breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 244 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL606720,4660GC63.2% ‑3.9 / 14.3 19A23P (GCC→CCC) phrBdeoxyribodipyrimidine photolyase, FAD‑binding
*REL6062,515,9090AC55.6% 56.3 / 94.0 63V260G (GTG→GGG) ypfIpredicted hydrolase
*REL6061,854,4010AC50.6% 60.2 / 110.8 81K88N (AAA→AACyeaOhypothetical protein
*REL6062,437,4060GT50.0% 25.2 / 34.9 38D435E (GAC→GAAypdDfused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component
*REL6064330TG48.4% 111.3 / 59.7 97V33G (GTG→GGG) thrAbifunctional aspartokinase I/homeserine dehydrogenase I
*REL6064,180,2490TG46.9% 139.0 / 39.7 96G24G (GGT→GGGyjaHhypothetical protein
*REL606720,4640CG44.0% 8.5 / 27.7 25A22G (GCC→GGC) phrBdeoxyribodipyrimidine photolyase, FAD‑binding
*REL6064,111,4290TG41.3% 89.8 / 13.7 63G496G (GGT→GGGmetLbifunctional aspartate kinase II/homoserine dehydrogenase II
*REL6062,376,3480TG40.6% 228.4 / 51.4 155T63P (ACC→CCC) folCbifunctional folylpolyglutamate synthase/ dihydrofolate synthase
*REL6062,213,0030AC40.6% 162.6 / 11.2 101V184G (GTG→GGG) fruK1‑phosphofructokinase
*REL6063,472,7000CG40.0% 103.0 / 87.4 100A290G (GCG→GGG) yhgApredicted transposase
*REL6062,822,1060GC38.8% 240.8 / 91.1 129pseudogene (62/198 nt)fucAL‑fuculose‑1‑phosphate aldolase; b2800_1
*REL6062,073,5230AC37.5% 226.5 / 52.3 120G432G (GGT→GGGasmApredicted assembly protein
*REL6062,676,6360TG37.2% 350.4 / 68.2 200V149G (GTG→GGG) smpBSsrA‑binding protein
*REL6063,378,8850TG36.6% 424.2 / 57.6 202T129P (ACC→CCC) rplB50S ribosomal protein L2
*REL606476,7460TG36.5% 166.6 / 18.7 85Q6H (CAA→CACfsrpredicted fosmidomycin efflux system
*REL6064,392,5560CT36.2% 217.7 / 89.5 152L43L (CTA→TTA) aidBisovaleryl CoA dehydrogenase
*REL6061,096,0320CG36.1% 211.3 / 97.6 119G148G (GGC→GGGputPproline:sodium symporter
*REL6064,169,2250TG35.9% 490.3 / 19.9 223intergenic (+41/‑172)rpoC/htrCDNA‑directed RNA polymerase subunit beta'/heat shock protein
*REL6061,118,2090GT35.4% 56.6 / 60.4 65intergenic (‑444/‑311)csgD/csgBDNA‑binding transcriptional activator in two‑component regulatory system/curlin nucleator protein, minor subunit in curli complex

Marginal new junction evidence (lowest skew 10 of 36 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 1506449213 (0.920)6 (0.030) 5/136 19.4 3.3% coding (72/792 nt) ydcC hypothetical protein
?REL606 = 3554988 162 (0.770)intergenic (+168/‑78) yrhC/yhhI b4552; predicted protein fragment/predicted transposase
* ? REL606 = 150630721 (0.090)6 (0.030) 5/136 19.4 5.5% intergenic (+169/‑71) ECB_01417/ydcC hypothetical protein/hypothetical protein
?REL606 = 1506442 188 (0.890)coding (65/792 nt) ydcC hypothetical protein
* ? REL606 = 71891482 (0.350)6 (0.030) 5/136 19.4 4.4% intergenic (+60/‑71) yhhI/ybfD predicted transposase; b3484_1/hypothetical protein
?REL606 = 1506442 188 (0.890)coding (65/792 nt) ydcC hypothetical protein
* ? REL606 = 717648203 (0.870)6 (0.030) 5/136 19.4 3.1% intergenic (+263/‑70) ECB_00664/yhhI hypothetical protein/predicted transposase; b3484_1
?REL606 = 1506442 188 (0.890)coding (65/792 nt) ydcC hypothetical protein
* ? REL606 570007 =75 (0.320)6 (0.030) 5/136 19.4 4.5% intergenic (‑71/+608) yhhI/ECB_00526 predicted transposase/hypothetical protein
?REL606 = 1506442 188 (0.890)coding (65/792 nt) ydcC hypothetical protein
* ? REL606 = 242742103 (0.440)6 (0.030) 5/136 19.4 4.1% intergenic (+360/‑71) ECB_00212/yhhI hypothetical protein/predicted transposase
?REL606 = 1506442 188 (0.890)coding (65/792 nt) ydcC hypothetical protein
* ? REL606 2039202 =108 (0.460)4 (0.020) 4/130 19.8 3.8% coding (493/747 nt) wbbA putative glycosyltransferase WbbA
?REL606 2039549 = 109 (0.540)coding (146/747 nt) wbbA putative glycosyltransferase WbbA
* ? REL606 = 2037392133 (0.570)5 (0.020) 4/136 20.2 3.7% coding (739/1176 nt) wzy O antigen polymerase (Wzy)
?REL606 = 2037686 138 (0.660)coding (445/1176 nt) wzy O antigen polymerase (Wzy)
* ? REL606 2679207 =219 (0.940)4 (0.020) 4/138 20.4 1.9% coding (279/1557 nt) ECB_02510 Recombinase
?REL606 2679381 = 213 (1.000)coding (453/1557 nt) ECB_02510 Recombinase
* ? REL606 = 2406673266 (1.150)5 (0.020) 4/138 20.4 1.9% intergenic (+363/‑3) fadL/yfdF long‑chain fatty acid outer membrane transporter/putative DNA modification enzyme; b2345_1
?REL606 = 2407104 280 (1.310)pseudogene (44/462 nt) yfdF putative DNA modification enzyme; b2345_1