breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 9,085 G→A 50.3% R685H (CGT→CAT)  gyrA → DNA topoisomerase (ATP‑hydrolyzing) subunit A
RA 18,890 C→G 93.9% N182K (AAC→AAG yybT → DHH subfamily phosphodiesterase
RA 39,378 C→A 100% D586Y (GAT→TAT)  mecA ← penicillin binding protein 2 prime
RA 76,969 G→A 100% intergenic (+1027/‑107) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 110,660 T→A 11.7% I173I (ATT→ATA USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 111,512 A→G 31.9% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,516 C→A 31.3% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,560 T→C 9.6% R210R (CGT→CGC USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 195,286 T→A 100% intergenic (‑97/‑82) USA300HOU_0183 ← / → USA300HOU_0184 hypothetical protein/hypothetical protein
RA 264,463 G→A 5.5% W226* (TGG→TGA glpQ1 → possible glycerophosphodiester phosphodiesterase
RA 396,540 G→A 100% A208T (GCA→ACA)  USA300HOU_0364 → possible lipoprotein
RA 512,643 A→G 51.5% intergenic (+711/‑150) recR → / → rrs1 recombination protein RecR/16S ribosomal RNA
RA 535,338 A→T 5.3% intergenic (+4909/‑816) veg → / → rplY hypothetical protein/ribosomal protein L25
RA 607,884 G→A 100% R209* (CGA→TGA)  dck ← deoxynucleoside kinase
RA 629,681 C→A 100% L84I (CTA→ATA)  hxlA → possible 3‑hexulose‑6‑phosphate synthase
RA 665,392 T→G 100% L79* (TTA→TGA)  iolS → possible acetytransferase
RA 707,910 A→G 100% intergenic (‑1/‑121) mntB ← / → sirR ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator
RA 807,436:1 +T 10.5% intergenic (‑634/‑167) USA300HOU_0753 ← / → nrdI DMT superfamily drug/metabolite transporter/ribonucleotide‑diphosphate reductase subunit gamma
RA 1,100,582 A→G 6.2% intergenic (‑146/+184) USA300HOU_1033 ← / ← pdf1 hypothetical protein/formylmethionine deformylase
RA 1,120,712 G→C 7.5% R67P (CGC→CCC)  ylaN → hypothetical protein
RA 1,233,504 T→A 100% V470D (GTT→GAT)  prkC → non‑specific serine/threonine protein kinase
RA 1,269,458 G→T 7.7% D173Y (GAT→TAT)  gid → glucose‑inhibited division protein Gid
RA 1,274,208 C→T 100% S204L (TCA→TTA)  codY → CodY family transcriptional regulator
RA 1,416,458 A→T 100% I216N (ATT→AAT)  oppD ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,435,075 T→A 15.8% G144G (GGT→GGA dapD → 2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase
RA 1,440,085 G→T 100% intergenic (‑45/‑891) cspA ← / → xpaC cold shock protein CspA/5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC
RA 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,646,189 G→C 100% A155G (GCA→GGA)  pepP ← M24B family Xaa‑Pro dipeptidase
RA 1,679,145 G→T 12.4% R4R (CGC→CGA recO ← recombination protein RecO
RA 1,744,362 G→A 100% H104Y (CAC→TAC)  apt ← adenine phosphoribosyltransferase
RA 1,761,889 C→A 100% D388Y (GAT→TAT)  folC ← tetrahydrofolate synthase
RA 1,857,867 C→T 10.0% L169L (TTG→TTA USA300HOU_1728 ← hypothetical protein
RA 1,909,486:1 +T 100% coding (293/303 nt) USA300HOU_1771 → hypothetical membrane protein
RA 1,963,768 C→G 100% V365L (GTA→CTA)  hemG ← protoporphyrinogen oxidase
RA 2,010,363 C→T 100% C146Y (TGT→TAT)  ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,028,269 G→C 100% P126A (CCA→GCA)  yvqF ← hypothetical membrane protein
RA 2,211,305 A→G 8.1% intergenic (‑31/+515) USA300HOU_2086 ← / ← yidC hypothetical protein/hypothetical membrane protein
RA 2,240,343 C→A 5.8% E339* (GAA→TAA)  murZ ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
RA 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,289,695 C→A 7.0% G298C (GGT→TGT)  arg ← arginase
RA 2,310,618 A→T 7.3% G82G (GGT→GGA USA300HOU_2169 ← possible iron (Fe3+) ABC superfamily ATP binding cassette transporter, binding protein
RA 2,352,815 G→A 100% A92V (GCA→GTA)  rpsI ← ribosomal protein S9
RA 2,405,489 G→T 94.8% intergenic (‑127/‑124) USA300HOU_2268 ← / → ureA UT family urea transporter/urease gamma subunit
RA 2,454,626 A→T 100% L48L (CTA→CTT USA300HOU_2315 → hypothetical membrane protein
RA 2,468,697 C→T 9.7% E39K (GAA→AAA)  USA300HOU_2331 ← possible esterase
RA 2,532,298 A→T 100% T15T (ACA→ACT murN → FemAB family peptidoglycan biosynthesis protein
RA 2,604,421 C→T 6.5% H430Y (CAT→TAT)  mtrF → AbgT family p‑aminobenzoyl‑glutamate transporter
RA 2,648,469 T→A 8.1% G79G (GGA→GGT lltB ← MFS family major facilitator transporter
RA 2,679,725 G→A 100% W70* (TGG→TAG)  cbpD → possible secretory antigen
JC 2,709,677 Δ12 bp 94.7% coding (1239‑1250/1812 nt) oatA ← possible acetyltransferase
RA 2,852,714 G→T 8.4% intergenic (+68/‑170) USA300HOU_2695 → / → 2486 hypothetical protein/DASS family divalent anion:sodium (Na+) symporter

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =92 (1.230)67 (1.300)
+40 bp
42/180 NT 51.4% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA
* ? CP000730 = 170226759 (0.790)4 (0.060) 4/238 NT 6.4% intergenic (‑2/+38) comEA/yqeM possible competence protein comEA/hypothetical protein
?CP000730 = 1702290 64 (0.940)intergenic (‑25/+15) comEA/yqeM possible competence protein comEA/hypothetical protein