breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 267 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 2,515,909 | 0 | A | C | 62.5% | 2.2 / 57.2 | 56 | V260G (GTG→GGG) | ypfI | predicted hydrolase |
* | REL606 | 433 | 0 | T | G | 49.5% | 96.4 / 64.4 | 105 | V33G (GTG→GGG) | thrA | bifunctional aspartokinase I/homeserine dehydrogenase I |
* | REL606 | 4,111,429 | 0 | T | G | 49.1% | 57.9 / 19.8 | 59 | G496G (GGT→GGG) | metL | bifunctional aspartate kinase II/homoserine dehydrogenase II |
* | REL606 | 895,856 | 0 | G | C | 48.6% | 23.8 / 31.3 | 35 | A26P (GCC→CCC) | ECB_00836 | putative Regulatory protein |
* | REL606 | 4,180,249 | 0 | T | G | 45.8% | 172.9 / 54.0 | 131 | G24G (GGT→GGG) | yjaH | hypothetical protein |
* | REL606 | 2,376,348 | 0 | T | G | 44.4% | 242.5 / 70.6 | 187 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
* | REL606 | 1,854,401 | 0 | A | C | 43.2% | 126.4 / 53.8 | 111 | K88N (AAA→AAC) | yeaO | hypothetical protein |
* | REL606 | 2,144,295 | 0 | A | C | 43.2% | 309.7 / 122.3 | 220 | G357G (GGT→GGG) | mrp | antiporter inner membrane protein |
* | REL606 | 1,747,903 | 0 | G | T | 42.3% | 722.9 / inf | 655 | A8S (GCA→TCA) | ydiL | hypothetical protein |
* | REL606 | 239,163 | 0 | T | G | 42.0% | 222.7 / 139.3 | 181 | V58G (GTG→GGG) | dnaQ | DNA polymerase III subunit epsilon |
* | REL606 | 3,217,434 | 0 | G | C | 41.5% | 12.9 / 27.2 | 41 | A76P (GCC→CCC) | agaS | tagatose‑6‑phosphate ketose/aldose isomerase |
* | REL606 | 2,304,407 | 0 | C | A | 41.4% | 150.1 / 135.4 | 116 | Q266K (CAA→AAA) | yfaD | hypothetical protein |
* | REL606 | 3,774,914 | 0 | C | G | 41.0% | 207.3 / 144.5 | 156 | A20P (GCC→CCC) | yicJ | predicted transporter |
* | REL606 | 1,149,693 | 0 | T | G | 40.4% | 242.2 / 37.9 | 141 | G132G (GGT→GGG) | flgG | flagellar component of cell‑distal portion of basal‑body rod |
* | REL606 | 4,169,225 | 0 | T | G | 40.2% | 647.9 / 49.8 | 343 | intergenic (+41/‑172) | rpoC/htrC | DNA‑directed RNA polymerase subunit beta'/heat shock protein |
* | REL606 | 4,392,556 | 0 | C | T | 40.1% | 164.9 / 128.1 | 187 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
* | REL606 | 4,018,174 | 0 | C | T | 40.0% | 31.8 / 12.6 | 26 | noncoding (2283/2904 nt) | rrlA | 23S ribosomal RNA |
* | REL606 | 1,798,750 | 0 | A | C | 38.4% | 489.2 / 151.1 | 277 | G45G (GGT→GGG) | celA | N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS |
* | REL606 | 1,632,964 | 0 | T | G | 37.9% | 581.0 / 110.8 | 301 | intergenic (‑96/‑39) | ynfA/ynfB | hypothetical protein/hypothetical protein |
* | REL606 | 476,746 | 0 | T | G | 37.8% | 258.2 / 28.9 | 135 | Q6H (CAA→CAC) | fsr | predicted fosmidomycin efflux system |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | = 379585 | 414 (1.460) | 185 (0.650) | 8/150 | 9.8 | 30.6% | intergenic (+110/+135) | yajF/araJ | fructokinase/predicted transporter |
? | REL606 | = 2583282 | 424 (1.500) | coding (741/741 nt) | yfhQ | predicted methyltransferase | |||||
* | ? | REL606 | 436399 = | 445 (1.570) | 4 (0.020) | 4/130 | 11.0 | 1.1% | intergenic (+36/‑70) | ybaV/ybaW | hypothetical protein/hypothetical protein |
? | REL606 | 436424 = | 337 (1.370) | intergenic (+61/‑45) | ybaV/ybaW | hypothetical protein/hypothetical protein | |||||
* | ? | REL606 | = 379578 | 453 (1.600) | 7 (0.030) +C |
5/148 | 11.2 | 1.9% | intergenic (+103/+142) | yajF/araJ | fructokinase/predicted transporter |
? | REL606 | 2783323 = | 268 (0.950) | intergenic (+52/+96) | ygcP/ygcQ | predicted anti‑terminator regulatory protein/predicted flavoprotein | |||||
* | ? | REL606 | 3594449 = | 367 (1.290) | 4 (0.010) | 4/142 | 11.5 | 0.95% | intergenic (‑340/+28) | yhiW/gadX | DNA‑binding transcriptional activator/DNA‑binding transcriptional dual regulator |
? | REL606 | 4022367 = | 487 (1.820) | intergenic (+26/‑129) | dsbA/yihF | periplasmic protein disulfide isomerase I/hypothetical protein | |||||
* | ? | REL606 | 3594449 = | 367 (1.290) | 4 (0.020) +TGAATATTCA |
3/130 | 11.6 | 1.2% | intergenic (‑340/+28) | yhiW/gadX | DNA‑binding transcriptional activator/DNA‑binding transcriptional dual regulator |
? | REL606 | 3594449 = | 367 (1.290) | intergenic (‑340/+28) | yhiW/gadX | DNA‑binding transcriptional activator/DNA‑binding transcriptional dual regulator | |||||
* | ? | REL606 | = 3409185 | 252 (0.890) | 3 (0.010) | 3/134 | 11.8 | 1.3% | intergenic (‑119/‑9) | yheR/yheS | glutathione‑regulated potassium‑efflux system ancillary protein/fused predicted transporter subunits of ABC superfamily: ATP‑binding components |
? | REL606 | = 3604915 | 247 (0.980) | coding (638/1323 nt) | yhjE | predicted transporter | |||||
* | ? | REL606 | = 769273 | 216 (0.760) | 4 (0.020) | 3/134 | 11.8 | 1.5% | coding (194/1041 nt) | galM | galactose‑1‑epimerase (mutarotase) |
? | REL606 | = 3143485 | 319 (1.260) | intergenic (‑125/‑113) | ygjD/rpsU | O‑sialoglycoprotein endopeptidase/30S ribosomal protein S21 | |||||
* | ? | REL606 | 2666985 = | 330 (1.160) | 3 (0.010) | 3/136 | 11.9 | 1.1% | coding (341/549 nt) | rimM | 16S rRNA‑processing protein |
? | REL606 | = 3206733 | 219 (0.850) | coding (1104/1335 nt) | garP | predicted (D)‑galactarate transporter | |||||
* | ? | REL606 | 4022363 = | 498 (1.760) | 4 (0.010) | 3/144 | 12.2 | 0.99% | intergenic (+22/‑133) | dsbA/yihF | periplasmic protein disulfide isomerase I/hypothetical protein |
? | REL606 | 4085862 = | 322 (1.180) | intergenic (+60/‑89) | cpxP/fieF | periplasmic protein combats stress/ferrous iron efflux protein F | |||||
* | ? | REL606 | = 1297292 | 330 (1.160) | 6 (0.020) | 3/150 | 12.4 | 1.8% | intergenic (‑378/‑99) | adhE/ychE | fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase/predicted inner membrane protein |
? | REL606 | = 1297292 | 330 (1.160) | intergenic (‑378/‑99) | adhE/ychE | fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase/predicted inner membrane protein |