breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 320 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273353,5860AT53.3% 7.8 / 18.7 18N272K (AAT→AAAcynRtranscriptional regulator CynR
*NZ_CP0092734,612,0670AG41.0% 105.9 / 39.2 79E161G (GAG→GGG) yjjJtype II toxin‑antitoxin system HipA family toxin YjjJ
*NZ_CP0092734,612,0720AG40.5% 117.7 / 29.7 79N163D (AAT→GAT) yjjJtype II toxin‑antitoxin system HipA family toxin YjjJ
*NZ_CP0092734,036,3900AG39.2% 76.1 / 11.8 51N206D (AAT→GAT) srkAstress response kinase SrkA
*NZ_CP009273748,5810TA39.2% 111.8 / 30.7 74intergenic (‑330/+60)ybgD/gltAfimbrial protein/citrate synthase
*NZ_CP0092732,145,1670GT39.1% 32.1 / 10.3 46intergenic (+40/+25)yegJ/pphCDUF2314 domain‑containing protein/protein‑serine/threonine phosphatase PphC
*NZ_CP0092734,326,7180TA38.1% 115.3 / 38.7 84Y44F (TAT→TTT) adiCarginine/agmatine antiporter
*NZ_CP0092732,897,0510CA37.6% 189.7 / 48.2 101intergenic (+318/+1055)ygcE/queEFGGY‑family carbohydrate kinase/7‑carboxy‑7‑deazaguanine synthase QueE
*NZ_CP0092732,326,3020AG36.8% 132.9 / 32.8 87pseudogene (1490/4515 nt)BW25113_RS11635alpha‑2‑macroglobulin family protein
*NZ_CP0092733,738,8540CT36.7% 78.8 / 13.3 49R231C (CGC→TGC) yiaN2,3‑diketo‑L‑gulonate transporter large permease YiaN
*NZ_CP0092731,995,3920GA36.1% 131.9 / 32.8 83intergenic (‑122/+199)fliA/fliCRNA polymerase sigma factor FliA/H48 family flagellin FliC
*NZ_CP0092732,145,1110CA35.4% 107.9 / 16.5 48A149E (GCA→GAA) yegJDUF2314 domain‑containing protein
*NZ_CP009273748,5770CA35.1% 138.5 / 19.3 77intergenic (‑326/+64)ybgD/gltAfimbrial protein/citrate synthase
*NZ_CP0092731,995,3990AC35.0% 162.7 / 20.1 80intergenic (‑129/+192)fliA/fliCRNA polymerase sigma factor FliA/H48 family flagellin FliC
*NZ_CP0092731,009,4840AG34.4% 157.9 / 33.8 90D257G (GAT→GGT) pqiBintermembrane transport protein PqiB
*NZ_CP009273981,1730TG34.2% 165.5 / 17.8 79intergenic (‑8/+177)aspC/ompFaspartate transaminase/porin OmpF
*NZ_CP009273107,6820TG33.7% 176.9 / 17.6 90V51G (GTG→GGG) mutT8‑oxo‑dGTP diphosphatase MutT
*NZ_CP0092731,009,4790TG33.3% 187.0 / 16.5 90G255G (GGT→GGGpqiBintermembrane transport protein PqiB
*NZ_CP0092733,941,2100CT33.3% 135.5 / 27.2 72E40K (GAA→AAA) hdfRHTH‑type transcriptional regulator HdfR
*NZ_CP0092734,419,8010TG33.3% 231.2 / 18.3 102L41V (TTG→GTG) cycAD‑serine/D‑alanine/glycine transporter

Marginal new junction evidence (lowest skew 10 of 12 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 = 4532507121 (1.160)10 (0.100) 10/474 13.4 8.5% intergenic (+57/‑425) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 = 4532803 98 (0.980)intergenic (+353/‑129) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NZ_CP009273 4532499 =123 (1.180)9 (0.090) 9/474 14.0 7.6% intergenic (+49/‑433) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 4532813 = 99 (0.990)intergenic (+363/‑119) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NZ_CP009273 = 540775106 (1.020)4 (0.040) 4/468 17.4 3.8% coding (1045/1050 nt) allD ureidoglycolate dehydrogenase
?NZ_CP009273 = 540763 101 (1.020)intergenic (‑15/+7) allC/allD allantoate deiminase/ureidoglycolate dehydrogenase
* ? NZ_CP009273 = 3750037102 (0.980)4 (0.040) 4/484 17.7 4.0% coding (1151/1152 nt) yiaY L‑threonine dehydrogenase
?NZ_CP009273 = 3750053 91 (0.890)coding (1135/1152 nt) yiaY L‑threonine dehydrogenase
* ? NZ_CP009273 = 272274492 (0.890)3 (0.030) 3/468 18.3 3.0% noncoding (60/76 nt) BW25113_RS13530 tRNA‑Glu
?NZ_CP009273 4199773 = 106 (1.070)noncoding (72/76 nt) BW25113_RS20795 tRNA‑Glu
* ? NZ_CP009273 = 272274492 (0.890)3 (0.030) 3/468 18.3 3.2% noncoding (60/76 nt) BW25113_RS13530 tRNA‑Glu
?NZ_CP009273 3936866 = 92 (0.930)noncoding (72/76 nt) BW25113_RS19525 tRNA‑Glu
* ? NZ_CP009273 = 1171385111 (1.070)3 (0.030) 3/468 18.3 2.8% coding (503/1200 nt) lolC lipoprotein‑releasing ABC transporter permease subunit LolC
?NZ_CP009273 = 1171373 103 (1.040)coding (491/1200 nt) lolC lipoprotein‑releasing ABC transporter permease subunit LolC
* ? NZ_CP009273 = 3024757116 (1.120)3 (0.030) 3/478 18.5 2.6% coding (32/1449 nt) uacT urate/proton symporter UacT
?NZ_CP009273 = 3024779 114 (1.130)coding (54/1449 nt) uacT urate/proton symporter UacT
* ? NZ_CP009273 = 107913108 (1.040)3 (0.030) 3/482 18.6 2.8% coding (383/390 nt) mutT 8‑oxo‑dGTP diphosphatase MutT
?NZ_CP009273 = 107931 104 (1.020)intergenic (+11/+205) mutT/yacG 8‑oxo‑dGTP diphosphatase MutT/DNA gyrase inhibitor YacG
* ? NZ_CP009273 = 2064735113 (1.090)3 (0.030) 3/488 18.7 2.7% intergenic (+43/‑285) BW25113_RS10440/agn43 GTPase family protein/autotransporter adhesin Ag43
?NZ_CP009273 = 2064750 107 (1.040)intergenic (+58/‑270) BW25113_RS10440/agn43 GTPase family protein/autotransporter adhesin Ag43