breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 26,455 T→C intergenic (+111/‑39) LLMG_RS00135 → / → LLMG_RS00140 tRNA‑Asn/PTS transporter subunit EIIC
RA 187,916 C→T G20R (GGA→AGA)  LLMG_RS01045 ← type II CAAX endopeptidase family protein
RA 218,269 +C coding (1042/1056 nt) LLMG_RS01195 → DUF2142 domain‑containing protein
RA 311,626 T→C C173R (TGC→CGC)  lmrC → multidrug efflux ABC transporter LmrCD subunit C
MC JC 384,965 533 bp→5 bp coding (205‑737/945 nt) LLMG_RS02010 ← L‑lactate dehydrogenase
RA 404,128 T→G intergenic (+83/‑4) groES → / → groL co‑chaperone GroES/chaperonin GroEL
RA 430,174 C→T intergenic (+196/‑97) LLMG_RS02235 → / → LLMG_RS02240 MurR/RpiR family transcriptional regulator/PTS sugar transporter subunit IIC
RA 430,179 T→G intergenic (+201/‑92) LLMG_RS02235 → / → LLMG_RS02240 MurR/RpiR family transcriptional regulator/PTS sugar transporter subunit IIC
RA 549,527 (T)6→7 intergenic (‑197/‑190) LLMG_RS02885 ← / → LLMG_RS02890 IS256‑like element IS905 family transposase/thymidine kinase
RA 589,181 +C intergenic (+35/+231) LLMG_RS03120 → / ← LLMG_RS03125 glycosyltransferase family 2 protein/hypothetical protein
RA 594,325 T→A I263K (ATA→AAA)  recJ → single‑stranded‑DNA‑specific exonuclease RecJ
RA 617,310 A→C S8S (TCA→TCC LLMG_RS03280 → magnesium transporter CorA family protein
RA 636,592 (A)7→6 coding (297/1164 nt) LLMG_RS03345 ← ABC transporter permease
RA 672,541 A→T L159L (CTA→CTT LLMG_RS03545 → helix‑turn‑helix transcriptional regulator
RA 672,705 T→G pseudogene (119/138 nt) LLMG_RS13115 → transposase
MC JC 752,091 Δ985 bp [pta] [pta]
RA 876,869 C→G H82D (CAT→GAT)  LLMG_RS04645 → ammonium transporter
RA 894,627 +G coding (1044/1062 nt) LLMG_RS04715 → DUF916 and DUF3324 domain‑containing protein
RA 912,618 T→A L46Q (CTA→CAA)  LLMG_RS04815 → hypothetical protein
RA 912,718 T→A N79K (AAT→AAA LLMG_RS04815 → hypothetical protein
RA 945,239 +C pseudogene (615/1147 nt) LLMG_RS13415 → IS3 family transposase
RA 977,528 Δ1 bp coding (1177/1197 nt) LLMG_RS05140 → extracellular solute‑binding protein
RA 1,007,288 C→T F230F (TTC→TTT LLMG_RS05310 → beta‑glucoside‑specific PTS transporter subunit IIABC
RA 1,061,785 A→T I36N (ATT→AAT)  LLMG_RS05590 ← TPM domain‑containing protein
MC JC 1,081,159 35 bp→8 bp coding (242‑276/978 nt) LLMG_RS05690 → L‑lactate dehydrogenase
RA 1,093,299 +C coding (3979/4050 nt) LLMG_RS13005 → Ig‑like domain‑containing protein
RA 1,093,562 +C pseudogene (205/276 nt) LLMG_RS13840 → hypothetical protein
RA 1,167,414 T→G intergenic (+64/‑55) LLMG_RS06065 → / → LLMG_RS06070 hypothetical protein/trypsin‑like peptidase domain‑containing protein
RA 1,210,283 (A)5→6 coding (515/525 nt) LLMG_RS13870 ← LacI family DNA‑binding transcriptional regulator
RA 1,223,555 Δ1 bp intergenic (+243/+302) LLMG_RS06320 → / ← LLMG_RS06325 hypothetical protein/MerR family transcriptional regulator
RA 1,223,557 2 bp→AG intergenic (+245/+299) LLMG_RS06320 → / ← LLMG_RS06325 hypothetical protein/MerR family transcriptional regulator
RA 1,277,684 T→A N231I (AAT→ATT)  LLMG_RS06625 ← Cna B‑type domain‑containing protein
RA 1,318,548 (A)7→6 coding (98/162 nt) LLMG_RS13880 → hypothetical protein
MC JC 1,397,012 512 bp→5 bp coding (215‑726/972 nt) LLMG_RS07210 ← L‑lactate dehydrogenase
RA 1,492,365 T→A L262L (CTA→CTT LLMG_RS07640 ← ATP‑binding protein
RA 1,500,910 C→A D145E (GAC→GAA LLMG_RS13905 → WYL domain‑containing protein
RA 1,532,424 G→C P159R (CCC→CGC)  LLMG_RS07840 ← hypothetical protein
RA 1,572,256 T→A intergenic (+54/+183) LLMG_RS08045 → / ← deoD hypothetical protein/purine‑nucleoside phosphorylase
RA 1,572,258 G→A intergenic (+56/+181) LLMG_RS08045 → / ← deoD hypothetical protein/purine‑nucleoside phosphorylase
RA 1,588,193 T→G Q297P (CAA→CCA)  LLMG_RS08135 ← nucleotide sugar dehydrogenase
RA 1,588,245 2 bp→AT coding (837‑838/1170 nt) LLMG_RS08135 ← nucleotide sugar dehydrogenase
RA 1,588,356 T→A N243Y (AAT→TAT)  LLMG_RS08135 ← nucleotide sugar dehydrogenase
RA 1,610,761 T→C I430V (ATT→GTT)  LLMG_RS08230 ← hypothetical protein
RA 1,614,130 +T coding (1149/1278 nt) LLMG_RS08245 ← citrate:cation symporter
RA 1,660,056 (T)7→8 intergenic (‑602/+100) LLMG_RS08470 ← / ← recN TMEM175 family protein/DNA repair protein RecN
RA 1,681,037 T→C E332G (GAA→GGA)  pepV ← dipeptidase PepV
RA 1,731,090 T→C G335G (GGA→GGG LLMG_RS08790 ← YibE/F family protein
RA 1,826,407 A→G Y12Y (TAT→TAC LLMG_RS09240 ← MBL fold metallo‑hydrolase
RA 1,826,465 (T)6→7 intergenic (‑23/+300) LLMG_RS09240 ← / ← LLMG_RS09245 MBL fold metallo‑hydrolase/cold‑shock protein
RA 1,838,903 T→C T74A (ACT→GCT)  LLMG_RS09315 ← MarR family transcriptional regulator
RA 1,853,472 C→A M85I (ATG→ATT LLMG_RS09365 ← glycogen/starch/alpha‑glucan phosphorylase
RA 1,854,998 T→A V85V (GTA→GTT glgA ← glycogen synthase GlgA
RA 1,887,895 G→T L533I (CTA→ATA)  pepF ← oligoendopeptidase F
RA 1,891,450 C→A W87L (TGG→TTG)  LLMG_RS09565 ← YdcF family protein
RA 1,916,379 Δ1 bp pseudogene (355/598 nt) LLMG_RS09685 ← HAD family hydrolase
RA 1,921,435 Δ1 bp coding (76/660 nt) LLMG_RS09710 ← VTT domain‑containing protein
RA 1,921,462 Δ1 bp coding (49/660 nt) LLMG_RS09710 ← VTT domain‑containing protein
RA 1,921,474 Δ1 bp coding (37/660 nt) LLMG_RS09710 ← VTT domain‑containing protein
RA 1,921,516 C→T intergenic (‑6/‑379) LLMG_RS09710 ← / → LLMG_RS09715 VTT domain‑containing protein/amino acid ABC transporter substrate‑binding protein/permease
RA 1,921,531 Δ1 bp intergenic (‑21/‑364) LLMG_RS09710 ← / → LLMG_RS09715 VTT domain‑containing protein/amino acid ABC transporter substrate‑binding protein/permease
RA 1,921,639 Δ1 bp intergenic (‑129/‑256) LLMG_RS09710 ← / → LLMG_RS09715 VTT domain‑containing protein/amino acid ABC transporter substrate‑binding protein/permease
RA 1,921,652 Δ1 bp intergenic (‑142/‑243) LLMG_RS09710 ← / → LLMG_RS09715 VTT domain‑containing protein/amino acid ABC transporter substrate‑binding protein/permease
RA 1,921,656 Δ1 bp intergenic (‑146/‑239) LLMG_RS09710 ← / → LLMG_RS09715 VTT domain‑containing protein/amino acid ABC transporter substrate‑binding protein/permease
RA 1,921,665 Δ1 bp intergenic (‑155/‑230) LLMG_RS09710 ← / → LLMG_RS09715 VTT domain‑containing protein/amino acid ABC transporter substrate‑binding protein/permease
RA 1,921,675 Δ1 bp intergenic (‑165/‑220) LLMG_RS09710 ← / → LLMG_RS09715 VTT domain‑containing protein/amino acid ABC transporter substrate‑binding protein/permease
RA 1,921,682 Δ1 bp intergenic (‑172/‑213) LLMG_RS09710 ← / → LLMG_RS09715 VTT domain‑containing protein/amino acid ABC transporter substrate‑binding protein/permease
RA 1,921,694 Δ1 bp intergenic (‑184/‑201) LLMG_RS09710 ← / → LLMG_RS09715 VTT domain‑containing protein/amino acid ABC transporter substrate‑binding protein/permease
RA 1,921,704 Δ1 bp intergenic (‑194/‑191) LLMG_RS09710 ← / → LLMG_RS09715 VTT domain‑containing protein/amino acid ABC transporter substrate‑binding protein/permease
RA 1,921,708 Δ1 bp intergenic (‑198/‑187) LLMG_RS09710 ← / → LLMG_RS09715 VTT domain‑containing protein/amino acid ABC transporter substrate‑binding protein/permease
RA 1,921,719 Δ1 bp intergenic (‑209/‑176) LLMG_RS09710 ← / → LLMG_RS09715 VTT domain‑containing protein/amino acid ABC transporter substrate‑binding protein/permease
RA 1,921,725 Δ1 bp intergenic (‑215/‑170) LLMG_RS09710 ← / → LLMG_RS09715 VTT domain‑containing protein/amino acid ABC transporter substrate‑binding protein/permease
RA 1,921,747 (T)5→4 intergenic (‑237/‑148) LLMG_RS09710 ← / → LLMG_RS09715 VTT domain‑containing protein/amino acid ABC transporter substrate‑binding protein/permease
RA 1,921,755 Δ1 bp intergenic (‑245/‑140) LLMG_RS09710 ← / → LLMG_RS09715 VTT domain‑containing protein/amino acid ABC transporter substrate‑binding protein/permease
RA 1,921,777 Δ1 bp intergenic (‑267/‑118) LLMG_RS09710 ← / → LLMG_RS09715 VTT domain‑containing protein/amino acid ABC transporter substrate‑binding protein/permease
RA 1,921,790 Δ1 bp intergenic (‑280/‑105) LLMG_RS09710 ← / → LLMG_RS09715 VTT domain‑containing protein/amino acid ABC transporter substrate‑binding protein/permease
RA 1,933,214 2 bp→CG coding (224‑225/714 nt) atpB ← F0F1 ATP synthase subunit A
RA 1,935,405 (T)7→6 pseudogene (1748/2210 nt) LLMG_RS09775 ← DNA internalization‑related competence protein ComEC/Rec2
RA 1,942,432 T→C I80V (ATC→GTC)  LLMG_RS09805 ← SLC13 family permease
RA 1,942,730 C→A intergenic (‑61/+328) LLMG_RS09805 ← / ← tenA SLC13 family permease/thiaminase II
RA 2,026,794 T→G E596A (GAA→GCA)  pnp ← polyribonucleotide nucleotidyltransferase
RA 2,112,674 +T pseudogene (303/1128 nt) LLMG_RS10765 ← winged helix DNA‑binding domain‑containing protein
RA 2,122,554 G→A S58L (TCG→TTG)  LLMG_RS10800 ← cyclopropane‑fatty‑acyl‑phospholipid synthase family protein
RA 2,122,558 C→T A57T (GCC→ACC)  LLMG_RS10800 ← cyclopropane‑fatty‑acyl‑phospholipid synthase family protein
RA 2,183,058 (A)5→4 coding (399/1161 nt) LLMG_RS11130 ← Rgg/GadR/MutR family transcriptional regulator
RA 2,230,259 Δ1 bp coding (1758/1779 nt) LLMG_RS11405 ← glycerophosphodiester phosphodiesterase
RA 2,383,055 Δ1 bp intergenic (+245/‑10) LLMG_RS12210 → / → adhE lactonase family protein/bifunctional acetaldehyde‑CoA/alcohol dehydrogenase
MC JC 2,383,067 Δ2,719 bp [adhE] [adhE]

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_009004 669805 671337–670201 397–1533 582 [1] [220] 228 LLMG_RS03520–[LLMG_RS13110] LLMG_RS03520,[LLMG_RS13110]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_009004 = 6698041 (0.000)578 (0.860) 232/294 0.6 99.8% intergenic (‑59/‑271) LLMG_RS03515/LLMG_RS03520 GNAT family N‑acetyltransferase/hypothetical protein
?NC_009004 = 675922 NA (NA)intergenic (+55/‑633) LLMG_RS03560/LLMG_RS13605 IS3 family transposase/hypothetical protein
* ? NC_009004 1298894 =NA (NA)598 (0.890) 230/294 0.6 54.9% coding (329/342 nt) LLMG_RS06700 AraC family ligand binding domain‑containing protein
?NC_009004 = 1615073 491 (0.730)coding (206/1278 nt) LLMG_RS08245 citrate:cation symporter
* ? NC_009004 1298894 =NA (NA)9124 (13.520) 292/294 0.0 99.7% coding (329/342 nt) LLMG_RS06700 AraC family ligand binding domain‑containing protein
?NC_009004 = 1621394 31 (0.050)intergenic (‑102/+106) LLMG_RS08265/LLMG_RS08270 2,3‑butanediol dehydrogenase/FtsW/RodA/SpoVE family cell cycle protein
* ? NC_009004 = 1586092NA (NA)647 (0.970) 190/292 1.8 NA coding (726/774 nt) LLMG_RS08120 hypothetical protein
?NC_009004 = 1632647 NA (NA)coding (128/217 nt) LLMG_RS08320 IS3‑like element IS981 family transposase
* ? NC_009004 1615066 =29 (0.040)9792 (14.510) 292/294 0.0 99.7% coding (213/1278 nt) LLMG_RS08245 citrate:cation symporter
?NC_009004 1646630 = NA (NA)intergenic (‑104/‑89) LLMG_RS08410/LLMG_RS08415 GTP pyrophosphokinase family protein/IS256‑like element IS905 family transposase
* ? NC_009004 1621387 =465 (0.690)683 (1.010) 249/294 0.2 59.5% intergenic (‑95/+113) LLMG_RS08265/LLMG_RS08270 2,3‑butanediol dehydrogenase/FtsW/RodA/SpoVE family cell cycle protein
?NC_009004 1859127 = NA (NA)coding (38/1458 nt) LLMG_RS09385 4‑alpha‑glucanotransferase