breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 69 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273353,6010CA67.7% 7.3 / 22.8 31L267F (TTG→TTTcynRtranscriptional regulator CynR
*NZ_CP009273822,4140TC58.3% 5.7 / 10.7 12K99E (AAA→GAA) ybhSABC transporter permease
*NZ_CP0092731,673,5610AC56.2% 14.0 / 17.5 16Y293S (TAC→TCC) ydgHDUF1471 family protein YdgH
*NZ_CP0092734,594,1610CT55.9% 5.8 / 37.4 34S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP0092731,329,9480TG55.6% 19.1 / 21.5 18intergenic (+24/‑140)BW25113_RS25560/acnAhypothetical protein/aconitate hydratase AcnA
*NZ_CP009273832,0420CG55.0% 6.6 / 19.4 20A79G (GCG→GGG) hcxBhydroxycarboxylate dehydrogenase HcXB
*NZ_CP0092731,152,7310TA54.5% 6.6 / 13.8 11L167I (TTA→ATA) ycfHmetal‑dependent hydrolase
*NZ_CP0092733,781,3850TG54.5% 16.4 / 27.3 22G396G (GGT→GGGenvCmurein hydrolase activator EnvC
*NZ_CP0092731,975,9940CG53.8% 8.4 / 13.7 13intergenic (‑126/+41)otsB/araHtrehalose‑phosphatase/arabinose ABC transporter permease AraH
*NZ_CP0092732,380,3410GA53.8% ‑1.7 / 14.0 13intergenic (+31/‑72)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
*NZ_CP0092734,464,5320TG53.3% 56.3 / 60.9 62*198G (TGA→GGA) bdcRTetR/AcrR family transcriptional regulator
*NZ_CP009273546,7710AG52.9% 7.0 / 18.6 17T293A (ACC→GCC) ybcFcarbamate kinase
*NZ_CP0092733,214,0960TG52.6% 16.3 / 25.0 19V415G (GTG→GGG) ygjGputrescine aminotransferase
*NZ_CP0092733,991,5290AT52.6% 12.7 / 25.5 19T63S (ACC→TCC) uvrDDNA helicase II
*NZ_CP0092731,812,6420AC52.0% 19.6 / 30.6 25V39G (GTG→GGG) celF6‑phospho‑beta‑glucosidase
*NZ_CP009273108,7890TC51.3% 29.4 / 42.3 37S100G (AGC→GGC) zapDcell division protein ZapD
*NZ_CP0092735,1590GA50.0% 5.4 / 10.8 18intergenic (+139/‑75)thrC/yaaXthreonine synthase/DUF2502 domain‑containing protein
*NZ_CP009273700,9960CA50.0% 23.7 / 16.5 26P533T (CCG→ACG) nagEPTS N‑acetyl glucosamine transporter subunit IIABC
*NZ_CP0092734,594,1670CT50.0% 12.5 / 19.6 34S64F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP0092733,726,6400TA47.6% 18.9 / 10.3 21N360K (AAT→AAAxylGD‑xylose ABC transporter ATP‑binding protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 4532499 =10 (0.300)5 (0.170) 5/152 1.8 18.2% intergenic (+49/‑433) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 4532813 = 36 (1.200)intergenic (+363/‑119) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA