breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 586,138 C→A intergenic (‑505/+9) ompT ← / ← envY outer membrane protease VII (outer membrane protein 3b)/DNA‑binding transcriptional activator EnvY
JC JC 742,852 IS1 (+) +9 bp coding (26‑34/744 nt) ybgI → radiation resistance protein YbgI
RA 795,056 T→C intergenic (+134/‑33) acrZ → / → modA multidrug efflux pump accessory protein AcrZ/molybdate ABC transporter periplasmic binding protein
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,316,047 C→A intergenic (‑12/+369) yciG ← / ← trpA protein YciG/tryptophan synthase subunit alpha
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,880,107 G→T intergenic (‑159/+14) yeaR ← / ← leuE DUF1971 domain‑containing protein YeaR/leucine exporter
RA 1,890,808 G→T Q154K (CAA→AAA)  tsaB ← N(6)‑L‑threonylcarbamoyladenine synthase, TsaB subunit
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,045,513 C→A G192G (GGC→GGA yeeJ → inverse autotransporter adhesin
RA 2,066,191 G→T intergenic (‑427/+114) cobU ← / ← insH‑6 cobinamide‑P guanylyltransferase/cobinamide kinase/CP4‑44 prophage; IS5 transposase and trans‑activator
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,336,669 G→T intergenic (‑25/+124) yfaA ← / ← gyrA DUF2138 domain‑containing protein YfaA/DNA gyrase subunit A
RA 2,440,802 G→T D268E (GAC→GAA fabB ← beta‑ketoacyl‑[acyl carrier protein] synthase I
RA 2,454,367 G→T pseudogene (635/2646 nt) yfcU ← putative outer membrane usher protein YfcU
RA 2,504,229 C→A R86L (CGC→CTC)  fryA ← putative PTS multiphosphoryl transfer protein FryA
RA 2,527,982 C→A G60W (GGG→TGG)  ypeB ← PF12843 family protein YpeB
RA 2,649,324 C→A P988P (CCG→CCT yfhM ← alpha2‑macroglobulin
RA 2,719,644 C→A P141Q (CCG→CAG)  yfiP → conserved protein YfiP
RA 3,068,916 G→T intergenic (‑108/+31) argO ← / ← mscS L‑arginine exporter/small conductance mechanosensitive channel MscS
RA 3,272,933 G→T H209N (CAC→AAC)  garL ← alpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase
RA 3,301,945 G→T R154L (CGG→CTG)  yhbU → putative peptidase YhbU
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,699,034 C→A A274D (GCC→GAC)  bcsG → cellulose phosphoethanolamine transferase
RA 3,732,374 C→A S58S (TCC→TCA xylG → xylose ABC transporter ATP binding subunit
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,993,671 C→A T840K (ACG→AAG)  cyaA → adenylate cyclase
RA 4,159,226 C→A P246Q (CCG→CAG)  oxyR → DNA‑binding transcriptional dual regulator OxyR
RA 4,218,270 G→T A388S (GCC→TCC)  aceA → isocitrate lyase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102968 3104081 1114 48 [47] [45] 48 mutY mutY
* * ÷ NC_000913 3423760–3424234 3424526–3424238 5–767 49 [46] [43] 48 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =143 (0.840)67 (0.440) 47/244 1.3 33.2% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 143 (0.950)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 1207805143 (0.840)40 (0.270) 29/244 3.0 22.9% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 143 (0.950)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)247 (1.490) 102/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =312 (1.830)296 (1.740) 107/274 0.0 65.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 5 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)188 (1.100) 78/276 0.4 96.4% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 7 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA