breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 281,830 C→A A52S (GCT→TCT)  yagA ← CP4‑6 prophage; integrase core domain‑containing protein YagA
RA 298,096 C→A A211S (GCC→TCC)  paoD ← molybdenum cofactor insertion chaperone for PaoABC
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,061,902 C→A A35E (GCG→GAG)  torD → trimethylamine‑N‑oxide reductase‑specific chaperone
RA 1,291,179 G→T S313I (AGC→ATC)  rssB → regulator of RpoS
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,368,220 G→T A48S (GCG→TCG)  pspA → phage shock protein A
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,294,130 G→T L259M (CTG→ATG)  ccmF ← holocytochrome c synthetase membrane subunit CcmF
RA 2,367,561 +G coding (491/969 nt) arnC → undecaprenyl‑phosphate 4‑deoxy‑4‑formamido‑L‑arabinose transferase
RA 2,376,989 Δ1 bp coding (604/759 nt) menH ← 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
RA 2,651,105 C→A D395Y (GAT→TAT)  yfhM ← alpha2‑macroglobulin
RA 2,771,942 G→T S71S (TCC→TCA yfjU ← CP4‑57 prophage; ArsC family protein YfjU
RA 2,933,154 G→T Q179K (CAA→AAA)  fucA ← L‑fuculose‑phosphate aldolase
RA 2,940,353 C→T W297* (TGG→TGA rlmM ← 23S rRNA 2'‑O‑ribose C2498 methyltransferase
RA 3,089,446 C→A T388N (ACC→AAC)  galP → galactose:H(+) symporter
RA 3,337,667 C→A A75A (GCG→GCT mlaC ← intermembrane phospholipid transport system ‑ periplasmic binding protein
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,766,621 G→T L95F (TTG→TTT yibA → putative lyase containing HEAT‑repeat
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,194,562 C→A G513V (GGG→GTG)  thiC ← phosphomethylpyrimidine synthase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,374,924 C→A R245L (CGG→CTG)  epmB ← lysine 2,3‑aminomutase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 433006 433076 71 3 [1] [2] 3 nrdR NrdR transcriptional repressor
* * ÷ NC_000913 3103015 3104043 1029 4 [2] [0] 4 mutY adenine DNA glycosylase
* * ÷ NC_000913 3423776–3424231 3424525–3424239 9–750 3 [1] [2] 4 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =6 (0.270)28 (1.430) 26/246 0.0 82.0% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 7 (0.360)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12078056 (0.270)18 (0.920) 16/246 0.3 74.5% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 7 (0.360)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 3363001 =41 (1.850)45 (2.040) 28/276 0.0 68.9% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)24 (1.080) 18/278 0.3 92.3% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.090)coding (3907/4134 nt) rhsA rhs element protein RhsA