breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 267 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,515,9090AC62.5% 2.2 / 57.2 56V260G (GTG→GGG) ypfIpredicted hydrolase
*REL6064330TG49.5% 96.4 / 64.4 105V33G (GTG→GGG) thrAbifunctional aspartokinase I/homeserine dehydrogenase I
*REL6064,111,4290TG49.1% 57.9 / 19.8 59G496G (GGT→GGGmetLbifunctional aspartate kinase II/homoserine dehydrogenase II
*REL606895,8560GC48.6% 23.8 / 31.3 35A26P (GCC→CCC) ECB_00836putative Regulatory protein
*REL6064,180,2490TG45.8% 172.9 / 54.0 131G24G (GGT→GGGyjaHhypothetical protein
*REL6062,376,3480TG44.4% 242.5 / 70.6 187T63P (ACC→CCC) folCbifunctional folylpolyglutamate synthase/ dihydrofolate synthase
*REL6061,854,4010AC43.2% 126.4 / 53.8 111K88N (AAA→AACyeaOhypothetical protein
*REL6062,144,2950AC43.2% 309.7 / 122.3 220G357G (GGT→GGGmrpantiporter inner membrane protein
*REL6061,747,9030GT42.3% 722.9 / inf 655A8S (GCA→TCA) ydiLhypothetical protein
*REL606239,1630TG42.0% 222.7 / 139.3 181V58G (GTG→GGG) dnaQDNA polymerase III subunit epsilon
*REL6063,217,4340GC41.5% 12.9 / 27.2 41A76P (GCC→CCC) agaStagatose‑6‑phosphate ketose/aldose isomerase
*REL6062,304,4070CA41.4% 150.1 / 135.4 116Q266K (CAA→AAA) yfaDhypothetical protein
*REL6063,774,9140CG41.0% 207.3 / 144.5 156A20P (GCC→CCC) yicJpredicted transporter
*REL6061,149,6930TG40.4% 242.2 / 37.9 141G132G (GGT→GGGflgGflagellar component of cell‑distal portion of basal‑body rod
*REL6064,169,2250TG40.2% 647.9 / 49.8 343intergenic (+41/‑172)rpoC/htrCDNA‑directed RNA polymerase subunit beta'/heat shock protein
*REL6064,392,5560CT40.1% 164.9 / 128.1 187L43L (CTA→TTA) aidBisovaleryl CoA dehydrogenase
*REL6064,018,1740CT40.0% 31.8 / 12.6 26noncoding (2283/2904 nt)rrlA23S ribosomal RNA
*REL6061,798,7500AC38.4% 489.2 / 151.1 277G45G (GGT→GGGcelAN,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS
*REL6061,632,9640TG37.9% 581.0 / 110.8 301intergenic (‑96/‑39)ynfA/ynfBhypothetical protein/hypothetical protein
*REL606476,7460TG37.8% 258.2 / 28.9 135Q6H (CAA→CACfsrpredicted fosmidomycin efflux system

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 379585414 (1.460)185 (0.650) 8/150 9.8 30.6% intergenic (+110/+135) yajF/araJ fructokinase/predicted transporter
?REL606 = 2583282 424 (1.500)coding (741/741 nt) yfhQ predicted methyltransferase
* ? REL606 436399 =445 (1.570)4 (0.020) 4/130 11.0 1.1% intergenic (+36/‑70) ybaV/ybaW hypothetical protein/hypothetical protein
?REL606 436424 = 337 (1.370)intergenic (+61/‑45) ybaV/ybaW hypothetical protein/hypothetical protein
* ? REL606 = 379578453 (1.600)7 (0.030)
+C
5/148 11.2 1.9% intergenic (+103/+142) yajF/araJ fructokinase/predicted transporter
?REL606 2783323 = 268 (0.950)intergenic (+52/+96) ygcP/ygcQ predicted anti‑terminator regulatory protein/predicted flavoprotein
* ? REL606 3594449 =367 (1.290)4 (0.010) 4/142 11.5 0.95% intergenic (‑340/+28) yhiW/gadX DNA‑binding transcriptional activator/DNA‑binding transcriptional dual regulator
?REL606 4022367 = 487 (1.820)intergenic (+26/‑129) dsbA/yihF periplasmic protein disulfide isomerase I/hypothetical protein
* ? REL606 3594449 =367 (1.290)4 (0.020)
+TGAATATTCA
3/130 11.6 1.2% intergenic (‑340/+28) yhiW/gadX DNA‑binding transcriptional activator/DNA‑binding transcriptional dual regulator
?REL606 3594449 = 367 (1.290)intergenic (‑340/+28) yhiW/gadX DNA‑binding transcriptional activator/DNA‑binding transcriptional dual regulator
* ? REL606 = 3409185252 (0.890)3 (0.010) 3/134 11.8 1.3% intergenic (‑119/‑9) yheR/yheS glutathione‑regulated potassium‑efflux system ancillary protein/fused predicted transporter subunits of ABC superfamily: ATP‑binding components
?REL606 = 3604915 247 (0.980)coding (638/1323 nt) yhjE predicted transporter
* ? REL606 = 769273216 (0.760)4 (0.020) 3/134 11.8 1.5% coding (194/1041 nt) galM galactose‑1‑epimerase (mutarotase)
?REL606 = 3143485 319 (1.260)intergenic (‑125/‑113) ygjD/rpsU O‑sialoglycoprotein endopeptidase/30S ribosomal protein S21
* ? REL606 2666985 =330 (1.160)3 (0.010) 3/136 11.9 1.1% coding (341/549 nt) rimM 16S rRNA‑processing protein
?REL606 = 3206733 219 (0.850)coding (1104/1335 nt) garP predicted (D)‑galactarate transporter
* ? REL606 4022363 =498 (1.760)4 (0.010) 3/144 12.2 0.99% intergenic (+22/‑133) dsbA/yihF periplasmic protein disulfide isomerase I/hypothetical protein
?REL606 4085862 = 322 (1.180)intergenic (+60/‑89) cpxP/fieF periplasmic protein combats stress/ferrous iron efflux protein F
* ? REL606 = 1297292330 (1.160)6 (0.020) 3/150 12.4 1.8% intergenic (‑378/‑99) adhE/ychE fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase/predicted inner membrane protein
?REL606 = 1297292 330 (1.160)intergenic (‑378/‑99) adhE/ychE fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase/predicted inner membrane protein