breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 170,124 C→A A116E (GCG→GAG)  fhuC → iron(III) hydroxamate ABC transporter ATP binding subunit
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 344,101 G→T intergenic (+168/‑75) yahK → / → yahL aldehyde reductase, NADPH‑dependent/uncharacterized protein YahL
RA 492,598 C→A R169R (CGC→CGA
R169R (CGC→CGA
dnaX →
dnaX →
DNA polymerase III subunit tau
DNA polymerase III subunit tau
JC JC 523,084 IS5 (+) +4 bp intergenic (+254/‑174) ybbP → / → rhsD putative ABC transporter membrane subunit YbbP/protein RhsD
JC 548,472 +AGGG coding (116/1260 nt) ylbE → DUF1116 domain‑containing protein YlbE
RA 553,788 A→G D51D (GAT→GAC lpxH ← UDP‑2,3‑diacylglucosamine diphosphatase
RA 658,216 C→A pseudogene (186/788 nt) ybeM → deamidated glutathione amidase, N‑terminal fragment
RA 733,974 A→G T67A (ACT→GCT)  ybfB → uncharacterized protein YbfB
RA 766,659 G→T G226C (GGC→TGC)  mngA → 2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,389,688 T→G G253G (GGT→GGG ycjG → L‑Ala‑D/L‑Glu epimerase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,339,065 G→C A119G (GCA→GGA)  gyrA ← DNA gyrase subunit A
RA 2,512,652 G→T L19I (CTC→ATC)  mntH ← Mn(2(+))/Fe(2(+)): H(+) symporter MntH
RA 2,758,577 G→T N8K (AAC→AAA yfjH ← CP4‑57 prophage; protein YfjH
RA 3,120,251 C→A V343V (GTG→GTT glcA ← glycolate/lactate:H(+) symporter GlcA
RA 3,204,045 G→T G29G (GGC→GGA bacA ← undecaprenyl pyrophosphate phosphatase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,623,761 G→T L121L (CTG→CTT yhhH → PF15631 family protein YhhH
RA 3,642,135 C→A A425D (GCT→GAT)  dtpB → dipeptide/tripeptide:H(+) symporter DtpB
RA 3,778,459 T→C V354A (GTC→GCC)  lldP → lactate/glycolate:H(+) symporter LldP
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,381,952 C→A E123* (GAG→TAG)  frdA ← fumarate reductase flavoprotein subunit
RA 4,423,288 C→A A148E (GCG→GAG)  ulaE → L‑ribulose‑5‑phosphate 3‑epimerase UlaE
RA 4,627,818 (A)5→4 coding (504/1233 nt) nadR → DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196289 1211428 15140 13 [11] [9] 12 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 2518104 2518178 75 12 [11] [11] 12 [alaX]–[alaW] [alaX],[alaW]
* * ÷ NC_000913 3102986 3104051 1066 12 [10] [11] 13 [mutY] [mutY]
* * ÷ NC_000913 3423782–3424234 3424525–3424238 5–744 12 [10] [10] 14 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3363001 =60 (1.110)59 (1.100) 40/274 0.2 66.1% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)61 (1.130) 49/276 0.1 96.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA