breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 244 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 720,466 | 0 | G | C | 63.2% | ‑3.9 / 14.3 | 19 | A23P (GCC→CCC) | phrB | deoxyribodipyrimidine photolyase, FAD‑binding |
* | REL606 | 2,515,909 | 0 | A | C | 55.6% | 56.3 / 94.0 | 63 | V260G (GTG→GGG) | ypfI | predicted hydrolase |
* | REL606 | 1,854,401 | 0 | A | C | 50.6% | 60.2 / 110.8 | 81 | K88N (AAA→AAC) | yeaO | hypothetical protein |
* | REL606 | 2,437,406 | 0 | G | T | 50.0% | 25.2 / 34.9 | 38 | D435E (GAC→GAA) | ypdD | fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component |
* | REL606 | 433 | 0 | T | G | 48.4% | 111.3 / 59.7 | 97 | V33G (GTG→GGG) | thrA | bifunctional aspartokinase I/homeserine dehydrogenase I |
* | REL606 | 4,180,249 | 0 | T | G | 46.9% | 139.0 / 39.7 | 96 | G24G (GGT→GGG) | yjaH | hypothetical protein |
* | REL606 | 720,464 | 0 | C | G | 44.0% | 8.5 / 27.7 | 25 | A22G (GCC→GGC) | phrB | deoxyribodipyrimidine photolyase, FAD‑binding |
* | REL606 | 4,111,429 | 0 | T | G | 41.3% | 89.8 / 13.7 | 63 | G496G (GGT→GGG) | metL | bifunctional aspartate kinase II/homoserine dehydrogenase II |
* | REL606 | 2,376,348 | 0 | T | G | 40.6% | 228.4 / 51.4 | 155 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
* | REL606 | 2,213,003 | 0 | A | C | 40.6% | 162.6 / 11.2 | 101 | V184G (GTG→GGG) | fruK | 1‑phosphofructokinase |
* | REL606 | 3,472,700 | 0 | C | G | 40.0% | 103.0 / 87.4 | 100 | A290G (GCG→GGG) | yhgA | predicted transposase |
* | REL606 | 2,822,106 | 0 | G | C | 38.8% | 240.8 / 91.1 | 129 | pseudogene (62/198 nt) | fucA | L‑fuculose‑1‑phosphate aldolase; b2800_1 |
* | REL606 | 2,073,523 | 0 | A | C | 37.5% | 226.5 / 52.3 | 120 | G432G (GGT→GGG) | asmA | predicted assembly protein |
* | REL606 | 2,676,636 | 0 | T | G | 37.2% | 350.4 / 68.2 | 200 | V149G (GTG→GGG) | smpB | SsrA‑binding protein |
* | REL606 | 3,378,885 | 0 | T | G | 36.6% | 424.2 / 57.6 | 202 | T129P (ACC→CCC) | rplB | 50S ribosomal protein L2 |
* | REL606 | 476,746 | 0 | T | G | 36.5% | 166.6 / 18.7 | 85 | Q6H (CAA→CAC) | fsr | predicted fosmidomycin efflux system |
* | REL606 | 4,392,556 | 0 | C | T | 36.2% | 217.7 / 89.5 | 152 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
* | REL606 | 1,096,032 | 0 | C | G | 36.1% | 211.3 / 97.6 | 119 | G148G (GGC→GGG) | putP | proline:sodium symporter |
* | REL606 | 4,169,225 | 0 | T | G | 35.9% | 490.3 / 19.9 | 223 | intergenic (+41/‑172) | rpoC/htrC | DNA‑directed RNA polymerase subunit beta'/heat shock protein |
* | REL606 | 1,118,209 | 0 | G | T | 35.4% | 56.6 / 60.4 | 65 | intergenic (‑444/‑311) | csgD/csgB | DNA‑binding transcriptional activator in two‑component regulatory system/curlin nucleator protein, minor subunit in curli complex |
Marginal new junction evidence (lowest skew 10 of 36 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | = 1506449 | 213 (0.920) | 6 (0.030) | 5/136 | 19.4 | 3.3% | coding (72/792 nt) | ydcC | hypothetical protein |
? | REL606 | = 3554988 | 162 (0.770) | intergenic (+168/‑78) | yrhC/yhhI | b4552; predicted protein fragment/predicted transposase | |||||
* | ? | REL606 | = 1506307 | 21 (0.090) | 6 (0.030) | 5/136 | 19.4 | 5.5% | intergenic (+169/‑71) | ECB_01417/ydcC | hypothetical protein/hypothetical protein |
? | REL606 | = 1506442 | 188 (0.890) | coding (65/792 nt) | ydcC | hypothetical protein | |||||
* | ? | REL606 | = 718914 | 82 (0.350) | 6 (0.030) | 5/136 | 19.4 | 4.4% | intergenic (+60/‑71) | yhhI/ybfD | predicted transposase; b3484_1/hypothetical protein |
? | REL606 | = 1506442 | 188 (0.890) | coding (65/792 nt) | ydcC | hypothetical protein | |||||
* | ? | REL606 | = 717648 | 203 (0.870) | 6 (0.030) | 5/136 | 19.4 | 3.1% | intergenic (+263/‑70) | ECB_00664/yhhI | hypothetical protein/predicted transposase; b3484_1 |
? | REL606 | = 1506442 | 188 (0.890) | coding (65/792 nt) | ydcC | hypothetical protein | |||||
* | ? | REL606 | 570007 = | 75 (0.320) | 6 (0.030) | 5/136 | 19.4 | 4.5% | intergenic (‑71/+608) | yhhI/ECB_00526 | predicted transposase/hypothetical protein |
? | REL606 | = 1506442 | 188 (0.890) | coding (65/792 nt) | ydcC | hypothetical protein | |||||
* | ? | REL606 | = 242742 | 103 (0.440) | 6 (0.030) | 5/136 | 19.4 | 4.1% | intergenic (+360/‑71) | ECB_00212/yhhI | hypothetical protein/predicted transposase |
? | REL606 | = 1506442 | 188 (0.890) | coding (65/792 nt) | ydcC | hypothetical protein | |||||
* | ? | REL606 | 2039202 = | 108 (0.460) | 4 (0.020) | 4/130 | 19.8 | 3.8% | coding (493/747 nt) | wbbA | putative glycosyltransferase WbbA |
? | REL606 | 2039549 = | 109 (0.540) | coding (146/747 nt) | wbbA | putative glycosyltransferase WbbA | |||||
* | ? | REL606 | = 2037392 | 133 (0.570) | 5 (0.020) | 4/136 | 20.2 | 3.7% | coding (739/1176 nt) | wzy | O antigen polymerase (Wzy) |
? | REL606 | = 2037686 | 138 (0.660) | coding (445/1176 nt) | wzy | O antigen polymerase (Wzy) | |||||
* | ? | REL606 | 2679207 = | 219 (0.940) | 4 (0.020) | 4/138 | 20.4 | 1.9% | coding (279/1557 nt) | ECB_02510 | Recombinase |
? | REL606 | 2679381 = | 213 (1.000) | coding (453/1557 nt) | ECB_02510 | Recombinase | |||||
* | ? | REL606 | = 2406673 | 266 (1.150) | 5 (0.020) | 4/138 | 20.4 | 1.9% | intergenic (+363/‑3) | fadL/yfdF | long‑chain fatty acid outer membrane transporter/putative DNA modification enzyme; b2345_1 |
? | REL606 | = 2407104 | 280 (1.310) | pseudogene (44/462 nt) | yfdF | putative DNA modification enzyme; b2345_1 |