breseq  version 0.32.0b  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 444,779 C→T 77.2% W440* (TGG→TAG)  cyoB ← cytochrome o ubiquinol oxidase subunit I
RA 788,752 C→A 12.1% G165C (GGC→TGC)  modF ← fused molybdate transporter subunits of ABC superfamily: ATP‑binding components
RA 1,191,676 C→T 27.4% H366H (CAC→CAT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,688 C→T 21.2% T370T (ACC→ACT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,701 T→C 23.8% L375L (TTA→CTA) ‡ icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,703 A→G 21.8% L375L (TTA→TTG) ‡ icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,207,564 A→T 13.8% intergenic (‑105/+595) iraM ← / ← ycgX RpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein
RA 2,007,748 C→T 17.4% P347S (CCG→TCG)  fliF → flagellar basal‑body MS‑ring and collar protein
RA 2,142,095 G→A 14.9% R314H (CGC→CAC)  yegD → Hsp70 chaperone family protein
JC JC 2,215,619 IS2 (+) +5 bp 33.3% intergenic (‑151/‑41) bglX ← / → dld beta‑D‑glucoside glucohydrolase, periplasmic/D‑lactate dehydrogenase, FAD‑binding, NADH independent
RA 2,305,991 G→T 57.2% Q80K (CAG→AAG)  ompC ← outer membrane porin protein C
RA 2,419,720 T→C 45.1% D183G (GAT→GGT)  hisJ ← histidine/lysine/arginine/ornithine transporter subunit
RA 2,420,353 A→G 67.6% intergenic (‑86/+135) hisJ ← / ← argT histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
RA 2,574,250 G→A 18.2% G419D (GGC→GAC)  tktB → transketolase 2, thiamine triphosphate‑binding
RA 3,165,217 A→T 15.3% intergenic (+25/+21) qseC → / ← ygiZ quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
RA 3,371,135 G→T 25.0% intergenic (‑356/+39) sspA ← / ← rpsI stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog/30S ribosomal subunit protein S9
JC 3,528,829:1 (GTTTTCAGCCAG)1→2 88.6% coding (399/1353 nt) envZ ← sensory histidine kinase in two‑component regulatory system with OmpR
JC 3,676,833 Δ3 bp 91.2% intergenic (‑26/+155) dctA ← / ← yhjK C4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase
RA 3,701,787 G→A 5.3% intergenic (‑722/+189) dppA ← / ← proK dipeptide transporter/tRNA‑Pro
RA 3,701,788 T→A 5.0% intergenic (‑723/+188) dppA ← / ← proK dipeptide transporter/tRNA‑Pro
RA 3,701,789 T→C 4.8% intergenic (‑724/+187) dppA ← / ← proK dipeptide transporter/tRNA‑Pro
RA 3,701,955 G→A 4.4% intergenic (‑890/+21) dppA ← / ← proK dipeptide transporter/tRNA‑Pro
RA 3,772,673 T→A 12.1% L87Q (CTA→CAA)  lldR → dual role activator/repressor for lldPRD operon

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =NA (NA)31 (1.340) 26/268 NT NA intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 1045234 =19 (0.820)28 (1.210) 23/268 NT 70.0% intergenic (‑34/‑55) gfcA/insA O‑antigen capsule production threonine‑rich inner membrane protein/IS1 repressor TnpA
?CP009273 = 1973123 5 (0.220)intergenic (‑669/‑111) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
* ? CP009273 1203246 =3 (0.130)12 (0.590) 12/236 NT 83.8% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 2 (0.100)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 12032613 (0.130)22 (1.080) 18/236 NT 90.5% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 2 (0.100)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 1973115 =5 (0.220)16 (0.690) 14/268 NT 76.2% intergenic (‑661/‑119) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E
* ? CP009273 = 2096424NA (NA)29 (1.250) 24/268 NT 74.4% noncoding (1/1195 nt) IS5 repeat region
?CP009273 = 2427745 10 (0.430)coding (476/660 nt) dedA DedA family inner membrane protein
* ? CP009273 2282398 =NA (NA)20 (0.860) 19/268 NT 66.7% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 2400257 = 10 (0.430)intergenic (‑137/‑783) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
* ? CP009273 2282398 =NA (NA)5 (0.220) 5/266 NT 31.2% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 3178254 = 11 (0.480)coding (56/291 nt) yqiC BMFP family putative fusogenic protein
* ? CP009273 = 457295415 (0.650)4 (0.180) 4/256 NT 19.4% intergenic (‑89/+112) hsdM/hsdR DNA methyltransferase M/pseudogene, premature stop codon, host restriction; endonuclease R
?CP009273 = 4573041 19 (0.860)intergenic (‑176/+25) hsdM/hsdR DNA methyltransferase M/pseudogene, premature stop codon, host restriction; endonuclease R