breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 242,440 A→T intergenic (+58/‑373) ECB_00212 → / → yhhI hypothetical protein/predicted transposase
RA 474,668 C→T W213* (TGG→TAG)  ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
RA 650,624 T→G K60Q (AAG→CAG)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 2,116,047 (C)7→8 coding (345/1218 nt) ECB_02003 ← hypothetical protein
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
JC 3,014,068 (AAC)3→2 coding (649‑651/1137 nt) ECB_02815 → Capsule polysaccharide export inner‑membrane protein kpsE
RA 3,147,762 G→A intergenic (+57/+22) rpoD → / ← ygjF RNA polymerase sigma factor/G/U mismatch‑specific DNA glycosylase
RA 3,498,410 A→C S360A (TCG→GCG)  glgA ← glycogen synthase
RA 3,755,256 +A coding (225/864 nt) yicC → hypothetical protein
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 4,162,570 T→A I572N (ATC→AAC)  rpoB → DNA‑directed RNA polymerase subunit beta
RA 4,438,531 T→G D110A (GAC→GCC)  fbp ← fructose‑1,6‑bisphosphatase
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 4260752 =401 (1.610)234 (0.940) 97/150 0.6 36.9% coding (1257/1293 nt) yjcF hypothetical protein
?REL606 = 4506109 NA (NA)noncoding (1/768 nt) IS1 repeat region
* ? REL606 = 4384397325 (1.320)235 (0.960) 99/148 0.5 42.0% coding (53/426 nt) yjeB predicted DNA‑binding transcriptional regulator
?REL606 4505342 = NA (NA)noncoding (768/768 nt) IS1 repeat region