breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 13,586 C→A 5.2% T475N (ACC→AAC)  dnaK → chaperone Hsp70, with co‑chaperone DnaJ
RA 23,255 G→A 5.4% D289N (GAT→AAT)  ileS → isoleucyl‑tRNA synthetase
RA 27,186 G→A 5.1% V304M (GTG→ATG)  ispH → 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase, 4Fe‑4S protein
RA 53,376 G→A 6.7% P14L (CCC→CTC)  pdxA ← 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent
RA 53,379 T→C 6.5% E13G (GAG→GGG)  pdxA ← 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent
RA 83,587 A→C 13.0% intergenic (‑58/+35) leuA ← / ← leuL 2‑isopropylmalate synthase/leu operon leader peptide
RA 83,590 G→T 12.9% intergenic (‑61/+32) leuA ← / ← leuL 2‑isopropylmalate synthase/leu operon leader peptide
RA 87,884 T→A 5.0% intergenic (+36/‑144) ilvH → / → cra acetolactate synthase 3, small subunit, valine‑sensitive/transcriptional repressor‑activator for carbon metabolism
RA 164,620 C→T 7.0% intergenic (+86/‑110) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
RA 164,621 A→G 6.1% intergenic (+87/‑109) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
RA 191,720 G→A 5.3% intergenic (+12/‑135) tsf → / → pyrH translation elongation factor EF‑Ts/uridylate kinase
RA 191,728 T→C 5.9% intergenic (+20/‑127) tsf → / → pyrH translation elongation factor EF‑Ts/uridylate kinase
RA 191,729 G→T 5.3% intergenic (+21/‑126) tsf → / → pyrH translation elongation factor EF‑Ts/uridylate kinase
RA 191,730 A→C 5.3% intergenic (+22/‑125) tsf → / → pyrH translation elongation factor EF‑Ts/uridylate kinase
RA 191,731 G→A 6.3% intergenic (+23/‑124) tsf → / → pyrH translation elongation factor EF‑Ts/uridylate kinase
RA 191,739 T→C 6.5% intergenic (+31/‑116) tsf → / → pyrH translation elongation factor EF‑Ts/uridylate kinase
RA 210,017 C→T 5.6% I113I (ATC→ATT ldcC → lysine decarboxylase 2, constitutive
RA 256,449 T→C 8.3% intergenic (+14/‑78) gpt → / → frsA xanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase/fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
RA 256,454 C→G 7.1% intergenic (+19/‑73) gpt → / → frsA xanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase/fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
RA 256,459 G→A 7.3% intergenic (+24/‑68) gpt → / → frsA xanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase/fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 314,255 T→C 7.9% intergenic (‑450/‑102) ykgP ← / → eaeH pseudogene, oxidoreductase family/pseudogene, attaching and effacing protein homology;factor; Not classified
RA 314,258 G→A 7.9% intergenic (‑453/‑99) ykgP ← / → eaeH pseudogene, oxidoreductase family/pseudogene, attaching and effacing protein homology;factor; Not classified
RA 475,971 C→G 6.8% intergenic (+20/+11) ybaY → / ← ybaZ outer membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL
RA 475,972 C→G 6.8% intergenic (+21/+10) ybaY → / ← ybaZ outer membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL
RA 569,160 G→T 7.1% D87Y (GAC→TAC) ‡ ybcK → DLP12 prophage; putative recombinase
RA 569,161 A→C 7.0% D87A (GAC→GCC) ‡ ybcK → DLP12 prophage; putative recombinase
RA 605,022 C→G 6.1% D135H (GAT→CAT)  nfsB ← dihydropteridine reductase, NAD(P)H‑dependent, oxygen‑insensitive
RA 605,025 C→G 6.2% D134H (GAT→CAT)  nfsB ← dihydropteridine reductase, NAD(P)H‑dependent, oxygen‑insensitive
RA 657,531 T→G 9.8% intergenic (+30/+24) cspE → / ← flc constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein
RA 657,533 A→T 9.7% intergenic (+32/+22) cspE → / ← flc constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein
RA 657,535 C→A 9.7% intergenic (+34/+20) cspE → / ← flc constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein
RA 659,204 C→T 5.2% intergenic (+54/+47) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,205 A→T 5.4% intergenic (+55/+46) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,206 A→T 5.4% intergenic (+56/+45) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,207 A→G 5.4% intergenic (+57/+44) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 699,556 C→T 9.0% intergenic (‑379/+18) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 699,559 A→G 9.0% intergenic (‑382/+15) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 774,356 C→G 5.6% P16P (CCC→CCG ybgE → putative inner membrane protein in cydABX‑ybgE operon
RA 774,358 T→A 5.6% L17Q (CTG→CAG)  ybgE → putative inner membrane protein in cydABX‑ybgE operon
RA 805,312 G→T 8.6% E604* (GAG→TAG) ‡ ybhJ → aconitase family protein
RA 805,313 A→C 8.6% E604A (GAG→GCG) ‡ ybhJ → aconitase family protein
RA 892,455 G→C 7.4% V163V (GTG→GTC rimK → ribosomal protein S6 modification protein
RA 892,457 T→A 8.0% I164N (ATT→AAT)  rimK → ribosomal protein S6 modification protein
RA 892,459 G→C 8.0% D165H (GAC→CAC)  rimK → ribosomal protein S6 modification protein
RA 899,656 T→C 6.5% intergenic (+11/+188) rlmC → / ← artJ 23S rRNA m(5)U747 methyltransferase, SAM‑dependent/arginine ABC transporter periplasmic binding protein
RA 899,657 G→A 6.6% intergenic (+12/+187) rlmC → / ← artJ 23S rRNA m(5)U747 methyltransferase, SAM‑dependent/arginine ABC transporter periplasmic binding protein
RA 915,080 G→C 5.1% intergenic (‑223/+272) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 915,081 G→C 5.1% intergenic (‑224/+271) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 922,314:1 +C 6.1% intergenic (+21/+52) macB → / ← cspD macrolide ABC transporter peremase/ATPase/inhibitor of DNA replication, cold shock protein homolog
RA 940,751 T→C 5.5% intergenic (+31/‑208) serS → / → dmsA seryl‑tRNA synthetase/dimethyl sulfoxide reductase, anaerobic, subunit A
RA 940,754 G→A 6.7% intergenic (+34/‑205) serS → / → dmsA seryl‑tRNA synthetase/dimethyl sulfoxide reductase, anaerobic, subunit A
RA 987,560 G→C 8.1% intergenic (‑578/+25) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
RA 987,561 G→C 8.1% intergenic (‑579/+24) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
RA 1,015,912 T→A 6.7% intergenic (+30/+40) rmf → / ← fabA ribosome modulation factor/beta‑hydroxydecanoyl thioester dehydrase
RA 1,018,983 A→T 5.4% intergenic (+46/+30) matP → / ← ompA Ter macrodomain organizer matS‑binding protein/outer membrane protein A (3a;II*;G;d)
RA 1,018,986 A→T 5.4% intergenic (+49/+27) matP → / ← ompA Ter macrodomain organizer matS‑binding protein/outer membrane protein A (3a;II*;G;d)
RA 1,039,479 G→A 5.5% V62I (GTC→ATC) ‡ cbdB → cytochrome bd‑II oxidase, subunit II
RA 1,039,480 T→C 5.5% V62A (GTC→GCC) ‡ cbdB → cytochrome bd‑II oxidase, subunit II
RA 1,052,257 C→T 6.7% intergenic (+17/+32) gnsA → / ← yccM putative phosphatidylethanolamine synthesis regulator/putative 4Fe‑4S membrane protein
RA 1,052,260 T→C 6.3% intergenic (+20/+29) gnsA → / ← yccM putative phosphatidylethanolamine synthesis regulator/putative 4Fe‑4S membrane protein
RA 1,052,261 G→A 6.1% intergenic (+21/+28) gnsA → / ← yccM putative phosphatidylethanolamine synthesis regulator/putative 4Fe‑4S membrane protein
RA 1,052,264 A→G 5.9% intergenic (+24/+25) gnsA → / ← yccM putative phosphatidylethanolamine synthesis regulator/putative 4Fe‑4S membrane protein
RA 1,113,548 T→C 5.7% intergenic (+142/‑31) opgH → / → yceK OPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist/outer membrane integrity lipoprotein
RA 1,129,394 G→C 6.8% intergenic (+20/+20) murJ → / ← flgN putative lipid II flippase/export chaperone for FlgK and FlgL
RA 1,145,954 C→T 5.6% intergenic (+55/+57) rluC → / ← yceF 23S rRNA pseudouridine(955,2504,2580) synthase/m(7)GTP pyrophosphatase
RA 1,159,335 G→T 5.1% intergenic (+33/+27) ptsG → / ← fhuE fused glucose‑specific PTS enzymes: IIB component/IIC component/ferric‑rhodotorulic acid outer membrane transporter
RA 1,159,336 A→C 5.1% intergenic (+34/+26) ptsG → / ← fhuE fused glucose‑specific PTS enzymes: IIB component/IIC component/ferric‑rhodotorulic acid outer membrane transporter
RA 1,174,077 G→A 5.2% intergenic (‑113/+15) mfd ← / ← ycfT transcription‑repair coupling factor/inner membrane protein
RA 1,174,080 T→C 5.2% intergenic (‑116/+12) mfd ← / ← ycfT transcription‑repair coupling factor/inner membrane protein
RA 1,196,803 A→C 7.0% intergenic (+430/+64) icd → / ← ymfD isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase/e14 prophage; putative SAM‑dependent methyltransferase
RA 1,196,804 G→T 7.1% intergenic (+431/+63) icd → / ← ymfD isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase/e14 prophage; putative SAM‑dependent methyltransferase
RA 1,202,957 G→T 6.2% intergenic (‑24/‑67) cohE ← / → croE e14 prophage; repressor protein phage e14/e14 prophage; putative DNA‑binding transcriptional regulator
RA 1,202,959 T→A 6.1% intergenic (‑26/‑65) cohE ← / → croE e14 prophage; repressor protein phage e14/e14 prophage; putative DNA‑binding transcriptional regulator
RA 1,202,961 A→C 6.4% intergenic (‑28/‑63) cohE ← / → croE e14 prophage; repressor protein phage e14/e14 prophage; putative DNA‑binding transcriptional regulator
RA 1,273,229 A→T 8.3% intergenic (+27/+17) chaC → / ← ychN cation transport regulator/putative sulfur relay protein
JC JC 1,292,988 IS1 (+) +9 bp 32.3% intergenic (‑66/‑531) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
JC JC 1,293,037 IS1 (+) +9 bp 29.0% intergenic (‑115/‑482) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
RA 1,311,830 A→G 6.0% intergenic (+22/+18) tonB → / ← yciA membrane spanning protein in TonB‑ExbB‑ExbD transport complex/acyl‑CoA esterase
RA 1,311,831 C→T 6.0% intergenic (+23/+17) tonB → / ← yciA membrane spanning protein in TonB‑ExbB‑ExbD transport complex/acyl‑CoA esterase
RA 1,335,681 T→C 5.1% intergenic (+223/‑150) yciX → / → acnA uncharacterized protein/aconitate hydratase 1; aconitase A
RA 1,335,684 G→A 5.8% intergenic (+226/‑147) yciX → / → acnA uncharacterized protein/aconitate hydratase 1; aconitase A
RA 1,672,258 T→C 5.1% L100P (CTG→CCG)  ydgD → putative peptidase
RA 1,685,527 A→G 100% G354G (GGT→GGC fumC ← fumarate hydratase (fumarase C),aerobic Class II
RA 1,736,097 T→A 6.7% intergenic (+138/+24) sodB → / ← ydhP superoxide dismutase, Fe/putative MFS transporter, inner membrane protein
RA 1,736,100 T→A 6.7% intergenic (+141/+21) sodB → / ← ydhP superoxide dismutase, Fe/putative MFS transporter, inner membrane protein
RA 1,764,693 C→T 6.7% intergenic (‑307/+20) sufA ← / ← rydB Fe‑S cluster assembly protein/novel sRNA, function unknown
RA 1,795,227 A→T 9.1% intergenic (‑75/+26) btuC ← / ← ihfA vitamin B12 ABC transporter permease/integration host factor (IHF), DNA‑binding protein, alpha subunit
RA 1,795,228 G→C 9.1% intergenic (‑76/+25) btuC ← / ← ihfA vitamin B12 ABC transporter permease/integration host factor (IHF), DNA‑binding protein, alpha subunit
RA 1,795,229 A→T 9.4% intergenic (‑77/+24) btuC ← / ← ihfA vitamin B12 ABC transporter permease/integration host factor (IHF), DNA‑binding protein, alpha subunit
RA 1,797,388 G→T 9.3% P186Q (CCG→CAG)  pheT ← phenylalanine tRNA synthetase, beta subunit
RA 1,797,391 A→C 7.9% L185R (CTG→CGG)  pheT ← phenylalanine tRNA synthetase, beta subunit
RA 1,810,062 T→C 6.8% intergenic (+14/‑149) yniC → / → ydjM 2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA
RA 1,810,065 T→G 5.9% intergenic (+17/‑146) yniC → / → ydjM 2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA
RA 1,810,066 C→A 5.9% intergenic (+18/‑145) yniC → / → ydjM 2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA
RA 1,810,069 G→A 6.9% intergenic (+21/‑142) yniC → / → ydjM 2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA
RA 1,827,811 T→G 5.5% K150T (AAA→ACA)  astB ← succinylarginine dihydrolase
RA 1,827,816 C→A 5.3% L148L (CTG→CTT astB ← succinylarginine dihydrolase
RA 1,829,322 A→C 6.9% L138R (CTG→CGG)  astD ← succinylglutamic semialdehyde dehydrogenase
RA 1,829,328 C→G 6.8% G136A (GGC→GCC)  astD ← succinylglutamic semialdehyde dehydrogenase
RA 1,865,682 G→C 8.1% intergenic (‑46/+44) yeaE ← / ← mipA aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery
RA 1,901,947 T→G 29.6% intergenic (‑362/‑101) yoaE ← / → manX putative membrane protein/conserved protein/fused mannose‑specific PTS enzymes: IIA component/IIB component
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 1,985,112 C→G 6.1% intergenic (‑43/+27) araG ← / ← araF L‑arabinose ABC transporter ATPase/L‑arabinose ABC transporter periplasmic binding protein
RA 1,987,442 Δ1 bp 7.9% intergenic (+14/+65) ftnB → / ← yecJ ferritin B, putative ferrous iron reservoir/DUF2766 family protein
RA 1,987,450:1 +A 8.1% intergenic (+22/+57) ftnB → / ← yecJ ferritin B, putative ferrous iron reservoir/DUF2766 family protein
RA 2,090,122 A→G 6.6% intergenic (+76/‑70) hisL → / → hisG his operon leader peptide/ATP phosphoribosyltransferase
RA 2,090,124 C→G 6.7% intergenic (+78/‑68) hisL → / → hisG his operon leader peptide/ATP phosphoribosyltransferase
RA 2,090,126 C→T 6.5% intergenic (+80/‑66) hisL → / → hisG his operon leader peptide/ATP phosphoribosyltransferase
RA 2,145,313 C→A 100% S683Y (TCC→TAC)  yegE → putative diguanylate cyclase
RA 2,171,814 A→G 5.5% intergenic (‑87/+19) gatR ← / ← gatD pseudogene, repressor for gat operon; interrupted by IS3; split galactitol utilization operon repressor, fragment 2; split galactitol utilization operon repressor, interrupted/galactitol‑1‑phosphate dehydrogenase, Zn‑dependent and NAD(P)‑binding
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,305,085 A→G 15.4% intergenic (+692/+23) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,086 C→G 15.8% intergenic (+693/+22) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,087 C→T 15.8% intergenic (+694/+21) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,311,616 C→T 5.3% intergenic (‑82/+30) apbE ← / ← ompC putative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C
RA 2,318,095 C→G 100% L594F (TTG→TTC rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
RA 2,383,956 A→T 6.1% intergenic (+32/+39) elaD → / ← yfbK protease, capable of cleaving an AMC‑ubiquitin model substrate/Von Willebrand factor domain putative lipoprotein
RA 2,383,959 A→T 6.3% intergenic (+35/+36) elaD → / ← yfbK protease, capable of cleaving an AMC‑ubiquitin model substrate/Von Willebrand factor domain putative lipoprotein
RA 2,457,128 T→A 5.4% M678L (ATG→TTG)  fadJ ← enoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
RA 2,457,131 T→A 5.4% I677L (ATA→TTA)  fadJ ← enoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
RA 2,457,138 C→G 6.2% V674V (GTG→GTC fadJ ← enoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
RA 2,462,677 A→T 5.9% intergenic (+31/‑335) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein
RA 2,462,678 T→A 5.7% intergenic (+32/‑334) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein
RA 2,462,679 A→T 5.3% intergenic (+33/‑333) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein
RA 2,536,246 C→A 100% P138Q (CCG→CAG)  crr → glucose‑specific enzyme IIA component of PTS
RA 2,637,439 C→A 5.3% intergenic (‑83/+35) der ← / ← bamB GTPase; multicopy suppressor of ftsJ/BamABCDE complex OM biogenesis lipoprotein
RA 2,646,746 C→T 5.8% A194T (GCA→ACA)  pbpC ← penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein
RA 2,706,134 Δ1 bp 5.2% coding (991/1800 nt) lepA ← back‑translocating elongation factor EF4, GTPase
RA 2,714,238 G→A 7.3% intergenic (+8/+201) srmB → / ← yfiE ATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator
RA 2,714,244 T→C 7.3% intergenic (+14/+195) srmB → / ← yfiE ATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator
RA 2,714,249 G→A 7.5% intergenic (+19/+190) srmB → / ← yfiE ATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator
RA 2,724,095 C→A 7.4% intergenic (+13/‑33) pssA → / → yfiM phosphatidylserine synthase; CDP‑diacylglycerol‑serine O‑phosphatidyltransferase/putative lipoprotein
RA 2,724,097 T→A 7.3% intergenic (+15/‑31) pssA → / → yfiM phosphatidylserine synthase; CDP‑diacylglycerol‑serine O‑phosphatidyltransferase/putative lipoprotein
RA 2,724,099 T→G 6.4% intergenic (+17/‑29) pssA → / → yfiM phosphatidylserine synthase; CDP‑diacylglycerol‑serine O‑phosphatidyltransferase/putative lipoprotein
RA 2,736,911 C→T 6.0% intergenic (+28/‑243) bamD → / → raiA BamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit
RA 2,736,913 T→A 6.0% intergenic (+30/‑241) bamD → / → raiA BamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit
RA 2,736,914 T→A 6.1% intergenic (+31/‑240) bamD → / → raiA BamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit
RA 2,736,916 A→G 6.1% intergenic (+33/‑238) bamD → / → raiA BamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit
RA 2,768,614 A→C 5.0% intergenic (+41/‑51) yfjP → / → yfjQ CP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein
RA 2,778,123 G→T 5.2% intergenic (+341/+23) ypjF → / ← ypjA CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter
RA 2,778,124 A→C 5.0% intergenic (+342/+22) ypjF → / ← ypjA CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter
RA 2,800,515 C→A 5.4% intergenic (+40/‑208) ygaM → / → nrdH putative membrane‑anchored DUF883 family ribosome‑binding protein/hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein
RA 2,800,517 T→A 5.4% intergenic (+42/‑206) ygaM → / → nrdH putative membrane‑anchored DUF883 family ribosome‑binding protein/hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein
RA 2,814,175 T→A 5.2% intergenic (+21/+43) emrB → / ← luxS multidrug efflux system protein/S‑ribosylhomocysteine lyase
RA 2,845,792 C→T 5.4% R227H (CGT→CAT)  hycE ← hydrogenase 3, large subunit
RA 2,906,608 G→C 5.6% intergenic (+25/+35) ygcG → / ← eno TPM domain protein, putative phosphatase/enolase
RA 2,918,017 C→T 5.0% intergenic (+204/+28) barA → / ← gudD hybrid sensory histidine kinase, in two‑component regulatory system with UvrY/D‑glucarate dehydratase 1
RA 2,918,019 G→T 5.1% intergenic (+206/+26) barA → / ← gudD hybrid sensory histidine kinase, in two‑component regulatory system with UvrY/D‑glucarate dehydratase 1
RA 2,918,020 A→C 5.0% intergenic (+207/+25) barA → / ← gudD hybrid sensory histidine kinase, in two‑component regulatory system with UvrY/D‑glucarate dehydratase 1
RA 2,918,022 A→G 5.2% intergenic (+209/+23) barA → / ← gudD hybrid sensory histidine kinase, in two‑component regulatory system with UvrY/D‑glucarate dehydratase 1
RA 2,972,652 A→T 5.8% intergenic (+15/+17) tas → / ← lplT putative NADP(H)‑dependent aldo‑keto reductase/lysophospholipid transporter
RA 2,972,653 A→T 5.8% intergenic (+16/+16) tas → / ← lplT putative NADP(H)‑dependent aldo‑keto reductase/lysophospholipid transporter
RA 3,020,510 G→T 9.2% intergenic (+21/‑30) ssnA → / → ygfM putative chlorohydrolase/aminohydrolase/putative oxidoreductase
RA 3,020,511 C→G 9.1% intergenic (+22/‑29) ssnA → / → ygfM putative chlorohydrolase/aminohydrolase/putative oxidoreductase
RA 3,020,512 A→C 9.2% intergenic (+23/‑28) ssnA → / → ygfM putative chlorohydrolase/aminohydrolase/putative oxidoreductase
RA 3,057,140 A→G 5.8% intergenic (+152/+38) sibC → / ← serA sRNA antisense regulator of toxic IbsC protein/D‑3‑phosphoglycerate dehydrogenase
RA 3,057,145 G→C 5.8% intergenic (+157/+33) sibC → / ← serA sRNA antisense regulator of toxic IbsC protein/D‑3‑phosphoglycerate dehydrogenase
RA 3,057,150 C→T 5.0% intergenic (+162/+28) sibC → / ← serA sRNA antisense regulator of toxic IbsC protein/D‑3‑phosphoglycerate dehydrogenase
RA 3,059,703 A→T 5.2% intergenic (‑2/‑50) yqfE ← / → argP pseudogene, LysR family/transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
RA 3,059,704 A→T 5.2% intergenic (‑3/‑49) yqfE ← / → argP pseudogene, LysR family/transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
RA 3,098,515 G→C 7.9% intergenic (+112/+43) yggW → / ← yggM HemN family putative oxidoreductase/DUF1202 family putative secreted protein
RA 3,098,518 C→T 7.8% intergenic (+115/+40) yggW → / ← yggM HemN family putative oxidoreductase/DUF1202 family putative secreted protein
RA 3,098,519 A→G 7.8% intergenic (+116/+39) yggW → / ← yggM HemN family putative oxidoreductase/DUF1202 family putative secreted protein
RA 3,098,522 G→C 7.6% intergenic (+119/+36) yggW → / ← yggM HemN family putative oxidoreductase/DUF1202 family putative secreted protein
RA 3,107,875 A→G 5.8% I427I (ATT→ATC) ‡ speC ← ornithine decarboxylase, constitutive
RA 3,107,876 A→C 5.8% I427S (ATT→AGT) ‡ speC ← ornithine decarboxylase, constitutive
RA 3,107,879 G→T 5.7% A426D (GCT→GAT) ‡ speC ← ornithine decarboxylase, constitutive
RA 3,107,880 C→T 5.8% A426T (GCT→ACT) ‡ speC ← ornithine decarboxylase, constitutive
RA 3,156,590 C→A 8.5% intergenic (+72/‑33) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
RA 3,156,591 T→G 8.4% intergenic (+73/‑32) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
RA 3,180,497 Δ1 bp 8.6% coding (77/1161 nt) ygiC → ATP‑Grasp family ATPase
RA 3,180,505 G→T 10.4% E29* (GAG→TAG) ‡ ygiC → ATP‑Grasp family ATPase
RA 3,180,506 A→C 10.2% E29A (GAG→GCG) ‡ ygiC → ATP‑Grasp family ATPase
RA 3,180,513:1 +A 8.0% coding (93/1161 nt) ygiC → ATP‑Grasp family ATPase
RA 3,183,345:1 +T 7.7% intergenic (+22/+468) zupT → / ← ribB zinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
RA 3,183,350 Δ1 bp 7.4% intergenic (+27/+463) zupT → / ← ribB zinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
RA 3,202,003 G→C 5.6% G38A (GGC→GCC)  cca → fused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase
RA 3,202,009 A→T 5.8% Q40L (CAG→CTG)  cca → fused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase
RA 3,202,012 A→T 5.8% Q41L (CAG→CTG)  cca → fused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase
RA 3,239,609 T→G 6.1% intergenic (+64/‑335) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter
RA 3,318,079 A→T 5.2% intergenic (‑73/+134) rimP ← / ← metY ribosome maturation factor for 30S subunits/tRNA‑Met
RA 3,328,182 A→T 6.0% intergenic (+99/+57) yhbY → / ← greA RNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor
RA 3,328,183 T→A 5.6% intergenic (+100/+56) yhbY → / ← greA RNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor
RA 3,328,184 A→T 5.5% intergenic (+101/+55) yhbY → / ← greA RNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor
RA 3,330,415 Δ1 bp 5.1% intergenic (+19/+167) dacB → / ← obgE D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair
RA 3,330,419:1 +T 5.1% intergenic (+23/+163) dacB → / ← obgE D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair
RA 3,334,703 A→G 5.9% intergenic (+22/‑206) ispB → / → sfsB octaprenyl diphosphate synthase/malPQ operon transcriptional activator
RA 3,334,704 G→C 6.0% intergenic (+23/‑205) ispB → / → sfsB octaprenyl diphosphate synthase/malPQ operon transcriptional activator
RA 3,334,705 C→T 6.1% intergenic (+24/‑204) ispB → / → sfsB octaprenyl diphosphate synthase/malPQ operon transcriptional activator
RA 3,374,260 G→A 5.5% A77V (GCA→GTA)  nanR ← sialic acid‑inducible nan operon repressor
RA 3,407,293 T→A 5.7% intergenic (+27/‑82) accC → / → yhdT acetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein
RA 3,407,294 T→A 5.8% intergenic (+28/‑81) accC → / → yhdT acetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein
RA 3,407,295 T→A 5.7% intergenic (+29/‑80) accC → / → yhdT acetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein
RA 3,439,588 G→C 6.7% intergenic (‑79/+28) yhdN ← / ← rplQ DUF1992 family protein/50S ribosomal subunit protein L17
RA 3,439,589 G→C 6.7% intergenic (‑80/+27) yhdN ← / ← rplQ DUF1992 family protein/50S ribosomal subunit protein L17
RA 3,476,585 G→A 5.1% intergenic (‑145/+22) yheO ← / ← fkpA putative PAS domain‑containing DNA‑binding transcriptional regulator/FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
RA 3,476,586 T→C 6.1% intergenic (‑146/+21) yheO ← / ← fkpA putative PAS domain‑containing DNA‑binding transcriptional regulator/FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,708,595 G→A 5.3% intergenic (‑890/+21) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
RA 3,708,596 T→A 5.2% intergenic (‑891/+20) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
RA 3,708,597 T→C 5.2% intergenic (‑892/+19) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
RA 3,714,163:1 +T 8.2% coding (2232/2334 nt) bisC ← biotin sulfoxide reductase
RA 3,714,168 Δ1 bp 7.9% coding (2227/2334 nt) bisC ← biotin sulfoxide reductase
RA 3,781,051 C→G 6.4% intergenic (+34/‑164) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,891,502 C→G 5.5% intergenic (+19/‑113) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein
RA 3,891,504 T→A 5.2% intergenic (+21/‑111) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein
RA 3,891,506 C→G 5.3% intergenic (+23/‑109) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein
RA 4,007,054 C→T 6.4% C397C (TGC→TGT recQ → ATP‑dependent DNA helicase
RA 4,015,333 A→T 5.5% intergenic (+19/+21) metE → / ← ysgA 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase/putative carboxymethylenebutenolidase
RA 4,060,261 G→T 5.7% intergenic (+31/‑186) typA → / → yihL GTP‑binding protein/putative DNA‑binding transcriptional regulator
RA 4,060,264 A→C 5.7% intergenic (+34/‑183) typA → / → yihL GTP‑binding protein/putative DNA‑binding transcriptional regulator
RA 4,062,371 A→T 5.9% N42I (AAT→ATT)  yihN → MFS transporter family protein
RA 4,116,937 T→C 84.4% K96E (AAG→GAG)  glpK ← glycerol kinase
RA 4,122,658 G→C 5.0% P228A (CCA→GCA)  ftsN ← essential cell division protein
RA 4,188,501 A→G 22.1% D1051G (GAC→GGC)  rpoC → RNA polymerase, beta prime subunit
RA 4,235,581 T→A 5.7% intergenic (+174/‑325) pgi → / → yjbE glucosephosphate isomerase/extracellular polysaccharide production threonine‑rich protein
RA 4,274,740 G→A 7.4% intergenic (+79/+20) ssb → / ← yjcB single‑stranded DNA‑binding protein/putative inner membrane protein
RA 4,274,742 T→A 7.7% intergenic (+81/+18) ssb → / ← yjcB single‑stranded DNA‑binding protein/putative inner membrane protein
RA 4,274,744 T→C 7.5% intergenic (+83/+16) ssb → / ← yjcB single‑stranded DNA‑binding protein/putative inner membrane protein
RA 4,289,094 T→A 6.9% I444N (ATT→AAT)  nrfA → nitrite reductase, formate‑dependent, cytochrome
RA 4,296,060 C→T 20.0% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,312,080 G→C 6.0% intergenic (‑38/+21) alsB ← / ← alsR D‑allose ABC transporter periplasmic binding protein/d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR
RA 4,312,083 G→C 5.9% intergenic (‑41/+18) alsB ← / ← alsR D‑allose ABC transporter periplasmic binding protein/d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR
RA 4,312,089 A→G 5.8% intergenic (‑47/+12) alsB ← / ← alsR D‑allose ABC transporter periplasmic binding protein/d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR
RA 4,314,227 A→T 7.5% intergenic (+30/+117) yjdP → / ← phnP putative periplasmic protein/5‑phospho‑alpha‑D‑ribosyl 1,2‑cyclic phosphate phosphodiesterase
RA 4,314,233 A→T 7.4% intergenic (+36/+111) yjdP → / ← phnP putative periplasmic protein/5‑phospho‑alpha‑D‑ribosyl 1,2‑cyclic phosphate phosphodiesterase
RA 4,314,239 A→T 5.2% intergenic (+42/+105) yjdP → / ← phnP putative periplasmic protein/5‑phospho‑alpha‑D‑ribosyl 1,2‑cyclic phosphate phosphodiesterase
RA 4,336,974 A→T 5.2% S20T (TCG→ACG)  adiC ← arginine:agmatine antiporter
RA 4,362,528 G→A 5.3% intergenic (‑175/+23) yjdQ ← / ← pheU pseudogene, P4‑like integrase remnant/tRNA‑Phe
RA 4,362,529 T→C 5.1% intergenic (‑176/+22) yjdQ ← / ← pheU pseudogene, P4‑like integrase remnant/tRNA‑Phe
RA 4,381,396 G→C 7.1% P308R (CCG→CGG)  frdA ← anaerobic fumarate reductase catalytic and NAD/flavoprotein subunit
RA 4,381,403 A→C 7.1% S306A (TCC→GCC)  frdA ← anaerobic fumarate reductase catalytic and NAD/flavoprotein subunit
RA 4,392,617 C→G 5.2% noncoding (35/76 nt) glyY → tRNA‑Gly
RA 4,426,592 T→A 6.0% intergenic (+35/+36) rplI → / ← yjfZ 50S ribosomal subunit protein L9/uncharacterized protein
RA 4,479,012 A→T 10.4% intergenic (+123/+22) rraB → / ← yjgM protein inhibitor of RNase E/GNAT family putative N‑acetyltransferase
RA 4,479,016 G→C 10.0% intergenic (+127/+18) rraB → / ← yjgM protein inhibitor of RNase E/GNAT family putative N‑acetyltransferase
RA 4,479,020 A→T 9.4% intergenic (+131/+14) rraB → / ← yjgM protein inhibitor of RNase E/GNAT family putative N‑acetyltransferase
RA 4,520,500 Δ1 bp 5.8% intergenic (‑176/+171) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
RA 4,520,504:1 +T 5.7% intergenic (‑180/+167) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
RA 4,549,932 T→A 5.3% intergenic (+222/+21) fimH → / ← gntP minor component of type 1 fimbriae/fructuronate transporter
RA 4,614,383 G→A 6.2% intergenic (+123/+297) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423753–3424234 3424557–3424238 5–805 44 [41] [43] 44 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 3988386 (0.890)6 (0.060) 6/252 NT 6.8% coding (504/1143 nt) caiA crotonobetaine reductase subunit II, FAD‑binding
?NC_000913 = 39906 83 (0.900)coding (481/1143 nt) caiA crotonobetaine reductase subunit II, FAD‑binding
* ? NC_000913 = 6660999 (1.030)8 (0.080) 7/260 NT 7.8% noncoding (45/248 nt) REP6 (repetitive extragenic palindromic) element; contains 6 REP sequences REP6 (repetitive extragenic palindromic) element; contains 6 REP sequences
?NC_000913 = 66643 91 (0.950)noncoding (79/248 nt) REP6 (repetitive extragenic palindromic) element; contains 6 REP sequences REP6 (repetitive extragenic palindromic) element; contains 6 REP sequences
* ? NC_000913 = 19066599 (1.030)9 (0.100) 5/250 NT 8.7% intergenic (+66/‑192) rpsB/tsf 30S ribosomal subunit protein S2/translation elongation factor EF‑Ts
?NC_000913 = 190685 95 (1.030)intergenic (+86/‑172) rpsB/tsf 30S ribosomal subunit protein S2/translation elongation factor EF‑Ts
* ? NC_000913 274620 =NA (NA)4 (0.040) 4/244 NT NA noncoding (530/1195 nt) IS5 repeat region
?NC_000913 274663 = NA (NA)noncoding (487/1195 nt) IS5 repeat region
* ? NC_000913 = 291401NA (NA)6 (0.060) 6/262 NT 12.2% noncoding (1/768 nt) IS1 repeat region
?NC_000913 1293015 = 43 (0.450)intergenic (‑93/‑512) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 = 381669NA (NA)5 (0.050) 5/248 NT NA noncoding (410/1331 nt) IS2 repeat region
?NC_000913 = 381695 NA (NA)noncoding (436/1331 nt) IS2 repeat region
* ? NC_000913 1207790 =32 (0.330)53 (0.630) 37/230 NT 61.2% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 39 (0.460)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780527 (0.280)38 (0.450) 34/230 NT 54.8% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 39 (0.460)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 1293124 =75 (0.780)5 (0.050) 5/262 NT 6.2% intergenic (‑202/‑403) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1300693 NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 1293196 =88 (0.910)8 (0.080) 7/262 NT 8.3% intergenic (‑274/‑331) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1300693 NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 12994980 (0.000)96 (1.030) 77/254 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 3620220NA (NA)3 (0.030) 3/250 NT 100% coding (1029/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 = 3620255 0 (0.000)coding (1064/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 3721045 =124 (1.290)274 (2.870) 135/260 NT 69.7% noncoding (413/1443 nt) IS150 repeat region
?NC_000913 = 3839774 115 (1.200)coding (220/819 nt) nlpA cytoplasmic membrane lipoprotein‑28
* ? NC_000913 4136837 =59 (0.610)4 (0.040) 4/250 NT 6.8% coding (730/906 nt) yijE EamA‑like transporter family protein
?NC_000913 4136866 = 54 (0.590)coding (759/906 nt) yijE EamA‑like transporter family protein