breseq version 0.32.0 revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
RA | 1,088,068 | A→G | I597T (ATC→ACC) | pgaB ← | poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein |
RA | 1,196,305 | C→T | R395C (CGT→TGT) | icd → | isocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase |
MC JC | 1,299,499 | Δ1,199 bp | intergenic (+254/‑485) | ychE → / → oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
MC JC | 1,619,080 | Δ35 bp | intergenic (‑172/‑6) | marC ← / → marR | UPF0056 family inner membrane protein/transcriptional repressor of multiple antibiotic resistance |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
RA | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
JC | 2,413,511 | Δ10 bp | coding (44‑53/1203 nt) | ackA → | acetate kinase A and propionate kinase 2 |
RA | 3,194,570 | (T)6→5 | intergenic (+47/+151) | yqiK → / ← sibD | PHB family membrane protein, function unknown/sRNA antisense regulator of toxic IbsD protein |
MC JC | 3,815,858 | Δ82 bp | [rph]–[rph] | [rph], [rph] | |
MC JC | 4,001,644 | Δ5 bp | coding (220‑224/951 nt) | corA → | magnesium/nickel/cobalt transporter |
MC JC | 4,049,990 | Δ12 bp | noncoding (93‑104/109 nt) | spf → | Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent |
RA | 4,184,542 | C→A | P1100Q (CCG→CAG) | rpoB → | RNA polymerase, beta subunit |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 3423696–3424534 | 3424534 | 1–839 | 35 [33] | [32] 34 | [rrfD]–[rrlD] | [rrfD], [rrlD] |
* | * | ÷ | NC_000913 | 4233746 | 4235446 | 1701 | 35 [32] | [33] 34 | pgi | pgi |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 257908 = | NA (NA) | 81 (1.130) | 61/284 | 0.1 | 95.3% | intergenic (+9/‑768) | crl/crl | pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers |
? | NC_000913 | = 1293039 | 4 (0.060) | intergenic (‑117/‑488) | hns/tdk | global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase | |||||
* | ? | NC_000913 | 458787 = | 3 (0.040) | 71 (1.010) | 62/278 | 0.1 | 96.0% | intergenic (+87/‑101) | clpX/lon | ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La |
? | NC_000913 | 608011 = | NA (NA) | intergenic (+23/‑54) | hokE/insL1 | toxic polypeptide, small/IS186 transposase | |||||
* | ? | NC_000913 | = 458799 | 4 (0.060) | 94 (1.350) | 74/276 | 0.0 | 96.0% | intergenic (+99/‑89) | clpX/lon | ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La |
? | NC_000913 | = 609347 | NA (NA) | intergenic (+170/+112) | insL1/entD | IS186 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex | |||||
* | ? | NC_000913 | 1207790 = | 32 (0.450) | 44 (0.690) | 42/252 | 0.5 | 64.5% | coding (290/630 nt) | stfP | e14 prophage, uncharacterized protein |
? | NC_000913 | 1209619 = | 20 (0.310) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1207805 | 34 (0.470) | 36 (0.570) | 32/252 | 1.2 | 59.0% | coding (305/630 nt) | stfP | e14 prophage, uncharacterized protein |
? | NC_000913 | = 1209602 | 20 (0.310) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related | |||||
* | ? | NC_000913 | 1293032 = | 5 (0.070) | 77 (1.070) | 64/284 | 0.0 | 93.9% | intergenic (‑110/‑495) | hns/tdk | global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase |
? | NC_000913 | = 1979270 | NA (NA) | intergenic (‑55/‑483) | insA/uspC | IS1 repressor TnpA/universal stress protein | |||||
* | ? | NC_000913 | 1979486 = | 2 (0.030) | 107 (1.490) | 70/284 | 0.0 | 98.2% | intergenic (‑271/‑267) | insA/uspC | IS1 repressor TnpA/universal stress protein |
? | NC_000913 | 2101749 = | NA (NA) | intergenic (‑5/+146) | wbbL/insH1 | pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator | |||||
* | ? | NC_000913 | = 1979489 | 5 (0.070) | 103 (1.440) | 55/284 | 0.2 | 95.4% | intergenic (‑274/‑264) | insA/uspC | IS1 repressor TnpA/universal stress protein |
? | NC_000913 | = 2290111 | NA (NA) | intergenic (‑32/+1) | insH1/yejO | IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system |