oxidoreductase molybdenum‑binding subunit PaoC
?NZ_CP009273 295468 = 10 (0.520)coding (1178/2199 nt) paoC aldehyde oxidoreductase molybdenum‑binding subunit PaoC
* ? NZ_CP009273 360104 =NA (NA)42 (1.920) 23/170 0.1 NA intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 NA (NA)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 360168 =NA (NA)8 (0.410) 5/152 1.2 NA intergenic (‑99/+187) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 360191 = NA (NA)intergenic (‑122/+164) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 376748 =NA (NA)4 (0.200) 4/152 1.4 NA intergenic (‑5/‑59) BW25113_RS01855/BW25113_RS01870 transferase/IS3‑like element IS2 family transposase
?NZ_CP009273 376768 = NA (NA)intergenic (‑25/‑39) BW25113_RS01855/BW25113_RS01870 transferase/IS3‑like element IS2 family transposase
* ? NZ_CP009273 619757 =12 (0.550)3 (0.150) 3/152 1.6 21.6% coding (210/957 nt) fepB Fe2+‑enterobactin ABC transporter substrate‑binding protein
?NZ_CP009273 619788 = 11 (0.560)coding (179/957 nt) fepB Fe2+‑enterobactin ABC transporter substrate‑binding protein
* ? NZ_CP009273 3022788 =10 (0.460)3 (0.150) 3/156 1.6 23.8% coding (1503/1920 nt) ygfT formate‑dependent uric acid utilization protein YgfT
?NZ_CP009273 3022819 = 10 (0.500)coding (1472/1920 nt) ygfT formate‑dependent uric acid utilization protein YgfT
* ? NZ_CP009273 4532499 =1 (0.050)22 (1.130) 17/152 0.2 88.2% intergenic (+49/‑433) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 4532813 = 5 (0.260)intergenic (+363/‑119) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NZ_CP009273 = 45325071 (0.050)18 (0.920) 12/152 0.4 85.9% intergenic (+57/‑425) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 = 4532803 5 (0.260)intergenic (+353/‑129) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA