breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 44 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NZ_CP009273 | 4,225,954 | 0 | A | G | 62.5% | ‑0.1 / 15.4 | 16 | S41G (AGT→GGT) | yjbE | exopolysaccharide production protein YjbE |
* | NZ_CP009273 | 4,245,973 | 0 | T | G | 61.1% | 9.0 / 14.5 | 18 | N141T (AAC→ACC) | plsB | glycerol‑3‑phosphate 1‑O‑acyltransferase PlsB |
* | NZ_CP009273 | 5,159 | 0 | G | A | 60.0% | ‑0.9 / 20.3 | 20 | intergenic (+139/‑75) | thrC/yaaX | threonine synthase/DUF2502 domain‑containing protein |
* | NZ_CP009273 | 536,014 | 0 | T | G | 58.3% | 3.9 / 10.6 | 12 | intergenic (+50/‑7) | allB/ybbY | allantoinase AllB/uracil/xanthine transporter |
* | NZ_CP009273 | 536,016 | 0 | T | G | 58.3% | 7.2 / 11.0 | 12 | intergenic (+52/‑5) | allB/ybbY | allantoinase AllB/uracil/xanthine transporter |
* | NZ_CP009273 | 1,571,380 | 0 | A | C | 57.9% | 10.8 / 21.5 | 19 | V166G (GTA→GGA) | yddB | TonB‑dependent receptor plug domain‑containing protein |
* | NZ_CP009273 | 4,142,237 | 0 | T | G | 57.9% | 6.4 / 15.0 | 19 | T264P (ACC→CCC) | ppc | phosphoenolpyruvate carboxylase |
* | NZ_CP009273 | 2,002,571 | 0 | A | G | 57.1% | 9.2 / 15.0 | 14 | I272V (ATC→GTC) | yedE | selenium metabolism membrane protein YedE/FdhT |
* | NZ_CP009273 | 3,775,223 | 0 | T | G | 57.1% | 4.9 / 11.1 | 15 | T234P (ACC→CCC) | cysE | serine O‑acetyltransferase |
* | NZ_CP009273 | 3,941,183 | 0 | T | G | 56.2% | 5.4 / 16.8 | 16 | T49P (ACC→CCC) | hdfR | HTH‑type transcriptional regulator HdfR |
* | NZ_CP009273 | 700,041 | 0 | T | G | 55.6% | 14.7 / 19.4 | 19 | G214G (GGT→GGG) | nagE | PTS N‑acetyl glucosamine transporter subunit IIABC |
* | NZ_CP009273 | 1,240,703 | 0 | G | T | 55.6% | 5.6 / 12.7 | 9 | E30* (GAA→TAA) | BW25113_RS06225 | hypothetical protein |
* | NZ_CP009273 | 2,380,341 | 0 | G | A | 55.0% | ‑3.3 / 22.5 | 20 | intergenic (+31/‑72) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
* | NZ_CP009273 | 4,612,067 | 0 | A | G | 54.2% | 9.8 / 27.7 | 24 | E161G (GAG→GGG) | yjjJ | type II toxin‑antitoxin system HipA family toxin YjjJ |
* | NZ_CP009273 | 7,132 | 0 | A | C | 53.8% | 4.6 / 12.5 | 13 | G276G (GGT→GGG) | yaaJ | sodium:alanine symporter family protein |
* | NZ_CP009273 | 56,379 | 0 | T | G | 53.8% | 10.2 / 23.3 | 26 | Y244S (TAC→TCC) | lptD | LPS assembly protein LptD |
* | NZ_CP009273 | 536,033 | 0 | G | T | 53.8% | 7.0 / 20.7 | 13 | A5S (GCA→TCA) | ybbY | uracil/xanthine transporter |
* | NZ_CP009273 | 886,487 | 0 | C | T | 53.8% | 1.0 / 12.9 | 13 | S40F (TCT→TTT) | ybjC | DUF1418 family protein |
* | NZ_CP009273 | 2,794,508 | 0 | T | G | 53.3% | 9.8 / 15.9 | 15 | V62G (GTA→GGA) | nrdI | class Ib ribonucleoside‑diphosphate reductase assembly flavoprotein NrdI |
* | NZ_CP009273 | 4,594,161 | 0 | C | T | 53.3% | 12.8 / 40.1 | 30 | S62F (TCT→TTT) | bglJ | DNA‑binding transcriptional activator BglJ |
Marginal new junction evidence (lowest skew 10 of 13 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NZ_CP009273 | 2685639 = | 22 (1.010) | 5 (0.260) | 5/152 | 1.2 | 19.7% | coding (3264/3888 nt) | purL | phosphoribosylformylglycinamidine synthase |
? | NZ_CP009273 | 2685762 = | 21 (1.080) | coding (3141/3888 nt) | purL | phosphoribosylformylglycinamidine synthase | |||||
* | ? | NZ_CP009273 | 3574719 = | 29 (1.330) | 4 (0.200) | 4/156 | 1.4 | 12.6% | coding (222/489 nt) | yhhY | N‑acetyltransferase |
? | NZ_CP009273 | 3574782 = | 29 (1.450) | coding (285/489 nt) | yhhY | N‑acetyltransferase | |||||
* | ? | NZ_CP009273 | 4398991 = | 42 (1.920) | 3 (0.170) | 3/140 | 1.5 | 9.4% | intergenic (+79/‑101) | rnr/rlmB | ribonuclease R/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB |
? | NZ_CP009273 | 4399052 = | 23 (1.280) | intergenic (+140/‑40) | rnr/rlmB | ribonuclease R/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB | |||||
* | ? | NZ_CP009273 | 4306428 = | 24 (1.100) | 3 (0.160) | 3/150 | 1.6 | 13.0% | coding (608/1137 nt) | phnM | alpha‑D‑ribose 1‑methylphosphonate 5‑triphosphate diphosphatase |
? | NZ_CP009273 | 4306531 = | 19 (0.990) | coding (505/1137 nt) | phnM | alpha‑D‑ribose 1‑methylphosphonate 5‑triphosphate diphosphatase | |||||
* | ? | NZ_CP009273 | 3991398 = | 23 (1.050) | 3 (0.150) | 3/156 | 1.6 | 12.5% | coding (56/2163 nt) | uvrD | DNA helicase II |
? | NZ_CP009273 | 3991437 = | 21 (1.050) | coding (95/2163 nt) | uvrD | DNA helicase II | |||||
* | ? | NZ_CP009273 | = 3693271 | 23 (1.050) | 3 (0.150) | 3/152 | 1.6 | 12.3% | intergenic (+18/+69) | bcsG/ldrD | cellulose biosynthesis protein BcsG/type I toxin‑antitoxin system toxic polypeptide LdrD |
? | NZ_CP009273 | = 3693281 | 22 (1.130) | intergenic (+28/+59) | bcsG/ldrD | cellulose biosynthesis protein BcsG/type I toxin‑antitoxin system toxic polypeptide LdrD | |||||
* | ? | NZ_CP009273 | = 3333655 | 9 (0.410) | 3 (0.150) | 3/152 | 1.6 | 19.3% | coding (22/978 nt) | yrbG | calcium/sodium antiporter |
? | NZ_CP009273 | = 3333682 | 17 (0.870) | coding (49/978 nt) | yrbG | calcium/sodium antiporter | |||||
* | ? | NZ_CP009273 | = 2845472 | 15 (0.690) | 3 (0.150) | 3/154 | 1.6 | 12.9% | coding (250/273 nt) | hypC | hydrogenase 3 maturation protein HypC |
? | NZ_CP009273 | = 2845516 | 27 (1.370) | coding (22/1122 nt) | hypD | hydrogenase formation protein HypD | |||||
* | ? | NZ_CP009273 | 2837257 = | 27 (1.240) | 3 (0.150) | 3/152 | 1.6 | 12.5% | coding (313/768 nt) | hycG | formate hydrogenlyase subunit HycG |
? | NZ_CP009273 | 2837317 = | 18 (0.920) | coding (253/768 nt) | hycG | formate hydrogenlyase subunit HycG | |||||
* | ? | NZ_CP009273 | = 2707188 | 20 (0.920) | 3 (0.160) | 3/148 | 1.6 | 13.2% | coding (934/1335 nt) | srmB | ATP‑dependent RNA helicase SrmB |
? | NZ_CP009273 | = 2707267 | 22 (1.160) | coding (1013/1335 nt) | srmB | ATP‑dependent RNA helicase SrmB |