breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 242,440 | A→T | intergenic (+58/‑373) | ECB_00212 → / → yhhI | hypothetical protein/predicted transposase |
RA | 474,668 | C→T | W213* (TGG→TAG) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
RA | 650,624 | T→G | K60Q (AAG→CAG) | mrdA ← | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
RA | 2,116,047 | (C)7→8 | coding (345/1218 nt) | ECB_02003 ← | hypothetical protein |
RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
JC | 3,014,068 | (AAC)3→2 | coding (649‑651/1137 nt) | ECB_02815 → | Capsule polysaccharide export inner‑membrane protein kpsE |
RA | 3,147,762 | G→A | intergenic (+57/+22) | rpoD → / ← ygjF | RNA polymerase sigma factor/G/U mismatch‑specific DNA glycosylase |
RA | 3,498,410 | A→C | S360A (TCG→GCG) | glgA ← | glycogen synthase |
RA | 3,755,256 | +A | coding (225/864 nt) | yicC → | hypothetical protein |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
RA | 4,162,570 | T→A | I572N (ATC→AAC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
RA | 4,438,531 | T→G | D110A (GAC→GCC) | fbp ← | fructose‑1,6‑bisphosphatase |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | 4260752 = | 401 (1.610) | 234 (0.940) | 97/150 | 0.6 | 36.9% | coding (1257/1293 nt) | yjcF | hypothetical protein |
? | REL606 | = 4506109 | NA (NA) | noncoding (1/768 nt) | IS1 | repeat region | |||||
* | ? | REL606 | = 4384397 | 325 (1.320) | 235 (0.960) | 99/148 | 0.5 | 42.0% | coding (53/426 nt) | yjeB | predicted DNA‑binding transcriptional regulator |
? | REL606 | 4505342 = | NA (NA) | noncoding (768/768 nt) | IS1 | repeat region |