breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 25 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,868,7140CT69.7% 600.7 / inf 376P9L (CCA→CTA) ytfKconserved protein
*AP012306713,2440CT69.7% 586.9 / inf 379S21F (TCC→TTC) ompXouter membrane protein
*AP0123061,091,9920TG45.1% 306.8 / 80.0 226intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123061,944,2880AC35.1% 446.8 / 53.0 225G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP012306341,3770TG33.9% 195.9 / 21.4 126G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP012306644,4170GA31.4% 835.7 / inf 567intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123063,527,3680AC29.4% 521.1 / 55.1 232L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP0123061,226,5570AG27.7% 357.1 / 84.7 177G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123063,938,8870CT26.5% 507.1 / 129.0 238S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP012306800,6750AC26.2% 617.3 / 29.6 263T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP0123063,361,6170G.25.0% 851.1 / 293.5 285coding (1089/1869 nt)trkDpotassium transporter
*AP0123063,361,6180G.25.0% 857.3 / 293.5 285coding (1090/1869 nt)trkDpotassium transporter
*AP0123063,361,6190C.24.9% 861.1 / 295.5 285coding (1091/1869 nt)trkDpotassium transporter
*AP0123063,282,0760TG24.9% 536.1 / 14.4 238intergenic (‑79/+27)ilvB/ivbLacetolactate synthase I, large subunit/ilvB operon leader peptide
*AP0123062,212,7530AC24.1% 363.4 / 36.0 142Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
*AP0123063,590,3670AC23.9% 681.0 / 30.7 263T30P (ACC→CCC) fabRDNA‑binding transcriptional repressor
*AP0123062,758,9090TG23.7% 576.0 / 16.0 211G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP0123063,903,7910AG23.6% 586.5 / 54.7 242E189G (GAA→GGA) yjgJpredicted transcriptional regulator
*AP0123061,854,8510AC23.6% 696.0 / 62.5 275T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP0123063,645,6420AC22.5% 909.8 / 34.8 347T318P (ACC→CCC) aceBmalate synthase A

Marginal new junction evidence (lowest skew 10 of 166 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 3361620 =232 (0.710)80 (0.260) 70/284 4.5 26.5% coding (1092/1869 nt) trkD potassium transporter
?AP012306 = 3361616 222 (0.720)coding (1088/1869 nt) trkD potassium transporter
* ? AP012306 = 1075430451 (1.390)32 (0.100) 30/298 9.5 8.2% intergenic (‑192/+236) ldrB/ldrC toxic polypeptide, small/toxic polypeptide, small
?AP012306 1080383 = 269 (0.830)coding (1031/1254 nt) ychP predicted invasin
* ? AP012306 = 997497346 (1.060)16 (0.050) 15/268 12.2 5.3% coding (41/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?AP012306 997993 = 260 (0.890)coding (537/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? AP012306 = 2310287469 (1.440)17 (0.050) 17/298 12.3 4.6% coding (243/345 nt) ygaC predicted protein
?AP012306 2316570 = 236 (0.730)coding (26/1065 nt) proW glycine betaine transporter subunit
* ? AP012306 = 3460583295 (0.910)13 (0.040) 12/288 13.5 4.3% coding (210/1332 nt) pepQ proline dipeptidase
?AP012306 3460633 = 293 (0.930)coding (260/1332 nt) pepQ proline dipeptidase
* ? AP012306 = 1092512380 (1.170)15 (0.050) 10/258 13.5 4.6% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 287 (1.020)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 1092334347 (1.070)11 (0.040) 10/258 13.5 3.6% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 287 (1.020)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 3600658NA (NA)40 (0.130) 10/292 14.3 NA noncoding (2805/2904 nt) rrlB 23S ribosomal RNA (rrlB)
?AP012306 = 3642045 NA (NA)noncoding (2790/2904 nt) rrlE 23S ribosomal RNA (rrlE)
* ? AP012306 1080383 =269 (0.830)9 (0.030)
+21 bp
8/256 14.3 3.2% coding (1031/1254 nt) ychP predicted invasin
?AP012306 = 3139166 369 (1.140)intergenic (‑172/‑304) ldrD/yhjV toxic polypeptide, small/predicted transporter
* ? AP012306 = 215364NA (NA)10 (0.030) 9/288 14.6 NA noncoding (745/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 215372 NA (NA)noncoding (753/2905 nt) rrlH 23S ribosomal RNA (rrlH)