mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Marginal read alignment evidence (highest frequency 20 of 222 shown, sorted by frequency from high to low) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,118,209 | 0 | G | T | 50.9% | 2.4 / 84.4 | 57 | intergenic (‑444/‑311) | csgD/csgB | DNA‑binding transcriptional activator in two‑component regulatory system/curlin nucleator protein, minor subunit in curli complex |
| * | REL606 | 2,437,406 | 0 | G | T | 47.1% | 26.9 / 24.0 | 34 | D435E (GAC→GAA) | ypdD | fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component |
| * | REL606 | 1,747,903 | 0 | G | T | 46.5% | 322.4 / inf | 389 | A8S (GCA→TCA) | ydiL | hypothetical protein |
| * | REL606 | 433 | 0 | T | G | 44.9% | 111.1 / 41.5 | 89 | V33G (GTG→GGG) | thrA | bifunctional aspartokinase I/homeserine dehydrogenase I |
| * | REL606 | 2,376,348 | 0 | T | G | 44.4% | 184.3 / 37.9 | 144 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
| * | REL606 | 1,854,401 | 0 | A | C | 44.0% | 87.2 / 27.1 | 75 | K88N (AAA→AAC) | yeaO | hypothetical protein |
| * | REL606 | 4,180,249 | 0 | T | G | 43.6% | 147.7 / 72.3 | 126 | G24G (GGT→GGG) | yjaH | hypothetical protein |
| * | REL606 | 720,464 | 0 | C | G | 41.7% | 15.6 / 25.5 | 24 | A22G (GCC→GGC) | phrB | deoxyribodipyrimidine photolyase, FAD‑binding |
| * | REL606 | 1,875,483 | 0 | G | C | 41.3% | 540.0 / inf | 564 | intergenic (+156/‑28) | yeaB/sdaA | predicted NUDIX hydrolase/L‑serine deaminase I |
| * | REL606 | 3,099,091 | 0 | A | C | 40.2% | 200.8 / 18.6 | 112 | V352G (GTG→GGG) | parC | DNA topoisomerase IV subunit A |
| * | REL606 | 4,392,556 | 0 | C | T | 39.5% | 172.0 / 113.8 | 162 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
| * | REL606 | 2,515,909 | 0 | A | C | 38.4% | 116.1 / 25.3 | 73 | V260G (GTG→GGG) | ypfI | predicted hydrolase |
| * | REL606 | 4,111,429 | 0 | T | G | 38.0% | 115.0 / 18.8 | 71 | G496G (GGT→GGG) | metL | bifunctional aspartate kinase II/homoserine dehydrogenase II |
| * | REL606 | 3,783,565 | 0 | A | C | 37.8% | 181.3 / 53.3 | 119 | T744P (ACA→CCA) | ECB_03521 | conserved hypothetical protein |
| * | REL606 | 2,356,014 | 0 | T | G | 37.5% | 324.9 / 55.8 | 176 | T58P (ACC→CCC) | yfbU | hypothetical protein |
| * | REL606 | 1,061,648 | 0 | A | C | 37.2% | 282.3 / 39.2 | 145 | G40G (GGT→GGG) | yccY | phosphotyrosine‑protein phosphatase |
| * | REL606 | 4,595,070 | 0 | A | G | 36.4% | 283.4 / 108.1 | 173 | G11G (GGA→GGG) | yjjZ | hypothetical protein |
| * | REL606 | 239,163 | 0 | T | G | 35.8% | 360.3 / 111.4 | 204 | V58G (GTG→GGG) | dnaQ | DNA polymerase III subunit epsilon |
| * | REL606 | 4,169,225 | 0 | T | G | 35.0% | 391.8 / 15.3 | 180 | intergenic (+41/‑172) | rpoC/htrC | DNA‑directed RNA polymerase subunit beta'/heat shock protein |
| * | REL606 | 3,229,011 | 0 | A | C | 35.0% | 204.7 / 77.0 | 123 | T126T (ACA→ACC) | yraM | hypothetical protein |
| Marginal new junction evidence (lowest skew 10 of 15 shown) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | = 2056287 | 195 (0.780) | 77 (0.330) +CTTA |
18/142 | 10.7 | 15.6% | intergenic (‑191/+2) | cpsG/cpsB | phosphomannomutase/mannose‑1‑phosphate guanyltransferase |
| ? | REL606 | 4274785 = | 688 (2.760) | intergenic (+58/+473) | gltP/yjcO | glutamate/aspartate:proton symporter/hypothetical protein | |||||
| * | ? | REL606 | = 114315 | 395 (1.590) | 11 (0.060) +CAAATCCGAGCCGTA |
3/120 | 16.5 | 3.8% | intergenic (+78/+138) | mutT/yacG | nucleoside triphosphate pyrophosphohydrolase, marked preference for dGTP/zinc‑binding protein |
| ? | REL606 | = 2293898 | 300 (1.200) | intergenic (+48/‑194) | nrdA/nrdB | ribonucleotide‑diphosphate reductase alpha subunit/ribonucleotide‑diphosphate reductase beta subunit | |||||
| * | ? | REL606 | = 2293898 | 300 (1.200) | 11 (0.050) +TACGGCTCGGATTT |
3/122 | 16.6 | 4.1% | intergenic (+48/‑194) | nrdA/nrdB | ribonucleotide‑diphosphate reductase alpha subunit/ribonucleotide‑diphosphate reductase beta subunit |
| ? | REL606 | 2374569 = | 341 (1.370) | intergenic (‑214/+45) | cvpA/dedD | membrane protein required for colicin V production/hypothetical protein | |||||
| * | ? | REL606 | = 5598 | 434 (1.740) | 9 (0.040) +AAATCCGAGCCGTA |
3/122 | 16.6 | 2.9% | intergenic (+70/+83) | yaaX/yaaA | hypothetical protein/hypothetical protein |
| ? | REL606 | = 2293898 | 300 (1.200) | intergenic (+48/‑194) | nrdA/nrdB | ribonucleotide‑diphosphate reductase alpha subunit/ribonucleotide‑diphosphate reductase beta subunit | |||||
| * | ? | REL606 | = 2293898 | 300 (1.200) | 11 (0.050) +TACGGCTCGGATT |
3/124 | 16.7 | 3.5% | intergenic (+48/‑194) | nrdA/nrdB | ribonucleotide‑diphosphate reductase alpha subunit/ribonucleotide‑diphosphate reductase beta subunit |
| ? | REL606 | = 4005927 | 440 (1.770) | intergenic (+228/+55) | fre/fadA | NAD(P)H‑flavin reductase/acetyl‑CoA acetyltransferase | |||||
| * | ? | REL606 | = 2255061 | NA (NA) | 11 (0.050) +AATCCGAGCCGTA |
3/124 | 16.7 | 4.3% | intergenic (+679/+151) | eco/mqo | ecotin precursor/malate:quinone oxidoreductase |
| ? | REL606 | = 2293898 | 300 (1.200) | intergenic (+48/‑194) | nrdA/nrdB | ribonucleotide‑diphosphate reductase alpha subunit/ribonucleotide‑diphosphate reductase beta subunit | |||||
| * | ? | REL606 | = 2254722 | 66 (0.270) | 11 (0.050) +AATCCGAGCCGTA |
3/124 | 16.7 | 6.8% | intergenic (+340/+490) | eco/mqo | ecotin precursor/malate:quinone oxidoreductase |
| ? | REL606 | = 2293898 | 300 (1.200) | intergenic (+48/‑194) | nrdA/nrdB | ribonucleotide‑diphosphate reductase alpha subunit/ribonucleotide‑diphosphate reductase beta subunit | |||||
| * | ? | REL606 | = 2254496 | 108 (0.430) | 11 (0.050) +AATCCGAGCCGTA |
3/124 | 16.7 | 6.1% | intergenic (+114/+716) | eco/mqo | ecotin precursor/malate:quinone oxidoreductase |
| ? | REL606 | = 2293898 | 300 (1.200) | intergenic (+48/‑194) | nrdA/nrdB | ribonucleotide‑diphosphate reductase alpha subunit/ribonucleotide‑diphosphate reductase beta subunit | |||||
| * | ? | REL606 | 69410 = | NA (NA) | 9 (0.040) +AATCCGAGCCGTA |
3/124 | 16.7 | 3.5% | intergenic (‑56/+229) | araD/araA | L‑ribulose‑5‑phosphate 4‑epimerase/L‑arabinose isomerase |
| ? | REL606 | = 2293898 | 300 (1.200) | intergenic (+48/‑194) | nrdA/nrdB | ribonucleotide‑diphosphate reductase alpha subunit/ribonucleotide‑diphosphate reductase beta subunit | |||||
| * | ? | REL606 | = 2293898 | 300 (1.200) | 11 (0.050) +TACGGCTCGGA |
3/128 | 17.0 | 3.2% | intergenic (+48/‑194) | nrdA/nrdB | ribonucleotide‑diphosphate reductase alpha subunit/ribonucleotide‑diphosphate reductase beta subunit |
| ? | REL606 | = 2928231 | 475 (1.910) | intergenic (+185/+11) | ygfZ/yqfA | putative global regulator/predicted oxidoreductase, inner membrane subunit | |||||